Click here to switch to the map view.

The map label for this gene is lipB

Identifier: 148825692

GI number: 148825692

Start: 637412

End: 638050

Strand: Direct

Name: lipB

Synonym: CGSHiEE_03175

Alternate gene names: 148825692

Gene position: 637412-638050 (Clockwise)

Preceding gene: 148825691

Following gene: 148825693

Centisome position: 35.16

GC content: 37.87

Gene sequence:

>639_bases
ATGAACAATTCCCTTATCGTTCGTCAGTTAGGATTGCAAGATTATCAAGAAATTTGGCACAAAATGCAGGATTTTACTGA
TACTCGAAATGCTGAAACGCAAGACGAAATTTGGTTAGTACAACATTATCCTGTTTTTACACAAGGGCAAGCAGGTAAAC
CAGAGCATCTTTTACAACGTAGTGAAATCCCTGTGGTGCAATCGGATCGTGGAGGGCAAATTACCTATCACGCACCCGGT
CAGCAAGTGATGTATGTGCTTATTGACATTAAACGCCATAAAAACTTAAATGTACGTCAATTAGTTACCGCACTTGAACA
AACGGTGGTTAAAACTTTGGCTGAATATGGCATTGAAAGCTATCCAAAGCCTGATGCGCCAGGTGTTTATGTGGATGGAA
AAAAGATTTGTTCTCTTGGATTACGTATTCGTCGTGGTTGCTCTTTTCACGGGTTAGCATTGAATATTAATATGGATCTT
AATCCTTTCCATTATATTAATCCTTGTGGGTATGCAGGGCTTGAAATGTGTCAGTTAGCGGATTTTGTTAATCAAGATGA
AGCTGATTGCGATAATGTTTCAGCAAAATTAATTAAACACTTCGCCGATCTTTTAGGCTATAATATAACAACTTTATAA

Upstream 100 bases:

>100_bases
AATTCAGTTTCAATTGATATTATTGCTGAAAACTTTGATCAGGTAGAAACACTCTATAAAGAACTGGCTAAAGTTGAAGG
CGTGAAAATGGTTATTTAGT

Downstream 100 bases:

>100_bases
CATTTTATAAAAACTAAATGCCCAATTTGGGCATTTTTATTTAGGAAAATACTATGTCTACGCCTTTCAAAATGGAACGC
GGTGTAAAATATCGCGATGC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNNSLIVRQLGLQDYQEIWHKMQDFTDTRNAETQDEIWLVQHYPVFTQGQAGKPEHLLQRSEIPVVQSDRGGQITYHAPG
QQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIESYPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDL
NPFHYINPCGYAGLEMCQLADFVNQDEADCDNVSAKLIKHFADLLGYNITTL

Sequences:

>Translated_212_residues
MNNSLIVRQLGLQDYQEIWHKMQDFTDTRNAETQDEIWLVQHYPVFTQGQAGKPEHLLQRSEIPVVQSDRGGQITYHAPG
QQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIESYPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDL
NPFHYINPCGYAGLEMCQLADFVNQDEADCDNVSAKLIKHFADLLGYNITTL
>Mature_212_residues
MNNSLIVRQLGLQDYQEIWHKMQDFTDTRNAETQDEIWLVQHYPVFTQGQAGKPEHLLQRSEIPVVQSDRGGQITYHAPG
QQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIESYPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDL
NPFHYINPCGYAGLEMCQLADFVNQDEADCDNVSAKLIKHFADLLGYNITTL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=179, Percent_Identity=31.2849162011173, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI87081767, Length=203, Percent_Identity=65.5172413793103, Blast_Score=281, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6323268, Length=189, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355483, Length=180, Percent_Identity=31.6666666666667, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_HAEI8 (Q4QPL7)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_247690.1
- ProteinModelPortal:   Q4QPL7
- SMR:   Q4QPL7
- STRING:   Q4QPL7
- GeneID:   3431070
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0034
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   GDEIWVV
- PhylomeDB:   Q4QPL7
- ProtClustDB:   PRK14342
- BioCyc:   HINF281310:NTHI0034-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24076; Mature: 24076

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 169-169

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSLIVRQLGLQDYQEIWHKMQDFTDTRNAETQDEIWLVQHYPVFTQGQAGKPEHLLQR
CCCCEEEHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCCCCHHHHHHH
SEIPVVQSDRGGQITYHAPGQQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIES
CCCCEEECCCCCEEEEECCCHHEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
YPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDLNPFHYINPCGYAGLEMCQLA
CCCCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHHH
DFVNQDEADCDNVSAKLIKHFADLLGYNITTL
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNNSLIVRQLGLQDYQEIWHKMQDFTDTRNAETQDEIWLVQHYPVFTQGQAGKPEHLLQR
CCCCEEEHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCCCCHHHHHHH
SEIPVVQSDRGGQITYHAPGQQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIES
CCCCEEECCCCCEEEEECCCHHEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
YPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDLNPFHYINPCGYAGLEMCQLA
CCCCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHHH
DFVNQDEADCDNVSAKLIKHFADLLGYNITTL
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA