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The map label for this gene is potD-A [H]

Identifier: 150008276

GI number: 150008276

Start: 1942035

End: 1943378

Strand: Reverse

Name: potD-A [H]

Synonym: BDI_1645

Alternate gene names: 150008276

Gene position: 1943378-1942035 (Counterclockwise)

Preceding gene: 150008277

Following gene: 150008275

Centisome position: 40.39

GC content: 48.88

Gene sequence:

>1344_bases
ATGAAAATGAAGAAATTTATATCGATTTTGTCTGTTTTGGCCCTGCTTATGGGGCTGTTCACTTCTTGCGGGCGCTCGGA
CGAGGAGAGAAGTAAGATCTTGAAGATTTATAACTGGGGAGATTATATCGACGAGGACGTCCTGACGGATTTCCCGAAAT
GGTATAAGGAACAGACGGGTGAGGAGGTCCGGATTATCTATCAGGTCTTTGATATCAATGAGATCATGTTGACCAAGATC
GAACGGGGGCATGAGGATTTCGACTTGATTTGCCCTTCGGAGTATATCCTTGAGCGCATGCTGCGGAAAAATCTCTTGCT
ACCGATCGACCGTAATTTCGGGAAGACACCGGATTATATCAACAATGTCTCTCCCTATATCCAAGAGGAACTGAATAAGT
TAAGTCAGCCGGGCCGTAAGACTACCGACTACGTGGTTCCTTATATGTGGGGAACCGCCGGCCTGTTGTATAATAAAGCC
GATGTCTCGAGGGACGAGGTAATGTCGTGGAAATGCCTTTGGGACCCTCGTTTCAGGAATAAGATCTTGATGAAGGATAG
TTATCGTGACGCTTATGGAACCGCTATCATATACGCCCATGCGAGGGAATTGGCGAATGGTACGATCACGGTGGAGCAAT
TGATGAACGATAGCTCCCCGGAAATGATCGCTATCGCCGAGGAATACTTGAAGAAGATGAAACCGAATATCGCCGGTTGG
GAGGCCGATTTCGGTAAGGAGATGATGACGAAAGGAAAGGCGTGGCTGAATTTCACTTGGAGCGGCGACGCGGTCTGGGC
CATGGAAGAGGCGAGTGCCGTAGGCGTAGATCTGGGGTATGAGGTTCCTTTGGAGGGTAGTAATATCTGGTACGATGGTT
GGGCGATCCCTAAATACGCTCGCAACGTGAAGGCGGCCAGTTATTTTATGGACTATCTATGCCGTCCGGACATCGTCAGC
CGTAATATGGAGGTGACCGGATATGTCAGCACCGTGGCCACCCCGGAAATATTGGCGGAAAAGATCGATACGACGCAGAC
CGAGTTCTCGGACGTAAGTTATTTCTTCGGACCGGGAGCGGAGCGTGTCCAGATAGACCCGGCTCAATATCCGGACCGGA
AGGTCGTGGAACGTTGTGCCATGATCCGTGATTTCGGGAACGAGACGGAGGTGGTTCTGGAAATGTGGAGCCGGGTAAAA
GGTGACAATCTTAACTCTTGGATTGTTATATTGATATTCGCTGTTTTCGGCTTGTTGTTTGTCTGGGTCGTTTACAAGCG
GATCAATCGTTATCAACAGAAAAGACGACATCGCAGACGTCGTAGCTGGTATAAAAAGAAATGA

Upstream 100 bases:

>100_bases
GACCCCGGAGTTGCGTCCCCTCTCAACGATTATATTCGTAACCGTGTTAGTATTGTTGATTGTTATAAATAAACGTGCTG
AGAAAGCGAAGCGAGCGTAA

Downstream 100 bases:

>100_bases
TATGGAAGGCATAAAGAACTTGATTATAGACTTGGGCGGTGTGCTTATTAACCTGACTCGCAACCGTTGTATCGAGGCTT
TCGAGAATCTGGGCGTGGAG

Product: spermidine/putrescine ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKMKKFISILSVLALLMGLFTSCGRSDEERSKILKIYNWGDYIDEDVLTDFPKWYKEQTGEEVRIIYQVFDINEIMLTKI
ERGHEDFDLICPSEYILERMLRKNLLLPIDRNFGKTPDYINNVSPYIQEELNKLSQPGRKTTDYVVPYMWGTAGLLYNKA
DVSRDEVMSWKCLWDPRFRNKILMKDSYRDAYGTAIIYAHARELANGTITVEQLMNDSSPEMIAIAEEYLKKMKPNIAGW
EADFGKEMMTKGKAWLNFTWSGDAVWAMEEASAVGVDLGYEVPLEGSNIWYDGWAIPKYARNVKAASYFMDYLCRPDIVS
RNMEVTGYVSTVATPEILAEKIDTTQTEFSDVSYFFGPGAERVQIDPAQYPDRKVVERCAMIRDFGNETEVVLEMWSRVK
GDNLNSWIVILIFAVFGLLFVWVVYKRINRYQQKRRHRRRRSWYKKK

Sequences:

>Translated_447_residues
MKMKKFISILSVLALLMGLFTSCGRSDEERSKILKIYNWGDYIDEDVLTDFPKWYKEQTGEEVRIIYQVFDINEIMLTKI
ERGHEDFDLICPSEYILERMLRKNLLLPIDRNFGKTPDYINNVSPYIQEELNKLSQPGRKTTDYVVPYMWGTAGLLYNKA
DVSRDEVMSWKCLWDPRFRNKILMKDSYRDAYGTAIIYAHARELANGTITVEQLMNDSSPEMIAIAEEYLKKMKPNIAGW
EADFGKEMMTKGKAWLNFTWSGDAVWAMEEASAVGVDLGYEVPLEGSNIWYDGWAIPKYARNVKAASYFMDYLCRPDIVS
RNMEVTGYVSTVATPEILAEKIDTTQTEFSDVSYFFGPGAERVQIDPAQYPDRKVVERCAMIRDFGNETEVVLEMWSRVK
GDNLNSWIVILIFAVFGLLFVWVVYKRINRYQQKRRHRRRRSWYKKK
>Mature_447_residues
MKMKKFISILSVLALLMGLFTSCGRSDEERSKILKIYNWGDYIDEDVLTDFPKWYKEQTGEEVRIIYQVFDINEIMLTKI
ERGHEDFDLICPSEYILERMLRKNLLLPIDRNFGKTPDYINNVSPYIQEELNKLSQPGRKTTDYVVPYMWGTAGLLYNKA
DVSRDEVMSWKCLWDPRFRNKILMKDSYRDAYGTAIIYAHARELANGTITVEQLMNDSSPEMIAIAEEYLKKMKPNIAGW
EADFGKEMMTKGKAWLNFTWSGDAVWAMEEASAVGVDLGYEVPLEGSNIWYDGWAIPKYARNVKAASYFMDYLCRPDIVS
RNMEVTGYVSTVATPEILAEKIDTTQTEFSDVSYFFGPGAERVQIDPAQYPDRKVVERCAMIRDFGNETEVVLEMWSRVK
GDNLNSWIVILIFAVFGLLFVWVVYKRINRYQQKRRHRRRRSWYKKK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=381, Percent_Identity=26.509186351706, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1787078, Length=351, Percent_Identity=29.9145299145299, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 52254; Mature: 52254

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKKFISILSVLALLMGLFTSCGRSDEERSKILKIYNWGDYIDEDVLTDFPKWYKEQTG
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHCCC
EEVRIIYQVFDINEIMLTKIERGHEDFDLICPSEYILERMLRKNLLLPIDRNFGKTPDYI
CCEEEEEEHHCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCCCCEECCCCCCCCHHH
NNVSPYIQEELNKLSQPGRKTTDYVVPYMWGTAGLLYNKADVSRDEVMSWKCLWDPRFRN
HCCCHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHEECCCCCCHHHHCEEEEEECHHHCC
KILMKDSYRDAYGTAIIYAHARELANGTITVEQLMNDSSPEMIAIAEEYLKKMKPNIAGW
EEEEECCCCHHHHHEEEEEHHHHHCCCCEEHHHHHCCCCCCEEHHHHHHHHHHCCCCCCC
EADFGKEMMTKGKAWLNFTWSGDAVWAMEEASAVGVDLGYEVPLEGSNIWYDGWAIPKYA
CHHHHHHHHHCCCEEEEEEECCCEEEEEHHCCCCCCCCCCCCCCCCCCEEECCCCCHHHH
RNVKAASYFMDYLCRPDIVSRNMEVTGYVSTVATPEILAEKIDTTQTEFSDVSYFFGPGA
HHHHHHHHHHHHHCCCCHHHCCCEEEEEHHHHCCHHHHHHHHCCCHHHHHCCHHEECCCC
ERVQIDPAQYPDRKVVERCAMIRDFGNETEVVLEMWSRVKGDNLNSWIVILIFAVFGLLF
CEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VWVVYKRINRYQQKRRHRRRRSWYKKK
HHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKMKKFISILSVLALLMGLFTSCGRSDEERSKILKIYNWGDYIDEDVLTDFPKWYKEQTG
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHCCC
EEVRIIYQVFDINEIMLTKIERGHEDFDLICPSEYILERMLRKNLLLPIDRNFGKTPDYI
CCEEEEEEHHCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCCCCEECCCCCCCCHHH
NNVSPYIQEELNKLSQPGRKTTDYVVPYMWGTAGLLYNKADVSRDEVMSWKCLWDPRFRN
HCCCHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHEECCCCCCHHHHCEEEEEECHHHCC
KILMKDSYRDAYGTAIIYAHARELANGTITVEQLMNDSSPEMIAIAEEYLKKMKPNIAGW
EEEEECCCCHHHHHEEEEEHHHHHCCCCEEHHHHHCCCCCCEEHHHHHHHHHHCCCCCCC
EADFGKEMMTKGKAWLNFTWSGDAVWAMEEASAVGVDLGYEVPLEGSNIWYDGWAIPKYA
CHHHHHHHHHCCCEEEEEEECCCEEEEEHHCCCCCCCCCCCCCCCCCCEEECCCCCHHHH
RNVKAASYFMDYLCRPDIVSRNMEVTGYVSTVATPEILAEKIDTTQTEFSDVSYFFGPGA
HHHHHHHHHHHHHCCCCHHHCCCEEEEEHHHHCCHHHHHHHHCCCHHHHHCCHHEECCCC
ERVQIDPAQYPDRKVVERCAMIRDFGNETEVVLEMWSRVKGDNLNSWIVILIFAVFGLLF
CEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VWVVYKRINRYQQKRRHRRRRSWYKKK
HHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]