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The map label for this gene is lacZ [H]

Identifier: 150008590

GI number: 150008590

Start: 2338971

End: 2341475

Strand: Reverse

Name: lacZ [H]

Synonym: BDI_1976

Alternate gene names: 150008590

Gene position: 2341475-2338971 (Counterclockwise)

Preceding gene: 150008592

Following gene: 150008585

Centisome position: 48.67

GC content: 49.66

Gene sequence:

>2505_bases
ATGAAAAGAACAAGTTTCGCTATTTTGATCGGTTTGTTTTGCCTTTTCCCTGCGCTTTTTTCTCAAGTACGCAGTGAGTT
CCTGTTGGAAAAGAATTGGAAGTTCACCCGGGAAGACCGCCCGGAGTTTATCAATCCTACTTTTAACGATAGCAAATGGC
AATCCGTGACTGTTCCGCATGATTGGGCGATTTACGGCCCTTTCAGCTCTCAGAATGATAAGCAAGAGGTCGCTATCGCC
CAAGACGGACAAACGGAGGCCATGGAACATGCCGGCCGAACCGGAGGTTTGCCTTTCGTGGGAGTCGGCTGGTACCGGAC
CGAGTTTGAGGTTCCTCAATTTACGGATGGGAAGCGTGCCACGATCCTGTTCGACGGTGCGATGAGCCATGCTAAGGTCT
ACGTGAACGGACAAGAGGTTGGATATTGGCCGTATGGCTATAATTCTTTCTATTTCGATATCACGAAACAACTCATGCCG
GGGAAAAAGAACACCTTGGCGGTTCGCCTCGAGAACTTGCCGGAATCCTCCCGTTGGTATCCGGGAGCGGGATTATATAG
GAATGTACATGTAATCGTGACGGAAAACGCCTATATCCCGGTCTGGGGAACTTATATTACGACCCCGACCGCCACGAAGG
AATTTGCGAAAGTCAATGTACAGACGAAAATCGTACTGCCGGAAGGTGCCGATGCCAGCAAGTATAGCGTGAAAACCAGT
GTATGGAATCCGAACGGGCAGAAGTTGACGGAGCAGACTATGCCTCTCTCACAGATTAAGTACAATGATAATAGCCTCTC
CCAAGAGTTTATCATCCAGACCCCGACTTTATGGTCGCCGGATATGCCCGCCCTGTATTCTGCGGAGACTCGTCTCTATG
AGGGCGATCAACTGAAAGATATATATACGACCCCGTTCGGTATCCGTTCGATCGAGATCATTCCGAATAAAGGCTTTTTC
TTGAATGGTGAGAAGACGGTATTCAAAGGCGTTTGTAATCACCATGACTTAGGCCCGTTAGGGGCTGCCGTCAATGATGC
CGCTATCCGTCGTCAGATCCGGATCTTGAAGGACATGGGATGCAACGCTATCCGTACTTCCCATAATATGCCGGCTCCCG
AGTTGGTAAAGGCTTGCGATGAGATGGGTATGATGTTGATGGCAGAGAGCTTCGACGAATGGAACAAGGCGAAATGTGCG
AACGGCTATAATTTGATTTTCGATGAATGGGTGGAGAAAGACCTCGTGAATCTGGTACATCATTACCGGAATAACCCGAG
CGTCGTGATGTGGTGCGTCGGCAACGAGGTGCCTAACCAATGGGATGAGAGCGGTTGTAAGATCTCCAAGTTCTTGCAGG
ATATCTGCCATCGGGAAGACCCGACTCGTCCGGTGACGCAAGGTATGGATGCCCCGGATGCCGTCGTGAATAATAATATG
GCGGCGGTGATGGATGTGGCGGGATTCAATTATCGTCCGTTCCGTTATCAAGTGAATTATAAGAAACTGCCTCAGCAAAT
AATCTTGGGAAGCGAGACGGCCTCCACGGTTAGCTCACGAGGGGTTTATAAATTCCCGGTAGAGCGTAAGGCGATGGCTG
TCTATGAGGATCACCAAAGTTCCTCGTACGACGTGGAGCATTGCAACTGGTCTAATCTGCCGGAAGACGATTTTATCCAG
CATGAGGACCTTCCGTATTGTATCGGGGAATTCGTATGGACAGGATTTGATTATCTGGGCGAGCCTACTCCTTATTATAC
GAATTGGCCAAGCCATAGCTCCTTGTTCGGTATTATCGATTTGGCGGGTATCCCCAAGGATCGTTACTATCTATACCGTA
GTCATTGGAACAAAAGCGCCAAGACCTTGCATATCCTGCCGCATTGGACATGGCCGGGACGTGAGGGCGAGGTGACACCC
GTATTCGTTTATACCAATTATCCTTCCGCCGAATTGTTTATCAACGGAAAGAGCCAAGGGAAACGTACGAAAGATCTAAG
CATCGGTATCGATAGTAGTTATACCGAAGCGGCTCAAAAGAGTTTCGAGCGCCAGAAACGATATCGTTTGATGTGGATGG
ATACCAAATACGAGCCGGGAACTTTGAAGGTGGTCGCTTATGACAAGGAGGGTAACGCTGTGGCCGAGGAGGAGGTTCAT
ACCGCAGGGAAGCCGCATCATATTGAGTTATCTGCCGATCGTAACCAATTGACGGCTGACGGAAAAGACCTTTCTTTCAT
CAATGTTAGAGTCGTAGACAAGAACGGTAACCTTTGTCCGGATGATACCCGCCAGATCCAGTTTAAGGTACGTGGTGCCG
GAGCCTACCGTGCGGCGGCAAATGGAAATAGCGCCAGTCTGGAACTCTTCCATCTTCCGGAAATGAAACTGTTTAGCGGG
CAACTTACCGCTATCGTGCGGACGGCCGAGGAACCGGGGACGATCTATTTCGAGGCTTCCGCACCGGGAGTAAAAGGGGC
GACCTTGGAATTGATCAGTAAATAA

Upstream 100 bases:

>100_bases
ATTGTAACAACATGCTGTGCAATTCTTTTGATTTAGTTTGTATTGGAGACAAAAATACTTATTTTTGCTCCGCTATGAAT
AACAAAAACACAAAAAACGA

Downstream 100 bases:

>100_bases
GCTTGATTATCATATAACGCAAACCGCGCGATTCAAGTCCGGAGACGGCTGTGAATCGTGCGGCTTTTTTATACGATATT
CCCGAATACATCAAAGGTAA

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 834; Mature: 834

Protein sequence:

>834_residues
MKRTSFAILIGLFCLFPALFSQVRSEFLLEKNWKFTREDRPEFINPTFNDSKWQSVTVPHDWAIYGPFSSQNDKQEVAIA
QDGQTEAMEHAGRTGGLPFVGVGWYRTEFEVPQFTDGKRATILFDGAMSHAKVYVNGQEVGYWPYGYNSFYFDITKQLMP
GKKNTLAVRLENLPESSRWYPGAGLYRNVHVIVTENAYIPVWGTYITTPTATKEFAKVNVQTKIVLPEGADASKYSVKTS
VWNPNGQKLTEQTMPLSQIKYNDNSLSQEFIIQTPTLWSPDMPALYSAETRLYEGDQLKDIYTTPFGIRSIEIIPNKGFF
LNGEKTVFKGVCNHHDLGPLGAAVNDAAIRRQIRILKDMGCNAIRTSHNMPAPELVKACDEMGMMLMAESFDEWNKAKCA
NGYNLIFDEWVEKDLVNLVHHYRNNPSVVMWCVGNEVPNQWDESGCKISKFLQDICHREDPTRPVTQGMDAPDAVVNNNM
AAVMDVAGFNYRPFRYQVNYKKLPQQIILGSETASTVSSRGVYKFPVERKAMAVYEDHQSSSYDVEHCNWSNLPEDDFIQ
HEDLPYCIGEFVWTGFDYLGEPTPYYTNWPSHSSLFGIIDLAGIPKDRYYLYRSHWNKSAKTLHILPHWTWPGREGEVTP
VFVYTNYPSAELFINGKSQGKRTKDLSIGIDSSYTEAAQKSFERQKRYRLMWMDTKYEPGTLKVVAYDKEGNAVAEEEVH
TAGKPHHIELSADRNQLTADGKDLSFINVRVVDKNGNLCPDDTRQIQFKVRGAGAYRAAANGNSASLELFHLPEMKLFSG
QLTAIVRTAEEPGTIYFEASAPGVKGATLELISK

Sequences:

>Translated_834_residues
MKRTSFAILIGLFCLFPALFSQVRSEFLLEKNWKFTREDRPEFINPTFNDSKWQSVTVPHDWAIYGPFSSQNDKQEVAIA
QDGQTEAMEHAGRTGGLPFVGVGWYRTEFEVPQFTDGKRATILFDGAMSHAKVYVNGQEVGYWPYGYNSFYFDITKQLMP
GKKNTLAVRLENLPESSRWYPGAGLYRNVHVIVTENAYIPVWGTYITTPTATKEFAKVNVQTKIVLPEGADASKYSVKTS
VWNPNGQKLTEQTMPLSQIKYNDNSLSQEFIIQTPTLWSPDMPALYSAETRLYEGDQLKDIYTTPFGIRSIEIIPNKGFF
LNGEKTVFKGVCNHHDLGPLGAAVNDAAIRRQIRILKDMGCNAIRTSHNMPAPELVKACDEMGMMLMAESFDEWNKAKCA
NGYNLIFDEWVEKDLVNLVHHYRNNPSVVMWCVGNEVPNQWDESGCKISKFLQDICHREDPTRPVTQGMDAPDAVVNNNM
AAVMDVAGFNYRPFRYQVNYKKLPQQIILGSETASTVSSRGVYKFPVERKAMAVYEDHQSSSYDVEHCNWSNLPEDDFIQ
HEDLPYCIGEFVWTGFDYLGEPTPYYTNWPSHSSLFGIIDLAGIPKDRYYLYRSHWNKSAKTLHILPHWTWPGREGEVTP
VFVYTNYPSAELFINGKSQGKRTKDLSIGIDSSYTEAAQKSFERQKRYRLMWMDTKYEPGTLKVVAYDKEGNAVAEEEVH
TAGKPHHIELSADRNQLTADGKDLSFINVRVVDKNGNLCPDDTRQIQFKVRGAGAYRAAANGNSASLELFHLPEMKLFSG
QLTAIVRTAEEPGTIYFEASAPGVKGATLELISK
>Mature_834_residues
MKRTSFAILIGLFCLFPALFSQVRSEFLLEKNWKFTREDRPEFINPTFNDSKWQSVTVPHDWAIYGPFSSQNDKQEVAIA
QDGQTEAMEHAGRTGGLPFVGVGWYRTEFEVPQFTDGKRATILFDGAMSHAKVYVNGQEVGYWPYGYNSFYFDITKQLMP
GKKNTLAVRLENLPESSRWYPGAGLYRNVHVIVTENAYIPVWGTYITTPTATKEFAKVNVQTKIVLPEGADASKYSVKTS
VWNPNGQKLTEQTMPLSQIKYNDNSLSQEFIIQTPTLWSPDMPALYSAETRLYEGDQLKDIYTTPFGIRSIEIIPNKGFF
LNGEKTVFKGVCNHHDLGPLGAAVNDAAIRRQIRILKDMGCNAIRTSHNMPAPELVKACDEMGMMLMAESFDEWNKAKCA
NGYNLIFDEWVEKDLVNLVHHYRNNPSVVMWCVGNEVPNQWDESGCKISKFLQDICHREDPTRPVTQGMDAPDAVVNNNM
AAVMDVAGFNYRPFRYQVNYKKLPQQIILGSETASTVSSRGVYKFPVERKAMAVYEDHQSSSYDVEHCNWSNLPEDDFIQ
HEDLPYCIGEFVWTGFDYLGEPTPYYTNWPSHSSLFGIIDLAGIPKDRYYLYRSHWNKSAKTLHILPHWTWPGREGEVTP
VFVYTNYPSAELFINGKSQGKRTKDLSIGIDSSYTEAAQKSFERQKRYRLMWMDTKYEPGTLKVVAYDKEGNAVAEEEVH
TAGKPHHIELSADRNQLTADGKDLSFINVRVVDKNGNLCPDDTRQIQFKVRGAGAYRAAANGNSASLELFHLPEMKLFSG
QLTAIVRTAEEPGTIYFEASAPGVKGATLELISK

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=553, Percent_Identity=24.4122965641953, Blast_Score=108, Evalue=3e-23,
Organism=Escherichia coli, GI48994920, Length=477, Percent_Identity=24.1090146750524, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1787903, Length=405, Percent_Identity=24.9382716049383, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1786539, Length=389, Percent_Identity=24.4215938303342, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17510825, Length=444, Percent_Identity=24.5495495495495, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24651745, Length=524, Percent_Identity=24.618320610687, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922582, Length=428, Percent_Identity=25, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24655438, Length=428, Percent_Identity=25, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI62471735, Length=428, Percent_Identity=25, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 94503; Mature: 94503

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTSFAILIGLFCLFPALFSQVRSEFLLEKNWKFTREDRPEFINPTFNDSKWQSVTVPH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
DWAIYGPFSSQNDKQEVAIAQDGQTEAMEHAGRTGGLPFVGVGWYRTEFEVPQFTDGKRA
CEEEECCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCEEECCEEEEEECCCCCCCCCEE
TILFDGAMSHAKVYVNGQEVGYWPYGYNSFYFDITKQLMPGKKNTLAVRLENLPESSRWY
EEEEECCCCEEEEEECCCEEEEECCCCCCEEEEEHHHHCCCCCCEEEEEEECCCCCCCCC
PGAGLYRNVHVIVTENAYIPVWGTYITTPTATKEFAKVNVQTKIVLPEGADASKYSVKTS
CCCCCEEEEEEEEECCEEEEEEEEEEECCCCCCHHHEEEEEEEEEECCCCCCCCEEEEEE
VWNPNGQKLTEQTMPLSQIKYNDNSLSQEFIIQTPTLWSPDMPALYSAETRLYEGDQLKD
EECCCCCCHHHHCCCHHHEEECCCCCCCEEEEECCCCCCCCCCCEECCCCEEECCCCCCE
IYTTPFGIRSIEIIPNKGFFLNGEKTVFKGVCNHHDLGPLGAAVNDAAIRRQIRILKDMG
EECCCCCCEEEEEECCCCEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
CNAIRTSHNMPAPELVKACDEMGMMLMAESFDEWNKAKCANGYNLIFDEWVEKDLVNLVH
CCEEECCCCCCHHHHHHHHHHHCHHHHHHHHCHHCHHHCCCCCCCHHHHHHHHHHHHHHH
HYRNNPSVVMWCVGNEVPNQWDESGCKISKFLQDICHREDPTRPVTQGMDAPDAVVNNNM
HHCCCCCEEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHCCCC
AAVMDVAGFNYRPFRYQVNYKKLPQQIILGSETASTVSSRGVYKFPVERKAMAVYEDHQS
EEEEEECCCCCCCEEEEECHHHCCHHHEECCCHHHHHHCCCEEECCCCCCEEEEEECCCC
SSYDVEHCNWSNLPEDDFIQHEDLPYCIGEFVWTGFDYLGEPTPYYTNWPSHSSLFGIID
CCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHCHHHCCCCCCCCCCCCCCCCEEEEEE
LAGIPKDRYYLYRSHWNKSAKTLHILPHWTWPGREGEVTPVFVYTNYPSAELFINGKSQG
ECCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEEECCCCC
KRTKDLSIGIDSSYTEAAQKSFERQKRYRLMWMDTKYEPGTLKVVAYDKEGNAVAEEEVH
CCCCEEEECCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHH
TAGKPHHIELSADRNQLTADGKDLSFINVRVVDKNGNLCPDDTRQIQFKVRGAGAYRAAA
HCCCCEEEEEECCCCEEECCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEECCCEEEEEC
NGNSASLELFHLPEMKLFSGQLTAIVRTAEEPGTIYFEASAPGVKGATLELISK
CCCCCEEEEEECCCCHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECC
>Mature Secondary Structure
MKRTSFAILIGLFCLFPALFSQVRSEFLLEKNWKFTREDRPEFINPTFNDSKWQSVTVPH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
DWAIYGPFSSQNDKQEVAIAQDGQTEAMEHAGRTGGLPFVGVGWYRTEFEVPQFTDGKRA
CEEEECCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCEEECCEEEEEECCCCCCCCCEE
TILFDGAMSHAKVYVNGQEVGYWPYGYNSFYFDITKQLMPGKKNTLAVRLENLPESSRWY
EEEEECCCCEEEEEECCCEEEEECCCCCCEEEEEHHHHCCCCCCEEEEEEECCCCCCCCC
PGAGLYRNVHVIVTENAYIPVWGTYITTPTATKEFAKVNVQTKIVLPEGADASKYSVKTS
CCCCCEEEEEEEEECCEEEEEEEEEEECCCCCCHHHEEEEEEEEEECCCCCCCCEEEEEE
VWNPNGQKLTEQTMPLSQIKYNDNSLSQEFIIQTPTLWSPDMPALYSAETRLYEGDQLKD
EECCCCCCHHHHCCCHHHEEECCCCCCCEEEEECCCCCCCCCCCEECCCCEEECCCCCCE
IYTTPFGIRSIEIIPNKGFFLNGEKTVFKGVCNHHDLGPLGAAVNDAAIRRQIRILKDMG
EECCCCCCEEEEEECCCCEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
CNAIRTSHNMPAPELVKACDEMGMMLMAESFDEWNKAKCANGYNLIFDEWVEKDLVNLVH
CCEEECCCCCCHHHHHHHHHHHCHHHHHHHHCHHCHHHCCCCCCCHHHHHHHHHHHHHHH
HYRNNPSVVMWCVGNEVPNQWDESGCKISKFLQDICHREDPTRPVTQGMDAPDAVVNNNM
HHCCCCCEEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHCCCC
AAVMDVAGFNYRPFRYQVNYKKLPQQIILGSETASTVSSRGVYKFPVERKAMAVYEDHQS
EEEEEECCCCCCCEEEEECHHHCCHHHEECCCHHHHHHCCCEEECCCCCCEEEEEECCCC
SSYDVEHCNWSNLPEDDFIQHEDLPYCIGEFVWTGFDYLGEPTPYYTNWPSHSSLFGIID
CCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHCHHHCCCCCCCCCCCCCCCCEEEEEE
LAGIPKDRYYLYRSHWNKSAKTLHILPHWTWPGREGEVTPVFVYTNYPSAELFINGKSQG
ECCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEEECCCCC
KRTKDLSIGIDSSYTEAAQKSFERQKRYRLMWMDTKYEPGTLKVVAYDKEGNAVAEEEVH
CCCCEEEECCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHH
TAGKPHHIELSADRNQLTADGKDLSFINVRVVDKNGNLCPDDTRQIQFKVRGAGAYRAAA
HCCCCEEEEEECCCCEEECCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEECCCEEEEEC
NGNSASLELFHLPEMKLFSGQLTAIVRTAEEPGTIYFEASAPGVKGATLELISK
CCCCCEEEEEECCCCHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA