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The map label for this gene is pchA [H]

Identifier: 150004956

GI number: 150004956

Start: 3105224

End: 3106366

Strand: Reverse

Name: pchA [H]

Synonym: BVU_2419

Alternate gene names: 150004956

Gene position: 3106366-3105224 (Counterclockwise)

Preceding gene: 150004957

Following gene: 150004955

Centisome position: 60.16

GC content: 50.83

Gene sequence:

>1143_bases
ATGAATCTACCAGATATACATACGCAAAAATTATTGGATTGCCTGACACACAGCCGCCTCGGTTTCGCCCTCTACCGGTT
ACCTTGGACAGACGAATGCTACCTGGTATTGCAAACCTCGGGCGATGTGGAACAATTAGCGGACATTCAGGAACTGAATG
GAAAGAAAGGATTTGTCATGGCTCCTTTCCGGATATCGGAGGAACATCCCTTGGTACTTATCCGTCCGGATGTCACCGCA
TACGACTGGAGCGAAATCAGCGAAGCACTGTCTTCACTGGAATGTGCGGATGCCCTGCTCACCTGCAAAAGCCGGCAGAG
TGAACTCTCCCCTTTTGTTTCCGAAGAAACGGATAAAGAACAATATACCCGCGCATTCGGGCGTTTCATCACTCCCTTAC
AAGAGAAGCGGTTCCAGAAACTGGTTCTGTCACGTTCTTCCGCCAGACATATCGGAGATGATTTTTCACCACTCGGCGCT
TTTGTCCGGGCCTGCAACAACTATCCGCGCATGATGATTTACCTATGCCACACCCCTGCTTCCGGAACCTGGCTGGGCAG
CACACCGGAAATCTTATTAAGCGGACACGGCAAAGAATGGCACACCGTGGCCCTGGCCGGTACCATGCCGATGCAGAATG
AAGTGATGCCGACAGACTGGGACAGGAAGAACCGGGAAGAGCAGGGCTATGTGGCTGATTACATCCGCCGGATCGCCAAA
AGATTCGGCAACAAGATGACGGAGAAAGGTCCTTATACCGCCCGTGCCGGACAATTGGTACACCTCAAGACCGATTTTTA
TTTCTTGTTGAAAAATACAGACCATATAGGCGATTTATTGCAAGAACTCCATCCCACTCCCGCCGTCTGCGGCCTGCCCA
AAGAGGAGGCTTTCCGGTTTATTCCGGACAATGAAGGGTACGACCGCAGTTATTATTCCGGGTTCACCGGCTGGCTGGAC
ACAGAGGGGCATACCGATATCTACGTCAATCTGCGCTGCATGGAAATAAAGCCGGGTGAAGCCATCCTGTATGCAGGAGG
AGGAATACTGGCTTCCTCGGAAGTAGAATCGGAATGGATGGAAACGGGAGACAAGATGAATACGATGCGGAGCATCCTTC
ATCCTGACTTTATAAGTAAATAA

Upstream 100 bases:

>100_bases
ACCGCGGCAGATCCTCGCATATATGGAATATAGATATCACCACTCCACAAGGACAACTGGTTTCTACTGTTCGTGTCGTT
AATTTCATACTGAAAAAGAA

Downstream 100 bases:

>100_bases
ATGGAAACAATTAAAGTAGAAGAATAATGTATTCAGACAAAAAGAACATACTTCAGTTGGTGGCACTTCTCAAAGCGCAC
GGTGTGCGGAAAATAGTGCT

Product: isochorismate synthase EntC

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MNLPDIHTQKLLDCLTHSRLGFALYRLPWTDECYLVLQTSGDVEQLADIQELNGKKGFVMAPFRISEEHPLVLIRPDVTA
YDWSEISEALSSLECADALLTCKSRQSELSPFVSEETDKEQYTRAFGRFITPLQEKRFQKLVLSRSSARHIGDDFSPLGA
FVRACNNYPRMMIYLCHTPASGTWLGSTPEILLSGHGKEWHTVALAGTMPMQNEVMPTDWDRKNREEQGYVADYIRRIAK
RFGNKMTEKGPYTARAGQLVHLKTDFYFLLKNTDHIGDLLQELHPTPAVCGLPKEEAFRFIPDNEGYDRSYYSGFTGWLD
TEGHTDIYVNLRCMEIKPGEAILYAGGGILASSEVESEWMETGDKMNTMRSILHPDFISK

Sequences:

>Translated_380_residues
MNLPDIHTQKLLDCLTHSRLGFALYRLPWTDECYLVLQTSGDVEQLADIQELNGKKGFVMAPFRISEEHPLVLIRPDVTA
YDWSEISEALSSLECADALLTCKSRQSELSPFVSEETDKEQYTRAFGRFITPLQEKRFQKLVLSRSSARHIGDDFSPLGA
FVRACNNYPRMMIYLCHTPASGTWLGSTPEILLSGHGKEWHTVALAGTMPMQNEVMPTDWDRKNREEQGYVADYIRRIAK
RFGNKMTEKGPYTARAGQLVHLKTDFYFLLKNTDHIGDLLQELHPTPAVCGLPKEEAFRFIPDNEGYDRSYYSGFTGWLD
TEGHTDIYVNLRCMEIKPGEAILYAGGGILASSEVESEWMETGDKMNTMRSILHPDFISK
>Mature_380_residues
MNLPDIHTQKLLDCLTHSRLGFALYRLPWTDECYLVLQTSGDVEQLADIQELNGKKGFVMAPFRISEEHPLVLIRPDVTA
YDWSEISEALSSLECADALLTCKSRQSELSPFVSEETDKEQYTRAFGRFITPLQEKRFQKLVLSRSSARHIGDDFSPLGA
FVRACNNYPRMMIYLCHTPASGTWLGSTPEILLSGHGKEWHTVALAGTMPMQNEVMPTDWDRKNREEQGYVADYIRRIAK
RFGNKMTEKGPYTARAGQLVHLKTDFYFLLKNTDHIGDLLQELHPTPAVCGLPKEEAFRFIPDNEGYDRSYYSGFTGWLD
TEGHTDIYVNLRCMEIKPGEAILYAGGGILASSEVESEWMETGDKMNTMRSILHPDFISK

Specific function: Involved in the conversion of chorismate to salicylate (Probable) [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=210, Percent_Identity=30.4761904761905, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87082077, Length=268, Percent_Identity=27.9850746268657, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788114, Length=262, Percent_Identity=25.9541984732824, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 43212; Mature: 43212

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPDIHTQKLLDCLTHSRLGFALYRLPWTDECYLVLQTSGDVEQLADIQELNGKKGFVM
CCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEE
APFRISEEHPLVLIRPDVTAYDWSEISEALSSLECADALLTCKSRQSELSPFVSEETDKE
CCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHH
QYTRAFGRFITPLQEKRFQKLVLSRSSARHIGDDFSPLGAFVRACNNYPRMMIYLCHTPA
HHHHHHHHHHCHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCCEEEEEEEECCC
SGTWLGSTPEILLSGHGKEWHTVALAGTMPMQNEVMPTDWDRKNREEQGYVADYIRRIAK
CCCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RFGNKMTEKGPYTARAGQLVHLKTDFYFLLKNTDHIGDLLQELHPTPAVCGLPKEEAFRF
HHCCCCCCCCCCCCCCCCEEEEECCEEEEEECCHHHHHHHHHHCCCCCCCCCCHHHHEEE
IPDNEGYDRSYYSGFTGWLDTEGHTDIYVNLRCMEIKPGEAILYAGGGILASSEVESEWM
CCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEECCCEEECCHHHHHHH
ETGDKMNTMRSILHPDFISK
HCCHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MNLPDIHTQKLLDCLTHSRLGFALYRLPWTDECYLVLQTSGDVEQLADIQELNGKKGFVM
CCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEE
APFRISEEHPLVLIRPDVTAYDWSEISEALSSLECADALLTCKSRQSELSPFVSEETDKE
CCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHH
QYTRAFGRFITPLQEKRFQKLVLSRSSARHIGDDFSPLGAFVRACNNYPRMMIYLCHTPA
HHHHHHHHHHCHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHCCCCEEEEEEEECCC
SGTWLGSTPEILLSGHGKEWHTVALAGTMPMQNEVMPTDWDRKNREEQGYVADYIRRIAK
CCCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RFGNKMTEKGPYTARAGQLVHLKTDFYFLLKNTDHIGDLLQELHPTPAVCGLPKEEAFRF
HHCCCCCCCCCCCCCCCCEEEEECCEEEEEECCHHHHHHHHHHCCCCCCCCCCHHHHEEE
IPDNEGYDRSYYSGFTGWLDTEGHTDIYVNLRCMEIKPGEAILYAGGGILASSEVESEWM
CCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEECCCEEECCHHHHHHH
ETGDKMNTMRSILHPDFISK
HCCHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7500944; 10984043 [H]