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The map label for this gene is hisH

Identifier: 148827670

GI number: 148827670

Start: 1021607

End: 1022206

Strand: Direct

Name: hisH

Synonym: CGSHiGG_05605

Alternate gene names: 148827670

Gene position: 1021607-1022206 (Clockwise)

Preceding gene: 148827669

Following gene: 148827671

Centisome position: 54.13

GC content: 38.17

Gene sequence:

>600_bases
ATGACAAACATCACGATTATAGACACAGGTTGTGCCAACCTTTCTTCTGTAAAATTCGCCTTTGACCGATTAGGTTACAA
TACCGAGATCACTTTTGATCTCAATAAAATCAAATCTGCCGATAAGCTGATTTTACCTGGCGTAGGCACTGCAAATGCAG
CAATGTATAATTTACAAGAACGTCAATTGATCGAAACCATTCAAAATCTAACACAACCAGTGCTAGGAATTTGTTTGGGT
ATGCAGTTAATGACCGAATTTTCCGAAGAGGGTAATGTGCCGACCTTAAACCTAATAAGCGGAAAAACTAATCGAATTCC
CGATACGGGTTTACCGTTACCTCAAATGGGATGGAATCGCGTGCAATTTGTAAAAAACTGTCCGTTATTTGATGGTATTG
TGCAAAATAGTCACTTCTATTTTGTGCATAGTTATGCAGTTAGCCCGAATGAACATTCGGTGGCAATAAGCAATTATGGT
GTGAATTTTTCTGCTGCGATAGCGAAAGAAAATTTTTACGGTGTACAATTTCATCCCGAACGTTCGGGTAAAAATGGTGC
GTTGTTGCTAAAAAATTTTGTGGAAAAAGTACCGTTTTAA

Upstream 100 bases:

>100_bases
GAAATGAAATGCCAAGTTCAAAAGGAGTTTTATAGTTGAAAGAGCGGTTAATTTTTGTCGTATTTTTGCAAAATTCGACC
GATTGTAAAAGGATAAAAAA

Downstream 100 bases:

>100_bases
TCACTAGATCATATAGTCATACAGGATCAAACACAATGAAACAATCTATTATTATCCCTGCTCTTGATCTTATCAATGGT
CAAGTTGTGCGGTTACACCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MTNITIIDTGCANLSSVKFAFDRLGYNTEITFDLNKIKSADKLILPGVGTANAAMYNLQERQLIETIQNLTQPVLGICLG
MQLMTEFSEEGNVPTLNLISGKTNRIPDTGLPLPQMGWNRVQFVKNCPLFDGIVQNSHFYFVHSYAVSPNEHSVAISNYG
VNFSAAIAKENFYGVQFHPERSGKNGALLLKNFVEKVPF

Sequences:

>Translated_199_residues
MTNITIIDTGCANLSSVKFAFDRLGYNTEITFDLNKIKSADKLILPGVGTANAAMYNLQERQLIETIQNLTQPVLGICLG
MQLMTEFSEEGNVPTLNLISGKTNRIPDTGLPLPQMGWNRVQFVKNCPLFDGIVQNSHFYFVHSYAVSPNEHSVAISNYG
VNFSAAIAKENFYGVQFHPERSGKNGALLLKNFVEKVPF
>Mature_198_residues
TNITIIDTGCANLSSVKFAFDRLGYNTEITFDLNKIKSADKLILPGVGTANAAMYNLQERQLIETIQNLTQPVLGICLGM
QLMTEFSEEGNVPTLNLISGKTNRIPDTGLPLPQMGWNRVQFVKNCPLFDGIVQNSHFYFVHSYAVSPNEHSVAISNYGV
NFSAAIAKENFYGVQFHPERSGKNGALLLKNFVEKVPF

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=194, Percent_Identity=56.1855670103093, Blast_Score=214, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=34.5794392523364, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_HAEI8 (Q4QN71)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248186.1
- ProteinModelPortal:   Q4QN71
- SMR:   Q4QN71
- STRING:   Q4QN71
- GeneID:   3429913
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0603
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   YSEEDDI
- PhylomeDB:   Q4QN71
- ProtClustDB:   PRK13170
- BioCyc:   HINF281310:NTHI0603-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR006220
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- PRINTS:   PR00097
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22037; Mature: 21905

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 78-78 ACT_SITE 178-178 ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNITIIDTGCANLSSVKFAFDRLGYNTEITFDLNKIKSADKLILPGVGTANAAMYNLQE
CCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEHHHCCCCCEEEECCCCCCCHHHHHHHH
RQLIETIQNLTQPVLGICLGMQLMTEFSEEGNVPTLNLISGKTNRIPDTGLPLPQMGWNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCH
VQFVKNCPLFDGIVQNSHFYFVHSYAVSPNEHSVAISNYGVNFSAAIAKENFYGVQFHPE
HHHHHCCCHHHHHHCCCEEEEEEEEEECCCCCEEEEEECCCCEEEEEECCCEEEEEEECC
RSGKNGALLLKNFVEKVPF
CCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TNITIIDTGCANLSSVKFAFDRLGYNTEITFDLNKIKSADKLILPGVGTANAAMYNLQE
CEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEHHHCCCCCEEEECCCCCCCHHHHHHHH
RQLIETIQNLTQPVLGICLGMQLMTEFSEEGNVPTLNLISGKTNRIPDTGLPLPQMGWNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCH
VQFVKNCPLFDGIVQNSHFYFVHSYAVSPNEHSVAISNYGVNFSAAIAKENFYGVQFHPE
HHHHHCCCHHHHHHCCCEEEEEEEEEECCCCCEEEEEECCCCEEEEEECCCEEEEEEECC
RSGKNGALLLKNFVEKVPF
CCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA