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The map label for this gene is potH [H]

Identifier: 150008278

GI number: 150008278

Start: 1944167

End: 1944964

Strand: Reverse

Name: potH [H]

Synonym: BDI_1647

Alternate gene names: 150008278

Gene position: 1944964-1944167 (Counterclockwise)

Preceding gene: 150008279

Following gene: 150008277

Centisome position: 40.42

GC content: 47.24

Gene sequence:

>798_bases
GTGATCAATACGTTTTCTGCATTTTTTATGAGACGAAGGAACTGGACCATTCCTTATGTCCTGTTCTTGGCCATCTTTGT
CGTGATACCGCTCTTCTTGATCGTGTATTACGCCTTTACGAATGACGAGGGGATGTTTACCTTCGCCAACTTCCGTAAGT
TCATGATCCATCCCGAGGCGATCAACACCTTCGTGTATTCAGTCGGGATCGCTTTGATAACGACCATCCTTTGTCTGCTG
CTGGGATATCCGGCCGCTTGGATATTGAGCCAAGCCCGGTTTAATACTTCCCGTACGATGGTCGTTTTGTTTATCCTTCC
GATGTGGGTCAATATCTTGATCCGTACGTTGGCTACCGTGGCTTTGTTCGATTTCTTGAATTTCCCGCTGGGAGAGGGGG
CCTTGATCTTTGGCATGGTGTACAACTTCTTGCCCTTTATGATCTACCCGATTTATAATACGCTCCAGAAAATGGATCAC
AGCTTGATAGAGGCGGCGCAAGACTTGGGGGCGAATCCGTCGCAGGTATTTATCAAGGCCGTATTCCCACTATCGATGCC
GGGAGTGATGAGTGGTATCATGATGGTGTTTATGCCGACCATCTCTACGTTTGCCATCGCCGAATTGTTGACGATGAACA
ATATCAAGCTCTTCGGTACGACCATACAGGAGAATATCAATAACGGTATGTGGAACTACGGGGCGGCGCTTTCTCTTATC
ATGTTGTTATTAATCGGAATCACGGGATTGTTCAGTAATGAGAGCAGTGATTCGGCGAATGAGGGAGGGCTTATCTGA

Upstream 100 bases:

>100_bases
CTTTGTCGATACCAACGATGTATGGGATGATGGAGATCATGTCGGCATAACGATCGCCCCGGATAAAATTCGAATTATTC
ATGCTTAAAAAGGGAAGGAA

Downstream 100 bases:

>100_bases
TGGTGAAATATTTAGCTAAGGGATATCTGTGGGCTTTGCTTTTGTTGCTCTACTCGCCGATCCTGATTATCATGATCTTC
TCGTTCACGGAGGCGAAAGT

Product: putrescine transport system permease PotH

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MINTFSAFFMRRRNWTIPYVLFLAIFVVIPLFLIVYYAFTNDEGMFTFANFRKFMIHPEAINTFVYSVGIALITTILCLL
LGYPAAWILSQARFNTSRTMVVLFILPMWVNILIRTLATVALFDFLNFPLGEGALIFGMVYNFLPFMIYPIYNTLQKMDH
SLIEAAQDLGANPSQVFIKAVFPLSMPGVMSGIMMVFMPTISTFAIAELLTMNNIKLFGTTIQENINNGMWNYGAALSLI
MLLLIGITGLFSNESSDSANEGGLI

Sequences:

>Translated_265_residues
MINTFSAFFMRRRNWTIPYVLFLAIFVVIPLFLIVYYAFTNDEGMFTFANFRKFMIHPEAINTFVYSVGIALITTILCLL
LGYPAAWILSQARFNTSRTMVVLFILPMWVNILIRTLATVALFDFLNFPLGEGALIFGMVYNFLPFMIYPIYNTLQKMDH
SLIEAAQDLGANPSQVFIKAVFPLSMPGVMSGIMMVFMPTISTFAIAELLTMNNIKLFGTTIQENINNGMWNYGAALSLI
MLLLIGITGLFSNESSDSANEGGLI
>Mature_265_residues
MINTFSAFFMRRRNWTIPYVLFLAIFVVIPLFLIVYYAFTNDEGMFTFANFRKFMIHPEAINTFVYSVGIALITTILCLL
LGYPAAWILSQARFNTSRTMVVLFILPMWVNILIRTLATVALFDFLNFPLGEGALIFGMVYNFLPFMIYPIYNTLQKMDH
SLIEAAQDLGANPSQVFIKAVFPLSMPGVMSGIMMVFMPTISTFAIAELLTMNNIKLFGTTIQENINNGMWNYGAALSLI
MLLLIGITGLFSNESSDSANEGGLI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=264, Percent_Identity=32.1969696969697, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787080, Length=235, Percent_Identity=34.468085106383, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787713, Length=197, Percent_Identity=27.9187817258883, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787368, Length=179, Percent_Identity=25.1396648044693, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29802; Mature: 29802

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.4 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINTFSAFFMRRRNWTIPYVLFLAIFVVIPLFLIVYYAFTNDEGMFTFANFRKFMIHPEA
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCHHH
INTFVYSVGIALITTILCLLLGYPAAWILSQARFNTSRTMVVLFILPMWVNILIRTLATV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALFDFLNFPLGEGALIFGMVYNFLPFMIYPIYNTLQKMDHSLIEAAQDLGANPSQVFIKA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VFPLSMPGVMSGIMMVFMPTISTFAIAELLTMNNIKLFGTTIQENINNGMWNYGAALSLI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHH
MLLLIGITGLFSNESSDSANEGGLI
HHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MINTFSAFFMRRRNWTIPYVLFLAIFVVIPLFLIVYYAFTNDEGMFTFANFRKFMIHPEA
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCHHH
INTFVYSVGIALITTILCLLLGYPAAWILSQARFNTSRTMVVLFILPMWVNILIRTLATV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALFDFLNFPLGEGALIFGMVYNFLPFMIYPIYNTLQKMDHSLIEAAQDLGANPSQVFIKA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VFPLSMPGVMSGIMMVFMPTISTFAIAELLTMNNIKLFGTTIQENINNGMWNYGAALSLI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHH
MLLLIGITGLFSNESSDSANEGGLI
HHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]