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The map label for this gene is rpoZ [H]

Identifier: 148825722

GI number: 148825722

Start: 670659

End: 672740

Strand: Direct

Name: rpoZ [H]

Synonym: CGSHiEE_03335

Alternate gene names: 148825722

Gene position: 670659-672740 (Clockwise)

Preceding gene: 148825721

Following gene: 148825723

Centisome position: 36.99

GC content: 41.4

Gene sequence:

>2082_bases
ATGAGCCTTGAACTTCTCGATTCCGTGCCTTTGACCAGCCTTTCTGGTGTGGGGGCTGCCATTTCTAATAAACTGGCTAA
AATCGGCATTCATAACCTGCAGGATTTACTCTTTCATTTACCCATTCGCTATGAAGATCGTACGCGAATTACGCCAATCG
CTAATCTTCGCCCTGAACAATATTTTACTATAGAGGGAATTGTACAAACCTGTGAAGTTGCTTTTGGCCGTCGCCCGATT
TTATCGGTAAGTTTGTCAGACGGCACATCGAAAATTATGTTACGTTTTTTCAATTTTAATGCGGGAATGCGTAATAGTTT
TCAAGTTGGCGTGCGAGTAAAGGCTTTTGGCGAAGTGAAACGTGGTCGCTATATGCCCGAAATTCATCATCCAGAATATC
AAATTGTGCGAGATAATGCGCCCATAGTATTGGAAGAAACCCTAACGCCGATTTATTCCACAACGGAAGGGCTAAAACAA
AATTCATTACGAAAATTAACGGATCAGGCTTTGGCATTACTCGACAAAGTTCAGATTGCAGAAATTTTGCCTAATGAATT
TAATCCACATCAATATAGCTTAAAAGAAGCATTGCGATTATTGCATCGTCCGCCGCCAGATATTTCATTAGAAATGTTAG
AGCAAGGTAAGCATCCTGCACAACAACGATTAATTTTTGAAGAGCTTCTGGCACATAATTTAGCTATGCAAAAAGTGCGG
TTAGGAACGCAACAATTTTCTGCCTTACCGCTACATTATCAAACAGATTTGAAACAGCGATTTTTAGCCACCTTGCCATT
TCAACCCACTAATGCGCAAAAGCGAGTCGTGTCGGATATTGAACAAGATCTTATCAAAGATTATCCGATGATGCGCTTAG
TGCAAGGGGATGTAGGCTCGGGAAAAACATTGGTTGCCGCATTGGCTGCTTTAACTGCAATTGATAATGGCAAACAAGTC
GCCTTAATGGCACCAACAGAAATTCTTGCAGAACAGCATGCGAATAATTTTAGACAGTGGTTTGAGCCTTTCGGTATTGA
AGTTGGCTGGTTGGCAGGTAAAGTAAAAGGAAAATCAAGACAAGCAGAGCTTGAAAAAATTAAAACTGGCACGGTGCAAA
TGGTGGTGGGGACGCATGCTTTATTCCAAGAAGAAGTCGAGTTTTCCGACTTAGCATTAGTGATTATTGATGAACAACAT
CGTTTTGGGGTGCATCAACGATTAATGTTGCGTGAAAAAGGCGAAAAAGCGGGATTTTATCCCCATCAGTTAATTATGAC
GGCCACGCCGATTCCGAGAACGTTGGCAATGACCGTATATGCAGATTTGGATACATCAATTATTGATGAATTGCCTCCAG
GTCGTACGCCGATAACAACAGTAGTGGTTTCTGAAGAACGTCGCGCTGAAATTGTGATGCGTGTAAAAAACGCTTGTGTT
AATGAAAAACGTCAAGCCTATTGGGTGTGTACGTTAATTGATGAATCAGAGGTGTTAGAAGCTCAAGCAGCTGAAGCGAT
TTGGGAAGATTTAACGAAAGCGTTACCGATGTTAAATATTGGTTTAGTGCACGGGCGCATGAAACCCCAAGAAAAACAAG
ATGTCATGATGCGTTTTAAAAATGCAGAATTGGATTTATTGGTGGCGACGACAGTCATCGAAGTGGGTGTTGATGTTCCG
AATGCTAGTTTGATGATTATCGAAAATGCAGAACGTTTAGGTTTATCGCAGCTTCATCAGTTACGAGGGCGCGTCGGTCG
AGGCAGCACGGCTTCTTTTTGTGTACTAATGTATAAACCTCCGCTCGGCAAAGTTTCGCAAAAACGTTTGCAAGTGCTAC
GAGATAGCCAAGATGGGTTTGTTATTTCGGAAAAAGATTTAGAAATTCGTGGGCCAGGTGAAGTACTTGGAACGAAACAA
ACGGGAATTGCTGAATTGCGAGTAGCAAATTTAATGCGAGATAGAAAAATGATACCAACGGTGCAACATTATGCGAAATC
ACTTATCCAAAAATACCCAGATCTAGCAGAAAGTCTGATTCGTCGTTGGTTAAATAATAAAGAGATTTACTCGAACGCTT
AA

Upstream 100 bases:

>100_bases
TGCAGGTTTGTGTAAAAGATATCAAACACCTTGCCAATATCGTTCATCGTATTAAAGGCATCACTGGCGTCGTTAATGTC
AAACGCAATATTAATGAGCT

Downstream 100 bases:

>100_bases
AAGTGCGGTCAATTTTCAAAGAGTTTTAAATGAATAAAAAAGAAAAACGCCCAACTGTACTTTTTTTTGACTCTGGAGTG
GGTGGGTTTAGCGTATACCG

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 693; Mature: 692

Protein sequence:

>693_residues
MSLELLDSVPLTSLSGVGAAISNKLAKIGIHNLQDLLFHLPIRYEDRTRITPIANLRPEQYFTIEGIVQTCEVAFGRRPI
LSVSLSDGTSKIMLRFFNFNAGMRNSFQVGVRVKAFGEVKRGRYMPEIHHPEYQIVRDNAPIVLEETLTPIYSTTEGLKQ
NSLRKLTDQALALLDKVQIAEILPNEFNPHQYSLKEALRLLHRPPPDISLEMLEQGKHPAQQRLIFEELLAHNLAMQKVR
LGTQQFSALPLHYQTDLKQRFLATLPFQPTNAQKRVVSDIEQDLIKDYPMMRLVQGDVGSGKTLVAALAALTAIDNGKQV
ALMAPTEILAEQHANNFRQWFEPFGIEVGWLAGKVKGKSRQAELEKIKTGTVQMVVGTHALFQEEVEFSDLALVIIDEQH
RFGVHQRLMLREKGEKAGFYPHQLIMTATPIPRTLAMTVYADLDTSIIDELPPGRTPITTVVVSEERRAEIVMRVKNACV
NEKRQAYWVCTLIDESEVLEAQAAEAIWEDLTKALPMLNIGLVHGRMKPQEKQDVMMRFKNAELDLLVATTVIEVGVDVP
NASLMIIENAERLGLSQLHQLRGRVGRGSTASFCVLMYKPPLGKVSQKRLQVLRDSQDGFVISEKDLEIRGPGEVLGTKQ
TGIAELRVANLMRDRKMIPTVQHYAKSLIQKYPDLAESLIRRWLNNKEIYSNA

Sequences:

>Translated_693_residues
MSLELLDSVPLTSLSGVGAAISNKLAKIGIHNLQDLLFHLPIRYEDRTRITPIANLRPEQYFTIEGIVQTCEVAFGRRPI
LSVSLSDGTSKIMLRFFNFNAGMRNSFQVGVRVKAFGEVKRGRYMPEIHHPEYQIVRDNAPIVLEETLTPIYSTTEGLKQ
NSLRKLTDQALALLDKVQIAEILPNEFNPHQYSLKEALRLLHRPPPDISLEMLEQGKHPAQQRLIFEELLAHNLAMQKVR
LGTQQFSALPLHYQTDLKQRFLATLPFQPTNAQKRVVSDIEQDLIKDYPMMRLVQGDVGSGKTLVAALAALTAIDNGKQV
ALMAPTEILAEQHANNFRQWFEPFGIEVGWLAGKVKGKSRQAELEKIKTGTVQMVVGTHALFQEEVEFSDLALVIIDEQH
RFGVHQRLMLREKGEKAGFYPHQLIMTATPIPRTLAMTVYADLDTSIIDELPPGRTPITTVVVSEERRAEIVMRVKNACV
NEKRQAYWVCTLIDESEVLEAQAAEAIWEDLTKALPMLNIGLVHGRMKPQEKQDVMMRFKNAELDLLVATTVIEVGVDVP
NASLMIIENAERLGLSQLHQLRGRVGRGSTASFCVLMYKPPLGKVSQKRLQVLRDSQDGFVISEKDLEIRGPGEVLGTKQ
TGIAELRVANLMRDRKMIPTVQHYAKSLIQKYPDLAESLIRRWLNNKEIYSNA
>Mature_692_residues
SLELLDSVPLTSLSGVGAAISNKLAKIGIHNLQDLLFHLPIRYEDRTRITPIANLRPEQYFTIEGIVQTCEVAFGRRPIL
SVSLSDGTSKIMLRFFNFNAGMRNSFQVGVRVKAFGEVKRGRYMPEIHHPEYQIVRDNAPIVLEETLTPIYSTTEGLKQN
SLRKLTDQALALLDKVQIAEILPNEFNPHQYSLKEALRLLHRPPPDISLEMLEQGKHPAQQRLIFEELLAHNLAMQKVRL
GTQQFSALPLHYQTDLKQRFLATLPFQPTNAQKRVVSDIEQDLIKDYPMMRLVQGDVGSGKTLVAALAALTAIDNGKQVA
LMAPTEILAEQHANNFRQWFEPFGIEVGWLAGKVKGKSRQAELEKIKTGTVQMVVGTHALFQEEVEFSDLALVIIDEQHR
FGVHQRLMLREKGEKAGFYPHQLIMTATPIPRTLAMTVYADLDTSIIDELPPGRTPITTVVVSEERRAEIVMRVKNACVN
EKRQAYWVCTLIDESEVLEAQAAEAIWEDLTKALPMLNIGLVHGRMKPQEKQDVMMRFKNAELDLLVATTVIEVGVDVPN
ASLMIIENAERLGLSQLHQLRGRVGRGSTASFCVLMYKPPLGKVSQKRLQVLRDSQDGFVISEKDLEIRGPGEVLGTKQT
GIAELRVANLMRDRKMIPTVQHYAKSLIQKYPDLAESLIRRWLNNKEIYSNA

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=693, Percent_Identity=67.0995670995671, Blast_Score=945, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=392, Percent_Identity=38.5204081632653, Blast_Score=251, Evalue=9e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78114; Mature: 77983

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLELLDSVPLTSLSGVGAAISNKLAKIGIHNLQDLLFHLPIRYEDRTRITPIANLRPEQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
YFTIEGIVQTCEVAFGRRPILSVSLSDGTSKIMLRFFNFNAGMRNSFQVGVRVKAFGEVK
EEEHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEEEEEEHHHHH
RGRYMPEIHHPEYQIVRDNAPIVLEETLTPIYSTTEGLKQNSLRKLTDQALALLDKVQIA
CCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EILPNEFNPHQYSLKEALRLLHRPPPDISLEMLEQGKHPAQQRLIFEELLAHNLAMQKVR
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LGTQQFSALPLHYQTDLKQRFLATLPFQPTNAQKRVVSDIEQDLIKDYPMMRLVQGDVGS
HCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
GKTLVAALAALTAIDNGKQVALMAPTEILAEQHANNFRQWFEPFGIEVGWLAGKVKGKSR
CHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCH
QAELEKIKTGTVQMVVGTHALFQEEVEFSDLALVIIDEQHRFGVHQRLMLREKGEKAGFY
HHHHHHHCCCEEEEEEHHHHHHHHHCCCCCEEEEEECCCHHCCHHHHHHHHHCCCCCCCC
PHQLIMTATPIPRTLAMTVYADLDTSIIDELPPGRTPITTVVVSEERRAEIVMRVKNACV
CCEEEEECCCCCCEEEEEEEECCCHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
NEKRQAYWVCTLIDESEVLEAQAAEAIWEDLTKALPMLNIGLVHGRMKPQEKQDVMMRFK
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHEEHEEECCCCCCHHHHHHHHHC
NAELDLLVATTVIEVGVDVPNASLMIIENAERLGLSQLHQLRGRVGRGSTASFCVLMYKP
CCCEEEEHHHHHHHHCCCCCCCEEEEEECHHHCCHHHHHHHHHCCCCCCCCEEEEEEECC
PLGKVSQKRLQVLRDSQDGFVISEKDLEIRGPGEVLGTKQTGIAELRVANLMRDRKMIPT
CCCHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
VQHYAKSLIQKYPDLAESLIRRWLNNKEIYSNA
HHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SLELLDSVPLTSLSGVGAAISNKLAKIGIHNLQDLLFHLPIRYEDRTRITPIANLRPEQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
YFTIEGIVQTCEVAFGRRPILSVSLSDGTSKIMLRFFNFNAGMRNSFQVGVRVKAFGEVK
EEEHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEEEEEEHHHHH
RGRYMPEIHHPEYQIVRDNAPIVLEETLTPIYSTTEGLKQNSLRKLTDQALALLDKVQIA
CCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EILPNEFNPHQYSLKEALRLLHRPPPDISLEMLEQGKHPAQQRLIFEELLAHNLAMQKVR
HHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LGTQQFSALPLHYQTDLKQRFLATLPFQPTNAQKRVVSDIEQDLIKDYPMMRLVQGDVGS
HCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
GKTLVAALAALTAIDNGKQVALMAPTEILAEQHANNFRQWFEPFGIEVGWLAGKVKGKSR
CHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCH
QAELEKIKTGTVQMVVGTHALFQEEVEFSDLALVIIDEQHRFGVHQRLMLREKGEKAGFY
HHHHHHHCCCEEEEEEHHHHHHHHHCCCCCEEEEEECCCHHCCHHHHHHHHHCCCCCCCC
PHQLIMTATPIPRTLAMTVYADLDTSIIDELPPGRTPITTVVVSEERRAEIVMRVKNACV
CCEEEEECCCCCCEEEEEEEECCCHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
NEKRQAYWVCTLIDESEVLEAQAAEAIWEDLTKALPMLNIGLVHGRMKPQEKQDVMMRFK
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHEEHEEECCCCCCHHHHHHHHHC
NAELDLLVATTVIEVGVDVPNASLMIIENAERLGLSQLHQLRGRVGRGSTASFCVLMYKP
CCCEEEEHHHHHHHHCCCCCCCEEEEEECHHHCCHHHHHHHHHCCCCCCCCEEEEEEECC
PLGKVSQKRLQVLRDSQDGFVISEKDLEIRGPGEVLGTKQTGIAELRVANLMRDRKMIPT
CCCHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
VQHYAKSLIQKYPDLAESLIRRWLNNKEIYSNA
HHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]