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The map label for this gene is purB [H]

Identifier: 150004185

GI number: 150004185

Start: 2112133

End: 2113479

Strand: Direct

Name: purB [H]

Synonym: BVU_1626

Alternate gene names: 150004185

Gene position: 2112133-2113479 (Clockwise)

Preceding gene: 150004184

Following gene: 150004186

Centisome position: 40.91

GC content: 42.69

Gene sequence:

>1347_bases
ATGGAGCTCGATTTACTCACGGCAATATCACCTATTGACGGTCGATACAGAGGAAAAGCGGGAGCTTTGGCCTCTTATTT
TTCTGAATTTGCACTCATCAAGTACCGTGTGCAGGTAGAAATTGAATACTTTATCACTCTGTGTGAACTACCGTTGCCAC
AGCTGAAAAGCTTTGATCATGCACGTTTTGATGCATTGCGCGCTATTTATAAGAATTTTTCAGAAGCTGACGCACAGCGT
ATTAAAGAGATAGAAAGTGTAACAAACCACGATGTAAAGGCTGTAGAATATTTCATCAAAGAAGAATTCGATAAGTTGGG
CGGAATGGAGGAATACAAGGAATTCATTCATTTCGGATTGACATCACAGGACATAAACAATACTTCTGTACCTTTGTCTA
TAAAGGAGGCATTGGAACAAGTGTACTATCCGCAAATTGAAGAGCTGATTGCTCAGTTGACTACATACGCTGAAGAATGG
GCAAATATCCCTATGTTGGCAAAAACACATGGGCAGCCTGCTTCTCCTACTCGCCTGGGTAAAGAAATCATGGTTTTCGT
TTATCGTCTGAATCGTCAGCTGGCTGTATTGAAAGCTTGTCCTGTCACGGCTAAATTCGGTGGTGCGACGGGTAATTACA
ATGCACATCATGTAGCTTATCCCGAATATGACTGGAAAGCATTCGGAAATCAGTTCGTGGCAGAGAAGCTGGGGTTGGAA
CGTGAGGAATATACTACTCAGATTTCTAATTATGATAATCTGGGAGCCATCTTTGATGCCATGAAGCGCATCAATACCAT
TATGATTGACATGAACCGCGACTTTTGGCAGTACATCTCTATGGAGTACTTCAAACAGAAGATTAAAGCGGGCGAAGTCG
GTTCCAGCGCTATGCCGCACAAAGTAAACCCCATCGACTTCGAAAATGCTGAAGGTAATCTAGGTATGGCAAACGCTATT
TTGGAGCACCTTTCTTCTAAGTTGCCTGTTTCCCGCTTGCAACGTGACTTGACAGACTCTACTGTGTTGCGTAACGTCGG
TGTACCTTTCGGTCACATTGTGATTGCTATCCAAAGTTCGTTGAAAGGTCTGCGTAAACTGTTGCTGAATGAGAAAGCAA
TCTATGCCGACTTGGATAATTGTTGGAGTGTGGTGGCGGAAGCTATCCAGACTATTTTGCGCCGCGAAGCCTATCCTCAT
CCGTATGAGGCATTGAAAGCTTTGACCCGTACAAACCAAGCTATCACAGAAGGGGCTATTAAAGAATTCATAGAAACACT
AAATGTAAGCGAAGATATTAAAAAGGAACTGCGAGTGATTACACCGCATAATTATACAGGTATTTAA

Upstream 100 bases:

>100_bases
GAAATAAATCTTCATTTTCGCAAACTTTATGCATCTTCAGGCCGTTAAACTCAAGAAAAAGAATTAATTTTGCAGTCTGA
TAATTATCAAAGAATATAAT

Downstream 100 bases:

>100_bases
AAAATAGTTAGTTGGATTTTAATCAAAAAAGCCCGAGAGGGCGTTATACATTAATTAATAAACAAAAGGAAAACCATGAG
TACAGAAAATGAAAGCAGAC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MELDLLTAISPIDGRYRGKAGALASYFSEFALIKYRVQVEIEYFITLCELPLPQLKSFDHARFDALRAIYKNFSEADAQR
IKEIESVTNHDVKAVEYFIKEEFDKLGGMEEYKEFIHFGLTSQDINNTSVPLSIKEALEQVYYPQIEELIAQLTTYAEEW
ANIPMLAKTHGQPASPTRLGKEIMVFVYRLNRQLAVLKACPVTAKFGGATGNYNAHHVAYPEYDWKAFGNQFVAEKLGLE
REEYTTQISNYDNLGAIFDAMKRINTIMIDMNRDFWQYISMEYFKQKIKAGEVGSSAMPHKVNPIDFENAEGNLGMANAI
LEHLSSKLPVSRLQRDLTDSTVLRNVGVPFGHIVIAIQSSLKGLRKLLLNEKAIYADLDNCWSVVAEAIQTILRREAYPH
PYEALKALTRTNQAITEGAIKEFIETLNVSEDIKKELRVITPHNYTGI

Sequences:

>Translated_448_residues
MELDLLTAISPIDGRYRGKAGALASYFSEFALIKYRVQVEIEYFITLCELPLPQLKSFDHARFDALRAIYKNFSEADAQR
IKEIESVTNHDVKAVEYFIKEEFDKLGGMEEYKEFIHFGLTSQDINNTSVPLSIKEALEQVYYPQIEELIAQLTTYAEEW
ANIPMLAKTHGQPASPTRLGKEIMVFVYRLNRQLAVLKACPVTAKFGGATGNYNAHHVAYPEYDWKAFGNQFVAEKLGLE
REEYTTQISNYDNLGAIFDAMKRINTIMIDMNRDFWQYISMEYFKQKIKAGEVGSSAMPHKVNPIDFENAEGNLGMANAI
LEHLSSKLPVSRLQRDLTDSTVLRNVGVPFGHIVIAIQSSLKGLRKLLLNEKAIYADLDNCWSVVAEAIQTILRREAYPH
PYEALKALTRTNQAITEGAIKEFIETLNVSEDIKKELRVITPHNYTGI
>Mature_448_residues
MELDLLTAISPIDGRYRGKAGALASYFSEFALIKYRVQVEIEYFITLCELPLPQLKSFDHARFDALRAIYKNFSEADAQR
IKEIESVTNHDVKAVEYFIKEEFDKLGGMEEYKEFIHFGLTSQDINNTSVPLSIKEALEQVYYPQIEELIAQLTTYAEEW
ANIPMLAKTHGQPASPTRLGKEIMVFVYRLNRQLAVLKACPVTAKFGGATGNYNAHHVAYPEYDWKAFGNQFVAEKLGLE
REEYTTQISNYDNLGAIFDAMKRINTIMIDMNRDFWQYISMEYFKQKIKAGEVGSSAMPHKVNPIDFENAEGNLGMANAI
LEHLSSKLPVSRLQRDLTDSTVLRNVGVPFGHIVIAIQSSLKGLRKLLLNEKAIYADLDNCWSVVAEAIQTILRREAYPH
PYEALKALTRTNQAITEGAIKEFIETLNVSEDIKKELRVITPHNYTGI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=290, Percent_Identity=24.1379310344828, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI183227688, Length=290, Percent_Identity=24.1379310344828, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1787376, Length=448, Percent_Identity=50.6696428571429, Blast_Score=453, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24647570, Length=265, Percent_Identity=25.2830188679245, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50930; Mature: 50930

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELDLLTAISPIDGRYRGKAGALASYFSEFALIKYRVQVEIEYFITLCELPLPQLKSFDH
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCHHHHCCCH
ARFDALRAIYKNFSEADAQRIKEIESVTNHDVKAVEYFIKEEFDKLGGMEEYKEFIHFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
TSQDINNTSVPLSIKEALEQVYYPQIEELIAQLTTYAEEWANIPMLAKTHGQPASPTRLG
CCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHH
KEIMVFVYRLNRQLAVLKACPVTAKFGGATGNYNAHHVAYPEYDWKAFGNQFVAEKLGLE
HHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCC
REEYTTQISNYDNLGAIFDAMKRINTIMIDMNRDFWQYISMEYFKQKIKAGEVGSSAMPH
HHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KVNPIDFENAEGNLGMANAILEHLSSKLPVSRLQRDLTDSTVLRNVGVPFGHIVIAIQSS
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKGLRKLLLNEKAIYADLDNCWSVVAEAIQTILRREAYPHPYEALKALTRTNQAITEGAI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KEFIETLNVSEDIKKELRVITPHNYTGI
HHHHHHHCCHHHHHHHHEEECCCCCCCC
>Mature Secondary Structure
MELDLLTAISPIDGRYRGKAGALASYFSEFALIKYRVQVEIEYFITLCELPLPQLKSFDH
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCHHHHCCCH
ARFDALRAIYKNFSEADAQRIKEIESVTNHDVKAVEYFIKEEFDKLGGMEEYKEFIHFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
TSQDINNTSVPLSIKEALEQVYYPQIEELIAQLTTYAEEWANIPMLAKTHGQPASPTRLG
CCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHH
KEIMVFVYRLNRQLAVLKACPVTAKFGGATGNYNAHHVAYPEYDWKAFGNQFVAEKLGLE
HHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCC
REEYTTQISNYDNLGAIFDAMKRINTIMIDMNRDFWQYISMEYFKQKIKAGEVGSSAMPH
HHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KVNPIDFENAEGNLGMANAILEHLSSKLPVSRLQRDLTDSTVLRNVGVPFGHIVIAIQSS
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKGLRKLLLNEKAIYADLDNCWSVVAEAIQTILRREAYPHPYEALKALTRTNQAITEGAI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KEFIETLNVSEDIKKELRVITPHNYTGI
HHHHHHHCCHHHHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]