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The map label for this gene is rfbB [H]

Identifier: 150004942

GI number: 150004942

Start: 3088618

End: 3089694

Strand: Direct

Name: rfbB [H]

Synonym: BVU_2405

Alternate gene names: 150004942

Gene position: 3088618-3089694 (Clockwise)

Preceding gene: 150004941

Following gene: 150004943

Centisome position: 59.82

GC content: 40.76

Gene sequence:

>1077_bases
ATGGATTCATTCAAACGCAATATAATCATTACCGGTGGTGCCGGTTTTATCGGCAGCCATGTGGTACGTCTTTTTGTAAA
CAAGTATCCGGAATACCGCATTATAAACTTGGATAAACTGACTTATGCCGGTAATCTGGCCAATTTGAAGGATATAGAGG
ACAAACCGAACTATATTTTTGTAAAGGCGGATATTTGTGATTTTGGAACCATTCAGGAATTATTGGTGCGATATCAAGTG
GATGGTATTATCCATCTTGCCGCCGAGAGTCATGTGGACAGAAGTATCAAAGATCCGTTTACTTTTGCACAAACCAATGT
GATGGGTACATTGGCTTTGCTTCAGGCAGCCAAGTTGTATTGGGAATCCCTGCCGGAAAAGTATGAGGGGAAACGCTTCT
ATCATATTTCCACCGATGAAGTATATGGGGCATTGGAATTTGACGGGACTTTCTTCACGGAGGAAACCAAATATCAGCCT
CATAGTCCGTATTCTGCAAGTAAGGCAAGTAGTGATCACTTTGTTCGTGCTTTTCATGACACATATGGTATGCCTACCGT
TGTAACAAACTGTTCTAACAATTATGGTCCTTATCAGTTTCCGGAAAAATTGATTCCGCTTTTTATCAACAATATACGTC
ATGGAAAACCATTGCCGGTATATGGTAGGGGAGAGAACGTACGCGACTGGTTGTATGTTGTGGATCATGCCCGTGCTATT
GATCTTATTTTTCATCAGGGCAGAACAGCGGATACATATAATATCGGTGGTTTCAATGAATGGAAGAATATAGATTTGAT
AAAAGTAATAATCAAAACCGTGGATCGTTTGTTGGGAAATCCGGAAGGCACTTCAGAGTGTCTGATAACTTATGTGACCG
ACCGCAAAGGTCATGATCTCCGCTATGCGATTGACAGCAATAAACTGAAGCACGAATTAGGATGGGAGCCTAGTTTGCAA
TTTGAAGAAGGTATAGAGAGAACGGTCCGTTGGTATTTGGACAATCAGGAATGGATGGATCATATTACCAGTGGCGAATA
TGAAAAATATTATGAGTCGATGTATAAGAACAGATAA

Upstream 100 bases:

>100_bases
AACTTTTGGTATCACTATCCCTTATTGGAAAGATTCTCTAAAGAAATGTATGCTAGAATTGGAAACTCAACAATAACTAA
TTAAAACAATATTTTAAATT

Downstream 100 bases:

>100_bases
TGAGAATCCCAGTTGGGGATAATACGGCATACATCTTTAAACTTACCCCCCATACCACTATGAAAGACAAATTAGCATCC
TACAACCACTTGATCGAGAC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MDSFKRNIIITGGAGFIGSHVVRLFVNKYPEYRIINLDKLTYAGNLANLKDIEDKPNYIFVKADICDFGTIQELLVRYQV
DGIIHLAAESHVDRSIKDPFTFAQTNVMGTLALLQAAKLYWESLPEKYEGKRFYHISTDEVYGALEFDGTFFTEETKYQP
HSPYSASKASSDHFVRAFHDTYGMPTVVTNCSNNYGPYQFPEKLIPLFINNIRHGKPLPVYGRGENVRDWLYVVDHARAI
DLIFHQGRTADTYNIGGFNEWKNIDLIKVIIKTVDRLLGNPEGTSECLITYVTDRKGHDLRYAIDSNKLKHELGWEPSLQ
FEEGIERTVRWYLDNQEWMDHITSGEYEKYYESMYKNR

Sequences:

>Translated_358_residues
MDSFKRNIIITGGAGFIGSHVVRLFVNKYPEYRIINLDKLTYAGNLANLKDIEDKPNYIFVKADICDFGTIQELLVRYQV
DGIIHLAAESHVDRSIKDPFTFAQTNVMGTLALLQAAKLYWESLPEKYEGKRFYHISTDEVYGALEFDGTFFTEETKYQP
HSPYSASKASSDHFVRAFHDTYGMPTVVTNCSNNYGPYQFPEKLIPLFINNIRHGKPLPVYGRGENVRDWLYVVDHARAI
DLIFHQGRTADTYNIGGFNEWKNIDLIKVIIKTVDRLLGNPEGTSECLITYVTDRKGHDLRYAIDSNKLKHELGWEPSLQ
FEEGIERTVRWYLDNQEWMDHITSGEYEKYYESMYKNR
>Mature_358_residues
MDSFKRNIIITGGAGFIGSHVVRLFVNKYPEYRIINLDKLTYAGNLANLKDIEDKPNYIFVKADICDFGTIQELLVRYQV
DGIIHLAAESHVDRSIKDPFTFAQTNVMGTLALLQAAKLYWESLPEKYEGKRFYHISTDEVYGALEFDGTFFTEETKYQP
HSPYSASKASSDHFVRAFHDTYGMPTVVTNCSNNYGPYQFPEKLIPLFINNIRHGKPLPVYGRGENVRDWLYVVDHARAI
DLIFHQGRTADTYNIGGFNEWKNIDLIKVIIKTVDRLLGNPEGTSECLITYVTDRKGHDLRYAIDSNKLKHELGWEPSLQ
FEEGIERTVRWYLDNQEWMDHITSGEYEKYYESMYKNR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=40.0602409638554, Blast_Score=252, Evalue=3e-67,
Organism=Homo sapiens, GI42516563, Length=340, Percent_Identity=24.7058823529412, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI56237023, Length=361, Percent_Identity=24.3767313019391, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI56118217, Length=361, Percent_Identity=24.3767313019391, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI189083684, Length=361, Percent_Identity=24.3767313019391, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788353, Length=360, Percent_Identity=53.6111111111111, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=54.178674351585, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788366, Length=361, Percent_Identity=25.207756232687, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786974, Length=354, Percent_Identity=24.8587570621469, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=37.9939209726444, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=32.3262839879154, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71982035, Length=369, Percent_Identity=26.5582655826558, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=371, Percent_Identity=26.4150943396226, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=23.9520958083832, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI133901786, Length=352, Percent_Identity=23.2954545454545, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=352, Percent_Identity=23.2954545454545, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=352, Percent_Identity=23.2954545454545, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=352, Percent_Identity=23.2954545454545, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=352, Percent_Identity=23.2954545454545, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=348, Percent_Identity=24.1379310344828, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=358, Percent_Identity=25.9776536312849, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19923002, Length=361, Percent_Identity=25.4847645429363, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=25.7396449704142, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 41494; Mature: 41494

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSFKRNIIITGGAGFIGSHVVRLFVNKYPEYRIINLDKLTYAGNLANLKDIEDKPNYIF
CCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHEEECCCCCCCCCCCCCCCEEE
VKADICDFGTIQELLVRYQVDGIIHLAAESHVDRSIKDPFTFAQTNVMGTLALLQAAKLY
EEECCCCCHHHHHHHHHHHHCCEEEEEEHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHH
WESLPEKYEGKRFYHISTDEVYGALEFDGTFFTEETKYQPHSPYSASKASSDHFVRAFHD
HHHCCHHHCCCEEEEEEHHHEEEEEEECCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
TYGMPTVVTNCSNNYGPYQFPEKLIPLFINNIRHGKPLPVYGRGENVRDWLYVVDHARAI
HCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHE
DLIFHQGRTADTYNIGGFNEWKNIDLIKVIIKTVDRLLGNPEGTSECLITYVTDRKGHDL
EHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCE
RYAIDSNKLKHELGWEPSLQFEEGIERTVRWYLDNQEWMDHITSGEYEKYYESMYKNR
EEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MDSFKRNIIITGGAGFIGSHVVRLFVNKYPEYRIINLDKLTYAGNLANLKDIEDKPNYIF
CCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHEEECCCCCCCCCCCCCCCEEE
VKADICDFGTIQELLVRYQVDGIIHLAAESHVDRSIKDPFTFAQTNVMGTLALLQAAKLY
EEECCCCCHHHHHHHHHHHHCCEEEEEEHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHH
WESLPEKYEGKRFYHISTDEVYGALEFDGTFFTEETKYQPHSPYSASKASSDHFVRAFHD
HHHCCHHHCCCEEEEEEHHHEEEEEEECCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
TYGMPTVVTNCSNNYGPYQFPEKLIPLFINNIRHGKPLPVYGRGENVRDWLYVVDHARAI
HCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHE
DLIFHQGRTADTYNIGGFNEWKNIDLIKVIIKTVDRLLGNPEGTSECLITYVTDRKGHDL
EHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCE
RYAIDSNKLKHELGWEPSLQFEEGIERTVRWYLDNQEWMDHITSGEYEKYYESMYKNR
EEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]