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The map label for this gene is rluB [H]

Identifier: 148828349

GI number: 148828349

Start: 1793305

End: 1794378

Strand: Direct

Name: rluB [H]

Synonym: CGSHiGG_09715

Alternate gene names: 148828349

Gene position: 1793305-1794378 (Clockwise)

Preceding gene: 148828348

Following gene: 148828350

Centisome position: 95.03

GC content: 41.62

Gene sequence:

>1074_bases
ATGAAACCTAGTCAAAAACAAACTCAAAGACAACCGCACTTTTCCAAAGATTCAGCAAAGAAACGTGATTTTTCAGCTAA
AAATGACAGACGTTCAGTATCTCGTATTGCTTGCGTAGAAACAGCAAATACGAAAAAAAGTGCGGTTAATTCTGACAACA
AATTTTTATCTAAGCCAAAAGCGAAACCTATTGTAAAAGCCTCGAATCAACCTAAAGCAGAGGGCGAAAAATTACAGAAA
GTTTTAGCGCGTGCGGGGCAAGGCTCTCGTCGTGAAATTGAAACGATGATTGCCGCAGGCCGAGTGAGCGTAGAGGGTAA
AATTGCGACTTTAGGCGATCGTATTGACGTACATTCTGGTGTGAAAGTGCGTATTGATGGTCAAATCATTAACCTTAGCC
ACGCACAAAAAGAAATTTGCCGTGTATTAATGTATTACAAACCTGAAGGCGAACTTTGTACACGTAGCGACCCAAAAGGG
CGCGCAACCGTGTTTGATCGTTTGCCGCATTTAACGGGATCTCGTTGGATTGCGGTGGGGCGTTTAGATATTAATACATC
GGGTTTATTGCTTTTTACCACTGATGGCGAATTAGCAAATCGTTTAATGCACCCAAGCCGTGAAGTTGAACGTGAATATT
CTGTGCGTGTATTTGGTCAAGTTGATGATGCGATGTTAGCGCGTTTACGCAAAGGCGTTCAGCTTGAAGATGGTTCTGCT
AATTTTAAAGAAATTAAATTTGCAGGCGGTGTGGGTATTAACCAATGGTATGACGTAACCTTGATGGAAGGACGTAACCG
TGAGGTTCGTCGTTTGTGGGAATCCCAAGGGATTCAAGTTAGCCGCTTGATTCGAATTCGTTATGGAAATATCAAACTGA
TGAAAGGTTTGCCTCGTGGTGGCTGGGAAGAAATGGATCTTGAAAACGTCAATTATTTACGTGAATTGGTGGGATTACCT
GCTGAGACTGAAACGAAATTAGATGTAAAACAGGCTAGTCGTCGTCCAAAATCTGGGCAAATTCGTAAAGCGGTAAAACG
TTATTCGGAGATGAACAAACGTTATAAAAAATAA

Upstream 100 bases:

>100_bases
AATCACCAGTGATTTTGCGCGAAGGAAGTGGAAGTACTGCTCCTTTTATTTAACTTATAGACGCTTGTGAAAGCGACCTT
TCCATTATTCAAGGAAAACA

Downstream 100 bases:

>100_bases
GGACTTTTTTATGAAAATGCATCAATTGCGTTATATCGTTGAGATTGTAAACCAAAATTTGAATGTCACAGAAGCGGCAA
ATGCACTTTACACATCACAG

Product: methyltransferase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKPSQKQTQRQPHFSKDSAKKRDFSAKNDRRSVSRIACVETANTKKSAVNSDNKFLSKPKAKPIVKASNQPKAEGEKLQK
VLARAGQGSRREIETMIAAGRVSVEGKIATLGDRIDVHSGVKVRIDGQIINLSHAQKEICRVLMYYKPEGELCTRSDPKG
RATVFDRLPHLTGSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDDAMLARLRKGVQLEDGSA
NFKEIKFAGGVGINQWYDVTLMEGRNREVRRLWESQGIQVSRLIRIRYGNIKLMKGLPRGGWEEMDLENVNYLRELVGLP
AETETKLDVKQASRRPKSGQIRKAVKRYSEMNKRYKK

Sequences:

>Translated_357_residues
MKPSQKQTQRQPHFSKDSAKKRDFSAKNDRRSVSRIACVETANTKKSAVNSDNKFLSKPKAKPIVKASNQPKAEGEKLQK
VLARAGQGSRREIETMIAAGRVSVEGKIATLGDRIDVHSGVKVRIDGQIINLSHAQKEICRVLMYYKPEGELCTRSDPKG
RATVFDRLPHLTGSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDDAMLARLRKGVQLEDGSA
NFKEIKFAGGVGINQWYDVTLMEGRNREVRRLWESQGIQVSRLIRIRYGNIKLMKGLPRGGWEEMDLENVNYLRELVGLP
AETETKLDVKQASRRPKSGQIRKAVKRYSEMNKRYKK
>Mature_357_residues
MKPSQKQTQRQPHFSKDSAKKRDFSAKNDRRSVSRIACVETANTKKSAVNSDNKFLSKPKAKPIVKASNQPKAEGEKLQK
VLARAGQGSRREIETMIAAGRVSVEGKIATLGDRIDVHSGVKVRIDGQIINLSHAQKEICRVLMYYKPEGELCTRSDPKG
RATVFDRLPHLTGSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDDAMLARLRKGVQLEDGSA
NFKEIKFAGGVGINQWYDVTLMEGRNREVRRLWESQGIQVSRLIRIRYGNIKLMKGLPRGGWEEMDLENVNYLRELVGLP
AETETKLDVKQASRRPKSGQIRKAVKRYSEMNKRYKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=277, Percent_Identity=73.2851985559567, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1790453, Length=245, Percent_Identity=29.7959183673469, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=30.5084745762712, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081838, Length=190, Percent_Identity=32.1052631578947, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 40462; Mature: 40462

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSQKQTQRQPHFSKDSAKKRDFSAKNDRRSVSRIACVETANTKKSAVNSDNKFLSKPK
CCCCHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHEEECCCCHHHHCCCCCHHHCCCC
AKPIVKASNQPKAEGEKLQKVLARAGQGSRREIETMIAAGRVSVEGKIATLGDRIDVHSG
CCCEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCEEEECCCEEECCCC
VKVRIDGQIINLSHAQKEICRVLMYYKPEGELCTRSDPKGRATVFDRLPHLTGSRWIAVG
CEEEECCEEEECHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEE
RLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDDAMLARLRKGVQLEDGSA
EEEECCCCEEEEECCHHHHHHHCCCCHHHHHCCCEEEECCCCHHHHHHHHCCCEECCCCC
NFKEIKFAGGVGINQWYDVTLMEGRNREVRRLWESQGIQVSRLIRIRYGNIKLMKGLPRG
CCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEEEECCEEEEECCCCC
GWEEMDLENVNYLRELVGLPAETETKLDVKQASRRPKSGQIRKAVKRYSEMNKRYKK
CCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPSQKQTQRQPHFSKDSAKKRDFSAKNDRRSVSRIACVETANTKKSAVNSDNKFLSKPK
CCCCHHHHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHEEECCCCHHHHCCCCCHHHCCCC
AKPIVKASNQPKAEGEKLQKVLARAGQGSRREIETMIAAGRVSVEGKIATLGDRIDVHSG
CCCEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCEEEECCCEEECCCC
VKVRIDGQIINLSHAQKEICRVLMYYKPEGELCTRSDPKGRATVFDRLPHLTGSRWIAVG
CEEEECCEEEECHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEE
RLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDDAMLARLRKGVQLEDGSA
EEEECCCCEEEEECCHHHHHHHCCCCHHHHHCCCEEEECCCCHHHHHHHHCCCEECCCCC
NFKEIKFAGGVGINQWYDVTLMEGRNREVRRLWESQGIQVSRLIRIRYGNIKLMKGLPRG
CCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEEEECCEEEEECCCCC
GWEEMDLENVNYLRELVGLPAETETKLDVKQASRRPKSGQIRKAVKRYSEMNKRYKK
CCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]