Click here to switch to the map view.

The map label for this gene is trpA

Identifier: 150007241

GI number: 150007241

Start: 686715

End: 687488

Strand: Reverse

Name: trpA

Synonym: BDI_0586

Alternate gene names: 150007241

Gene position: 687488-686715 (Counterclockwise)

Preceding gene: 150007242

Following gene: 150007240

Centisome position: 14.29

GC content: 51.68

Gene sequence:

>774_bases
ATGAATCGCATCACAAACTTATTCCAGACCCAGAAAGACGGAATACTCTCCGTCTATTTCACCGCCGGTTATCCGAACTT
AAACGATACGGCAAGCATCTTAAAAGCCTTACAGGCTAAAGGTATCCACATGGTAGAAGTAGGAATCCCCTTCTCCGACC
CGATGGCAGACGGCCCTGTCATCCAAGAGGCCGCTACGCAAGCGTTGCGTAACGGGATGTCACTCCACCTCCTATTCGGG
CAATTGAGAGAGATCCGGAGCGAAGTACAGATCCCGATCATCCTGATGGGCTACCTGAATCCTATCATGCAATACGGTTT
CGAGAAATTCTGTGCCAGTTGCGTAGAGGCCGGCGTAGACGGAATGATCATCCCCGACCTGCCTTACGCCGATTATATCG
CCGACTACAAGGAGATCGCCGATCGCCATGACCTGAAAATGATCATGCTGATCACCCCAGAGACCTCCGAGGAACGAATC
CGGCTGATCGATGCCCATACCAGCGGATTTATCTATATGGTATCCAGCGCCGCCACGACCGGAGCCCAACAAGACTTCAA
CGAGCAGAAGCAGGCCTATTTCCGTCGTATCAACGCCATGAACCTACAGAATCCCCGCTTGGTAGGTTTCGGTATCAGCA
ATAAAGCCACTTTCGAGGCGGCGATAGCCCATTCTTCCGGTGCCATCATCGGCAGTAAGTTCGTACAGTTACTAAAGAGC
GAAGCTACTCCCGCCGAAGCGGTCGATAAGTTACTGGAAGCCCTAAAACAATAG

Upstream 100 bases:

>100_bases
AAACTAAAGAATTTCATCCAACAAATTTTACATCCAGCTGTAGAGACGGGGCGTGCCCCGTCTCCCACCGTCTGATAGTA
TTTTTAAAATAGAATTCAAA

Downstream 100 bases:

>100_bases
GAACCCTATTCATGTTTACATATAAAAACAGTAAATAGTCCTATGAATAACTTCACTTTTCACAACCCAACCCGTTTATT
ATTCGGTAAGGGCATGATTG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MNRITNLFQTQKDGILSVYFTAGYPNLNDTASILKALQAKGIHMVEVGIPFSDPMADGPVIQEAATQALRNGMSLHLLFG
QLREIRSEVQIPIILMGYLNPIMQYGFEKFCASCVEAGVDGMIIPDLPYADYIADYKEIADRHDLKMIMLITPETSEERI
RLIDAHTSGFIYMVSSAATTGAQQDFNEQKQAYFRRINAMNLQNPRLVGFGISNKATFEAAIAHSSGAIIGSKFVQLLKS
EATPAEAVDKLLEALKQ

Sequences:

>Translated_257_residues
MNRITNLFQTQKDGILSVYFTAGYPNLNDTASILKALQAKGIHMVEVGIPFSDPMADGPVIQEAATQALRNGMSLHLLFG
QLREIRSEVQIPIILMGYLNPIMQYGFEKFCASCVEAGVDGMIIPDLPYADYIADYKEIADRHDLKMIMLITPETSEERI
RLIDAHTSGFIYMVSSAATTGAQQDFNEQKQAYFRRINAMNLQNPRLVGFGISNKATFEAAIAHSSGAIIGSKFVQLLKS
EATPAEAVDKLLEALKQ
>Mature_257_residues
MNRITNLFQTQKDGILSVYFTAGYPNLNDTASILKALQAKGIHMVEVGIPFSDPMADGPVIQEAATQALRNGMSLHLLFG
QLREIRSEVQIPIILMGYLNPIMQYGFEKFCASCVEAGVDGMIIPDLPYADYIADYKEIADRHDLKMIMLITPETSEERI
RLIDAHTSGFIYMVSSAATTGAQQDFNEQKQAYFRRINAMNLQNPRLVGFGISNKATFEAAIAHSSGAIIGSKFVQLLKS
EATPAEAVDKLLEALKQ

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=217, Percent_Identity=32.7188940092166, Blast_Score=116, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321412, Length=223, Percent_Identity=40.3587443946188, Blast_Score=173, Evalue=3e-44,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_PARD8 (A6L9J8)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001301984.1
- ProteinModelPortal:   A6L9J8
- SMR:   A6L9J8
- STRING:   A6L9J8
- GeneID:   5305741
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_0586
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   VILMTYY
- ProtClustDB:   PRK13111
- BioCyc:   PDIS435591:BDI_0586-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28368; Mature: 28368

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 46-46 ACT_SITE 57-57

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRITNLFQTQKDGILSVYFTAGYPNLNDTASILKALQAKGIHMVEVGIPFSDPMADGPV
CCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHH
IQEAATQALRNGMSLHLLFGQLREIRSEVQIPIILMGYLNPIMQYGFEKFCASCVEAGVD
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GMIIPDLPYADYIADYKEIADRHDLKMIMLITPETSEERIRLIDAHTSGFIYMVSSAATT
CEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCC
GAQQDFNEQKQAYFRRINAMNLQNPRLVGFGISNKATFEAAIAHSSGAIIGSKFVQLLKS
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHC
EATPAEAVDKLLEALKQ
CCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNRITNLFQTQKDGILSVYFTAGYPNLNDTASILKALQAKGIHMVEVGIPFSDPMADGPV
CCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHH
IQEAATQALRNGMSLHLLFGQLREIRSEVQIPIILMGYLNPIMQYGFEKFCASCVEAGVD
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GMIIPDLPYADYIADYKEIADRHDLKMIMLITPETSEERIRLIDAHTSGFIYMVSSAATT
CEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCC
GAQQDFNEQKQAYFRRINAMNLQNPRLVGFGISNKATFEAAIAHSSGAIIGSKFVQLLKS
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHC
EATPAEAVDKLLEALKQ
CCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA