Click here to switch to the map view.

The map label for this gene is miaB

Identifier: 150003734

GI number: 150003734

Start: 1581438

End: 1582808

Strand: Direct

Name: miaB

Synonym: BVU_1165

Alternate gene names: 150003734

Gene position: 1581438-1582808 (Clockwise)

Preceding gene: 150003730

Following gene: 150003735

Centisome position: 30.63

GC content: 45.08

Gene sequence:

>1371_bases
ATGAAAGAAACAACAGGAGCAGACTTTAAATCTGCAACTGATTCCGAAAACAAGAAATTGTTTATTGAAACTTACGGATG
CCAGATGAATGTTGCTGATAGTGAAGTGATTGCTTCTATTATGCAAATGGCAGGCTACCATGTATGCGAAACATTGGATG
AAGCGGATGCTGTATTTATGAATACCTGTTCCATTCGCGACAATGCAGAACAAAAGATTCTGAACCGTCTGGAGTTTTTC
CATTCTATGAAGAAAAACAAACGCCGGAATCTGATTGTCGGTGTGTTGGGCTGCATGGCAGAACGTGTAAAAGATGATTT
GATCGAGAACCATCATGTAGATCTTGTCGTAGGACCGGATGCCTATCTCACTTTGCCCGATTTGGTCGCTTCGGTGGAAG
CGGGGGAGAAAGCCATTAATGTGGAGCTTTCTACTACAGAAACCTATCGTGAGGTAATTCCGTCCCGTATTTGCGGCAAT
CATATCTCCGGGTTTGTATCCATTATGCGTGGTTGCAATAATTTCTGTCATTATTGTATTGTGCCGTATACCCGTGGGCG
TGAGCGCAGCCGTGATGTGGAAAGTATTCTGAATGAGGTGCGTGACTTGGCAGACAAAGGTTATAAAGAAGTAACGTTGC
TGGGACAGAATGTGAATTCTTATCGTTTTGAGAAAGAGGGTGAAATAATTACTTTCCCGATGCTGCTCCGCACAGTGGCC
GAGGCGGTTCCGGACATGCGTGTTCGTTTTACCACTTCACATCCCAAGGATATGAGTGATGAAACCTTGCAAGTCATTGC
GGAAACGCCTAATGTATGCAAGCATATTCATTTACCGGTACAGAGCGGCAGTTCGCGCATATTGAAGCTGATGAACCGCA
AATATACCCGTGAGTGGTATTTGGAAAGGGTAGCGGCTATTCGCCGTATCATTCCCGATTGTGGTCTGAGTACTGATATT
TTCTCCGGCTATCATTCGGAAACGGAAGAAGACCATCAGGAATCTCTATCCTTGATGCGTGAGTGTGCGTACGATTCCGC
CTTTATGTTTAAATATTCTGAACGTCCCGGAACATATGCTTCCAAGCATCTGCCGGATGATGTACCCGAAGAGGTTAAGA
TACGCCGGCTGAATGAAATCATTGAATTGCAGAACCGTCTTTCTGCCGAAAGTAATGCCCGTGATGTAGGCAAGACTTTC
GAAGTGATGGTAGAAGGTGTTTCCAAGCGTTCCAGAGAGCAGCTTTTTGGCCGTACACAGCAAAACAAGGTGGTGGTCTT
TGATCGTGGGAATCATCGTATCGGTGATTTTGTCCATGTACGCATTACTGAAGCCAGTTCGGCTACATTGAAAGGAGAAG
AGGTGTTCTGA

Upstream 100 bases:

>100_bases
TGCATAAATCTCTTATAATATTCAATTTTCGGCTCTTTTATTCGTTTAATTTGTCGGTAACTCCGTATCTTTGCCTACGA
AACCATAAAACAGAGAAAAT

Downstream 100 bases:

>100_bases
TGGGGAGAAAAGAAGAATATAAGGAAAAGAACTTGCAATATTTGCAAGTTCTTTCTACACAGGAAGGTATTTGTCCTTTA
CCTTGTAATATGTTTTATAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKETTGADFKSATDSENKKLFIETYGCQMNVADSEVIASIMQMAGYHVCETLDEADAVFMNTCSIRDNAEQKILNRLEFF
HSMKKNKRRNLIVGVLGCMAERVKDDLIENHHVDLVVGPDAYLTLPDLVASVEAGEKAINVELSTTETYREVIPSRICGN
HISGFVSIMRGCNNFCHYCIVPYTRGRERSRDVESILNEVRDLADKGYKEVTLLGQNVNSYRFEKEGEIITFPMLLRTVA
EAVPDMRVRFTTSHPKDMSDETLQVIAETPNVCKHIHLPVQSGSSRILKLMNRKYTREWYLERVAAIRRIIPDCGLSTDI
FSGYHSETEEDHQESLSLMRECAYDSAFMFKYSERPGTYASKHLPDDVPEEVKIRRLNEIIELQNRLSAESNARDVGKTF
EVMVEGVSKRSREQLFGRTQQNKVVVFDRGNHRIGDFVHVRITEASSATLKGEEVF

Sequences:

>Translated_456_residues
MKETTGADFKSATDSENKKLFIETYGCQMNVADSEVIASIMQMAGYHVCETLDEADAVFMNTCSIRDNAEQKILNRLEFF
HSMKKNKRRNLIVGVLGCMAERVKDDLIENHHVDLVVGPDAYLTLPDLVASVEAGEKAINVELSTTETYREVIPSRICGN
HISGFVSIMRGCNNFCHYCIVPYTRGRERSRDVESILNEVRDLADKGYKEVTLLGQNVNSYRFEKEGEIITFPMLLRTVA
EAVPDMRVRFTTSHPKDMSDETLQVIAETPNVCKHIHLPVQSGSSRILKLMNRKYTREWYLERVAAIRRIIPDCGLSTDI
FSGYHSETEEDHQESLSLMRECAYDSAFMFKYSERPGTYASKHLPDDVPEEVKIRRLNEIIELQNRLSAESNARDVGKTF
EVMVEGVSKRSREQLFGRTQQNKVVVFDRGNHRIGDFVHVRITEASSATLKGEEVF
>Mature_456_residues
MKETTGADFKSATDSENKKLFIETYGCQMNVADSEVIASIMQMAGYHVCETLDEADAVFMNTCSIRDNAEQKILNRLEFF
HSMKKNKRRNLIVGVLGCMAERVKDDLIENHHVDLVVGPDAYLTLPDLVASVEAGEKAINVELSTTETYREVIPSRICGN
HISGFVSIMRGCNNFCHYCIVPYTRGRERSRDVESILNEVRDLADKGYKEVTLLGQNVNSYRFEKEGEIITFPMLLRTVA
EAVPDMRVRFTTSHPKDMSDETLQVIAETPNVCKHIHLPVQSGSSRILKLMNRKYTREWYLERVAAIRRIIPDCGLSTDI
FSGYHSETEEDHQESLSLMRECAYDSAFMFKYSERPGTYASKHLPDDVPEEVKIRRLNEIIELQNRLSAESNARDVGKTF
EVMVEGVSKRSREQLFGRTQQNKVVVFDRGNHRIGDFVHVRITEASSATLKGEEVF

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=474, Percent_Identity=47.6793248945148, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI28872782, Length=474, Percent_Identity=47.6793248945148, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI93277076, Length=451, Percent_Identity=26.1640798226164, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=39.8190045248869, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1787057, Length=461, Percent_Identity=28.6334056399132, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=44.3722943722944, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71996771, Length=296, Percent_Identity=26.3513513513513, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=44.9790794979079, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI19922432, Length=452, Percent_Identity=28.5398230088496, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_BACV8 (A6KZJ2)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298478.1
- ProteinModelPortal:   A6KZJ2
- SMR:   A6KZJ2
- STRING:   A6KZJ2
- GeneID:   5302131
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1165
- NMPDR:   fig|435590.6.peg.1104
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14329
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51935; Mature: 51935

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETTGADFKSATDSENKKLFIETYGCQMNVADSEVIASIMQMAGYHVCETLDEADAVFM
CCCCCCCCCCCCCCCCCCEEEEEEECCEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
NTCSIRDNAEQKILNRLEFFHSMKKNKRRNLIVGVLGCMAERVKDDLIENHHVDLVVGPD
EECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
AYLTLPDLVASVEAGEKAINVELSTTETYREVIPSRICGNHISGFVSIMRGCNNFCHYCI
CEEEHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VPYTRGRERSRDVESILNEVRDLADKGYKEVTLLGQNVNSYRFEKEGEIITFPMLLRTVA
ECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCEEEHHHHHHHHH
EAVPDMRVRFTTSHPKDMSDETLQVIAETPNVCKHIHLPVQSGSSRILKLMNRKYTREWY
HHCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHH
LERVAAIRRIIPDCGLSTDIFSGYHSETEEDHQESLSLMRECAYDSAFMFKYSERPGTYA
HHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
SKHLPDDVPEEVKIRRLNEIIELQNRLSAESNARDVGKTFEVMVEGVSKRSREQLFGRTQ
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QNKVVVFDRGNHRIGDFVHVRITEASSATLKGEEVF
CCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKETTGADFKSATDSENKKLFIETYGCQMNVADSEVIASIMQMAGYHVCETLDEADAVFM
CCCCCCCCCCCCCCCCCCEEEEEEECCEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
NTCSIRDNAEQKILNRLEFFHSMKKNKRRNLIVGVLGCMAERVKDDLIENHHVDLVVGPD
EECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
AYLTLPDLVASVEAGEKAINVELSTTETYREVIPSRICGNHISGFVSIMRGCNNFCHYCI
CEEEHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VPYTRGRERSRDVESILNEVRDLADKGYKEVTLLGQNVNSYRFEKEGEIITFPMLLRTVA
ECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCEEEHHHHHHHHH
EAVPDMRVRFTTSHPKDMSDETLQVIAETPNVCKHIHLPVQSGSSRILKLMNRKYTREWY
HHCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHH
LERVAAIRRIIPDCGLSTDIFSGYHSETEEDHQESLSLMRECAYDSAFMFKYSERPGTYA
HHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
SKHLPDDVPEEVKIRRLNEIIELQNRLSAESNARDVGKTFEVMVEGVSKRSREQLFGRTQ
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QNKVVVFDRGNHRIGDFVHVRITEASSATLKGEEVF
CCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA