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The map label for this gene is dut

Identifier: 148828131

GI number: 148828131

Start: 1544440

End: 1544895

Strand: Direct

Name: dut

Synonym: CGSHiGG_08345

Alternate gene names: 148828131

Gene position: 1544440-1544895 (Clockwise)

Preceding gene: 148828130

Following gene: 148828132

Centisome position: 81.84

GC content: 40.35

Gene sequence:

>456_bases
ATGAAAAAAATTGACGTAAAAATTTTAGATTCTCGCATTGGAAATGAATTTCCTCTGCCAACTTACGCAACTGAAGGTTC
AGCAGGTCTTGATTTACGTGCGTTGATCGATGAAAGCTTTGAAATTCAACCAGGCGAAACCAAATTAATTCCGACAGGGC
TATCAATTTATATTGCGGATCCCAATTTAGCTGCCGTGATTTTGCCTCGCTCGGGTCTTGGCCATAAACACGGCATTGTG
TTAGGAAACCTTGTGGGATTAATTGACTCCGATTATCAAGGGCCATTAATGGTATCAATGTGGAACCGTGGAAATGAACC
ATTCAAAATTGAAGTCGGCGATCGTATTGCTCAACTTGTTTTCGTGCCAGTAGTACAAGCTGAATTTAATATTGTAGAAG
ATTTTCAACAAACCGAACGTGGAGAAGGCGGTTTCGGTCATTCAGGTAAACAATAA

Upstream 100 bases:

>100_bases
TTGCCGCTGATTTAGTCAATGAAATCATCGAACGCTATCAAAAAACCTTATAATTCCAACCGCACTTTTACCTAAAAATG
AATTTCAAACGGAGTATTAA

Downstream 100 bases:

>100_bases
TTTATGGTAGAAGAACAATTATCTTTATCAGGCGTAGAAGAAATTGCGCCTAAAATTGAAACACCGAAAATTGAAAAAAG
AACGGTAAAAGAACGTCGTC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MKKIDVKILDSRIGNEFPLPTYATEGSAGLDLRALIDESFEIQPGETKLIPTGLSIYIADPNLAAVILPRSGLGHKHGIV
LGNLVGLIDSDYQGPLMVSMWNRGNEPFKIEVGDRIAQLVFVPVVQAEFNIVEDFQQTERGEGGFGHSGKQ

Sequences:

>Translated_151_residues
MKKIDVKILDSRIGNEFPLPTYATEGSAGLDLRALIDESFEIQPGETKLIPTGLSIYIADPNLAAVILPRSGLGHKHGIV
LGNLVGLIDSDYQGPLMVSMWNRGNEPFKIEVGDRIAQLVFVPVVQAEFNIVEDFQQTERGEGGFGHSGKQ
>Mature_151_residues
MKKIDVKILDSRIGNEFPLPTYATEGSAGLDLRALIDESFEIQPGETKLIPTGLSIYIADPNLAAVILPRSGLGHKHGIV
LGNLVGLIDSDYQGPLMVSMWNRGNEPFKIEVGDRIAQLVFVPVVQAEFNIVEDFQQTERGEGGFGHSGKQ

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=142, Percent_Identity=38.7323943661972, Blast_Score=87, Evalue=5e-18,
Organism=Homo sapiens, GI4503423, Length=141, Percent_Identity=36.8794326241135, Blast_Score=87, Evalue=6e-18,
Organism=Homo sapiens, GI70906441, Length=140, Percent_Identity=37.1428571428571, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=75.4966887417219, Blast_Score=248, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI71988561, Length=147, Percent_Identity=38.7755102040816, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319729, Length=132, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24583610, Length=149, Percent_Identity=34.2281879194631, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921126, Length=149, Percent_Identity=34.2281879194631, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_HAEI8 (Q4QLV3)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248654.1
- ProteinModelPortal:   Q4QLV3
- SMR:   Q4QLV3
- STRING:   Q4QLV3
- GeneID:   3430419
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI1127
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   Q4QLV3
- ProtClustDB:   PRK00601
- BioCyc:   HINF281310:NTHI1127-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16445; Mature: 16445

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: BINDING 83-83 BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIDVKILDSRIGNEFPLPTYATEGSAGLDLRALIDESFEIQPGETKLIPTGLSIYIAD
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCEEEEECCCEEEEEC
PNLAAVILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSMWNRGNEPFKIEVGDRIAQLV
CCEEEEEEECCCCCCCCCEEEEHEEECCCCCCCCCEEEEEECCCCCCEEEEHHHHEEHHH
FVPVVQAEFNIVEDFQQTERGEGGFGHSGKQ
HEEHHHCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIDVKILDSRIGNEFPLPTYATEGSAGLDLRALIDESFEIQPGETKLIPTGLSIYIAD
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCEEEEECCCEEEEEC
PNLAAVILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSMWNRGNEPFKIEVGDRIAQLV
CCEEEEEEECCCCCCCCCEEEEHEEECCCCCCCCCEEEEEECCCCCCEEEEHHHHEEHHH
FVPVVQAEFNIVEDFQQTERGEGGFGHSGKQ
HEEHHHCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA