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The map label for this gene is trpA

Identifier: 150006573

GI number: 150006573

Start: 5035254

End: 5036030

Strand: Direct

Name: trpA

Synonym: BVU_4093

Alternate gene names: 150006573

Gene position: 5035254-5036030 (Clockwise)

Preceding gene: 150006572

Following gene: 150006574

Centisome position: 97.52

GC content: 46.33

Gene sequence:

>777_bases
ATGAACAGAATCAACCAGTTATTCAGTACCAAGCAAAAAGACATCCTATCTATTTATTTTTGTGCAGGCTTTCCTACCTT
AGAAGGTACGGCAAGCACCATCAAAGTCCTGGAAAAAAAAGGGATTAACATGATTGAAATCGGCATTCCTTTCAGTGACC
CGATGGCTGACGGTCCCGTTATCCAGCACGCCGCCACCCGCGCCTTGAAAAATGGCATGACACTGAAGCTCCTTTTCGAC
CAACTGAAAGATATCCGTAAAGAAGTACAGATTCCGCTGGTGCTGATGGGATATCTCAATCCCATCATGCAATATGGATT
CAAGGATTTCTGCCGCACTTGCCGGGAGACGGGAATCGACGGAGTGATTATCCCCGACTTGCCTTTCAAAGATTATATGG
AGGAATATCGTTCCATAGCCGAGGAACAAGATGTGAGAATCATCATGCTCATCACTCCGGAAACCAGTGAAGAACGTATC
CGCCTGATTGATGAACATACCGACGGTTTTATCTATATGGTATCTTCCGCCGCTATCACCGGTGCTCAAAAAGATTTCAA
CGCACAGAAACAAGCCTATTTCCAACGCATCGCCGACATGAATTTACGCAATCCCCGCATGATAGGTTTCGGAATTTCCA
ATAAGCAAACCTTCGAAACCGCATCCGCTCATGCCGCAGGAGCCATCATCGGCAGTAAATTCGTGACCTTGCTGGATGAA
GAAGACGGAGATACGGAAAAAGCAGCGGACAAGCTGCTGGAAGCCTTGAAAAACTAA

Upstream 100 bases:

>100_bases
GGGCAGGTATCGACCTGAACAGCGGATTCGAGACCGCTCCGGCTCTGAAAGACGACGCCGCCGTCCACACATTTATAAAC
CAATTAAAACAAAAAATACA

Downstream 100 bases:

>100_bases
CCTTTCACAACTCGTAACATCCTCTTCTTCAAAAATCCACCTGAAAGATCATGGCGATACAAGTGAAAAATATAATATCT
TACAGGTATAAGATTATATA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNRINQLFSTKQKDILSIYFCAGFPTLEGTASTIKVLEKKGINMIEIGIPFSDPMADGPVIQHAATRALKNGMTLKLLFD
QLKDIRKEVQIPLVLMGYLNPIMQYGFKDFCRTCRETGIDGVIIPDLPFKDYMEEYRSIAEEQDVRIIMLITPETSEERI
RLIDEHTDGFIYMVSSAAITGAQKDFNAQKQAYFQRIADMNLRNPRMIGFGISNKQTFETASAHAAGAIIGSKFVTLLDE
EDGDTEKAADKLLEALKN

Sequences:

>Translated_258_residues
MNRINQLFSTKQKDILSIYFCAGFPTLEGTASTIKVLEKKGINMIEIGIPFSDPMADGPVIQHAATRALKNGMTLKLLFD
QLKDIRKEVQIPLVLMGYLNPIMQYGFKDFCRTCRETGIDGVIIPDLPFKDYMEEYRSIAEEQDVRIIMLITPETSEERI
RLIDEHTDGFIYMVSSAAITGAQKDFNAQKQAYFQRIADMNLRNPRMIGFGISNKQTFETASAHAAGAIIGSKFVTLLDE
EDGDTEKAADKLLEALKN
>Mature_258_residues
MNRINQLFSTKQKDILSIYFCAGFPTLEGTASTIKVLEKKGINMIEIGIPFSDPMADGPVIQHAATRALKNGMTLKLLFD
QLKDIRKEVQIPLVLMGYLNPIMQYGFKDFCRTCRETGIDGVIIPDLPFKDYMEEYRSIAEEQDVRIIMLITPETSEERI
RLIDEHTDGFIYMVSSAAITGAQKDFNAQKQAYFQRIADMNLRNPRMIGFGISNKQTFETASAHAAGAIIGSKFVTLLDE
EDGDTEKAADKLLEALKN

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=262, Percent_Identity=32.0610687022901, Blast_Score=117, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6321412, Length=261, Percent_Identity=34.0996168582375, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_BACV8 (A6L7N1)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001301317.1
- ProteinModelPortal:   A6L7N1
- SMR:   A6L7N1
- STRING:   A6L7N1
- GeneID:   5305052
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_4093
- NMPDR:   fig|435590.6.peg.3806
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28988; Mature: 28988

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 46-46 ACT_SITE 57-57

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRINQLFSTKQKDILSIYFCAGFPTLEGTASTIKVLEKKGINMIEIGIPFSDPMADGPV
CCHHHHHHHCCCHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
IQHAATRALKNGMTLKLLFDQLKDIRKEVQIPLVLMGYLNPIMQYGFKDFCRTCRETGID
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCC
GVIIPDLPFKDYMEEYRSIAEEQDVRIIMLITPETSEERIRLIDEHTDGFIYMVSSAAIT
EEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEECHHHC
GAQKDFNAQKQAYFQRIADMNLRNPRMIGFGISNKQTFETASAHAAGAIIGSKFVTLLDE
CCHHHCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECC
EDGDTEKAADKLLEALKN
CCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRINQLFSTKQKDILSIYFCAGFPTLEGTASTIKVLEKKGINMIEIGIPFSDPMADGPV
CCHHHHHHHCCCHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
IQHAATRALKNGMTLKLLFDQLKDIRKEVQIPLVLMGYLNPIMQYGFKDFCRTCRETGID
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCC
GVIIPDLPFKDYMEEYRSIAEEQDVRIIMLITPETSEERIRLIDEHTDGFIYMVSSAAIT
EEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEECHHHC
GAQKDFNAQKQAYFQRIADMNLRNPRMIGFGISNKQTFETASAHAAGAIIGSKFVTLLDE
CCHHHCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECC
EDGDTEKAADKLLEALKN
CCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA