Click here to switch to the map view.

The map label for this gene is murE

Identifier: 150003967

GI number: 150003967

Start: 1874886

End: 1876340

Strand: Reverse

Name: murE

Synonym: BVU_1401

Alternate gene names: 150003967

Gene position: 1876340-1874886 (Counterclockwise)

Preceding gene: 150003968

Following gene: 150003966

Centisome position: 36.34

GC content: 43.92

Gene sequence:

>1455_bases
ATGAAACTGGAAAAGATTATTAAAGGAATAACAGTCAATGAAATCATAGGTGATGCATCCCAAGAAATTTCGGGAATAAA
CATGGACTCGCGCCTGATAGAGCCTGGTCATATTTTCGTTGCTGTAAAAGGAACGCAGACTGACGGACACACTTATATTC
AAAAAGCAATAGAAAAAGGAGCCCGTACAGTGGTATGTGAGAATCTTCCTGAGACATTGATTGAGAATGTTACCTACATC
AAAGTGAACGATACGGAAGACGTTGTCGGGAAACTGGCAACTACGTTTTACGGCGACCCCACCTCGAAATTGGAACTGGT
CGGTGTTACAGGCACCAACGGAAAAACAACTATTGCCACCTTATTATATAATATGTTCCGTAAATTCGGATATAAAACAG
GACTCATTTCCACTGTATGCAATTATATAGATGAGGAAGCCATTCCAACTGACCATACCACCCCTGACCCTATTACACTA
AACAAACTGCTGGGGCGCATGGCAGATGAAGGATGCAAATATGCCTTTATGGAAGTAAGCTCACATTCTGTAGCACAAAA
ACGGATCGGTGGTTTAAAATTCGCAGGCGGTATATTTACCAACCTTACCCGTGACCATCTGGACTACCATAAAACTGTAG
AGAACTATCTGAAAGCCAAAAAAGCATTCTTTGACGGTCTGCCTAAAACAGCCTTTGCCTTAACCAATCTGGATGACAAA
AACGGATTGGTCATGACTCAAAATACCAAAGCAAAGGTACACACCTATTCGCTGCGCAGCTTGAGTGATTTTAAAGGAAA
AGTGCTGGAAGATGGATTCGAAGGAATGCTGCTTGATATAAATAATGTGGAAGTCAATGTACAGTTCATCGGACGTTTCA
ATGCTTCCAACCTGCTGGCAGTCTATGGAGCAGCCTGCCTGCTTGGGAAAAAGACAGAAGAGGTATTGCTGGCATTGAGT
ACTCTTCGCCCCGTTGCCGGGCGTTTTGACTCACTGCGCTCACCCAAGGGATACACCGCCATTGTGGACTATGCCCATAC
TCCGGATGCCTTGGAAAATGTGCTGAATGCCATTCACGAGGTCCTGAATGGCAAAGGTCATGTAATTACGGTAGTAGGCG
CCGGCGGCAACCGCGATAAAGGCAAACGCCCCTTGATGGCACAAGAAGCGGTAAAGCAAAGCGATAAGGTCATTATCACC
TCTGACAATCCCCGTTTTGAAGAGCCGCAGGAAATCATCAATGATATGCTGGCAGGGCTGACTAAAGAGGATATGCGTAA
AGTGATAAGCATAGCCGACCGTAAAGAAGCGATCCGTACAGCTTGTATGCTGGCCCAAGCTAAAGATGTCATTTTAGTTG
CCGGGAAAGGACACGAAAATTATCAGGAAATCAAAGGTATAAAGCACCATTTCGACGATAAAGAAGTATTAAGAGATATT
TTTGCTAATGAATAA

Upstream 100 bases:

>100_bases
CATCGGTAAAGTGAAGTCACAAAGTATCCCGGCCGGCAACACTTTGCGCAAAGGACAAACCATACAATTACGATTAAATT
AACAGTGTAACAGGAAGAAT

Downstream 100 bases:

>100_bases
ACCAACCAAAAGATGTTATATTATTTATTTCAATATTTAGAAAAGTTCGATTTTCCCGGAGCCGGTATGTTCGGTTACGT
TTCATTCCGTTCATTGATGG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKLEKIIKGITVNEIIGDASQEISGINMDSRLIEPGHIFVAVKGTQTDGHTYIQKAIEKGARTVVCENLPETLIENVTYI
KVNDTEDVVGKLATTFYGDPTSKLELVGVTGTNGKTTIATLLYNMFRKFGYKTGLISTVCNYIDEEAIPTDHTTPDPITL
NKLLGRMADEGCKYAFMEVSSHSVAQKRIGGLKFAGGIFTNLTRDHLDYHKTVENYLKAKKAFFDGLPKTAFALTNLDDK
NGLVMTQNTKAKVHTYSLRSLSDFKGKVLEDGFEGMLLDINNVEVNVQFIGRFNASNLLAVYGAACLLGKKTEEVLLALS
TLRPVAGRFDSLRSPKGYTAIVDYAHTPDALENVLNAIHEVLNGKGHVITVVGAGGNRDKGKRPLMAQEAVKQSDKVIIT
SDNPRFEEPQEIINDMLAGLTKEDMRKVISIADRKEAIRTACMLAQAKDVILVAGKGHENYQEIKGIKHHFDDKEVLRDI
FANE

Sequences:

>Translated_484_residues
MKLEKIIKGITVNEIIGDASQEISGINMDSRLIEPGHIFVAVKGTQTDGHTYIQKAIEKGARTVVCENLPETLIENVTYI
KVNDTEDVVGKLATTFYGDPTSKLELVGVTGTNGKTTIATLLYNMFRKFGYKTGLISTVCNYIDEEAIPTDHTTPDPITL
NKLLGRMADEGCKYAFMEVSSHSVAQKRIGGLKFAGGIFTNLTRDHLDYHKTVENYLKAKKAFFDGLPKTAFALTNLDDK
NGLVMTQNTKAKVHTYSLRSLSDFKGKVLEDGFEGMLLDINNVEVNVQFIGRFNASNLLAVYGAACLLGKKTEEVLLALS
TLRPVAGRFDSLRSPKGYTAIVDYAHTPDALENVLNAIHEVLNGKGHVITVVGAGGNRDKGKRPLMAQEAVKQSDKVIIT
SDNPRFEEPQEIINDMLAGLTKEDMRKVISIADRKEAIRTACMLAQAKDVILVAGKGHENYQEIKGIKHHFDDKEVLRDI
FANE
>Mature_484_residues
MKLEKIIKGITVNEIIGDASQEISGINMDSRLIEPGHIFVAVKGTQTDGHTYIQKAIEKGARTVVCENLPETLIENVTYI
KVNDTEDVVGKLATTFYGDPTSKLELVGVTGTNGKTTIATLLYNMFRKFGYKTGLISTVCNYIDEEAIPTDHTTPDPITL
NKLLGRMADEGCKYAFMEVSSHSVAQKRIGGLKFAGGIFTNLTRDHLDYHKTVENYLKAKKAFFDGLPKTAFALTNLDDK
NGLVMTQNTKAKVHTYSLRSLSDFKGKVLEDGFEGMLLDINNVEVNVQFIGRFNASNLLAVYGAACLLGKKTEEVLLALS
TLRPVAGRFDSLRSPKGYTAIVDYAHTPDALENVLNAIHEVLNGKGHVITVVGAGGNRDKGKRPLMAQEAVKQSDKVIIT
SDNPRFEEPQEIINDMLAGLTKEDMRKVISIADRKEAIRTACMLAQAKDVILVAGKGHENYQEIKGIKHHFDDKEVLRDI
FANE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=479, Percent_Identity=36.9519832985386, Blast_Score=259, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_BACV8 (A6L075)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298711.1
- ProteinModelPortal:   A6L075
- SMR:   A6L075
- STRING:   A6L075
- GeneID:   5302367
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1401
- NMPDR:   fig|435590.6.peg.1323
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- ProtClustDB:   PRK00139
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 53284; Mature: 53284

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 180-180 BINDING 186-186 BINDING 188-188 BINDING 378-378 BINDING 455-455 BINDING 459-459

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEKIIKGITVNEIIGDASQEISGINMDSRLIEPGHIFVAVKGTQTDGHTYIQKAIEKG
CCHHHHHCCCCHHHHHCCHHHHHCCCCCCCCEECCCEEEEEEECCCCCCHHHHHHHHHCC
ARTVVCENLPETLIENVTYIKVNDTEDVVGKLATTFYGDPTSKLELVGVTGTNGKTTIAT
CCEEEHHCCHHHHHCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHH
LLYNMFRKFGYKTGLISTVCNYIDEEAIPTDHTTPDPITLNKLLGRMADEGCKYAFMEVS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEECC
SHSVAQKRIGGLKFAGGIFTNLTRDHLDYHKTVENYLKAKKAFFDGLPKTAFALTNLDDK
HHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCC
NGLVMTQNTKAKVHTYSLRSLSDFKGKVLEDGFEGMLLDINNVEVNVQFIGRFNASNLLA
CCEEEEECCCCEEEEEEHHHHHHHCCHHHHCCCCEEEEEECCEEEEEEEEEECCCCCHHH
VYGAACLLGKKTEEVLLALSTLRPVAGRFDSLRSPKGYTAIVDYAHTPDALENVLNAIHE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHH
VLNGKGHVITVVGAGGNRDKGKRPLMAQEAVKQSDKVIITSDNPRFEEPQEIINDMLAGL
HHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCC
TKEDMRKVISIADRKEAIRTACMLAQAKDVILVAGKGHENYQEIKGIKHHFDDKEVLRDI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHH
FANE
HCCC
>Mature Secondary Structure
MKLEKIIKGITVNEIIGDASQEISGINMDSRLIEPGHIFVAVKGTQTDGHTYIQKAIEKG
CCHHHHHCCCCHHHHHCCHHHHHCCCCCCCCEECCCEEEEEEECCCCCCHHHHHHHHHCC
ARTVVCENLPETLIENVTYIKVNDTEDVVGKLATTFYGDPTSKLELVGVTGTNGKTTIAT
CCEEEHHCCHHHHHCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHH
LLYNMFRKFGYKTGLISTVCNYIDEEAIPTDHTTPDPITLNKLLGRMADEGCKYAFMEVS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEECC
SHSVAQKRIGGLKFAGGIFTNLTRDHLDYHKTVENYLKAKKAFFDGLPKTAFALTNLDDK
HHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCC
NGLVMTQNTKAKVHTYSLRSLSDFKGKVLEDGFEGMLLDINNVEVNVQFIGRFNASNLLA
CCEEEEECCCCEEEEEEHHHHHHHCCHHHHCCCCEEEEEECCEEEEEEEEEECCCCCHHH
VYGAACLLGKKTEEVLLALSTLRPVAGRFDSLRSPKGYTAIVDYAHTPDALENVLNAIHE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHH
VLNGKGHVITVVGAGGNRDKGKRPLMAQEAVKQSDKVIITSDNPRFEEPQEIINDMLAGL
HHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCC
TKEDMRKVISIADRKEAIRTACMLAQAKDVILVAGKGHENYQEIKGIKHHFDDKEVLRDI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHH
FANE
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA