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The map label for this gene is rluB [H]

Identifier: 150004186

GI number: 150004186

Start: 2113555

End: 2115021

Strand: Direct

Name: rluB [H]

Synonym: BVU_1627

Alternate gene names: 150004186

Gene position: 2113555-2115021 (Clockwise)

Preceding gene: 150004185

Following gene: 150004187

Centisome position: 40.94

GC content: 45.6

Gene sequence:

>1467_bases
ATGAGTACAGAAAATGAAAGCAGACAAGAAGGTGCTGCAAACGAAGAAAACCAGAATGTAAGCCGTGATGGTGGTTATAA
GTCTTATAACAGAGACAGTAATTACAACAGATACAACAACGACGGCGAACAGCGTCCTTATCGTCCTCGTACGAACAGCT
ATAATCGCGAAGGTGGCGACCGTCCTTACCGTTCTTCTTATAACAACGGTGACCGTCCTTCCTATAATCGCTATAATAAT
AATGGTGACCGTCCGCAACGTCCCCGCTTTAATCCTAACAGTGAGGATGGAGGAGAACAACGTTCATACCGTCCTCGTAC
AAATTATAATCGTGAAGGTGGTGAGCAGCGTCCATATACTCCGCGCCCTCGTTTCAATAATGGTGAGGAAGGTGGTGACC
GTCAATACCGTCCCCGTACAAACTATAATCGCGAAGGTGGTGAGCAGCGTCCTTATACTCCGCGTCCCCGCACCGGAGGA
TATAATCGCGAAGGTGGCGAGCAGCGTCCATATACTCCGCGTCCCCGTTTTAACAGCGGTGAAGGTGGTGAACAGCGTTC
TTACACTCCGCGTCCTCGTACCGGCGGTTATAATCAAGGCGGCGACCGTCCTTATCGTCCTCGTACCGGTGGTTATCAAG
GTGGTGGATACAATCGTCCCTATCGCCCCCGTACGGCTGATTATAACCCGAATGCAAAGTATAGTCTGAAGAAACAGATT
GAATATAAAGATATATTGACCGATCCAAATGAGCCGATTCGTCTGAACAAGTTCTTGGCTAATGCAGGTATATGTTCCCG
TCGTGAGGCTGATGAGTTTATTACAGCAGGTGTAGTCAGTGTAAATGGTGAAGTGGTAACCGAATTAGGTACTAAGATCA
AGCGTACTGACGAAGTGAAGTTCCATGACGAACCGGTAAGTATTGAACGCAAAACTTATATTTTGTTGAATAAACCGAAG
GATTGTGTGACTACTTCTGATGATCCTCAGGAACGTAAGACTGTAATGGATTTTGTGAAAGGTGCTTGTAAGGAGCGTAT
TTATCCAGTTGGGCGTTTGGATCGTAATACTACCGGTGTATTACTGTTAACTAACGATGGTGATTTGGCTTCCAAGCTGA
CTCATCCTAAGTATTTGAAGAAGAAGATTTATCATGTATATTGTGATAAGAATGTAACCAAGGCAGATCTTGACCAGATT
GCAGCAGGTGTGACATTGGATGATGGAGAGATCCATGCAGATGCCATCAGCTATGCATCGGAAACAGACAAGTCACAAGT
CGGTATTGAAATCCATTCCGGAAAGAACCGTATTGTGCGTCGTATTTTTGAATCCTTGGGATATAAGGTAATCAAGCTGG
ACCGCGTTTATTTTGCTGGCCTGACTAAAAAAGGTTTGCGTCGCGGTGACTGGCGTTACCTGACGGAACAAGAAGTGAAC
ATGCTCCGCATGGGCTCATTTGAATAA

Upstream 100 bases:

>100_bases
ACCGCATAATTATACAGGTATTTAAAAAATAGTTAGTTGGATTTTAATCAAAAAAGCCCGAGAGGGCGTTATACATTAAT
TAATAAACAAAAGGAAAACC

Downstream 100 bases:

>100_bases
TAAACAATAAAGAGACAAAACAATGGAAAAAATTAGTAGAACAAAGATTGTTGACTTGCTGAAGCGCCGTGATTTTGGTG
CGATGGTCAATGTAAAAGGT

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSTENESRQEGAANEENQNVSRDGGYKSYNRDSNYNRYNNDGEQRPYRPRTNSYNREGGDRPYRSSYNNGDRPSYNRYNN
NGDRPQRPRFNPNSEDGGEQRSYRPRTNYNREGGEQRPYTPRPRFNNGEEGGDRQYRPRTNYNREGGEQRPYTPRPRTGG
YNREGGEQRPYTPRPRFNSGEGGEQRSYTPRPRTGGYNQGGDRPYRPRTGGYQGGGYNRPYRPRTADYNPNAKYSLKKQI
EYKDILTDPNEPIRLNKFLANAGICSRREADEFITAGVVSVNGEVVTELGTKIKRTDEVKFHDEPVSIERKTYILLNKPK
DCVTTSDDPQERKTVMDFVKGACKERIYPVGRLDRNTTGVLLLTNDGDLASKLTHPKYLKKKIYHVYCDKNVTKADLDQI
AAGVTLDDGEIHADAISYASETDKSQVGIEIHSGKNRIVRRIFESLGYKVIKLDRVYFAGLTKKGLRRGDWRYLTEQEVN
MLRMGSFE

Sequences:

>Translated_488_residues
MSTENESRQEGAANEENQNVSRDGGYKSYNRDSNYNRYNNDGEQRPYRPRTNSYNREGGDRPYRSSYNNGDRPSYNRYNN
NGDRPQRPRFNPNSEDGGEQRSYRPRTNYNREGGEQRPYTPRPRFNNGEEGGDRQYRPRTNYNREGGEQRPYTPRPRTGG
YNREGGEQRPYTPRPRFNSGEGGEQRSYTPRPRTGGYNQGGDRPYRPRTGGYQGGGYNRPYRPRTADYNPNAKYSLKKQI
EYKDILTDPNEPIRLNKFLANAGICSRREADEFITAGVVSVNGEVVTELGTKIKRTDEVKFHDEPVSIERKTYILLNKPK
DCVTTSDDPQERKTVMDFVKGACKERIYPVGRLDRNTTGVLLLTNDGDLASKLTHPKYLKKKIYHVYCDKNVTKADLDQI
AAGVTLDDGEIHADAISYASETDKSQVGIEIHSGKNRIVRRIFESLGYKVIKLDRVYFAGLTKKGLRRGDWRYLTEQEVN
MLRMGSFE
>Mature_487_residues
STENESRQEGAANEENQNVSRDGGYKSYNRDSNYNRYNNDGEQRPYRPRTNSYNREGGDRPYRSSYNNGDRPSYNRYNNN
GDRPQRPRFNPNSEDGGEQRSYRPRTNYNREGGEQRPYTPRPRFNNGEEGGDRQYRPRTNYNREGGEQRPYTPRPRTGGY
NREGGEQRPYTPRPRFNSGEGGEQRSYTPRPRTGGYNQGGDRPYRPRTGGYQGGGYNRPYRPRTADYNPNAKYSLKKQIE
YKDILTDPNEPIRLNKFLANAGICSRREADEFITAGVVSVNGEVVTELGTKIKRTDEVKFHDEPVSIERKTYILLNKPKD
CVTTSDDPQERKTVMDFVKGACKERIYPVGRLDRNTTGVLLLTNDGDLASKLTHPKYLKKKIYHVYCDKNVTKADLDQIA
AGVTLDDGEIHADAISYASETDKSQVGIEIHSGKNRIVRRIFESLGYKVIKLDRVYFAGLTKKGLRRGDWRYLTEQEVNM
LRMGSFE

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=235, Percent_Identity=36.1702127659575, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787524, Length=239, Percent_Identity=36.8200836820084, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1788510, Length=229, Percent_Identity=31.4410480349345, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87081838, Length=179, Percent_Identity=31.2849162011173, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 56166; Mature: 56035

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCC
>Mature Secondary Structure 
STENESRQEGAANEENQNVSRDGGYKSYNRDSNYNRYNNDGEQRPYRPRTNSYNREGGD
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPYRSSYNNGDRPSYNRYNNNGDRPQRPRFNPNSEDGGEQRSYRPRTNYNREGGEQRPYT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PRPRFNNGEEGGDRQYRPRTNYNREGGEQRPYTPRPRTGGYNREGGEQRPYTPRPRFNSG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EGGEQRSYTPRPRTGGYNQGGDRPYRPRTGGYQGGGYNRPYRPRTADYNPNAKYSLKKQI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHC
EYKDILTDPNEPIRLNKFLANAGICSRREADEFITAGVVSVNGEVVTELGTKIKRTDEVK
CHHHHHCCCCCCEEHHHHHHHCCCCCCHHHHHHHHHCEEEECCHHHHHHHHHHHCCCCCE
FHDEPVSIERKTYILLNKPKDCVTTSDDPQERKTVMDFVKGACKERIYPVGRLDRNTTGV
ECCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
LLLTNDGDLASKLTHPKYLKKKIYHVYCDKNVTKADLDQIAAGVTLDDGEIHADAISYAS
EEEECCCHHHHHHCCHHHHHHHHEEEEECCCCCHHHHHHHHHCCEECCCCCHHHHHHHHH
ETDKSQVGIEIHSGKNRIVRRIFESLGYKVIKLDRVYFAGLTKKGLRRGDWRYLTEQEVN
HCCCCCEEEEEECCHHHHHHHHHHHCCCEEEEECHHHHHCCCHHHCCCCCCEEECHHHHH
MLRMGSFE
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]