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The map label for this gene is rfaD [H]

Identifier: 148826271

GI number: 148826271

Start: 1285109

End: 1285981

Strand: Direct

Name: rfaD [H]

Synonym: CGSHiEE_06480

Alternate gene names: 148826271

Gene position: 1285109-1285981 (Clockwise)

Preceding gene: 148826270

Following gene: 148826275

Centisome position: 70.88

GC content: 40.09

Gene sequence:

>873_bases
TTGAATGATTTAGGGCGCAAAGATATTTTAGTGGTGGATAACTTAAAAGATGGCACGAAATTTGCAAATTTAGTTGATTT
AGACATCGCAGATTATTGCGATAAAGAAGATTTCATTGCTTCTATTATTGCGGGCGATGAATTTGGCGATATTGATGCGG
TATTCCACGAGGGGGCATGTTCTGCGACTACGGAATGGGATGGTAAATACATTATGCACAACAACTACGAATATTCTAAA
GAGTTGTTGCATTATTGCCTTGACCGTGAAATTCCTTTTTTCTATGCCTCAAGCGCAGCAACTTATGGAGACACCAAAGT
ATTCCGTGAAGAACGTGAATTTGAAGGCCCATTAAATGTTTATGGCTATTCTAAATTCTTGTTCGATCAATATGTACGCA
ACATTTTGCCAGAAGCAAAATCGCCAGTATGTGGTTTCCGTTATTTCAATGTTTACGGGCCGCGTGAAAATCATAAAGGT
TCTATGGCGAGTGTGGCATTCCACTTGAATAACCAAATCTTAAAAGGCGAAAATCCAAAATTATTTGCAGGCAGCGAAGG
ATTCCGCCGTGATTTTGTTTATGTGGGCGATGTGGCTGCAGTGAATATTTGGTGTTGGCAAAATGGTATTTCGGGTATTT
ACAACCTTGGTACAGGAAATGCAGAAAGTTTCCGTGCGGTGGCTGATGCGGTAGTGAAATTCCACGGTAAAGGCAAGATT
GAAACCATTCCATTCCCAGAGCATTTGAAATCTCGTTATCAAGAATATACTCAAGCAGATTTAACTAAACTTCGCTCAAC
AGGCTACGATAAACCATTCAAAACAGTCGCGGAAGGCGTGGCTGAATATATGGCGTGGTTGAATAGAAAATAA

Upstream 100 bases:

>100_bases
AAAATCTATTACAATACGCAGACTTATTTTAAAGAGGACAGAATTATGATTATTGTAACAGGCGGCGCTTGGTTTTATTG
GCAGTAATATTGTCAAAGCA

Downstream 100 bases:

>100_bases
ATTATTAATGTAGTAAATCCCCTATAGCTTATCGGTATAGGGGATTTTATTTAATATAATTGTGATAAATGTGTTAAATA
ACGCTCATAAGCACTTTGAT

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDEFGDIDAVFHEGACSATTEWDGKYIMHNNYEYSK
ELLHYCLDREIPFFYASSAATYGDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSPVCGFRYFNVYGPRENHKG
SMASVAFHLNNQILKGENPKLFAGSEGFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAESFRAVADAVVKFHGKGKI
ETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVAEYMAWLNRK

Sequences:

>Translated_290_residues
MNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDEFGDIDAVFHEGACSATTEWDGKYIMHNNYEYSK
ELLHYCLDREIPFFYASSAATYGDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSPVCGFRYFNVYGPRENHKG
SMASVAFHLNNQILKGENPKLFAGSEGFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAESFRAVADAVVKFHGKGKI
ETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVAEYMAWLNRK
>Mature_290_residues
MNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDEFGDIDAVFHEGACSATTEWDGKYIMHNNYEYSK
ELLHYCLDREIPFFYASSAATYGDTKVFREEREFEGPLNVYGYSKFLFDQYVRNILPEAKSPVCGFRYFNVYGPRENHKG
SMASVAFHLNNQILKGENPKLFAGSEGFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAESFRAVADAVVKFHGKGKI
ETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVAEYMAWLNRK

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790049, Length=290, Percent_Identity=76.2068965517241, Blast_Score=469, Evalue=1e-134,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.20 [H]

Molecular weight: Translated: 32958; Mature: 32958

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDEFGDIDAVFHEGAC
CCCCCCCCEEEEECCCCCCHHHHEEECCHHHHCCHHHHHHHHHCCCCCCCHHHHHHCCCC
SATTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATYGDTKVFREEREFEGPLNV
CCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCH
YGYSKFLFDQYVRNILPEAKSPVCGFRYFNVYGPRENHKGSMASVAFHLNNQILKGENPK
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCEEECCCCCC
LFAGSEGFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAESFRAVADAVVKFHGKGKI
EEECCCCCCCCEEEECCEEEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCE
ETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVAEYMAWLNRK
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDLGRKDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDEFGDIDAVFHEGAC
CCCCCCCCEEEEECCCCCCHHHHEEECCHHHHCCHHHHHHHHHCCCCCCCHHHHHHCCCC
SATTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATYGDTKVFREEREFEGPLNV
CCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCH
YGYSKFLFDQYVRNILPEAKSPVCGFRYFNVYGPRENHKGSMASVAFHLNNQILKGENPK
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCEEECCCCCC
LFAGSEGFRRDFVYVGDVAAVNIWCWQNGISGIYNLGTGNAESFRAVADAVVKFHGKGKI
EEECCCCCCCCEEEECCEEEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCE
ETIPFPEHLKSRYQEYTQADLTKLRSTGYDKPFKTVAEGVAEYMAWLNRK
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA