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The map label for this gene is gyrB [H]

Identifier: 148825154

GI number: 148825154

Start: 26511

End: 28931

Strand: Direct

Name: gyrB [H]

Synonym: CGSHiEE_00130

Alternate gene names: 148825154

Gene position: 26511-28931 (Clockwise)

Preceding gene: 148825153

Following gene: 148825155

Centisome position: 1.46

GC content: 40.81

Gene sequence:

>2421_bases
ATGTCAGAAACGACCAATGATAACTATGGTGCATCGAGCATTAAAGTATTAAAAGGCCTTGATGCAGTGCGTAAGCGTCC
CGGTATGTATATTGGCGATACCGATGACGGTACTGGTTTGCACCATATGGTATTTGAAGTGGTGGATAATGCCATTGATG
AAGCCCTCGCTGGCCATTGTTCCGATATTATCGTGACAATTCACGATGACAATTCTGTGTCGGTGCAAGATGATGGGCGT
GGAATTCCTGTGGATATTCATCCTGAAGAAGGCGTTTCTGCAGCAGAAGTGATTATGACCGTGCTTCACGCTGGCGGTAA
ATTTGACGATAACTCTTATAAAGTATCGGGCGGTTTACACGGCGTGGGCGTATCTGTAGTGAATGCGCTTTCTGATAAAT
TACAATTAACCATTCGTCGTCAAGGTCACGTTCACGAGCAGTTTTATCATTTAGGGGAGCCACAAGCACCATTAACTGTA
ATTGGTAATACAGAAACAACAGGTACAACAGTGCGTTTCTGGCCAAGCTCAGATATTTTTGCTATCACAACCTTTGATTA
CAAAATCTTAGCAAAACGCTTACGTGAGCTTTCTTTCTTGAACTCTGGTGTATCTATTCGTTTAATCGATAAACGTGATG
GCGCTGAAGATCATTTCCATTACGAAGGCGGTATTCAAGCATTCGTCGAATATTTGAACAAAAATAAAAACCCAATTCAT
CCTAAACCATTTTATTTTACCGCTGAGAAAGATGGCATTGGCGTAGAAGTTGCATTGCAATGGAATGATGGTGTAAATGA
AAATGTGTACTGCTTCACTAATAACATTCCTCAACGTGATGGTGGTACTCACTTAGCAGGTTTCCGTGGGGCTTTAACCC
GTAGCTTAAATAGCTATATGGAAAACGAAGGTATGCTGAAAAAAGAAAAAGTGGCTACTTCAGGCGATGATGCGCGTGAA
GGTTTGGTTGCAATTATTTCAGTCAAAGTGCCTGATCCAAAATTCTCATCGCAAACTAAAGACAAGCTTGTGTCTTCAGA
AGTTAAAAGTGCGGTGGAATCTGCCATGAATGAAAAAATGCAGGAATATTTATTGGAAAATCCTGCAGATGCAAAAATCA
TTGTAAATCAAATTATTATGGCTGCTCGTGCGCGCGAAGCAGCACGCAAAGCTCGTGAAATGACGCGCCGTAAAGGGGTA
TTAGATATTGCAGGTTTACCAGGTAAACTTGCAGACTGCCAAGAAAAAGATCCTGCACTCTCTGAACTTTACTTGGTGGA
GGGGGACTCTGCAGGTGGTTCTGCAAAAGTTGGTCGTGATCGTAAAACTCAAGCAATTTTGCCATTGAAAGGTAAAATCT
TAAACGTAGAAAAAGCACGTTTTGACAAAATGTTGTCTTCCCAAGAGGTGGGGACATTGATTACTGCCTTAGGCTGCGGT
ATCGGTCGTGATGAATATAACCCAGATAAATTACGCTATCATCATATCATTATCATGACCGATGCTGATGTGGATGGTTC
TCACATTCGTACGTTGCTTTTAACGTTTTTCTATCGTCAAATGCCTGAGCTTATTGAGCGTGGTTATGTTTACATTGCTC
AGCCGCCACTTTATAAAGTCAAAAAAGGAAAACAAGAACGCTATATTAAAGATGCGGATGAAATGGAGCAATATGAATTA
ACCCTTGCTTTAGATGGCGCAGAATTACATATTAGCGCAAATGCACCAGCAATGAATGCCCTTGTATTTGAAAAATTGGT
AGCAGAATATAACAGCGTACAAAAACTTATCGGTCGTTTAAGCCGCCATTATCCAGCCCCTGTATTGAAAGGATTAATTT
ATCAGTCGCCAATTTCTGTTGAAATGATGAAAGATGAAAGTGCGGTTGAAAATTGGGGTAAATCTTTTGTTGAACAACTG
ACTGCAAAAGAAACAGAAGCGCATCAATATTCAGTGCGTACACAATTTAACGCGGAACGCCAAGTGTATGAAGCGGTTAT
TACTGTTCGTAAACACGGTATTGATACCGATTATTTCTTGAATTTTGATTTTGTTCACGGCAATGAATACGCGAAAATTG
TTTCTCTTAATAAACAACTCAATGGTTTATTAGAGGAGGGTGCTTATGTTATTCGTGGCGAAAAAGTACAACCAGTGCGT
ACTTTTGAACAAGCCGTTGAATGGCTAGTGAAAGAATCTCGTAAAGGTCTTGAAGTACAACGTTATAAAGGACTTGGGGA
AATGAACGCAGATCAACTTTGGGAAACCACAATGGATCCAAATTCACGTCGTATGTTGAAAGTTTCTATTAAGGATGCCG
TTGCTGCGGATCAACTCTTCACGACATTAATGGGCGATGAAGTTGAGCCACGTCGCGAGTTCATTGAGCTGAATGCGTTG
AGAGCAAATTTGGACGTGTAA

Upstream 100 bases:

>100_bases
CCTTTTTTTATATCTTTCCTTTCCCGAAAAGCATCGCCAAAACGGCGATTTTTTGCTATAATCCCGCCCAATTTTTATTT
ACAAAAGAATGAGATAAATT

Downstream 100 bases:

>100_bases
ATTTTCGTCTATTTATATTCATAATTGAAAGGGCATTTAGATGCCCTTTCTTCGTTTATCTTGTTATTTCATTTCAGGAT
GGCTATTGGTTTTTTGAACT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 806; Mature: 805

Protein sequence:

>806_residues
MSETTNDNYGASSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCSDIIVTIHDDNSVSVQDDGR
GIPVDIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSDKLQLTIRRQGHVHEQFYHLGEPQAPLTV
IGNTETTGTTVRFWPSSDIFAITTFDYKILAKRLRELSFLNSGVSIRLIDKRDGAEDHFHYEGGIQAFVEYLNKNKNPIH
PKPFYFTAEKDGIGVEVALQWNDGVNENVYCFTNNIPQRDGGTHLAGFRGALTRSLNSYMENEGMLKKEKVATSGDDARE
GLVAIISVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKMQEYLLENPADAKIIVNQIIMAARAREAARKAREMTRRKGV
LDIAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKVGRDRKTQAILPLKGKILNVEKARFDKMLSSQEVGTLITALGCG
IGRDEYNPDKLRYHHIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKVKKGKQERYIKDADEMEQYEL
TLALDGAELHISANAPAMNALVFEKLVAEYNSVQKLIGRLSRHYPAPVLKGLIYQSPISVEMMKDESAVENWGKSFVEQL
TAKETEAHQYSVRTQFNAERQVYEAVITVRKHGIDTDYFLNFDFVHGNEYAKIVSLNKQLNGLLEEGAYVIRGEKVQPVR
TFEQAVEWLVKESRKGLEVQRYKGLGEMNADQLWETTMDPNSRRMLKVSIKDAVAADQLFTTLMGDEVEPRREFIELNAL
RANLDV

Sequences:

>Translated_806_residues
MSETTNDNYGASSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCSDIIVTIHDDNSVSVQDDGR
GIPVDIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSDKLQLTIRRQGHVHEQFYHLGEPQAPLTV
IGNTETTGTTVRFWPSSDIFAITTFDYKILAKRLRELSFLNSGVSIRLIDKRDGAEDHFHYEGGIQAFVEYLNKNKNPIH
PKPFYFTAEKDGIGVEVALQWNDGVNENVYCFTNNIPQRDGGTHLAGFRGALTRSLNSYMENEGMLKKEKVATSGDDARE
GLVAIISVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKMQEYLLENPADAKIIVNQIIMAARAREAARKAREMTRRKGV
LDIAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKVGRDRKTQAILPLKGKILNVEKARFDKMLSSQEVGTLITALGCG
IGRDEYNPDKLRYHHIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKVKKGKQERYIKDADEMEQYEL
TLALDGAELHISANAPAMNALVFEKLVAEYNSVQKLIGRLSRHYPAPVLKGLIYQSPISVEMMKDESAVENWGKSFVEQL
TAKETEAHQYSVRTQFNAERQVYEAVITVRKHGIDTDYFLNFDFVHGNEYAKIVSLNKQLNGLLEEGAYVIRGEKVQPVR
TFEQAVEWLVKESRKGLEVQRYKGLGEMNADQLWETTMDPNSRRMLKVSIKDAVAADQLFTTLMGDEVEPRREFIELNAL
RANLDV
>Mature_805_residues
SETTNDNYGASSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCSDIIVTIHDDNSVSVQDDGRG
IPVDIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSDKLQLTIRRQGHVHEQFYHLGEPQAPLTVI
GNTETTGTTVRFWPSSDIFAITTFDYKILAKRLRELSFLNSGVSIRLIDKRDGAEDHFHYEGGIQAFVEYLNKNKNPIHP
KPFYFTAEKDGIGVEVALQWNDGVNENVYCFTNNIPQRDGGTHLAGFRGALTRSLNSYMENEGMLKKEKVATSGDDAREG
LVAIISVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKMQEYLLENPADAKIIVNQIIMAARAREAARKAREMTRRKGVL
DIAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKVGRDRKTQAILPLKGKILNVEKARFDKMLSSQEVGTLITALGCGI
GRDEYNPDKLRYHHIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKVKKGKQERYIKDADEMEQYELT
LALDGAELHISANAPAMNALVFEKLVAEYNSVQKLIGRLSRHYPAPVLKGLIYQSPISVEMMKDESAVENWGKSFVEQLT
AKETEAHQYSVRTQFNAERQVYEAVITVRKHGIDTDYFLNFDFVHGNEYAKIVSLNKQLNGLLEEGAYVIRGEKVQPVRT
FEQAVEWLVKESRKGLEVQRYKGLGEMNADQLWETTMDPNSRRMLKVSIKDAVAADQLFTTLMGDEVEPRREFIELNALR
ANLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=604, Percent_Identity=25.1655629139073, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI19913406, Length=560, Percent_Identity=25.3571428571429, Blast_Score=129, Evalue=8e-30,
Organism=Escherichia coli, GI48994957, Length=803, Percent_Identity=75.2179327521793, Blast_Score=1248, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=554, Percent_Identity=41.5162454873646, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17535065, Length=539, Percent_Identity=25.974025974026, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI212645845, Length=513, Percent_Identity=23.7816764132554, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=155, Percent_Identity=34.8387096774194, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=574, Percent_Identity=25.2613240418118, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17136538, Length=543, Percent_Identity=25.9668508287293, Blast_Score=137, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89925; Mature: 89794

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETTNDNYGASSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHC
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SDIIVTIHDDNSVSVQDDGRGIPVDIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLH
CEEEEEEECCCEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCC
GVGVSVVNALSDKLQLTIRRQGHVHEQFYHLGEPQAPLTVIGNTETTGTTVRFWPSSDIF
HHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCCCEE
AITTFDYKILAKRLRELSFLNSGVSIRLIDKRDGAEDHFHYEGGIQAFVEYLNKNKNPIH
EEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHCCCCCCC
PKPFYFTAEKDGIGVEVALQWNDGVNENVYCFTNNIPQRDGGTHLAGFRGALTRSLNSYM
CCCEEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
ENEGMLKKEKVATSGDDAREGLVAIISVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKM
HCCCCCCHHHCCCCCCCHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QEYLLENPADAKIIVNQIIMAARAREAARKAREMTRRKGVLDIAGLPGKLADCQEKDPAL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCC
SELYLVEGDSAGGSAKVGRDRKTQAILPLKGKILNVEKARFDKMLSSQEVGTLITALGCG
EEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCC
IGRDEYNPDKLRYHHIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKV
CCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHH
KKGKQERYIKDADEMEQYELTLALDGAELHISANAPAMNALVFEKLVAEYNSVQKLIGRL
HCCCHHHHHCCHHHHHHEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
SRHYPAPVLKGLIYQSPISVEMMKDESAVENWGKSFVEQLTAKETEAHQYSVRTQFNAER
HHCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHH
QVYEAVITVRKHGIDTDYFLNFDFVHGNEYAKIVSLNKQLNGLLEEGAYVIRGEKVQPVR
HHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEEHHHHHHHHHHCCEEEEECCCCCHHH
TFEQAVEWLVKESRKGLEVQRYKGLGEMNADQLWETTMDPNSRRMLKVSIKDAVAADQLF
HHHHHHHHHHHHHHCCCEEHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHH
TTLMGDEVEPRREFIELNALRANLDV
HHHHCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SETTNDNYGASSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHC
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SDIIVTIHDDNSVSVQDDGRGIPVDIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLH
CEEEEEEECCCEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCC
GVGVSVVNALSDKLQLTIRRQGHVHEQFYHLGEPQAPLTVIGNTETTGTTVRFWPSSDIF
HHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCCCEE
AITTFDYKILAKRLRELSFLNSGVSIRLIDKRDGAEDHFHYEGGIQAFVEYLNKNKNPIH
EEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHCCCCCCC
PKPFYFTAEKDGIGVEVALQWNDGVNENVYCFTNNIPQRDGGTHLAGFRGALTRSLNSYM
CCCEEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
ENEGMLKKEKVATSGDDAREGLVAIISVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKM
HCCCCCCHHHCCCCCCCHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QEYLLENPADAKIIVNQIIMAARAREAARKAREMTRRKGVLDIAGLPGKLADCQEKDPAL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCC
SELYLVEGDSAGGSAKVGRDRKTQAILPLKGKILNVEKARFDKMLSSQEVGTLITALGCG
EEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCC
IGRDEYNPDKLRYHHIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYVYIAQPPLYKV
CCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHH
KKGKQERYIKDADEMEQYELTLALDGAELHISANAPAMNALVFEKLVAEYNSVQKLIGRL
HCCCHHHHHCCHHHHHHEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
SRHYPAPVLKGLIYQSPISVEMMKDESAVENWGKSFVEQLTAKETEAHQYSVRTQFNAER
HHCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHH
QVYEAVITVRKHGIDTDYFLNFDFVHGNEYAKIVSLNKQLNGLLEEGAYVIRGEKVQPVR
HHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEEHHHHHHHHHHCCEEEEECCCCCHHH
TFEQAVEWLVKESRKGLEVQRYKGLGEMNADQLWETTMDPNSRRMLKVSIKDAVAADQLF
HHHHHHHHHHHHHHCCCEEHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHH
TTLMGDEVEPRREFIELNALRANLDV
HHHHCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]