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The map label for this gene is menD

Identifier: 150004955

GI number: 150004955

Start: 3103524

End: 3105197

Strand: Reverse

Name: menD

Synonym: BVU_2418

Alternate gene names: 150004955

Gene position: 3105197-3103524 (Counterclockwise)

Preceding gene: 150004956

Following gene: 150004954

Centisome position: 60.14

GC content: 50.24

Gene sequence:

>1674_bases
ATGTATTCAGACAAAAAGAACATACTTCAGTTGGTGGCACTTCTCAAAGCGCACGGTGTGCGGAAAATAGTGCTTTGTCC
CGGCAGCCGTAATGCTGCCATCGTACATACTCTGGCCAATATAGAGGACTTCACTTGTTATTCTGTAACCGATGAACGCA
GTGCCGGTTTTTTTGCCATCGGCCTATCTTTACAAGGGGGAGGCCCTGCCGCAGTATGCTGCACTTCCGGTTCGGCCCTG
CTGAACCTGCACCCTGCCGTAGCCGAAGCCTTTTACCAACAAGTACCTCTGATTGTCATTTCCGCCGACCGCCCCGCTGC
CTGGATAGGACAAATGGACGGACAAACCCTGCCCCAGCCCCATGTGTTCGGAACGTTAGTCAAGATGTCTGTCAACCTGC
CCGAAGTACATACTGAGGAAGACGAATGGTTTTGCAACCGCCTTATCAACGAAGCCATACTGGAAACCACCCACCACGGC
AAAGGGCCTGTACATATCAATGTACCCATTTCCGAACCCATTTACCGCTTCACCGCCAAAACACTTCCCGAAGTACGTGT
CATCACCCGTTATCAGGGACTGAGCGTGTACGACCGTGACTATAAGGAACTGATCGAACGGCTGAACAAGTACAACAAGC
GCATGGTTGTGGTAGGCCAGATGAATCTGATTTACCTGTTCGAGAAAAAATATGTGAAACCGCTTTATAAACATTTCGCC
TGGCTGACGGAACATCTGGGCAACCAGACCATCCCCGGCATTCCTATCAAGAATTTCGACGCCGCCGTTTATTCCATGAC
ACCGGAACGCCAGGAAGACATGGCCCCCGAAATACTGATTACCTACGGCGGACACATCGTATCCAAACAATTGAAGAAAT
ACCTGCGCAATCATCCCCCGCGCGAACACTGGCATGTTGCCGCCGACGGGAAGATAGCCGATTTATACGGTTGCCTGACC
ACCGTCATCGAGATGGATCCGTTTGAGTTCTTGGAAAAAATAGCTTTCCTGCTGGACAACAAACCTACCCATTATCCGCT
GATGTGGGAAAACTATTGCAAAACCATTCCGATGCCGGACCTTGCTTATTCCGAAATCTCCGTTATCGGAAAACTCATCC
GGGCATTGCCCGAACCCTGTGCCCTGCATCTGGCAAACAGCTCCACCGTGCGCTATGCACAGTTATTTACAGTTCCTCCC
CAGGTGGAAATCTGTTGCAACCGGGGAGTGAACGGCATAGAAGGCTCCCTGTCCACCGCCATCGGTTATGCCGCCGCTTC
CAGCAAGCTGAATTTCATCATCATAGGCGACTTGAGCTTCTTCTATGACATGAACGCACTGTGGAATCAAAATTACGGTG
CCAACATCCGCATCCTGTTACTGAATAACGAAGGAGGAGAAATATTCCACACCCTGCCTGGAATGGACAAATCAAGCCGT
TCCAGGGAGTTTATCACAGCCGAGCATTACACCACCGCCAAAGGCTGGGCGGAAGAACGCGGCTTTATTTACATGAAAGT
GACCGGAGAAGAAGAGCTGGAAGAAGCGATGCAGCCATTCACCTCACCCGAGACACGGATGCAACCCATGCTGCTGGAGG
TGTTTACCGACAAGGAAAAAGACACCACCCTGTTAAGAGAATATTATCACGGACTTAAAAATAAAAATGAATAA

Upstream 100 bases:

>100_bases
CGGAATGGATGGAAACGGGAGACAAGATGAATACGATGCGGAGCATCCTTCATCCTGACTTTATAAGTAAATAAATGGAA
ACAATTAAAGTAGAAGAATA

Downstream 100 bases:

>100_bases
TGGAAACAAGAGAATGGAAAACCATCAAAGAATATCAGGATATTCTGTTTGACTTTTACAACGGAATAGCGAAAATCACC
ATCAACCGTCCGCGTTACCG

Product: 2-oxoglutarate decarboxylase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MYSDKKNILQLVALLKAHGVRKIVLCPGSRNAAIVHTLANIEDFTCYSVTDERSAGFFAIGLSLQGGGPAAVCCTSGSAL
LNLHPAVAEAFYQQVPLIVISADRPAAWIGQMDGQTLPQPHVFGTLVKMSVNLPEVHTEEDEWFCNRLINEAILETTHHG
KGPVHINVPISEPIYRFTAKTLPEVRVITRYQGLSVYDRDYKELIERLNKYNKRMVVVGQMNLIYLFEKKYVKPLYKHFA
WLTEHLGNQTIPGIPIKNFDAAVYSMTPERQEDMAPEILITYGGHIVSKQLKKYLRNHPPREHWHVAADGKIADLYGCLT
TVIEMDPFEFLEKIAFLLDNKPTHYPLMWENYCKTIPMPDLAYSEISVIGKLIRALPEPCALHLANSSTVRYAQLFTVPP
QVEICCNRGVNGIEGSLSTAIGYAAASSKLNFIIIGDLSFFYDMNALWNQNYGANIRILLLNNEGGEIFHTLPGMDKSSR
SREFITAEHYTTAKGWAEERGFIYMKVTGEEELEEAMQPFTSPETRMQPMLLEVFTDKEKDTTLLREYYHGLKNKNE

Sequences:

>Translated_557_residues
MYSDKKNILQLVALLKAHGVRKIVLCPGSRNAAIVHTLANIEDFTCYSVTDERSAGFFAIGLSLQGGGPAAVCCTSGSAL
LNLHPAVAEAFYQQVPLIVISADRPAAWIGQMDGQTLPQPHVFGTLVKMSVNLPEVHTEEDEWFCNRLINEAILETTHHG
KGPVHINVPISEPIYRFTAKTLPEVRVITRYQGLSVYDRDYKELIERLNKYNKRMVVVGQMNLIYLFEKKYVKPLYKHFA
WLTEHLGNQTIPGIPIKNFDAAVYSMTPERQEDMAPEILITYGGHIVSKQLKKYLRNHPPREHWHVAADGKIADLYGCLT
TVIEMDPFEFLEKIAFLLDNKPTHYPLMWENYCKTIPMPDLAYSEISVIGKLIRALPEPCALHLANSSTVRYAQLFTVPP
QVEICCNRGVNGIEGSLSTAIGYAAASSKLNFIIIGDLSFFYDMNALWNQNYGANIRILLLNNEGGEIFHTLPGMDKSSR
SREFITAEHYTTAKGWAEERGFIYMKVTGEEELEEAMQPFTSPETRMQPMLLEVFTDKEKDTTLLREYYHGLKNKNE
>Mature_557_residues
MYSDKKNILQLVALLKAHGVRKIVLCPGSRNAAIVHTLANIEDFTCYSVTDERSAGFFAIGLSLQGGGPAAVCCTSGSAL
LNLHPAVAEAFYQQVPLIVISADRPAAWIGQMDGQTLPQPHVFGTLVKMSVNLPEVHTEEDEWFCNRLINEAILETTHHG
KGPVHINVPISEPIYRFTAKTLPEVRVITRYQGLSVYDRDYKELIERLNKYNKRMVVVGQMNLIYLFEKKYVKPLYKHFA
WLTEHLGNQTIPGIPIKNFDAAVYSMTPERQEDMAPEILITYGGHIVSKQLKKYLRNHPPREHWHVAADGKIADLYGCLT
TVIEMDPFEFLEKIAFLLDNKPTHYPLMWENYCKTIPMPDLAYSEISVIGKLIRALPEPCALHLANSSTVRYAQLFTVPP
QVEICCNRGVNGIEGSLSTAIGYAAASSKLNFIIIGDLSFFYDMNALWNQNYGANIRILLLNNEGGEIFHTLPGMDKSSR
SREFITAEHYTTAKGWAEERGFIYMKVTGEEELEEAMQPFTSPETRMQPMLLEVFTDKEKDTTLLREYYHGLKNKNE

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=568, Percent_Identity=28.6971830985916, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_BACV8 (A6L313)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299699.1
- ProteinModelPortal:   A6L313
- STRING:   A6L313
- GeneID:   5303382
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2418
- NMPDR:   fig|435590.6.peg.2276
- eggNOG:   COG1165
- HOGENOM:   HBG443204
- OMA:   TISLARY
- ProtClustDB:   CLSK2757504
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 62972; Mature: 62972

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSDKKNILQLVALLKAHGVRKIVLCPGSRNAAIVHTLANIEDFTCYSVTDERSAGFFAI
CCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEE
GLSLQGGGPAAVCCTSGSALLNLHPAVAEAFYQQVPLIVISADRPAAWIGQMDGQTLPQP
EEEECCCCCEEEEEECCCEEEEECHHHHHHHHHHCCEEEEECCCCCHHHCCCCCCCCCCC
HVFGTLVKMSVNLPEVHTEEDEWFCNRLINEAILETTHHGKGPVHINVPISEPIYRFTAK
HHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
TLPEVRVITRYQGLSVYDRDYKELIERLNKYNKRMVVVGQMNLIYLFEKKYVKPLYKHFA
CCCCEEEEEEECCCCEECHHHHHHHHHHHHCCCEEEEEECCEEEEEEHHHHHHHHHHHHH
WLTEHLGNQTIPGIPIKNFDAAVYSMTPERQEDMAPEILITYGGHIVSKQLKKYLRNHPP
HHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHCCCCEEEEEECCHHHHHHHHHHHHCCCC
REHWHVAADGKIADLYGCLTTVIEMDPFEFLEKIAFLLDNKPTHYPLMWENYCKTIPMPD
HHHEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCCCCC
LAYSEISVIGKLIRALPEPCALHLANSSTVRYAQLFTVPPQVEICCNRGVNGIEGSLSTA
CHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCCCHHHHHCCCCCCCCCCHHHH
IGYAAASSKLNFIIIGDLSFFYDMNALWNQNYGANIRILLLNNEGGEIFHTLPGMDKSSR
HHHHHHCCCCCEEEEECHHHHHCCHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCCCCC
SREFITAEHYTTAKGWAEERGFIYMKVTGEEELEEAMQPFTSPETRMQPMLLEVFTDKEK
CCCEEEECCEECCCCHHCCCCEEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
DTTLLREYYHGLKNKNE
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYSDKKNILQLVALLKAHGVRKIVLCPGSRNAAIVHTLANIEDFTCYSVTDERSAGFFAI
CCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEE
GLSLQGGGPAAVCCTSGSALLNLHPAVAEAFYQQVPLIVISADRPAAWIGQMDGQTLPQP
EEEECCCCCEEEEEECCCEEEEECHHHHHHHHHHCCEEEEECCCCCHHHCCCCCCCCCCC
HVFGTLVKMSVNLPEVHTEEDEWFCNRLINEAILETTHHGKGPVHINVPISEPIYRFTAK
HHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
TLPEVRVITRYQGLSVYDRDYKELIERLNKYNKRMVVVGQMNLIYLFEKKYVKPLYKHFA
CCCCEEEEEEECCCCEECHHHHHHHHHHHHCCCEEEEEECCEEEEEEHHHHHHHHHHHHH
WLTEHLGNQTIPGIPIKNFDAAVYSMTPERQEDMAPEILITYGGHIVSKQLKKYLRNHPP
HHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHCCCCEEEEEECCHHHHHHHHHHHHCCCC
REHWHVAADGKIADLYGCLTTVIEMDPFEFLEKIAFLLDNKPTHYPLMWENYCKTIPMPD
HHHEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCCCCC
LAYSEISVIGKLIRALPEPCALHLANSSTVRYAQLFTVPPQVEICCNRGVNGIEGSLSTA
CHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCCCHHHHHCCCCCCCCCCHHHH
IGYAAASSKLNFIIIGDLSFFYDMNALWNQNYGANIRILLLNNEGGEIFHTLPGMDKSSR
HHHHHHCCCCCEEEEECHHHHHCCHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCCCCC
SREFITAEHYTTAKGWAEERGFIYMKVTGEEELEEAMQPFTSPETRMQPMLLEVFTDKEK
CCCEEEECCEECCCCHHCCCCEEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
DTTLLREYYHGLKNKNE
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA