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The map label for this gene is wcaJ [H]

Identifier: 150004943

GI number: 150004943

Start: 3089694

End: 3091169

Strand: Direct

Name: wcaJ [H]

Synonym: BVU_2406

Alternate gene names: 150004943

Gene position: 3089694-3091169 (Clockwise)

Preceding gene: 150004942

Following gene: 150004948

Centisome position: 59.84

GC content: 48.85

Gene sequence:

>1476_bases
ATGAGAATCCCAGTTGGGGATAATACGGCATACATCTTTAAACTTACCCCCCATACCACTATGAAAGACAAATTAGCATC
CTACAACCACTTGATCGAGACCTTTGTGATTGTCACCGAGACCTTTCTTTGCGGTCTGCTATTCCTGCTTTTCAGCAAGC
TGTCCAATGAGATGCAGTGGGATAGCCTGCAGGTTGGTGGAACCACCGTTCTGCAAGTCATGCTTACACTGATGTTATGT
TACGCCCTTTGCGCCATCCATTCAGGAGTAGTGCTTCACCGGAGGAAAGTGCATTCCCTTCAGATATGGAAACGTGTGTT
CGAGAATATGTTCCTGTTTGTCCTCCTGGGCGGTCTGGTCCTTTCTGTAGGAAACTATGCGGATATAGCCTCCCTGTTCA
TGCTGGAGTACCTTTTCCTCCTGTTTCTGTGTCTTGTTTCGTTCCGTTTCACTCTTCGTCTTCTCATCAGGCTTTACCGT
ATGAGCAAGAAACATACGCGTTTTGTAGTACTGGTAGGAAGTACGGACAACAACCTGGAAATCTACCATGAGATGTCGGG
CAGTGAAGATACAGGTTATTCGGTCGTGGGCTATTTTGACGGTCAGGCGAATCCTGCTTTCCCGGTAGAATGTCCGTATT
TGGGCCAGCCGGCGCAGGTACAGGAATATCTGGAGAAACATGATTATGTACATTACCTGTTCTGCTGCCTGCCCTCAAAA
GACAGAGAGGTCATCGTTTCTCTGATTGATTACTGCGAGAACCATCTGGTACATTTCTTCAGTGTGCCCAATGTGCGCAA
TTATCTCCACCACCGTATGTCGTTCAACATCATGGGGAACGTCCCTTACCTGGGCCTTCGCCCCGATCCCTTAAGTTGGC
CGGGGAACCGCCTGCTGAAAAGAGCCTTTGACATAGTAGTCTCATCGGTGTTCCTTTGTACGTTTTTTCCGGTTATTCTG
ATTGTGGTGGCGATAGTGACCGGATTGACCATGCCCGGCCCGTTGTTCTTCCGCCAGAAACGGAACGGGCTGAACGGCAG
GGAGTTTTATTGTTACAAGTTCCGCAGTATGAAAGTAAATGCAGATGCCGACAGGATACAGGCCACGGAGCATGATCCCC
GCAAGACGCGCTGGGGCAATATAATGCGTAAGACGAATATAGATGAACTGCCCCAGTTTATCAATGTGCTGTTGGGGGAT
ATGAGTATCGTGGGGCCTCGTCCTCACATGCTTCTCCATACGCAGGAATATTCAAGGCTGATCAATAAATATATGGTGCG
GCATTTTGTGAAACCGGGTATAACGGGATGGAGCCAGGTTACGGGTTTCCGTGGGGAAACGAAGGAACTAAAAGACATGG
AGGGCCGTATCCGTGGTGATATCTGGTATTTGGAGCACTGGAGCTTCGGTCTGGACCTTTACATCATGTACAGGACGGTG
GCGAATGTCTTCCGTGGCGAGAAGAATGCCTATTAG

Upstream 100 bases:

>100_bases
AGAGAACGGTCCGTTGGTATTTGGACAATCAGGAATGGATGGATCATATTACCAGTGGCGAATATGAAAAATATTATGAG
TCGATGTATAAGAACAGATA

Downstream 100 bases:

>100_bases
TAAAACGGGCTGTTCTTAACGATCCTTCTCGATGAGGGTCCTTAGTAATTTAGCAGTTAAAAAACTTTGTTTTTGCAGCC
TGTTATTTCCGTCTGCCGGC

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MRIPVGDNTAYIFKLTPHTTMKDKLASYNHLIETFVIVTETFLCGLLFLLFSKLSNEMQWDSLQVGGTTVLQVMLTLMLC
YALCAIHSGVVLHRRKVHSLQIWKRVFENMFLFVLLGGLVLSVGNYADIASLFMLEYLFLLFLCLVSFRFTLRLLIRLYR
MSKKHTRFVVLVGSTDNNLEIYHEMSGSEDTGYSVVGYFDGQANPAFPVECPYLGQPAQVQEYLEKHDYVHYLFCCLPSK
DREVIVSLIDYCENHLVHFFSVPNVRNYLHHRMSFNIMGNVPYLGLRPDPLSWPGNRLLKRAFDIVVSSVFLCTFFPVIL
IVVAIVTGLTMPGPLFFRQKRNGLNGREFYCYKFRSMKVNADADRIQATEHDPRKTRWGNIMRKTNIDELPQFINVLLGD
MSIVGPRPHMLLHTQEYSRLINKYMVRHFVKPGITGWSQVTGFRGETKELKDMEGRIRGDIWYLEHWSFGLDLYIMYRTV
ANVFRGEKNAY

Sequences:

>Translated_491_residues
MRIPVGDNTAYIFKLTPHTTMKDKLASYNHLIETFVIVTETFLCGLLFLLFSKLSNEMQWDSLQVGGTTVLQVMLTLMLC
YALCAIHSGVVLHRRKVHSLQIWKRVFENMFLFVLLGGLVLSVGNYADIASLFMLEYLFLLFLCLVSFRFTLRLLIRLYR
MSKKHTRFVVLVGSTDNNLEIYHEMSGSEDTGYSVVGYFDGQANPAFPVECPYLGQPAQVQEYLEKHDYVHYLFCCLPSK
DREVIVSLIDYCENHLVHFFSVPNVRNYLHHRMSFNIMGNVPYLGLRPDPLSWPGNRLLKRAFDIVVSSVFLCTFFPVIL
IVVAIVTGLTMPGPLFFRQKRNGLNGREFYCYKFRSMKVNADADRIQATEHDPRKTRWGNIMRKTNIDELPQFINVLLGD
MSIVGPRPHMLLHTQEYSRLINKYMVRHFVKPGITGWSQVTGFRGETKELKDMEGRIRGDIWYLEHWSFGLDLYIMYRTV
ANVFRGEKNAY
>Mature_491_residues
MRIPVGDNTAYIFKLTPHTTMKDKLASYNHLIETFVIVTETFLCGLLFLLFSKLSNEMQWDSLQVGGTTVLQVMLTLMLC
YALCAIHSGVVLHRRKVHSLQIWKRVFENMFLFVLLGGLVLSVGNYADIASLFMLEYLFLLFLCLVSFRFTLRLLIRLYR
MSKKHTRFVVLVGSTDNNLEIYHEMSGSEDTGYSVVGYFDGQANPAFPVECPYLGQPAQVQEYLEKHDYVHYLFCCLPSK
DREVIVSLIDYCENHLVHFFSVPNVRNYLHHRMSFNIMGNVPYLGLRPDPLSWPGNRLLKRAFDIVVSSVFLCTFFPVIL
IVVAIVTGLTMPGPLFFRQKRNGLNGREFYCYKFRSMKVNADADRIQATEHDPRKTRWGNIMRKTNIDELPQFINVLLGD
MSIVGPRPHMLLHTQEYSRLINKYMVRHFVKPGITGWSQVTGFRGETKELKDMEGRIRGDIWYLEHWSFGLDLYIMYRTV
ANVFRGEKNAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=403, Percent_Identity=36.4764267990074, Blast_Score=224, Evalue=8e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 56972; Mature: 56972

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPVGDNTAYIFKLTPHTTMKDKLASYNHLIETFVIVTETFLCGLLFLLFSKLSNEMQW
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DSLQVGGTTVLQVMLTLMLCYALCAIHSGVVLHRRKVHSLQIWKRVFENMFLFVLLGGLV
CCEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVGNYADIASLFMLEYLFLLFLCLVSFRFTLRLLIRLYRMSKKHTRFVVLVGSTDNNLE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
IYHEMSGSEDTGYSVVGYFDGQANPAFPVECPYLGQPAQVQEYLEKHDYVHYLFCCLPSK
EEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCC
DREVIVSLIDYCENHLVHFFSVPNVRNYLHHRMSFNIMGNVPYLGLRPDPLSWPGNRLLK
CHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHEEEEECCCCEECCCCCCCCCCHHHHHH
RAFDIVVSSVFLCTFFPVILIVVAIVTGLTMPGPLFFRQKRNGLNGREFYCYKFRSMKVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCEEEEEEEEEEEEC
ADADRIQATEHDPRKTRWGNIMRKTNIDELPQFINVLLGDMSIVGPRPHMLLHTQEYSRL
CCHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHH
INKYMVRHFVKPGITGWSQVTGFRGETKELKDMEGRIRGDIWYLEHWSFGLDLYIMYRTV
HHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH
ANVFRGEKNAY
HHHHCCCCCCC
>Mature Secondary Structure
MRIPVGDNTAYIFKLTPHTTMKDKLASYNHLIETFVIVTETFLCGLLFLLFSKLSNEMQW
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DSLQVGGTTVLQVMLTLMLCYALCAIHSGVVLHRRKVHSLQIWKRVFENMFLFVLLGGLV
CCEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVGNYADIASLFMLEYLFLLFLCLVSFRFTLRLLIRLYRMSKKHTRFVVLVGSTDNNLE
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
IYHEMSGSEDTGYSVVGYFDGQANPAFPVECPYLGQPAQVQEYLEKHDYVHYLFCCLPSK
EEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCC
DREVIVSLIDYCENHLVHFFSVPNVRNYLHHRMSFNIMGNVPYLGLRPDPLSWPGNRLLK
CHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHEEEEECCCCEECCCCCCCCCCHHHHHH
RAFDIVVSSVFLCTFFPVILIVVAIVTGLTMPGPLFFRQKRNGLNGREFYCYKFRSMKVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCEEEEEEEEEEEEC
ADADRIQATEHDPRKTRWGNIMRKTNIDELPQFINVLLGDMSIVGPRPHMLLHTQEYSRL
CCHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHH
INKYMVRHFVKPGITGWSQVTGFRGETKELKDMEGRIRGDIWYLEHWSFGLDLYIMYRTV
HHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH
ANVFRGEKNAY
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]