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The map label for this gene is desA3

Identifier: 148824431

GI number: 148824431

Start: 3617529

End: 3618812

Strand: Reverse

Name: desA3

Synonym: TBFG_13258

Alternate gene names: 148824431

Gene position: 3618812-3617529 (Counterclockwise)

Preceding gene: 148824432

Following gene: 148824428

Centisome position: 81.79

GC content: 61.84

Gene sequence:

>1284_bases
ATGGCGATCACTGACGTCGACGTATTCGCGCATCTGACGGACGCCGACATTGAAAACCTGGCCGCCGAGCTAGATGCCAT
CCGCCGAGACGTTGAAGAATCGCGCGGCGAACGCGATGCCCGCTACATCCGCCGCACCATCGCCGCACAGCGGGCGCTCG
AGGTGTCCGGTCGGCTACTGCTGGCTGGCAGCTCACGACGCCTCGCCTGGTGGACAGGTGCCCTGACCCTTGGCGTGGCC
AAGATCATCGAAAACATGGAGATCGGCCACAACGTCATGCACGGCCAGTGGGACTGGATGAACGACCCGGAGATTCACTC
CTCGACGTGGGAGTGGGACATGAGCGGATCGTCCAAGCACTGGCGCTACACCCACAACTTCGTGCACCACAAATACACCA
ACATCCTCGGTATGGATGACGACGTCGGATACGGCATGCTGCGCGTCACCCGTGACCAGCGCTGGAAGCGCTACAACATC
TTCAACGTGGTGTGGAACACCATCCTTGCGATCGGCTTCGAGTGGGGAGTTGCGCTGCAGCACTTGGAGATCGGCAAGAT
CTTCAAGGGTCGGGCCGACCGCGAGGCCGCTAAAACCCGGTTGCGTGAATTCTCAGCCAAAGCGGGCCGCCAGGTGTTCA
AGGACTATGTCGCGTTCCCGGCGCTGACCTCGTTGTCCCCGGGGGCGACGTATCGGTCCACTTTGACCGCCAACGTGGTG
GCCAACGTGATCCGCAACGTGTGGTCCAACGCGGTGATCTTCTGCGGCCATTTCCCGGATGGCGCAGAGAAATTCACCAA
GACGGACATGATCGGTGAGCCGAAGGGCCAGTGGTACCTACGGCAGATGCTGGGCAGCGCAAACTTCAACGCTGGGCCGG
CGCTGCGGTTCATGAGCGGCAATCTGTGCCACCAAATCGAGCATCACCTCTATCCGGACCTGCCGAGCAACCGGCTTCAC
GAGATTTCGGTGCGGGTGCGTGAGGTCTGCGACAGATACGACTTGCCATACACCACGGGCTCGTTCTTGGTGCAGTACGG
CAAGACCTGGCGCACCCTTGCCAAGCTGTCGTTGCCGGATAAGTACTTACGTGACAACGCTGACGATGCACCCGAGACCC
GCAGTGAGCGGATGTTCGCTGGTCTGGGGCCGGGTTTCGCGGGCGCCGATCCGGTGACCGGACGTCGTCGGGGGTTAAAG
ACCGCGATTGCCGCCGTGCGGGGTCGGCGGCGCAGCAAGCGTATGGCTAAGTCGGTCACCGAACCCGACGATCTGGCAGC
CTAA

Upstream 100 bases:

>100_bases
GTGATTGCGTGCTGGACATCTAGTGTTACTCATAGGTAACCTACGGAATCGTAAGTTACGATAGCGTAGGTCATCGGCCG
TATGACGCAGGGAGAAGACG

Downstream 100 bases:

>100_bases
GATTGCTCGGCGCGCGACGAGCATGCGGCTAGTCAAGTCTGGCTGAGCACTGCCAGTAGTCGCCGGGCGGCCGCGGCGCG
GCGGGCAGCGGGCCCGGACA

Product: linoleoyl-CoA desaturase

Products: NA

Alternate protein names: Delta(9)-desaturase; Stearoyl-CoA desaturase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MAITDVDVFAHLTDADIENLAAELDAIRRDVEESRGERDARYIRRTIAAQRALEVSGRLLLAGSSRRLAWWTGALTLGVA
KIIENMEIGHNVMHGQWDWMNDPEIHSSTWEWDMSGSSKHWRYTHNFVHHKYTNILGMDDDVGYGMLRVTRDQRWKRYNI
FNVVWNTILAIGFEWGVALQHLEIGKIFKGRADREAAKTRLREFSAKAGRQVFKDYVAFPALTSLSPGATYRSTLTANVV
ANVIRNVWSNAVIFCGHFPDGAEKFTKTDMIGEPKGQWYLRQMLGSANFNAGPALRFMSGNLCHQIEHHLYPDLPSNRLH
EISVRVREVCDRYDLPYTTGSFLVQYGKTWRTLAKLSLPDKYLRDNADDAPETRSERMFAGLGPGFAGADPVTGRRRGLK
TAIAAVRGRRRSKRMAKSVTEPDDLAA

Sequences:

>Translated_427_residues
MAITDVDVFAHLTDADIENLAAELDAIRRDVEESRGERDARYIRRTIAAQRALEVSGRLLLAGSSRRLAWWTGALTLGVA
KIIENMEIGHNVMHGQWDWMNDPEIHSSTWEWDMSGSSKHWRYTHNFVHHKYTNILGMDDDVGYGMLRVTRDQRWKRYNI
FNVVWNTILAIGFEWGVALQHLEIGKIFKGRADREAAKTRLREFSAKAGRQVFKDYVAFPALTSLSPGATYRSTLTANVV
ANVIRNVWSNAVIFCGHFPDGAEKFTKTDMIGEPKGQWYLRQMLGSANFNAGPALRFMSGNLCHQIEHHLYPDLPSNRLH
EISVRVREVCDRYDLPYTTGSFLVQYGKTWRTLAKLSLPDKYLRDNADDAPETRSERMFAGLGPGFAGADPVTGRRRGLK
TAIAAVRGRRRSKRMAKSVTEPDDLAA
>Mature_426_residues
AITDVDVFAHLTDADIENLAAELDAIRRDVEESRGERDARYIRRTIAAQRALEVSGRLLLAGSSRRLAWWTGALTLGVAK
IIENMEIGHNVMHGQWDWMNDPEIHSSTWEWDMSGSSKHWRYTHNFVHHKYTNILGMDDDVGYGMLRVTRDQRWKRYNIF
NVVWNTILAIGFEWGVALQHLEIGKIFKGRADREAAKTRLREFSAKAGRQVFKDYVAFPALTSLSPGATYRSTLTANVVA
NVIRNVWSNAVIFCGHFPDGAEKFTKTDMIGEPKGQWYLRQMLGSANFNAGPALRFMSGNLCHQIEHHLYPDLPSNRLHE
ISVRVREVCDRYDLPYTTGSFLVQYGKTWRTLAKLSLPDKYLRDNADDAPETRSERMFAGLGPGFAGADPVTGRRRGLKT
AIAAVRGRRRSKRMAKSVTEPDDLAA

Specific function: Desaturates stearoyl-CoA to form oleoyl-CoA

COG id: COG3239

COG function: function code I; Fatty acid desaturase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Probable). Note=Has been shown to be membrane-associated but no reliable trans-membrane regions are predicted by bioinformatics tools

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fatty acid desaturase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DESA3_MYCTU (Q7D5W1)

Other databases:

- EMBL:   BX842582
- EMBL:   AE000516
- PIR:   G70590
- RefSeq:   NP_337855.1
- RefSeq:   YP_177948.1
- EnsemblBacteria:   EBMYCT00000003542
- EnsemblBacteria:   EBMYCT00000071657
- GeneID:   888821
- GeneID:   922805
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3326
- KEGG:   mtu:Rv3229c
- TIGR:   MT3326
- TubercuList:   Rv3229c
- GeneTree:   EBGT00050000016489
- HOGENOM:   HBG289845
- OMA:   HSSSWEW
- ProtClustDB:   CLSK868967
- InterPro:   IPR005804

Pfam domain/function: PF00487 FA_desaturase

EC number: NA

Molecular weight: Translated: 48444; Mature: 48312

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITDVDVFAHLTDADIENLAAELDAIRRDVEESRGERDARYIRRTIAAQRALEVSGRLL
CCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEE
LAGSSRRLAWWTGALTLGVAKIIENMEIGHNVMHGQWDWMNDPEIHSSTWEWDMSGSSKH
EECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
WRYTHNFVHHKYTNILGMDDDVGYGMLRVTRDQRWKRYNIFNVVWNTILAIGFEWGVALQ
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLEIGKIFKGRADREAAKTRLREFSAKAGRQVFKDYVAFPALTSLSPGATYRSTLTANVV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ANVIRNVWSNAVIFCGHFPDGAEKFTKTDMIGEPKGQWYLRQMLGSANFNAGPALRFMSG
HHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCC
NLCHQIEHHLYPDLPSNRLHEISVRVREVCDRYDLPYTTGSFLVQYGKTWRTLAKLSLPD
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCH
KYLRDNADDAPETRSERMFAGLGPGFAGADPVTGRRRGLKTAIAAVRGRRRSKRMAKSVT
HHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
EPDDLAA
CCHHCCC
>Mature Secondary Structure 
AITDVDVFAHLTDADIENLAAELDAIRRDVEESRGERDARYIRRTIAAQRALEVSGRLL
CCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEE
LAGSSRRLAWWTGALTLGVAKIIENMEIGHNVMHGQWDWMNDPEIHSSTWEWDMSGSSKH
EECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
WRYTHNFVHHKYTNILGMDDDVGYGMLRVTRDQRWKRYNIFNVVWNTILAIGFEWGVALQ
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLEIGKIFKGRADREAAKTRLREFSAKAGRQVFKDYVAFPALTSLSPGATYRSTLTANVV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ANVIRNVWSNAVIFCGHFPDGAEKFTKTDMIGEPKGQWYLRQMLGSANFNAGPALRFMSG
HHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCC
NLCHQIEHHLYPDLPSNRLHEISVRVREVCDRYDLPYTTGSFLVQYGKTWRTLAKLSLPD
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCH
KYLRDNADDAPETRSERMFAGLGPGFAGADPVTGRRRGLKTAIAAVRGRRRSKRMAKSVT
HHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
EPDDLAA
CCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036