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The map label for this gene is cysB [H]

Identifier: 148828350

GI number: 148828350

Start: 1794389

End: 1795360

Strand: Direct

Name: cysB [H]

Synonym: CGSHiGG_09720

Alternate gene names: 148828350

Gene position: 1794389-1795360 (Clockwise)

Preceding gene: 148828349

Following gene: 148828352

Centisome position: 95.08

GC content: 39.09

Gene sequence:

>972_bases
ATGAAAATGCATCAATTGCGTTATATCGTTGAGATTGTAAACCAAAATTTGAATGTCACAGAAGCGGCAAATGCACTTTA
CACATCACAGCCGGGTATCAGTAAACAAGTTCGCCTATTAGAAGATGAATTAGGTCTGGAAATTTTTGAGCGTCATGGCA
AGCATATAAAATCAATTACACCAGCAGGGAAAAAGATTATCTCAATTGCACGCGAGTTACTTGTTAAAGCGCAAGGTATC
CGTGCCGTTGCCGATGAATATACTCGGCCAAATCACGGTGTATTACGTATTGCGACGACAAATACTCAGGCACGTTATAT
GCTGCCAAGCGTGATTGAACGTTTTTCTAAAAAATATCCTGATGTGAGCTTGCATATTCATCAAGGTTCGCCAACACAAA
TTCACGATGCTTTAATGTCAGGTGAAGTGGATTTGGCAATTACCACTGAAGCACCTTATTTATTTGATGATTTAGTTCAG
ATTCCATGCTATTGGTGGAATCGTGCTGTGATTGTTTCGCCAGAACATCCGCTTGCCAAAGTGAAAGAATTGACCATTGA
GGAATTAGGCAAATACCCACTTGTGACTTACACGTTTGGTTTTACGGGTGTTTCTGATTTAGATTCCGCATTTAATAGTG
CAGGCATTTTACCAAATATAGTTTTTACGGCTACCGATGCTGATGTTATTAAAACTTATGTGCGCTTGGGTTTAGGGGTG
GGCATTATGGCATCTATGGCACATACGGCATTAGATGCGGATCTTGTGCTAATTGATGCAAGCCATTTGTTCCGCCCAAG
TATGACGAATATTGCATTTAAACATAGCACGTTCTTACGTAATTATATGTACGATTTTATGGAATATTTTTCACCGCACT
TAACTCGTAGTGTCGTAGAAAAAGCCGAGCGTTTGCGTGATAACAATTCAGTGAAAAAATTATTTGAAGGCATTGAATTG
GAAACAAAATAA

Upstream 100 bases:

>100_bases
GTAAAACAGGCTAGTCGTCGTCCAAAATCTGGGCAAATTCGTAAAGCGGTAAAACGTTATTCGGAGATGAACAAACGTTA
TAAAAAATAAGGACTTTTTT

Downstream 100 bases:

>100_bases
CAATGTATAAATGAAGTGCGGTTGAAATTTAATAAGAATTTTAACCGCACTTGAAAGATTAAAACAGATCGTGATATTTC
ACTAAATCTTTACGCTGAAT

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKMHQLRYIVEIVNQNLNVTEAANALYTSQPGISKQVRLLEDELGLEIFERHGKHIKSITPAGKKIISIARELLVKAQGI
RAVADEYTRPNHGVLRIATTNTQARYMLPSVIERFSKKYPDVSLHIHQGSPTQIHDALMSGEVDLAITTEAPYLFDDLVQ
IPCYWWNRAVIVSPEHPLAKVKELTIEELGKYPLVTYTFGFTGVSDLDSAFNSAGILPNIVFTATDADVIKTYVRLGLGV
GIMASMAHTALDADLVLIDASHLFRPSMTNIAFKHSTFLRNYMYDFMEYFSPHLTRSVVEKAERLRDNNSVKKLFEGIEL
ETK

Sequences:

>Translated_323_residues
MKMHQLRYIVEIVNQNLNVTEAANALYTSQPGISKQVRLLEDELGLEIFERHGKHIKSITPAGKKIISIARELLVKAQGI
RAVADEYTRPNHGVLRIATTNTQARYMLPSVIERFSKKYPDVSLHIHQGSPTQIHDALMSGEVDLAITTEAPYLFDDLVQ
IPCYWWNRAVIVSPEHPLAKVKELTIEELGKYPLVTYTFGFTGVSDLDSAFNSAGILPNIVFTATDADVIKTYVRLGLGV
GIMASMAHTALDADLVLIDASHLFRPSMTNIAFKHSTFLRNYMYDFMEYFSPHLTRSVVEKAERLRDNNSVKKLFEGIEL
ETK
>Mature_323_residues
MKMHQLRYIVEIVNQNLNVTEAANALYTSQPGISKQVRLLEDELGLEIFERHGKHIKSITPAGKKIISIARELLVKAQGI
RAVADEYTRPNHGVLRIATTNTQARYMLPSVIERFSKKYPDVSLHIHQGSPTQIHDALMSGEVDLAITTEAPYLFDDLVQ
IPCYWWNRAVIVSPEHPLAKVKELTIEELGKYPLVTYTFGFTGVSDLDSAFNSAGILPNIVFTATDADVIKTYVRLGLGV
GIMASMAHTALDADLVLIDASHLFRPSMTNIAFKHSTFLRNYMYDFMEYFSPHLTRSVVEKAERLRDNNSVKKLFEGIEL
ETK

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=324, Percent_Identity=63.2716049382716, Blast_Score=430, Evalue=1e-122,
Organism=Escherichia coli, GI1788296, Length=309, Percent_Identity=41.747572815534, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI157672245, Length=226, Percent_Identity=32.3008849557522, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790399, Length=260, Percent_Identity=26.5384615384615, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788887, Length=281, Percent_Identity=27.4021352313167, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788481, Length=294, Percent_Identity=25.1700680272109, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787601, Length=187, Percent_Identity=30.4812834224599, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI2367136, Length=242, Percent_Identity=26.0330578512397, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790262, Length=204, Percent_Identity=25.9803921568627, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI145693105, Length=188, Percent_Identity=25, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789204, Length=239, Percent_Identity=25.5230125523013, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788626, Length=214, Percent_Identity=26.6355140186916, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081904, Length=220, Percent_Identity=26.3636363636364, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36379; Mature: 36379

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMHQLRYIVEIVNQNLNVTEAANALYTSQPGISKQVRLLEDELGLEIFERHGKHIKSIT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCC
PAGKKIISIARELLVKAQGIRAVADEYTRPNHGVLRIATTNTQARYMLPSVIERFSKKYP
CCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
DVSLHIHQGSPTQIHDALMSGEVDLAITTEAPYLFDDLVQIPCYWWNRAVIVSPEHPLAK
CEEEEEECCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEECCCEEEECCCCCHHH
VKELTIEELGKYPLVTYTFGFTGVSDLDSAFNSAGILPNIVFTATDADVIKTYVRLGLGV
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCH
GIMASMAHTALDADLVLIDASHLFRPSMTNIAFKHSTFLRNYMYDFMEYFSPHLTRSVVE
HHHHHHHHHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
KAERLRDNNSVKKLFEGIELETK
HHHHHCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKMHQLRYIVEIVNQNLNVTEAANALYTSQPGISKQVRLLEDELGLEIFERHGKHIKSIT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCC
PAGKKIISIARELLVKAQGIRAVADEYTRPNHGVLRIATTNTQARYMLPSVIERFSKKYP
CCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
DVSLHIHQGSPTQIHDALMSGEVDLAITTEAPYLFDDLVQIPCYWWNRAVIVSPEHPLAK
CEEEEEECCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEECCCEEEECCCCCHHH
VKELTIEELGKYPLVTYTFGFTGVSDLDSAFNSAGILPNIVFTATDADVIKTYVRLGLGV
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCH
GIMASMAHTALDADLVLIDASHLFRPSMTNIAFKHSTFLRNYMYDFMEYFSPHLTRSVVE
HHHHHHHHHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
KAERLRDNNSVKKLFEGIELETK
HHHHHCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]