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The map label for this gene is mscS [H]

Identifier: 150004544

GI number: 150004544

Start: 2581158

End: 2581991

Strand: Reverse

Name: mscS [H]

Synonym: BVU_1994

Alternate gene names: 150004544

Gene position: 2581991-2581158 (Counterclockwise)

Preceding gene: 150004545

Following gene: 150004541

Centisome position: 50.01

GC content: 39.21

Gene sequence:

>834_bases
ATGTTATTAGCATCTGAAACAAGTATTCAATTAACCAATGCATTAGAGAAACTTATCGACTCCGGCATTCAATTAGGAGA
AAGAGTATTAGGAGCACTTATTATTTTTATAATCGGAAAGTTCCTCGTAAATTGGCTGAATAGACTTTTTGCACGTATTT
TGGAAAAAAGAAAAGTGGACCCGAGTATCCAGTCATTCCTAAAGAGTATGGTGAATATCTTACTGCTCATCATGCTGATT
CTTGCTGTGATAGGGAAATTAGGTATCGAGCTGACAGGATTCGCCGCACTTCTCGCTTCTGCCGGTGTTGCCATCGGTAT
GGCACTGTCTGGTAACTTACAAAATTTTGCCGGTGGACTGATTATCTTATTGTTCAGGCCTTATAAAGTGGGGGACTTTA
TCGAAGCTTCTACCGGTGCCTCGGGAACAGTAAAAGAAATACAGATTTTTCATACTATTCTTATCACTGCAGACAACAAG
ATGATATATGTACCTAATGGAGCCATGAGTAGTGGGGTCATTACAAATTACAGCAAACTGGACACCCGCCGTATAGAATG
GAATTTTGGCGTGGACTACGGAGAAGACTATAATAAAGTAGAAAAAGTATTGCGCGAAATCATTGCCAATGATAAACGTA
TTCTCACCAGTCCTGCCCCTTTTATTGAATTAGGAGAACTTGCCGCCAGCAGTGTAAATATAAAAATCCGCGTATGGGTG
AATAGTTCGGATTACTGGAACGTATTTTTCAGTATGAATCAGACTGTCTATACTATTTTTAATAAAGAAGGAATTAACTT
CCCATTCCCACAGCTTACTGTACATCAAGCCTAA

Upstream 100 bases:

>100_bases
CGGAGAAGCAGAAATTGTACGTCAAGGTAAAGGTATTCTGAACGATTTTTAATCTGAATTGTTTTATATATTAGAAATGT
CAAATTAAAGTAAATTTATC

Downstream 100 bases:

>100_bases
CGTCAGAAATCTTTAGAACACGAATGACGCAATTGTCACAAGCTTGATCTTATCAATCATGTGTCATTTGCGTCATTTGT
ATGCTATCACAAGATTTATT

Product: putative transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLLASETSIQLTNALEKLIDSGIQLGERVLGALIIFIIGKFLVNWLNRLFARILEKRKVDPSIQSFLKSMVNILLLIMLI
LAVIGKLGIELTGFAALLASAGVAIGMALSGNLQNFAGGLIILLFRPYKVGDFIEASTGASGTVKEIQIFHTILITADNK
MIYVPNGAMSSGVITNYSKLDTRRIEWNFGVDYGEDYNKVEKVLREIIANDKRILTSPAPFIELGELAASSVNIKIRVWV
NSSDYWNVFFSMNQTVYTIFNKEGINFPFPQLTVHQA

Sequences:

>Translated_277_residues
MLLASETSIQLTNALEKLIDSGIQLGERVLGALIIFIIGKFLVNWLNRLFARILEKRKVDPSIQSFLKSMVNILLLIMLI
LAVIGKLGIELTGFAALLASAGVAIGMALSGNLQNFAGGLIILLFRPYKVGDFIEASTGASGTVKEIQIFHTILITADNK
MIYVPNGAMSSGVITNYSKLDTRRIEWNFGVDYGEDYNKVEKVLREIIANDKRILTSPAPFIELGELAASSVNIKIRVWV
NSSDYWNVFFSMNQTVYTIFNKEGINFPFPQLTVHQA
>Mature_277_residues
MLLASETSIQLTNALEKLIDSGIQLGERVLGALIIFIIGKFLVNWLNRLFARILEKRKVDPSIQSFLKSMVNILLLIMLI
LAVIGKLGIELTGFAALLASAGVAIGMALSGNLQNFAGGLIILLFRPYKVGDFIEASTGASGTVKEIQIFHTILITADNK
MIYVPNGAMSSGVITNYSKLDTRRIEWNFGVDYGEDYNKVEKVLREIIANDKRILTSPAPFIELGELAASSVNIKIRVWV
NSSDYWNVFFSMNQTVYTIFNKEGINFPFPQLTVHQA

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=247, Percent_Identity=36.0323886639676, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1786670, Length=210, Percent_Identity=31.4285714285714, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787591, Length=228, Percent_Identity=28.9473684210526, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI2367355, Length=245, Percent_Identity=26.530612244898, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 30664; Mature: 30664

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLASETSIQLTNALEKLIDSGIQLGERVLGALIIFIIGKFLVNWLNRLFARILEKRKVD
CEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PSIQSFLKSMVNILLLIMLILAVIGKLGIELTGFAALLASAGVAIGMALSGNLQNFAGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHEEEEECCCHHHHCCCE
IILLFRPYKVGDFIEASTGASGTVKEIQIFHTILITADNKMIYVPNGAMSSGVITNYSKL
EEEEECCCCCCCCEECCCCCCCCHHHHHEEEEEEEEECCEEEEECCCCCCCCCCCCHHHC
DTRRIEWNFGVDYGEDYNKVEKVLREIIANDKRILTSPAPFIELGELAASSVNIKIRVWV
CCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCEEEEEEEEE
NSSDYWNVFFSMNQTVYTIFNKEGINFPFPQLTVHQA
CCCCEEEEEEECCCEEEEEEECCCCCCCCCCCEEECC
>Mature Secondary Structure
MLLASETSIQLTNALEKLIDSGIQLGERVLGALIIFIIGKFLVNWLNRLFARILEKRKVD
CEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PSIQSFLKSMVNILLLIMLILAVIGKLGIELTGFAALLASAGVAIGMALSGNLQNFAGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHEEEEECCCHHHHCCCE
IILLFRPYKVGDFIEASTGASGTVKEIQIFHTILITADNKMIYVPNGAMSSGVITNYSKL
EEEEECCCCCCCCEECCCCCCCCHHHHHEEEEEEEEECCEEEEECCCCCCCCCCCCHHHC
DTRRIEWNFGVDYGEDYNKVEKVLREIIANDKRILTSPAPFIELGELAASSVNIKIRVWV
CCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCEEEEEEEEE
NSSDYWNVFFSMNQTVYTIFNKEGINFPFPQLTVHQA
CCCCEEEEEEECCCEEEEEEECCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]