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The map label for this gene is spoT [H]

Identifier: 148825721

GI number: 148825721

Start: 668548

End: 670662

Strand: Direct

Name: spoT [H]

Synonym: CGSHiEE_03330

Alternate gene names: 148825721

Gene position: 668548-670662 (Clockwise)

Preceding gene: 148825720

Following gene: 148825722

Centisome position: 36.87

GC content: 40.28

Gene sequence:

>2115_bases
TTGGAACTGTTTGCGGATTTAGATCGAATTATTCAAGGGTATTTGCCCGCAGATAAAATTGAACTCATCAAGCGTGCGTT
TGTGATTGCAAGAGATGCGCACGAAGGACAATTTCGCTCCAGCGGCGAACCTTACATTACCCATCCTGTGGCGGTAGCCT
CCATTATTGCTCAACTTCACTTAGATCACGAAGCGGTAATGGCTGCGCTTTTGCACGATGTTATTGAAGATACACCTTAT
ACGGAAGAACAACTGAAAGAAGAGTTCGGTGCCAGTGTAGCTGAAATTGTGGACGGTGTATCAAAACTCGATAAGTTGAA
ATTTCGTACTCGCCAAGAAGCACAAGTCGAGAACTTTCGCAAAATGATTTTGGCGATGACGCGTGATATTCGTGTTGTGT
TAATCAAGCTTGCTGACCGCACGCATAATATGCGCACGCTTGGTTCGTTACGACCAGATAAAGGCCGCCGTATTGCGAAA
GAAACGCTTGAAATCTATTGTCCGCTTGCTCATCGTTTAGGTATTGAGCATATCAAAAATGAATTAGAAGACTTATCTTT
TCAGGCAATGCATCCACACCGTTATGAGGTGTTAAAAAAACTCATTGATGTGGCGCGGAGTAATCGACAAGATTTAATCG
AACGTATTTCACAAGAGATTAAAGTGCGGTTAGAAAACGCAGGCATTTTTGCCCGAGTGTGGGGACGTGAAAAACACCTT
TACAAGATCTATCAAAAAATGCGCATAAAGGATCAAGAATTTCATTCTATTATGGATATTTATGCGTTCCGTGTGATTGT
AAAAAATGTTGATGATTGTTATCGCGTTTTGGGACAAATGCATAATCTTTATAAGCCACGCCCTGGCCGAGTAAAGGATT
ATATCGCGGTACCTAAAGCAAATGGCTATCAATCTTTGCAAACTTCAATGATTGGGCCGAAAGGAGTTCCCGTAGAAGTT
CATATCCATACTGAGGATATGGAACAAGTCGCTGAAATGGGCATTACTGCGCATTGGGTTTATAAAGAAAATGGTAAAAA
CGATAGTACAACCGCACAAATCCGCGTACAACGCTGGTTGCAAAGTTTGGTCGAAATTCAACAAAGTGTAGGTAATTCGT
TTGAGTTTATTGAGAATGTAAAATCAGAGTTTTTCCCGAAAGAAATTTATGTTTTTACGCCAAAAGGTCGAATTGTTGAA
TTACCAATGGGCGCAACAGCAGTAGATTTCGCTTATGCTGTGCATTCAGATGTGGGGAATATTTGTGTTGGCGTAACCGT
TGAGCATAAACCTTATCCGCTTTCAAAAGCCCTTGAATCAGGGCAAACAGTCAATATCATTACTGATCCAAATGCGCATC
CTGAAGTCGCATGGTTAAATTTTGTGGTAACGGCTCGTGCGAAAACACGGATTCGCCATTATCTCAAACAGCGTTGTGAA
GAGGATGCGGTGAAATTAGGCGAAGTTGAGCTTAATGTAGCATTACAGCCGCATAATTTAGGTGATTTTTCTATTCAACA
AATTAGCACTGTATTAGATGCTTTAGCCCTTTCTTCTTTAGATGAATTATTACGAGAAATTGGTTTAGGTAATCAGTCAG
CCTCCATGATTGCGCATCAATTTGTGGGCGTGCCGTTAGAATCTGCTAATACTAAGAATCTCGAATTTGAATCAAAAATA
CTCACTATTGCACCAATGCAAGTGGGTAAAACACAATTTGCGCAATGTTGCCATCCAATCCTAGGTGATCCTATTGTGGG
ATGTAGTACAGAAAAAAATACAGTTGTGGTACACCATCAACATTGTGCAAGTTTGAAAAATGCTTGTCGTCAATCGTTGG
CTAAATGGGATAACGTTCAAAGTGCGGTGAATTTTGAGGCTGAATTACAAATAGAAATATTGAATGAGCAGAATGCCTTG
CTTAGTTTAATGACCGCAATTTCAGCGAGCGAAAGCAGTCTCCAAAATATTTGGACGGAAGAATTAGAGAACAATTTATT
GCTTGTGATTTTGCAGGTTTGTGTAAAAGATATCAAACACCTTGCCAATATCGTTCATCGTATTAAAGGCATCACTGGCG
TCGTTAATGTCAAACGCAATATTAATGAGCTATGA

Upstream 100 bases:

>100_bases
AGCAGCTGTTGCATTAATTACAGAATAATATTTTAAAAGTGCGGTAAAAAATCACGATATTTTAACTGCACTTTCTCTTT
CAACGATAAGTAGGTCTCCT

Downstream 100 bases:

>100_bases
GCCTTGAACTTCTCGATTCCGTGCCTTTGACCAGCCTTTCTGGTGTGGGGGCTGCCATTTCTAATAAACTGGCTAAAATC
GGCATTCATAACCTGCAGGA

Product: guanosine-3',5'-bis 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLHLDHEAVMAALLHDVIEDTPY
TEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKGRRIAK
ETLEIYCPLAHRLGIEHIKNELEDLSFQAMHPHRYEVLKKLIDVARSNRQDLIERISQEIKVRLENAGIFARVWGREKHL
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKANGYQSLQTSMIGPKGVPVEV
HIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWLQSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVE
LPMGATAVDFAYAVHSDVGNICVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
EDAVKLGEVELNVALQPHNLGDFSIQQISTVLDALALSSLDELLREIGLGNQSASMIAHQFVGVPLESANTKNLEFESKI
LTIAPMQVGKTQFAQCCHPILGDPIVGCSTEKNTVVVHHQHCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNAL
LSLMTAISASESSLQNIWTEELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL

Sequences:

>Translated_704_residues
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLHLDHEAVMAALLHDVIEDTPY
TEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKGRRIAK
ETLEIYCPLAHRLGIEHIKNELEDLSFQAMHPHRYEVLKKLIDVARSNRQDLIERISQEIKVRLENAGIFARVWGREKHL
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKANGYQSLQTSMIGPKGVPVEV
HIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWLQSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVE
LPMGATAVDFAYAVHSDVGNICVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
EDAVKLGEVELNVALQPHNLGDFSIQQISTVLDALALSSLDELLREIGLGNQSASMIAHQFVGVPLESANTKNLEFESKI
LTIAPMQVGKTQFAQCCHPILGDPIVGCSTEKNTVVVHHQHCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNAL
LSLMTAISASESSLQNIWTEELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL
>Mature_704_residues
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLHLDHEAVMAALLHDVIEDTPY
TEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKGRRIAK
ETLEIYCPLAHRLGIEHIKNELEDLSFQAMHPHRYEVLKKLIDVARSNRQDLIERISQEIKVRLENAGIFARVWGREKHL
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKANGYQSLQTSMIGPKGVPVEV
HIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWLQSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVE
LPMGATAVDFAYAVHSDVGNICVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
EDAVKLGEVELNVALQPHNLGDFSIQQISTVLDALALSSLDELLREIGLGNQSASMIAHQFVGVPLESANTKNLEFESKI
LTIAPMQVGKTQFAQCCHPILGDPIVGCSTEKNTVVVHHQHCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNAL
LSLMTAISASESSLQNIWTEELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=711, Percent_Identity=56.9620253164557, Blast_Score=831, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=686, Percent_Identity=31.3411078717201, Blast_Score=347, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79683; Mature: 79683

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLH
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHH
LDHEAVMAALLHDVIEDTPYTEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKGRRIAKETLEIYCPLAHRLGIEHIKN
HHHHHHHCCHHHHHEEHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCHHHHCCHHHHHH
ELEDLSFQAMHPHRYEVLKKLIDVARSNRQDLIERISQEIKVRLENAGIFARVWGREKHL
HHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEHHCCCEEEEECCCHHHH
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLQTSMIGPKGVPVEVHIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWL
CCHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHH
QSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVELPMGATAVDFAYAVHSDVGN
HHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHCCCC
ICVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
EEEEEEECCCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDAVKLGEVELNVALQPHNLGDFSIQQISTVLDALALSSLDELLREIGLGNQSASMIAHQ
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FVGVPLESANTKNLEFESKILTIAPMQVGKTQFAQCCHPILGDPIVGCSTEKNTVVVHHQ
HHCCCCCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHH
HCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNALLSLMTAISASESSLQNIWTE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHH
ELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHCCC
>Mature Secondary Structure
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLH
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHH
LDHEAVMAALLHDVIEDTPYTEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKGRRIAKETLEIYCPLAHRLGIEHIKN
HHHHHHHCCHHHHHEEHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCHHHHCCHHHHHH
ELEDLSFQAMHPHRYEVLKKLIDVARSNRQDLIERISQEIKVRLENAGIFARVWGREKHL
HHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEHHCCCEEEEECCCHHHH
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLQTSMIGPKGVPVEVHIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWL
CCHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHH
QSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVELPMGATAVDFAYAVHSDVGN
HHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHCCCC
ICVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
EEEEEEECCCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDAVKLGEVELNVALQPHNLGDFSIQQISTVLDALALSSLDELLREIGLGNQSASMIAHQ
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FVGVPLESANTKNLEFESKILTIAPMQVGKTQFAQCCHPILGDPIVGCSTEKNTVVVHHQ
HHCCCCCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHH
HCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNALLSLMTAISASESSLQNIWTE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHH
ELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]