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The map label for this gene is xylF [H]

Identifier: 148826275

GI number: 148826275

Start: 1289124

End: 1290122

Strand: Direct

Name: xylF [H]

Synonym: CGSHiEE_06500

Alternate gene names: 148826275

Gene position: 1289124-1290122 (Clockwise)

Preceding gene: 148826271

Following gene: 148826276

Centisome position: 71.1

GC content: 33.43

Gene sequence:

>999_bases
ATGAAAATCAAATCAGCTTTACTTACCCTTGTTGGTGCATTAACTGTATTTAGTAGTTCTGCCCATTCCAAAGATCTTAA
AATTGGTTTATCCATTGATGATTTACGTTTAGAAAGATGGCAAAAAGATCGAGATATTTTCGTAAACAAAGCAGAATCAA
TGGGGGCAAAAGTGTTTGTCCAATCTGCGAATGGTGATGATTCAGCACAAATTTCTCAAATCGAAAATATGATCAATAAA
AATATTGATGTATTAGTAATTATTCCTCATAACGGTGAAGTATTAAGTAACGTTATTTCTGAAGCTAAAAAGGAAGGAAT
AAAAGTATTAGCGTATGACCGTTTAATCAATAATGCAGATTTAGATTTTTATGTTTCCTTTGATAATGAAAAAGTGGGTG
AACTACAAGCTAAAAGTATTGTAGCTGTAAAACCAGAAGGTAATTATTTTTTAATGGGGGGATCTCCTGTAGATAATAAT
GCAAAATTATTTAGAAAAGGGCAAATGAAAGTATTAGATCCTTTAATTGCCAGTGGGAAAATTAAGGTTGTAGGAGATCA
ATGGGTTGATTCTTGGTTAGCTGAAAAAGCGTTACAAATTATGGAAAACGCATTAACAGCTAATAAAAATAATGTGGATG
CTGTTGTTGCATCTAACGATGCTACTGCTGGAGGTGCGATTCAAGCCCTTAGCGCACAAGGCTTATCTGGGAAAGTCGCA
ATTTCTGGGCAAGATGCAGATTTAGCTGCAATTAAACGTATTGTCAATGGCTCGCAAACTATGACTGTTTATAAACCAAT
TACGAAACTTGCAGATAAAGCGGCAGAAATTGCGGTTGAATTAAGTAAGAATGAAAAAGTAGAAGCTAATGCAGAACTGA
ATAATGGCTTAAAAAATGTTCCTGCGTATTTACTTGATCCTATCGCTGTTGATAAACGTAATATTAATGAAACTGTAATT
AAAGATGGTTTTCATACTAAGGAAAGTATTTATCACTAA

Upstream 100 bases:

>100_bases
ATAATGGAGAAAGATTTTATAATTGAAAGCCTTTTTTAAAGTGTGAAAACTAACAACTGGAGCAATAACTAGTCCTTCAC
TTAACTATTGAGGTATCAAT

Downstream 100 bases:

>100_bases
CTTTAAGAGGGAAGAGAAAACTCTTCCCTTACCTCTTAGGGAGTTACGATATGGCATTGTTGGAAATGAAACATATCACA
AAAAAATTTGGTGATGTGAC

Product: D-xylose transporter subunit XylF

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKIKSALLTLVGALTVFSSSAHSKDLKIGLSIDDLRLERWQKDRDIFVNKAESMGAKVFVQSANGDDSAQISQIENMINK
NIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNADLDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNN
AKLFRKGQMKVLDPLIASGKIKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVA
ISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELSKNEKVEANAELNNGLKNVPAYLLDPIAVDKRNINETVI
KDGFHTKESIYH

Sequences:

>Translated_332_residues
MKIKSALLTLVGALTVFSSSAHSKDLKIGLSIDDLRLERWQKDRDIFVNKAESMGAKVFVQSANGDDSAQISQIENMINK
NIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNADLDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNN
AKLFRKGQMKVLDPLIASGKIKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVA
ISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELSKNEKVEANAELNNGLKNVPAYLLDPIAVDKRNINETVI
KDGFHTKESIYH
>Mature_332_residues
MKIKSALLTLVGALTVFSSSAHSKDLKIGLSIDDLRLERWQKDRDIFVNKAESMGAKVFVQSANGDDSAQISQIENMINK
NIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNADLDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNN
AKLFRKGQMKVLDPLIASGKIKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVA
ISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELSKNEKVEANAELNNGLKNVPAYLLDPIAVDKRNINETVI
KDGFHTKESIYH

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=329, Percent_Identity=75.6838905775076, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1790192, Length=294, Percent_Identity=24.4897959183673, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790526, Length=290, Percent_Identity=26.2068965517241, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35917; Mature: 35917

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKSALLTLVGALTVFSSSAHSKDLKIGLSIDDLRLERWQKDRDIFVNKAESMGAKVFV
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCCCHHEEECHHCCCEEEEE
QSANGDDSAQISQIENMINKNIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNAD
EECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCC
LDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNNAKLFRKGQMKVLDPLIASGK
EEEEEEECCCCCCCEEECEEEEECCCCCEEEECCCCCCCCHHHHCCCCHHHHHHHHCCCE
IKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVA
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHEECCCCCCCEEE
ISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELSKNEKVEANAELNNGLKNV
ECCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHEEEEECCCCCEEECCHHHCCHHHC
PAYLLDPIAVDKRNINETVIKDGFHTKESIYH
CHHHHCCHHCCCCCCCHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MKIKSALLTLVGALTVFSSSAHSKDLKIGLSIDDLRLERWQKDRDIFVNKAESMGAKVFV
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCCCHHEEECHHCCCEEEEE
QSANGDDSAQISQIENMINKNIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNAD
EECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCC
LDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNNAKLFRKGQMKVLDPLIASGK
EEEEEEECCCCCCCEEECEEEEECCCCCEEEECCCCCCCCHHHHCCCCHHHHHHHHCCCE
IKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVA
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHEECCCCCCCEEE
ISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELSKNEKVEANAELNNGLKNV
ECCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHEEEEECCCCCEEECCHHHCCHHHC
PAYLLDPIAVDKRNINETVIKDGFHTKESIYH
CHHHHCCHHCCCCCCCHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]