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The map label for this gene is potC [H]

Identifier: 150008277

GI number: 150008277

Start: 1943379

End: 1944167

Strand: Reverse

Name: potC [H]

Synonym: BDI_1646

Alternate gene names: 150008277

Gene position: 1944167-1943379 (Counterclockwise)

Preceding gene: 150008278

Following gene: 150008276

Centisome position: 40.41

GC content: 48.8

Gene sequence:

>789_bases
ATGGTGAAATATTTAGCTAAGGGATATCTGTGGGCTTTGCTTTTGTTGCTCTACTCGCCGATCCTGATTATCATGATCTT
CTCGTTCACGGAGGCGAAAGTGCTGGGTAACTGGACCGGGTTCTCCACGAAATTATATAGCTCCCTTTTTGCCGGCAACG
TGCAGCACTCGTTGGTAAGCGCCCTTTGGAATACGTTCGCTATCGCCATGATAGCCGCTACGGTATCTACCTTGTTGGGA
AGCGTGGCCGCTATCGGGATCTTTAATTTGAAGACAAGGGCCCGTAACGCCGTGAGCTTTGTCAATAACATCCCGATGTT
GAATCCGGATATCATCACCGGTATTTCCTTGTTCCTGTTGTTTGTCAGCTTAGGGATCTCTCAAGGTTTGACGACGGTCG
TGTTGGCGCATATCACGTTCTGTACGCCGTATGTGGTATTAAGCGTCATGCCCCGCTTGAAACAGATGAACCAGAATATC
TACGAGGCGGCGTTGGATTTGGGGGCGACTCCGTTTCAAGCCTTGCGGAAGGTGATCTTCCCGGAAATCCGTCCGGGTAT
GATCAGTGGTTTTATCCTTGCCTTCACGCTCTCTATCGACGATTTCGCCGTGACCATATTTACGATTGGTAATGAGGGCT
TGGAAACCCTTTCCACCTTTATCTATGCGGATGCCCGGAAAGGAGGATTGACCCCGGAGTTGCGTCCCCTCTCAACGATT
ATATTCGTAACCGTGTTAGTATTGTTGATTGTTATAAATAAACGTGCTGAGAAAGCGAAGCGAGCGTAA

Upstream 100 bases:

>100_bases
ACGGGGCGGCGCTTTCTCTTATCATGTTGTTATTAATCGGAATCACGGGATTGTTCAGTAATGAGAGCAGTGATTCGGCG
AATGAGGGAGGGCTTATCTG

Downstream 100 bases:

>100_bases
ATGAAAATGAAGAAATTTATATCGATTTTGTCTGTTTTGGCCCTGCTTATGGGGCTGTTCACTTCTTGCGGGCGCTCGGA
CGAGGAGAGAAGTAAGATCT

Product: putrescine transport system permease PotI

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MVKYLAKGYLWALLLLLYSPILIIMIFSFTEAKVLGNWTGFSTKLYSSLFAGNVQHSLVSALWNTFAIAMIAATVSTLLG
SVAAIGIFNLKTRARNAVSFVNNIPMLNPDIITGISLFLLFVSLGISQGLTTVVLAHITFCTPYVVLSVMPRLKQMNQNI
YEAALDLGATPFQALRKVIFPEIRPGMISGFILAFTLSIDDFAVTIFTIGNEGLETLSTFIYADARKGGLTPELRPLSTI
IFVTVLVLLIVINKRAEKAKRA

Sequences:

>Translated_262_residues
MVKYLAKGYLWALLLLLYSPILIIMIFSFTEAKVLGNWTGFSTKLYSSLFAGNVQHSLVSALWNTFAIAMIAATVSTLLG
SVAAIGIFNLKTRARNAVSFVNNIPMLNPDIITGISLFLLFVSLGISQGLTTVVLAHITFCTPYVVLSVMPRLKQMNQNI
YEAALDLGATPFQALRKVIFPEIRPGMISGFILAFTLSIDDFAVTIFTIGNEGLETLSTFIYADARKGGLTPELRPLSTI
IFVTVLVLLIVINKRAEKAKRA
>Mature_262_residues
MVKYLAKGYLWALLLLLYSPILIIMIFSFTEAKVLGNWTGFSTKLYSSLFAGNVQHSLVSALWNTFAIAMIAATVSTLLG
SVAAIGIFNLKTRARNAVSFVNNIPMLNPDIITGISLFLLFVSLGISQGLTTVVLAHITFCTPYVVLSVMPRLKQMNQNI
YEAALDLGATPFQALRKVIFPEIRPGMISGFILAFTLSIDDFAVTIFTIGNEGLETLSTFIYADARKGGLTPELRPLSTI
IFVTVLVLLIVINKRAEKAKRA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=223, Percent_Identity=40.3587443946188, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1787081, Length=254, Percent_Identity=36.6141732283465, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787714, Length=204, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788764, Length=74, Percent_Identity=40.5405405405405, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28659; Mature: 28659

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKYLAKGYLWALLLLLYSPILIIMIFSFTEAKVLGNWTGFSTKLYSSLFAGNVQHSLVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHH
ALWNTFAIAMIAATVSTLLGSVAAIGIFNLKTRARNAVSFVNNIPMLNPDIITGISLFLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FVSLGISQGLTTVVLAHITFCTPYVVLSVMPRLKQMNQNIYEAALDLGATPFQALRKVIF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
PEIRPGMISGFILAFTLSIDDFAVTIFTIGNEGLETLSTFIYADARKGGLTPELRPLSTI
CCCCCHHHHHHHHHHHEECCHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IFVTVLVLLIVINKRAEKAKRA
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKYLAKGYLWALLLLLYSPILIIMIFSFTEAKVLGNWTGFSTKLYSSLFAGNVQHSLVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHH
ALWNTFAIAMIAATVSTLLGSVAAIGIFNLKTRARNAVSFVNNIPMLNPDIITGISLFLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FVSLGISQGLTTVVLAHITFCTPYVVLSVMPRLKQMNQNIYEAALDLGATPFQALRKVIF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
PEIRPGMISGFILAFTLSIDDFAVTIFTIGNEGLETLSTFIYADARKGGLTPELRPLSTI
CCCCCHHHHHHHHHHHEECCHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IFVTVLVLLIVINKRAEKAKRA
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]