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The map label for this gene is ansA [H]

Identifier: 150006574

GI number: 150006574

Start: 5036308

End: 5037351

Strand: Direct

Name: ansA [H]

Synonym: BVU_4094

Alternate gene names: 150006574

Gene position: 5036308-5037351 (Clockwise)

Preceding gene: 150006573

Following gene: 150006575

Centisome position: 97.54

GC content: 46.07

Gene sequence:

>1044_bases
ATGATACAGAGTGAATATCCTTCCGTTTTGCTAATATACACAGGCGGTACGATCGGCATGATTGAAAATCCTGAGACAGG
TGCACTAGAAGCCTTTAATTTCGACCAGTTACAAGACAATGTCCCCGAATTGAAGCGGTTCAACTATCGCATCTCCTCTT
ACCAATTCAATCCCCCCATTGACTCTTCGGATATGGAACCTGCCTTGTGGGCCAAGTTAGTCAAGATCATCAAATACAAT
TATGATAATTTTGACGGGTTCGTCATTCTGCACGGTACAGACACCATGGCATACACCGCTTCAGCGCTCAGCTTCATGCT
GGAAAACCTGAGCAAGCCGGTTATCCTGACTGGCAGCCAGCTGCCCATCGGCATGTTGCGCACCGATGGAAAGGAAAATC
TGATCACTTCCATAGAAATAGCCGCTGCCAAACATCCGAACGGCACTGCTATTGTACCCGAAGTATGCATCTTCTTCGAA
AACCATCTGTTGCGCGGAAACCGTACCACAAAAATAAATGCGGAGAATTTCAATGCGTTCCGTTCATACAATTTCCCTTC
GCTGGCAACAGCAGGCATCCACATAAAATATGAATATGACCGTATTCACAAAGCCGATCCCAATCTGCCATTAAAACCAC
ACTATCTTTATGATACCAATGTAATTATACTGACCCTTTTTCCGGGCATCCAGGAAAACATGATCAGCAATATTCTGCAT
ACACCGGGATTACGCGCAGTGGTATTAAAGACCTACGGTTCGGGCAACGCTCCGCAAAAGCCGTGGTTCATCGAACTTCT
AAGGGATGCCACCTCACGGGGAATCATCATTGTCAACATCTCCCAATGCTCTACCGGAACAGTGGAAATGGGAAGGTATG
AAACCGGACTCCATCTGCTGGACGCAGGGGTCATCAGCGGCTATGACAGTACGGTGGAATCCGTTCTAACCAAACTGATG
TTCCTTTTGGGACACGGAAAAAGTGAAAGAGAAATACGTTACCAAATGAGTATTCCAGTAGCCGGAGAGTTTACGAAATC
ATAA

Upstream 100 bases:

>100_bases
ATCACCTATAAAGAAAAACATTTTGTTATTTCCCTCTCTTATTTTTGTCATACTTATACATAATTACTATCTTTGTGCCA
AAATTAAACAAGAGAAGCGC

Downstream 100 bases:

>100_bases
TTTATTGCGTTCCAATTATCATCCGGCGGGATGATTGCAGAATCATTAAAATGGTGTAATCTGCGATGAGAACTTAACGA
TTTTATAGCATGTAATCTTT

Product: L-asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MIQSEYPSVLLIYTGGTIGMIENPETGALEAFNFDQLQDNVPELKRFNYRISSYQFNPPIDSSDMEPALWAKLVKIIKYN
YDNFDGFVILHGTDTMAYTASALSFMLENLSKPVILTGSQLPIGMLRTDGKENLITSIEIAAAKHPNGTAIVPEVCIFFE
NHLLRGNRTTKINAENFNAFRSYNFPSLATAGIHIKYEYDRIHKADPNLPLKPHYLYDTNVIILTLFPGIQENMISNILH
TPGLRAVVLKTYGSGNAPQKPWFIELLRDATSRGIIIVNISQCSTGTVEMGRYETGLHLLDAGVISGYDSTVESVLTKLM
FLLGHGKSEREIRYQMSIPVAGEFTKS

Sequences:

>Translated_347_residues
MIQSEYPSVLLIYTGGTIGMIENPETGALEAFNFDQLQDNVPELKRFNYRISSYQFNPPIDSSDMEPALWAKLVKIIKYN
YDNFDGFVILHGTDTMAYTASALSFMLENLSKPVILTGSQLPIGMLRTDGKENLITSIEIAAAKHPNGTAIVPEVCIFFE
NHLLRGNRTTKINAENFNAFRSYNFPSLATAGIHIKYEYDRIHKADPNLPLKPHYLYDTNVIILTLFPGIQENMISNILH
TPGLRAVVLKTYGSGNAPQKPWFIELLRDATSRGIIIVNISQCSTGTVEMGRYETGLHLLDAGVISGYDSTVESVLTKLM
FLLGHGKSEREIRYQMSIPVAGEFTKS
>Mature_347_residues
MIQSEYPSVLLIYTGGTIGMIENPETGALEAFNFDQLQDNVPELKRFNYRISSYQFNPPIDSSDMEPALWAKLVKIIKYN
YDNFDGFVILHGTDTMAYTASALSFMLENLSKPVILTGSQLPIGMLRTDGKENLITSIEIAAAKHPNGTAIVPEVCIFFE
NHLLRGNRTTKINAENFNAFRSYNFPSLATAGIHIKYEYDRIHKADPNLPLKPHYLYDTNVIILTLFPGIQENMISNILH
TPGLRAVVLKTYGSGNAPQKPWFIELLRDATSRGIIIVNISQCSTGTVEMGRYETGLHLLDAGVISGYDSTVESVLTKLM
FLLGHGKSEREIRYQMSIPVAGEFTKS

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=360, Percent_Identity=41.3888888888889, Blast_Score=239, Evalue=2e-63,
Organism=Escherichia coli, GI1788065, Length=348, Percent_Identity=45.6896551724138, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI1789327, Length=255, Percent_Identity=27.4509803921569, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558278, Length=354, Percent_Identity=38.9830508474576, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17558280, Length=354, Percent_Identity=38.9830508474576, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI193207374, Length=354, Percent_Identity=38.9830508474576, Blast_Score=230, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI193207372, Length=354, Percent_Identity=38.9830508474576, Blast_Score=230, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6320527, Length=276, Percent_Identity=24.2753623188406, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323189, Length=304, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323187, Length=304, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323186, Length=304, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323184, Length=304, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24639609, Length=368, Percent_Identity=41.0326086956522, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24645530, Length=364, Percent_Identity=39.2857142857143, Blast_Score=234, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 38738; Mature: 38738

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSEYPSVLLIYTGGTIGMIENPETGALEAFNFDQLQDNVPELKRFNYRISSYQFNPPI
CCCCCCCCEEEEEECCEEEEEECCCCCCEEECCHHHHHCCCCHHHHHCEEEEEEEECCCC
DSSDMEPALWAKLVKIIKYNYDNFDGFVILHGTDTMAYTASALSFMLENLSKPVILTGSQ
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCC
LPIGMLRTDGKENLITSIEIAAAKHPNGTAIVPEVCIFFENHLLRGNRTTKINAENFNAF
CCEEEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHEECCCCEEEEECCCCCHH
RSYNFPSLATAGIHIKYEYDRIHKADPNLPLKPHYLYDTNVIILTLFPGIQENMISNILH
HCCCCCCEEEEEEEEEEEHHHHCCCCCCCCCCCEEEEECCEEEEEECCCHHHHHHHHHHC
TPGLRAVVLKTYGSGNAPQKPWFIELLRDATSRGIIIVNISQCSTGTVEMGRYETGLHLL
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEECCCCCCCCEEE
DAGVISGYDSTVESVLTKLMFLLGHGKSEREIRYQMSIPVAGEFTKS
EHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MIQSEYPSVLLIYTGGTIGMIENPETGALEAFNFDQLQDNVPELKRFNYRISSYQFNPPI
CCCCCCCCEEEEEECCEEEEEECCCCCCEEECCHHHHHCCCCHHHHHCEEEEEEEECCCC
DSSDMEPALWAKLVKIIKYNYDNFDGFVILHGTDTMAYTASALSFMLENLSKPVILTGSQ
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCC
LPIGMLRTDGKENLITSIEIAAAKHPNGTAIVPEVCIFFENHLLRGNRTTKINAENFNAF
CCEEEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHEECCCCEEEEECCCCCHH
RSYNFPSLATAGIHIKYEYDRIHKADPNLPLKPHYLYDTNVIILTLFPGIQENMISNILH
HCCCCCCEEEEEEEEEEEHHHHCCCCCCCCCCCEEEEECCEEEEEECCCHHHHHHHHHHC
TPGLRAVVLKTYGSGNAPQKPWFIELLRDATSRGIIIVNISQCSTGTVEMGRYETGLHLL
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEECCCCCCCCEEE
DAGVISGYDSTVESVLTKLMFLLGHGKSEREIRYQMSIPVAGEFTKS
EHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]