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The map label for this gene is lipA

Identifier: 148825693

GI number: 148825693

Start: 638104

End: 639066

Strand: Direct

Name: lipA

Synonym: CGSHiEE_03180

Alternate gene names: 148825693

Gene position: 638104-639066 (Clockwise)

Preceding gene: 148825692

Following gene: 148825694

Centisome position: 35.2

GC content: 39.77

Gene sequence:

>963_bases
ATGTCTACGCCTTTCAAAATGGAACGCGGTGTAAAATATCGCGATGCGGCAAAAACCTCAATTATTCCCGTAAAAAATAT
CGATCCTAATCAAGATTTATTAAAAAAACCAGAATGGATGAAAATTAAACTTCCAGCAAGTTCAGCTAAAATTGAAAGCA
TTAAAAATGGAATGCGCCGTCACGGATTACATTCTGTTTGTGAGGAAGCATCTTGTCCAAATTTACACGAATGTTTTAAT
CATGGCACAGCCACTTTTATGATTTTAGGTGCAATTTGTACGCGCCGTTGCCCATTCTGTGATGTGGCTCACGGTAAACC
ATTACCGCCAGATCCTGAAGAGCCACAAAAATTGGCAGAAACGATTCAAGATATGAAACTTAAATATGTGGTAATCACAT
CAGTGGATCGTGATGACTTGCCTGATCGTGGTGCTGGTCATTTTTCTGAATGTGTAAAAGCTGTGCGTGAGCTTAATCCT
AACATTAAGATTGAAATTTTAGTGCCTGATTTTCGTGGTCGCGTTACACAGGCTTTAGAAAAATTAAAAGATAATCCGCC
AGATGTTTTTAACCATAATTTAGAAAACGTACCGCGCTTATATAAAGAAATTCGTCCAGGTGCGGATTACGGATGGTCAC
TTAAATTATTGCGTGAATTCAAAGAAATGTTCCCAAATATCCCAACCAAATCTGGTTTAATGGTTGGGTTAGGCGAAACC
AACGAGGAAATTTTGCAAGTGATGCAAGATTTACGTGATAACGGTGTGACTATGCTCACGTTGGGTCAATATCTTCAACC
TAGCCGTCATCATTTGCCTGTTGCCCGTTATGTGCCGCCAACTGAATTTGATGAATTCCGTGATAAAGCCAATGAAATGG
GCTTTGAACATGCGGCTTGTGGGCCATTTGTTCGTTCTTCTTATCACGCTGATTTACAAGCCAGTGGCGGATTAGTGAAA
TAA

Upstream 100 bases:

>100_bases
TTAAACACTTCGCCGATCTTTTAGGCTATAATATAACAACTTTATAACATTTTATAAAAACTAAATGCCCAATTTGGGCA
TTTTTATTTAGGAAAATACT

Downstream 100 bases:

>100_bases
TTTAAAATTCCGCTAAATTAAACCGCACTTTTTGCTTCTTACAAAAGTGCGGTTTATTTTTACAACTCAGAATAACCGCC
AATATTGCCTATAAATTATT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSTPFKMERGVKYRDAAKTSIIPVKNIDPNQDLLKKPEWMKIKLPASSAKIESIKNGMRRHGLHSVCEEASCPNLHECFN
HGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPQKLAETIQDMKLKYVVITSVDRDDLPDRGAGHFSECVKAVRELNP
NIKIEILVPDFRGRVTQALEKLKDNPPDVFNHNLENVPRLYKEIRPGADYGWSLKLLREFKEMFPNIPTKSGLMVGLGET
NEEILQVMQDLRDNGVTMLTLGQYLQPSRHHLPVARYVPPTEFDEFRDKANEMGFEHAACGPFVRSSYHADLQASGGLVK

Sequences:

>Translated_320_residues
MSTPFKMERGVKYRDAAKTSIIPVKNIDPNQDLLKKPEWMKIKLPASSAKIESIKNGMRRHGLHSVCEEASCPNLHECFN
HGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPQKLAETIQDMKLKYVVITSVDRDDLPDRGAGHFSECVKAVRELNP
NIKIEILVPDFRGRVTQALEKLKDNPPDVFNHNLENVPRLYKEIRPGADYGWSLKLLREFKEMFPNIPTKSGLMVGLGET
NEEILQVMQDLRDNGVTMLTLGQYLQPSRHHLPVARYVPPTEFDEFRDKANEMGFEHAACGPFVRSSYHADLQASGGLVK
>Mature_319_residues
STPFKMERGVKYRDAAKTSIIPVKNIDPNQDLLKKPEWMKIKLPASSAKIESIKNGMRRHGLHSVCEEASCPNLHECFNH
GTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPQKLAETIQDMKLKYVVITSVDRDDLPDRGAGHFSECVKAVRELNPN
IKIEILVPDFRGRVTQALEKLKDNPPDVFNHNLENVPRLYKEIRPGADYGWSLKLLREFKEMFPNIPTKSGLMVGLGETN
EEILQVMQDLRDNGVTMLTLGQYLQPSRHHLPVARYVPPTEFDEFRDKANEMGFEHAACGPFVRSSYHADLQASGGLVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=284, Percent_Identity=44.3661971830986, Blast_Score=250, Evalue=9e-67,
Organism=Homo sapiens, GI37577164, Length=248, Percent_Identity=44.3548387096774, Blast_Score=215, Evalue=4e-56,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=74.1433021806854, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=41.6083916083916, Blast_Score=225, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=41.5841584158416, Blast_Score=240, Evalue=2e-64,
Organism=Drosophila melanogaster, GI221513272, Length=284, Percent_Identity=45.4225352112676, Blast_Score=246, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_HAEIE (A5UBB2)

Other databases:

- EMBL:   CP000671
- RefSeq:   YP_001290446.1
- ProteinModelPortal:   A5UBB2
- STRING:   A5UBB2
- GeneID:   5225323
- GenomeReviews:   CP000671_GR
- KEGG:   hip:CGSHiEE_03180
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   HINF374930:CGSHIEE_03180-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36129; Mature: 35998

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPFKMERGVKYRDAAKTSIIPVKNIDPNQDLLKKPEWMKIKLPASSAKIESIKNGMRR
CCCCCCHHCCCCHHHHCCCCEEECCCCCCCHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
HGLHSVCEEASCPNLHECFNHGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPQKLAE
HHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
TIQDMKLKYVVITSVDRDDLPDRGAGHFSECVKAVRELNPNIKIEILVPDFRGRVTQALE
HHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
KLKDNPPDVFNHNLENVPRLYKEIRPGADYGWSLKLLREFKEMFPNIPTKSGLMVGLGET
HHHCCCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCC
NEEILQVMQDLRDNGVTMLTLGQYLQPSRHHLPVARYVPPTEFDEFRDKANEMGFEHAAC
HHHHHHHHHHHHHCCEEEEECHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCHHHC
GPFVRSSYHADLQASGGLVK
CHHHHHCCCCCHHCCCCCCC
>Mature Secondary Structure 
STPFKMERGVKYRDAAKTSIIPVKNIDPNQDLLKKPEWMKIKLPASSAKIESIKNGMRR
CCCCCHHCCCCHHHHCCCCEEECCCCCCCHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
HGLHSVCEEASCPNLHECFNHGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPQKLAE
HHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
TIQDMKLKYVVITSVDRDDLPDRGAGHFSECVKAVRELNPNIKIEILVPDFRGRVTQALE
HHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
KLKDNPPDVFNHNLENVPRLYKEIRPGADYGWSLKLLREFKEMFPNIPTKSGLMVGLGET
HHHCCCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCC
NEEILQVMQDLRDNGVTMLTLGQYLQPSRHHLPVARYVPPTEFDEFRDKANEMGFEHAAC
HHHHHHHHHHHHHCCEEEEECHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCHHHC
GPFVRSSYHADLQASGGLVK
CHHHHHCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA