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The map label for this gene is folD [H]

Identifier: 150004754

GI number: 150004754

Start: 2861382

End: 2862263

Strand: Direct

Name: folD [H]

Synonym: BVU_2215

Alternate gene names: 150004754

Gene position: 2861382-2862263 (Clockwise)

Preceding gene: 150004753

Following gene: 150004755

Centisome position: 55.42

GC content: 45.58

Gene sequence:

>882_bases
ATGCAACTAATTGATGGAAAAGCTATTTCGGAACTGGTGAAGCAGGAGATTGCTGCGGAAGTTGCCGGAATCGTGGCTAA
AGGTGGCAAGCGTCCCCATTTAGCTGCAATCCTCGTTGGACACGACGGGGGAAGTGAGACATACGTGGCGGCTAAAGTAA
AGGCCTGTGAAGTATGTGGTTTCAAATCCAGTCTGATCCGTTATGAAGCAGATGTGACGGAAGAAGAATTACTGGCAAAG
GTCAGAGAACTGAATGAGGATGCTGACGTGGATGGTTTTATCGTACAACTTCCCTTACCGAAGCATATTTCCGAGCAGAA
AGTGATTGAAACCATTGACTATCGGAAAGATGTGGATGGTTTTCACCCTATCAATGTAGGTCGTATGTCCATTGGTCTTC
CTTGCTATGTATCTGCTACTCCTAATGGTATTCTGGAATTGCTGAAGCGTTACCATATTGAAACACAGGGAAAGAAGTGT
GTAGTATTGGGCCGTAGCAATATTGTGGGTAAGCCTATGGCGAGCCTGATGATGCAGAAAGCTTATCCGGGTGATGCCAC
TGTAACTGTTTGTCACAGCCGCAGCAAGGATTTGGTAAAAGAATGTCAGGAAGCTGACATTATCATTGCTGCACTGGGTC
AGCCGAATTTTGTGAAGGCTGAGATGGTGAAAGAAGGTGCGGTGGTTATTGACGTGGGTACTACCCGTGTGCCGGATGCC
ACAAAGAAATCCGGATTCAAACTGACCGGAGATGTGAAATTTGATGAAGTGGCTCCCAAATGTTCTTATATCACTCCTGT
TCCGGGTGGTGTGGGACCTATGACTATTGTATCTTTAATGAAGAATACGTTGCTGGCTGGAAAAAAGGCCATTTACAAAT
AA

Upstream 100 bases:

>100_bases
AGGTAGGGAGAAAGTGCGGAACTTTCTCCTTTTTTTATTATGCAACGTGGCTGATTTATGCTACATTTGTTGCTCAATGG
ATTATTTATAAATAAATAGT

Downstream 100 bases:

>100_bases
GTGCCACACGCTTTGCGAAGCTTGCTGGATAAAAAAGAATGTGTCAAAAGTTTGGTAATGCTTACAAGATAAGCTGACCT
GCTTTTGACACACTTGTTTT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MQLIDGKAISELVKQEIAAEVAGIVAKGGKRPHLAAILVGHDGGSETYVAAKVKACEVCGFKSSLIRYEADVTEEELLAK
VRELNEDADVDGFIVQLPLPKHISEQKVIETIDYRKDVDGFHPINVGRMSIGLPCYVSATPNGILELLKRYHIETQGKKC
VVLGRSNIVGKPMASLMMQKAYPGDATVTVCHSRSKDLVKECQEADIIIAALGQPNFVKAEMVKEGAVVIDVGTTRVPDA
TKKSGFKLTGDVKFDEVAPKCSYITPVPGGVGPMTIVSLMKNTLLAGKKAIYK

Sequences:

>Translated_293_residues
MQLIDGKAISELVKQEIAAEVAGIVAKGGKRPHLAAILVGHDGGSETYVAAKVKACEVCGFKSSLIRYEADVTEEELLAK
VRELNEDADVDGFIVQLPLPKHISEQKVIETIDYRKDVDGFHPINVGRMSIGLPCYVSATPNGILELLKRYHIETQGKKC
VVLGRSNIVGKPMASLMMQKAYPGDATVTVCHSRSKDLVKECQEADIIIAALGQPNFVKAEMVKEGAVVIDVGTTRVPDA
TKKSGFKLTGDVKFDEVAPKCSYITPVPGGVGPMTIVSLMKNTLLAGKKAIYK
>Mature_293_residues
MQLIDGKAISELVKQEIAAEVAGIVAKGGKRPHLAAILVGHDGGSETYVAAKVKACEVCGFKSSLIRYEADVTEEELLAK
VRELNEDADVDGFIVQLPLPKHISEQKVIETIDYRKDVDGFHPINVGRMSIGLPCYVSATPNGILELLKRYHIETQGKKC
VVLGRSNIVGKPMASLMMQKAYPGDATVTVCHSRSKDLVKECQEADIIIAALGQPNFVKAEMVKEGAVVIDVGTTRVPDA
TKKSGFKLTGDVKFDEVAPKCSYITPVPGGVGPMTIVSLMKNTLLAGKKAIYK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=48.4745762711864, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=46.8013468013468, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI222136639, Length=297, Percent_Identity=41.0774410774411, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI36796743, Length=203, Percent_Identity=25.615763546798, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786741, Length=285, Percent_Identity=45.9649122807018, Blast_Score=246, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17568735, Length=267, Percent_Identity=39.7003745318352, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319558, Length=291, Percent_Identity=44.6735395189003, Blast_Score=256, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6321643, Length=301, Percent_Identity=43.8538205980066, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322933, Length=302, Percent_Identity=28.1456953642384, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=46.5986394557823, Blast_Score=263, Evalue=8e-71,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=46.5986394557823, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24645718, Length=296, Percent_Identity=39.8648648648649, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI17137370, Length=296, Percent_Identity=39.8648648648649, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI62472483, Length=296, Percent_Identity=39.8648648648649, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI45551871, Length=296, Percent_Identity=39.8648648648649, Blast_Score=211, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31534; Mature: 31534

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLIDGKAISELVKQEIAAEVAGIVAKGGKRPHLAAILVGHDGGSETYVAAKVKACEVCG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEHHHHHCC
FKSSLIRYEADVTEEELLAKVRELNEDADVDGFIVQLPLPKHISEQKVIETIDYRKDVDG
CHHHHHEEECCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
FHPINVGRMSIGLPCYVSATPNGILELLKRYHIETQGKKCVVLGRSNIVGKPMASLMMQK
CCCCCCCEEEECCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHH
AYPGDATVTVCHSRSKDLVKECQEADIIIAALGQPNFVKAEMVKEGAVVIDVGTTRVPDA
CCCCCCEEEEECCCCHHHHHHHHHCCEEEEECCCCCCCHHHHHHCCCEEEEECCCCCCCC
TKKSGFKLTGDVKFDEVAPKCSYITPVPGGVGPMTIVSLMKNTLLAGKKAIYK
HHHCCEEEECCCCHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLIDGKAISELVKQEIAAEVAGIVAKGGKRPHLAAILVGHDGGSETYVAAKVKACEVCG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEHHHHHCC
FKSSLIRYEADVTEEELLAKVRELNEDADVDGFIVQLPLPKHISEQKVIETIDYRKDVDG
CHHHHHEEECCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
FHPINVGRMSIGLPCYVSATPNGILELLKRYHIETQGKKCVVLGRSNIVGKPMASLMMQK
CCCCCCCEEEECCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHH
AYPGDATVTVCHSRSKDLVKECQEADIIIAALGQPNFVKAEMVKEGAVVIDVGTTRVPDA
CCCCCCEEEEECCCCHHHHHHHHHCCEEEEECCCCCCCHHHHHHCCCEEEEECCCCCCCC
TKKSGFKLTGDVKFDEVAPKCSYITPVPGGVGPMTIVSLMKNTLLAGKKAIYK
HHHCCEEEECCCCHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA