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The map label for this gene is prfA

Identifier: 150008712

GI number: 150008712

Start: 2486190

End: 2487299

Strand: Direct

Name: prfA

Synonym: BDI_2102

Alternate gene names: 150008712

Gene position: 2486190-2487299 (Clockwise)

Preceding gene: 150008711

Following gene: 150008713

Centisome position: 51.67

GC content: 49.19

Gene sequence:

>1110_bases
ATGGCCGAAAATAGTTTACTTGGTAAATTAGACGGATTAGTGAGCCGTTTCGAGGAAGTCGGTACCCTTATTACCGACCC
GGCGGTGATCGCCGACATGAAACGGTTCGTAAAACTGAACAAAGAGTACCGTGATTTGGAGAAAATTGTGGGAGCTCGTT
CGGAATACGTGAAAGTATTGAACGGGATCGAGGAAGCGAAAGCCTTGCTTGAGTCCGAGCAAGATCCGGAAATGCGGGAA
ATGGCCCGTGAGGAGCTGGATACCTGCAACGAACGTATTCCCGCTTTGGAAGAAGAGATAAAATTATTGCTCGTTCCCGC
TGATCCGCAAGACGACAAGAACGCGATCGTTGAGATACGAGGAGGAACGGGAGGTGACGAAGCCGCCCTTTTCGCCGGTG
ATCTATATCGTATGTACGTGAAGTATTGCGAACTGAAGGGATGGAAAATAAGCGTGTCTAGCTTTAGCGAAGGCTCGTCC
GGCGGTTTTAAGGAAATCATCTTCACGGTATCGGGCGAGAAAGTATACGGAACGTTGAAGTATGAGTCCGGTGTACACCG
TGTACAGCGTGTACCGGCTACGGAGACGCAAGGACGTGTACATACCTCCGCCGCTACAGTTGCCGTATTGCCTGAGGCGG
ATGAATTTGATGTTGAGATTAATGAAGGGGAGATTAAATGGGATACCTTCCGCTCCGGTGGGGCCGGTGGTCAGAACGTA
AACAAGGTGGAATCAGGTGTCCGTCTGCGTTATGTCTGGAAGAACCCCATTACAGGTGTGAGTGAGGAAATCTTGATCGA
ATGTACGGAGACACGAGACCAACCGAAGAACAAAGAAAGGGCGCTTACCCGTCTTCGTTCGTTTATTTATGACAAGGAAC
ACCAGAAATACTTGGACGATATCGCCAGCAAGCGTAAGACCATGGTATCTACCGGTGACCGCTCCGCGAAGATCCGTACG
TACAATTACCCGCAAGGGCGTATCACGGATCACCGTATCAATTACACGATCTATAATCTTTCAGCCTTTATGGACGGTGA
AATTCAAGATTGCTTGGATCACCTGATCGTAGCGGAGAACGCTGAGCGACTGAAAGAGTCTGAATTGTAA

Upstream 100 bases:

>100_bases
TGGTATTGATGCGCAAATCGTGGGCTTCGTAGAGGAAGCTGATAAAAATGAATTGATTATCGAAAGCGAGAAAGGTCGCT
TCACCTATTAATAAAGATAA

Downstream 100 bases:

>100_bases
ACACATCCAATCATTTCATACTGAATAAGAAAATGAACAAACAACAGCTATTCGAGAATATTAAAAAGAAACAATCCTTC
CTTTGTGTCGGATTAGATAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MAENSLLGKLDGLVSRFEEVGTLITDPAVIADMKRFVKLNKEYRDLEKIVGARSEYVKVLNGIEEAKALLESEQDPEMRE
MAREELDTCNERIPALEEEIKLLLVPADPQDDKNAIVEIRGGTGGDEAALFAGDLYRMYVKYCELKGWKISVSSFSEGSS
GGFKEIIFTVSGEKVYGTLKYESGVHRVQRVPATETQGRVHTSAATVAVLPEADEFDVEINEGEIKWDTFRSGGAGGQNV
NKVESGVRLRYVWKNPITGVSEEILIECTETRDQPKNKERALTRLRSFIYDKEHQKYLDDIASKRKTMVSTGDRSAKIRT
YNYPQGRITDHRINYTIYNLSAFMDGEIQDCLDHLIVAENAERLKESEL

Sequences:

>Translated_369_residues
MAENSLLGKLDGLVSRFEEVGTLITDPAVIADMKRFVKLNKEYRDLEKIVGARSEYVKVLNGIEEAKALLESEQDPEMRE
MAREELDTCNERIPALEEEIKLLLVPADPQDDKNAIVEIRGGTGGDEAALFAGDLYRMYVKYCELKGWKISVSSFSEGSS
GGFKEIIFTVSGEKVYGTLKYESGVHRVQRVPATETQGRVHTSAATVAVLPEADEFDVEINEGEIKWDTFRSGGAGGQNV
NKVESGVRLRYVWKNPITGVSEEILIECTETRDQPKNKERALTRLRSFIYDKEHQKYLDDIASKRKTMVSTGDRSAKIRT
YNYPQGRITDHRINYTIYNLSAFMDGEIQDCLDHLIVAENAERLKESEL
>Mature_368_residues
AENSLLGKLDGLVSRFEEVGTLITDPAVIADMKRFVKLNKEYRDLEKIVGARSEYVKVLNGIEEAKALLESEQDPEMREM
AREELDTCNERIPALEEEIKLLLVPADPQDDKNAIVEIRGGTGGDEAALFAGDLYRMYVKYCELKGWKISVSSFSEGSSG
GFKEIIFTVSGEKVYGTLKYESGVHRVQRVPATETQGRVHTSAATVAVLPEADEFDVEINEGEIKWDTFRSGGAGGQNVN
KVESGVRLRYVWKNPITGVSEEILIECTETRDQPKNKERALTRLRSFIYDKEHQKYLDDIASKRKTMVSTGDRSAKIRTY
NYPQGRITDHRINYTIYNLSAFMDGEIQDCLDHLIVAENAERLKESEL

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=304, Percent_Identity=40.1315789473684, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI34577120, Length=316, Percent_Identity=35.7594936708861, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI166795305, Length=205, Percent_Identity=39.5121951219512, Blast_Score=161, Evalue=9e-40,
Organism=Escherichia coli, GI1787462, Length=361, Percent_Identity=47.3684210526316, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI2367172, Length=352, Percent_Identity=32.6704545454545, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17542784, Length=321, Percent_Identity=34.2679127725857, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=343, Percent_Identity=36.1516034985423, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI19921226, Length=329, Percent_Identity=39.209726443769, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_PARD8 (A6LDR9)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303455.1
- ProteinModelPortal:   A6LDR9
- SMR:   A6LDR9
- STRING:   A6LDR9
- GeneID:   5307251
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2102
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- ProtClustDB:   PRK00591
- BioCyc:   PDIS435591:BDI_2102-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41607; Mature: 41476

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENSLLGKLDGLVSRFEEVGTLITDPAVIADMKRFVKLNKEYRDLEKIVGARSEYVKVL
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHH
NGIEEAKALLESEQDPEMREMAREELDTCNERIPALEEEIKLLLVPADPQDDKNAIVEIR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEE
GGTGGDEAALFAGDLYRMYVKYCELKGWKISVSSFSEGSSGGFKEIIFTVSGEKVYGTLK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEECCEEEEEEE
YESGVHRVQRVPATETQGRVHTSAATVAVLPEADEFDVEINEGEIKWDTFRSGGAGGQNV
EHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCEEEEEECCCCCCCCCCH
NKVESGVRLRYVWKNPITGVSEEILIECTETRDQPKNKERALTRLRSFIYDKEHQKYLDD
HHHHCCCEEEEEECCCCCCCCHHHEEEEHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
IASKRKTMVSTGDRSAKIRTYNYPQGRITDHRINYTIYNLSAFMDGEIQDCLDHLIVAEN
HHHHHHHHHCCCCCCCEEEEEECCCCCEECEEEEEEEEEEEECCCCCHHHHHHHHHHHHC
AERLKESEL
HHHHHHCCC
>Mature Secondary Structure 
AENSLLGKLDGLVSRFEEVGTLITDPAVIADMKRFVKLNKEYRDLEKIVGARSEYVKVL
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHH
NGIEEAKALLESEQDPEMREMAREELDTCNERIPALEEEIKLLLVPADPQDDKNAIVEIR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEE
GGTGGDEAALFAGDLYRMYVKYCELKGWKISVSSFSEGSSGGFKEIIFTVSGEKVYGTLK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEECCEEEEEEE
YESGVHRVQRVPATETQGRVHTSAATVAVLPEADEFDVEINEGEIKWDTFRSGGAGGQNV
EHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCEEEEEECCCCCCCCCCH
NKVESGVRLRYVWKNPITGVSEEILIECTETRDQPKNKERALTRLRSFIYDKEHQKYLDD
HHHHCCCEEEEEECCCCCCCCHHHEEEEHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
IASKRKTMVSTGDRSAKIRTYNYPQGRITDHRINYTIYNLSAFMDGEIQDCLDHLIVAEN
HHHHHHHHHCCCCCCCEEEEEECCCCCEECEEEEEEEEEEEECCCCCHHHHHHHHHHHHC
AERLKESEL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA