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The map label for this gene is bioB

Identifier: 150008527

GI number: 150008527

Start: 2272443

End: 2273426

Strand: Direct

Name: bioB

Synonym: BDI_1913

Alternate gene names: 150008527

Gene position: 2272443-2273426 (Clockwise)

Preceding gene: 150008526

Following gene: 150008528

Centisome position: 47.23

GC content: 47.97

Gene sequence:

>984_bases
ATGACAATAGAAGAAGCAAAGCAGCACATCCTAGAGGGTGGGAAGATTACTGAGGAACAGGCTTTAAGCTTGGCGAATCA
CCCGGACAAAGAGGCGCTTTACGAGGCCGCCCACCAAATCACCCGCCATTTTATGGGGAATAAGTTCGACACTTGTTCCA
TCATCAACGCCAAAAGCGGTAATTGCAGCGAGGATTGTAAATGGTGCGCACAATCGGGACATTACAAGACCCTCGTAAAC
TTATATCCTTTGCTTCCGGCCAAGGAATGTGTCTATCATGCGGTATATAACCGGAAACAGGGTATCCGGCGTTTTGCCTT
GGTGACTAGCGGTAAGCGAGTATCGGATAAAGAATTGGAACAGATCACCGATACGATCCGGCAAATCAAGCGGCAAAGCG
ATATTAAATGCTGTGCCTCCATGGGATTGCTAACCCGCTCCCAACTCCAATCGCTTTACGATAGCGGAGTGGAGAATTAT
CACTGCAATATCGAGACTGCCCCTTCCTATTTCCGTCAACTCTGTTCCACGCATACGATTGAGCAGAAGATGGAAACCAT
CCATACCGCCCGGGAAATCGGTTTCCGCATCTGCTGCGGTGGTATTATCGGCATGGGCGAGACGATGAAGGAGCGAATCG
AGATGGCTTGTTTCTTACAGAAAGAAGGTGTCTTATCCATCCCTCTCAATTTATTACAGCCGATACCGGGAACTCCTATG
GAGAATACACAGATCCTAGAAGAAGAGGAATGGCTAACAACGATCGCCCTTTTCCGCTTGATCAATCCAAACGCATTTCT
CCGTTTCTCCGGCGGACGGGCACAATTAAGCGAGGTCACGCAACGGAAATCCCTACATATAGGGATTAACTCCGCGATCA
TCGGAGACTTATTAACGACTATCGGAAGCAAGGTAGAGGAAGACAAAGTCTTATTTACCTCCGAAGGCTATTCACTCACC
GAAAACACCGATTGGGAAAAATGA

Upstream 100 bases:

>100_bases
TACTTTCCGCCTTTCTTATTTCCATCCGGTTGTAGAGACGGGACGTGCCCCGTCTCTACAACTGGGTATATATATACACG
GAATTATTAAATGAGAGAAA

Downstream 100 bases:

>100_bases
CAACAACCGAACTTTTACAATACGACCGGGATCATCTATGGCATCCCTACACCTCGACCATCGATCCACTTCCCGTCTAT
CCGGTATCCAAAGCGGAGGG

Product: biotin synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTIEEAKQHILEGGKITEEQALSLANHPDKEALYEAAHQITRHFMGNKFDTCSIINAKSGNCSEDCKWCAQSGHYKTLVN
LYPLLPAKECVYHAVYNRKQGIRRFALVTSGKRVSDKELEQITDTIRQIKRQSDIKCCASMGLLTRSQLQSLYDSGVENY
HCNIETAPSYFRQLCSTHTIEQKMETIHTAREIGFRICCGGIIGMGETMKERIEMACFLQKEGVLSIPLNLLQPIPGTPM
ENTQILEEEEWLTTIALFRLINPNAFLRFSGGRAQLSEVTQRKSLHIGINSAIIGDLLTTIGSKVEEDKVLFTSEGYSLT
ENTDWEK

Sequences:

>Translated_327_residues
MTIEEAKQHILEGGKITEEQALSLANHPDKEALYEAAHQITRHFMGNKFDTCSIINAKSGNCSEDCKWCAQSGHYKTLVN
LYPLLPAKECVYHAVYNRKQGIRRFALVTSGKRVSDKELEQITDTIRQIKRQSDIKCCASMGLLTRSQLQSLYDSGVENY
HCNIETAPSYFRQLCSTHTIEQKMETIHTAREIGFRICCGGIIGMGETMKERIEMACFLQKEGVLSIPLNLLQPIPGTPM
ENTQILEEEEWLTTIALFRLINPNAFLRFSGGRAQLSEVTQRKSLHIGINSAIIGDLLTTIGSKVEEDKVLFTSEGYSLT
ENTDWEK
>Mature_326_residues
TIEEAKQHILEGGKITEEQALSLANHPDKEALYEAAHQITRHFMGNKFDTCSIINAKSGNCSEDCKWCAQSGHYKTLVNL
YPLLPAKECVYHAVYNRKQGIRRFALVTSGKRVSDKELEQITDTIRQIKRQSDIKCCASMGLLTRSQLQSLYDSGVENYH
CNIETAPSYFRQLCSTHTIEQKMETIHTAREIGFRICCGGIIGMGETMKERIEMACFLQKEGVLSIPLNLLQPIPGTPME
NTQILEEEEWLTTIALFRLINPNAFLRFSGGRAQLSEVTQRKSLHIGINSAIIGDLLTTIGSKVEEDKVLFTSEGYSLTE
NTDWEK

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=29.5081967213115, Blast_Score=169, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321725, Length=325, Percent_Identity=28, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_PARD8 (A6LD84)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303270.1
- ProteinModelPortal:   A6LD84
- SMR:   A6LD84
- STRING:   A6LD84
- GeneID:   5307063
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_1913
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   TPLMELV
- ProtClustDB:   CLSK826901
- BioCyc:   PDIS435591:BDI_1913-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36872; Mature: 36740

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEEAKQHILEGGKITEEQALSLANHPDKEALYEAAHQITRHFMGNKFDTCSIINAKSG
CCHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
NCSEDCKWCAQSGHYKTLVNLYPLLPAKECVYHAVYNRKQGIRRFALVTSGKRVSDKELE
CCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QITDTIRQIKRQSDIKCCASMGLLTRSQLQSLYDSGVENYHCNIETAPSYFRQLCSTHTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHH
EQKMETIHTAREIGFRICCGGIIGMGETMKERIEMACFLQKEGVLSIPLNLLQPIPGTPM
HHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHCCCCEECCHHHHCCCCCCCC
ENTQILEEEEWLTTIALFRLINPNAFLRFSGGRAQLSEVTQRKSLHIGINSAIIGDLLTT
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHEEECCHHHHHHHHHHH
IGSKVEEDKVLFTSEGYSLTENTDWEK
HCCCCCCCCEEEEECCCEECCCCCCCC
>Mature Secondary Structure 
TIEEAKQHILEGGKITEEQALSLANHPDKEALYEAAHQITRHFMGNKFDTCSIINAKSG
CHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
NCSEDCKWCAQSGHYKTLVNLYPLLPAKECVYHAVYNRKQGIRRFALVTSGKRVSDKELE
CCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QITDTIRQIKRQSDIKCCASMGLLTRSQLQSLYDSGVENYHCNIETAPSYFRQLCSTHTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHH
EQKMETIHTAREIGFRICCGGIIGMGETMKERIEMACFLQKEGVLSIPLNLLQPIPGTPM
HHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHCCCCEECCHHHHCCCCCCCC
ENTQILEEEEWLTTIALFRLINPNAFLRFSGGRAQLSEVTQRKSLHIGINSAIIGDLLTT
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHEEECCHHHHHHHHHHH
IGSKVEEDKVLFTSEGYSLTENTDWEK
HCCCCCCCCEEEEECCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA