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The map label for this gene is bioB [H]

Identifier: 150008528

GI number: 150008528

Start: 2273423

End: 2274703

Strand: Direct

Name: bioB [H]

Synonym: BDI_1914

Alternate gene names: 150008528

Gene position: 2273423-2274703 (Clockwise)

Preceding gene: 150008527

Following gene: 150008529

Centisome position: 47.25

GC content: 53.16

Gene sequence:

>1281_bases
ATGACAACAACCGAACTTTTACAATACGACCGGGATCATCTATGGCATCCCTACACCTCGACCATCGATCCACTTCCCGT
CTATCCGGTATCCAAAGCGGAGGGCGTAACGATAACCCTTGCGGACGGCAGGGAGCTAATAGACGGAATGTCTTCTTGGT
GGGCGGCAGTCCACGGATACAACCATCCGGTACTTAACGCGGCGGCTAAAGCCCAATTGGATAAGATGAGTCATATCATG
TTCGGGGGATTCACGCACGAGCCGGCTGTCGAGTTGACCGCTAAATTATTACCGCTATTGCCCCCTTCTCTAGAAAAGAT
ATTCCTAGCGGATAGCGGCTCGGTCGCCGTGGAGGTAGCCTTAAAGATGGCGATCCAATACTGGCAGTCCAAAGGCATAC
CGGGAAAACATACCTTCGCCACGATCCGTAGCGGTTATCACGGGGATACTTGGCACGCCATGTCGGTATGCGATCCGGTG
ACGGGTATGCATGGGATCTTCTCCGGAAGCCTACCCATCCAATACTTCATACCTCAACCTTCGGTCCGATTCGGCGAGGA
ATGGAGAGAGGAGGCCATGGCTCCCTTGCGGGATTTACTAGAGCGGCATGCCGACGAGATCGCCGCCTTGATCTTGGAAC
CGGTCGTACAGGGGGCAGGAGGCATGTATTTCTACTCCCCCACTTTCCTCGTTCGTGCCCGGGAACTTTGCGAGCGGTAT
AACGTCTTATTGATATTCGACGAGATAGCGACTGGCTTCGGGCGTACCGGAAAGTTGTTCGCATGGGAACATGCGGGAGT
CGAACCCGATATCATGTGTATCGGAAAGGCATTGACGGGAGGTTATCTTACGCTTTCCGCCACGATCACGACAAAGCCGA
TAGCCGACACGATCTGTTCCGGCGAGGCGGGATGCTTTATGCATGGACCTACGTTTATGGGAAATCCCTTGGCATGCGCT
ATCGCTTCGGCCTCGATCTCTTTATTGATCGCCAGTCACTGGCAGGAGAAAGTGAAGCTTATAGAGGAGCAATTGCGGGC
GGAGTTAGCGCCAGCCGCCAAGCTGCCTGAAGTCGCTGAGGTACGGGTATTGGGGGCGATCGGGGTTATCGAGATGAAGC
ATCCGGTGGATATGGCGGTATTGCAACGTCGATTCGTAGAGGAAGGGATATGGGTACGTCCATTCGGACGGCTGGTTTAT
CTCATGCCTCCTTTCGTTATCTCCACGTATGAACTATCCAGACTAACCTCCGGATTATTGAGAGTATTAACGCATAATTA
A

Upstream 100 bases:

>100_bases
TCGGAGACTTATTAACGACTATCGGAAGCAAGGTAGAGGAAGACAAAGTCTTATTTACCTCCGAAGGCTATTCACTCACC
GAAAACACCGATTGGGAAAA

Downstream 100 bases:

>100_bases
CATATCTACAACCGGGTAATATATAGATATATGAAATCATATAACGAGCAACTTACGGCTTTACGAGCCTCCGGGAATCT
TAGGCACCTACCGGAAGTCG

Product: adenosylmethionine--8-amino-7-oxononanoate transaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTTTELLQYDRDHLWHPYTSTIDPLPVYPVSKAEGVTITLADGRELIDGMSSWWAAVHGYNHPVLNAAAKAQLDKMSHIM
FGGFTHEPAVELTAKLLPLLPPSLEKIFLADSGSVAVEVALKMAIQYWQSKGIPGKHTFATIRSGYHGDTWHAMSVCDPV
TGMHGIFSGSLPIQYFIPQPSVRFGEEWREEAMAPLRDLLERHADEIAALILEPVVQGAGGMYFYSPTFLVRARELCERY
NVLLIFDEIATGFGRTGKLFAWEHAGVEPDIMCIGKALTGGYLTLSATITTKPIADTICSGEAGCFMHGPTFMGNPLACA
IASASISLLIASHWQEKVKLIEEQLRAELAPAAKLPEVAEVRVLGAIGVIEMKHPVDMAVLQRRFVEEGIWVRPFGRLVY
LMPPFVISTYELSRLTSGLLRVLTHN

Sequences:

>Translated_426_residues
MTTTELLQYDRDHLWHPYTSTIDPLPVYPVSKAEGVTITLADGRELIDGMSSWWAAVHGYNHPVLNAAAKAQLDKMSHIM
FGGFTHEPAVELTAKLLPLLPPSLEKIFLADSGSVAVEVALKMAIQYWQSKGIPGKHTFATIRSGYHGDTWHAMSVCDPV
TGMHGIFSGSLPIQYFIPQPSVRFGEEWREEAMAPLRDLLERHADEIAALILEPVVQGAGGMYFYSPTFLVRARELCERY
NVLLIFDEIATGFGRTGKLFAWEHAGVEPDIMCIGKALTGGYLTLSATITTKPIADTICSGEAGCFMHGPTFMGNPLACA
IASASISLLIASHWQEKVKLIEEQLRAELAPAAKLPEVAEVRVLGAIGVIEMKHPVDMAVLQRRFVEEGIWVRPFGRLVY
LMPPFVISTYELSRLTSGLLRVLTHN
>Mature_425_residues
TTTELLQYDRDHLWHPYTSTIDPLPVYPVSKAEGVTITLADGRELIDGMSSWWAAVHGYNHPVLNAAAKAQLDKMSHIMF
GGFTHEPAVELTAKLLPLLPPSLEKIFLADSGSVAVEVALKMAIQYWQSKGIPGKHTFATIRSGYHGDTWHAMSVCDPVT
GMHGIFSGSLPIQYFIPQPSVRFGEEWREEAMAPLRDLLERHADEIAALILEPVVQGAGGMYFYSPTFLVRARELCERYN
VLLIFDEIATGFGRTGKLFAWEHAGVEPDIMCIGKALTGGYLTLSATITTKPIADTICSGEAGCFMHGPTFMGNPLACAI
ASASISLLIASHWQEKVKLIEEQLRAELAPAAKLPEVAEVRVLGAIGVIEMKHPVDMAVLQRRFVEEGIWVRPFGRLVYL
MPPFVISTYELSRLTSGLLRVLTHN

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=425, Percent_Identity=31.2941176470588, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI13994255, Length=383, Percent_Identity=29.7650130548303, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI284507298, Length=227, Percent_Identity=37.4449339207048, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI37574042, Length=410, Percent_Identity=24.390243902439, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI226442709, Length=393, Percent_Identity=24.6819338422392, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI226442705, Length=330, Percent_Identity=25.7575757575758, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786991, Length=421, Percent_Identity=61.2826603325416, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI145693181, Length=336, Percent_Identity=33.9285714285714, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1789016, Length=355, Percent_Identity=32.112676056338, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789759, Length=359, Percent_Identity=32.3119777158774, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1788044, Length=404, Percent_Identity=31.1881188118812, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787560, Length=397, Percent_Identity=31.2342569269521, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1786349, Length=328, Percent_Identity=29.5731707317073, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=430, Percent_Identity=26.046511627907, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25144274, Length=222, Percent_Identity=31.981981981982, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32564660, Length=370, Percent_Identity=27.027027027027, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71992977, Length=402, Percent_Identity=24.1293532338308, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17541228, Length=298, Percent_Identity=26.510067114094, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324386, Length=459, Percent_Identity=45.5337690631808, Blast_Score=415, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6323470, Length=435, Percent_Identity=27.3563218390805, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324432, Length=379, Percent_Identity=27.7044854881267, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321456, Length=331, Percent_Identity=25.3776435045317, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21357415, Length=415, Percent_Identity=30.8433734939759, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI28574759, Length=321, Percent_Identity=25.8566978193146, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161085790, Length=321, Percent_Identity=25.8566978193146, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356575, Length=390, Percent_Identity=24.1025641025641, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281366494, Length=362, Percent_Identity=26.7955801104972, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24667139, Length=362, Percent_Identity=26.7955801104972, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24667143, Length=362, Percent_Identity=26.7955801104972, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 46955; Mature: 46824

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTELLQYDRDHLWHPYTSTIDPLPVYPVSKAEGVTITLADGRELIDGMSSWWAAVHGY
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCC
NHPVLNAAAKAQLDKMSHIMFGGFTHEPAVELTAKLLPLLPPSLEKIFLADSGSVAVEVA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHH
LKMAIQYWQSKGIPGKHTFATIRSGYHGDTWHAMSVCDPVTGMHGIFSGSLPIQYFIPQP
HHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEHHHHHCCCCCHHHHCCCCCCEEEECCCC
SVRFGEEWREEAMAPLRDLLERHADEIAALILEPVVQGAGGMYFYSPTFLVRARELCERY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHC
NVLLIFDEIATGFGRTGKLFAWEHAGVEPDIMCIGKALTGGYLTLSATITTKPIADTICS
CEEEEEHHHHHCCCCCCCEEEEECCCCCCCCEEEEHHCCCCEEEEEEEEECCHHHHHHCC
GEAGCFMHGPTFMGNPLACAIASASISLLIASHWQEKVKLIEEQLRAELAPAAKLPEVAE
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VRVLGAIGVIEMKHPVDMAVLQRRFVEEGIWVRPFGRLVYLMPPFVISTYELSRLTSGLL
EEEEEEECEEEECCCHHHHHHHHHHHHCCCEECCCCCEEEECCCHHHHHHHHHHHHHHHH
RVLTHN
HHHCCC
>Mature Secondary Structure 
TTTELLQYDRDHLWHPYTSTIDPLPVYPVSKAEGVTITLADGRELIDGMSSWWAAVHGY
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCC
NHPVLNAAAKAQLDKMSHIMFGGFTHEPAVELTAKLLPLLPPSLEKIFLADSGSVAVEVA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHH
LKMAIQYWQSKGIPGKHTFATIRSGYHGDTWHAMSVCDPVTGMHGIFSGSLPIQYFIPQP
HHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEHHHHHCCCCCHHHHCCCCCCEEEECCCC
SVRFGEEWREEAMAPLRDLLERHADEIAALILEPVVQGAGGMYFYSPTFLVRARELCERY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHC
NVLLIFDEIATGFGRTGKLFAWEHAGVEPDIMCIGKALTGGYLTLSATITTKPIADTICS
CEEEEEHHHHHCCCCCCCEEEEECCCCCCCCEEEEHHCCCCEEEEEEEEECCHHHHHHCC
GEAGCFMHGPTFMGNPLACAIASASISLLIASHWQEKVKLIEEQLRAELAPAAKLPEVAE
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
VRVLGAIGVIEMKHPVDMAVLQRRFVEEGIWVRPFGRLVYLMPPFVISTYELSRLTSGLL
EEEEEEECEEEECCCHHHHHHHHHHHHCCCEECCCCCEEEECCCHHHHHHHHHHHHHHHH
RVLTHN
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA