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The map label for this gene is spsK [H]

Identifier: 150004941

GI number: 150004941

Start: 3087733

End: 3088593

Strand: Direct

Name: spsK [H]

Synonym: BVU_2404

Alternate gene names: 150004941

Gene position: 3087733-3088593 (Clockwise)

Preceding gene: 150004940

Following gene: 150004942

Centisome position: 59.8

GC content: 41.58

Gene sequence:

>861_bases
ATGAATATACTTGTTACAGGTGCCAATGGTCAACTTGGCAATGAGATGCGCAGGGTGTCGTTAGACAGCCGGAATCGTTA
TCTCTTTACAGATGTAAATGAACTGGATATCACAGATGCGACAGCTGTCCGTAATATGTTGAAAAAAGAGCAGATAGATG
TGATTGTAAATTGTGCTGCTTATACGAATGTAGACAGGGCGGAAGATGATTTTGCTATGGCTGATTTGCTGAATAACAAA
GCTGTGGAGAATTTGGCTGTTGCTGCCGCAGAAACAGGTGCCACGCTTATTCATGTATCTACAGACTATGTGTTCCGAGG
GGATAAAAACATTCCTTGCCGTGAGTCTTGGGAAACTGATCCGTTGGGTGTCTATGGAAAAACCAAACTGGCGGGCGAAC
AGTCTTTGGTAAAAGCAGGATGTAAATATCTTATTTTCCGCACAGCTTGGCTTTACTCGCCTTTCGGTAAGAACTTCGTG
AAAACCATGCGGCAATTGACTTCAACAAAAGATTCACTGAAAGTGGTTTTTGATCAAGTAGGCACTCCTACTTATGCCGG
AGATTTGGCTGCTTTGATCTATAACATAATCGAAGAGAACTTGCTGGATCGTCAAGGTGTCTATCATTTCAGCAATGAAG
GGGTATGTTCTTGGTATGATTTTGCGAAGGAAATTTGTGAATTGAGCGGAAATAATTGTGACATACAGCCTTGTCACAGC
GATGAGTTCCCCAGTAAGGTGGGACGTCCTCATTTTTCGGTGCTGGATAAAACAAAAGTGAAGGAAACTTTTGGTATCAC
TATCCCTTATTGGAAAGATTCTCTAAAGAAATGTATGCTAGAATTGGAAACTCAACAATAA

Upstream 100 bases:

>100_bases
TACCCGTTGCCGAGGCCATTTTAAGTGAGAAGGATTTGAAACATCCTTTGTTGAAAGACTATGACTCTCCGTTTGACTAC
ACTATTAATCTATACGAATG

Downstream 100 bases:

>100_bases
CTAATTAAAACAATATTTTAAATTATGGATTCATTCAAACGCAATATAATCATTACCGGTGGTGCCGGTTTTATCGGCAG
CCATGTGGTACGTCTTTTTG

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-L-rhamnose; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNILVTGANGQLGNEMRRVSLDSRNRYLFTDVNELDITDATAVRNMLKKEQIDVIVNCAAYTNVDRAEDDFAMADLLNNK
AVENLAVAAAETGATLIHVSTDYVFRGDKNIPCRESWETDPLGVYGKTKLAGEQSLVKAGCKYLIFRTAWLYSPFGKNFV
KTMRQLTSTKDSLKVVFDQVGTPTYAGDLAALIYNIIEENLLDRQGVYHFSNEGVCSWYDFAKEICELSGNNCDIQPCHS
DEFPSKVGRPHFSVLDKTKVKETFGITIPYWKDSLKKCMLELETQQ

Sequences:

>Translated_286_residues
MNILVTGANGQLGNEMRRVSLDSRNRYLFTDVNELDITDATAVRNMLKKEQIDVIVNCAAYTNVDRAEDDFAMADLLNNK
AVENLAVAAAETGATLIHVSTDYVFRGDKNIPCRESWETDPLGVYGKTKLAGEQSLVKAGCKYLIFRTAWLYSPFGKNFV
KTMRQLTSTKDSLKVVFDQVGTPTYAGDLAALIYNIIEENLLDRQGVYHFSNEGVCSWYDFAKEICELSGNNCDIQPCHS
DEFPSKVGRPHFSVLDKTKVKETFGITIPYWKDSLKKCMLELETQQ
>Mature_286_residues
MNILVTGANGQLGNEMRRVSLDSRNRYLFTDVNELDITDATAVRNMLKKEQIDVIVNCAAYTNVDRAEDDFAMADLLNNK
AVENLAVAAAETGATLIHVSTDYVFRGDKNIPCRESWETDPLGVYGKTKLAGEQSLVKAGCKYLIFRTAWLYSPFGKNFV
KTMRQLTSTKDSLKVVFDQVGTPTYAGDLAALIYNIIEENLLDRQGVYHFSNEGVCSWYDFAKEICELSGNNCDIQPCHS
DEFPSKVGRPHFSVLDKTKVKETFGITIPYWKDSLKKCMLELETQQ

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=281, Percent_Identity=32.3843416370107, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI11034825, Length=281, Percent_Identity=32.3843416370107, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1788352, Length=298, Percent_Identity=38.9261744966443, Blast_Score=194, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133 [C]

Molecular weight: Translated: 32146; Mature: 32146

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGANGQLGNEMRRVSLDSRNRYLFTDVNELDITDATAVRNMLKKEQIDVIVNCAA
CEEEEECCCCCCCHHHHHEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEHH
YTNVDRAEDDFAMADLLNNKAVENLAVAAAETGATLIHVSTDYVFRGDKNIPCRESWETD
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCCCCCCCCCC
PLGVYGKTKLAGEQSLVKAGCKYLIFRTAWLYSPFGKNFVKTMRQLTSTKDSLKVVFDQV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHC
GTPTYAGDLAALIYNIIEENLLDRQGVYHFSNEGVCSWYDFAKEICELSGNNCDIQPCHS
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
DEFPSKVGRPHFSVLDKTKVKETFGITIPYWKDSLKKCMLELETQQ
CCCCHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNILVTGANGQLGNEMRRVSLDSRNRYLFTDVNELDITDATAVRNMLKKEQIDVIVNCAA
CEEEEECCCCCCCHHHHHEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEHH
YTNVDRAEDDFAMADLLNNKAVENLAVAAAETGATLIHVSTDYVFRGDKNIPCRESWETD
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCCCCCCCCCC
PLGVYGKTKLAGEQSLVKAGCKYLIFRTAWLYSPFGKNFVKTMRQLTSTKDSLKVVFDQV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHC
GTPTYAGDLAALIYNIIEENLLDRQGVYHFSNEGVCSWYDFAKEICELSGNNCDIQPCHS
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
DEFPSKVGRPHFSVLDKTKVKETFGITIPYWKDSLKKCMLELETQQ
CCCCHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-L-mannose; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-L-mannose + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> dTDP-L-rhamnose + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]