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The map label for this gene is hisS

Identifier: 148823775

GI number: 148823775

Start: 2918138

End: 2919409

Strand: Reverse

Name: hisS

Synonym: TBFG_12600

Alternate gene names: 148823775

Gene position: 2919409-2918138 (Counterclockwise)

Preceding gene: 148823777

Following gene: 148823773

Centisome position: 65.98

GC content: 67.3

Gene sequence:

>1272_bases
GTGACGGAATTCTCGTCATTTTCGGCCCCCAAGGGGGTACCGGACTACGTCCCGCCCGACTCGGCGCAGTTCGTCGCGGT
GCGCGACGGGCTGCTCGCGGCGGCCCGTCAAGCCGGCTATAGCCACATCGAGCTGCCCATCTTCGAGGACACCGCCCTGT
TCGCCCGGGGCGTGGGTGAATCCACCGACGTGGTGTCCAAGGAGATGTATACGTTCGCCGACCGTGGCGACCGCTCGGTG
ACGCTGCGGCCCGAGGGCACCGCCGGGGTGGTGCGTGCGGTGATCGAACACGGGCTGGATCGCGGCGCGCTGCCGGTGAA
GTTGTGTTATGCGGGCCCGTTTTTCCGCTACGAGCGTCCGCAGGCCGGCCGGTATCGCCAGTTACAGCAAGTCGGGGTGG
AGGCGATCGGCGTCGACGACCCGGCGTTGGACGCCGAGGTGATCGCCATTGCCGACGCCGGGTTCCGCTCGTTGGGTCTC
GACGGGTTCCGGCTGGAAATCACCTCCCTGGGAGACGAGAGTTGCCGTCCGCAGTACCGGGAACTGTTGCAGGAGTTCTT
GTTTGGACTCGATCTCGACGAGGACACCCGCAGGCGCGCAGGGATCAATCCGCTGCGGGTGCTCGACGACAAGCGACCCG
AATTGCGTGCGATGACGGCGTCGGCGCCGGTGTTGCTGGATCATCTGTCTGATGTCGCCAAGCAGCATTTCGACACCGTG
CTCGCCCATCTGGACGCGCTTGGAGTGCCCTATGTCATCAACCCGCGCATGGTGCGCGGCCTGGACTACTACACCAAGAC
CGCCTTCGAGTTCGTCCATGACGGGCTTGGTGCGCAATCGGGGATCGGCGGCGGGGGGCGCTACGACGGCCTGATGCACC
AGCTTGGCGGGCAGGACTTGTCGGGCATCGGGTTCGGGCTGGGCGTGGACCGGACCGTGCTGGCGCTGCGGGCCGAGGGC
AAGACGGCGGGGGACAGCGCCCGGTGCGACGTGTTCGGCGTGCCGCTTGGCGAGGCGGCCAAGCTCAGGCTGGCGGTGCT
GGCTGGACGACTGCGCGCGGCCGGGGTGCGGGTTGACCTTGCCTATGGTGATCGCGGGCTCAAAGGCGCGATGCGCGCGG
CCGCTCGTTCCGGCGCCCGTGTTGCGTTGGTAGCGGGCGACCGCGACATCGAGGCCGGGACGGTCGCAGTGAAGGACTTG
ACGACGGGTGAGCAAGTTTCGGTCTCGATGGATTCGGTTGTGGCCGAAGTAATTTCGCGGCTGGCTGGGTAG

Upstream 100 bases:

>100_bases
TGGTGCTCGACGACAGCACGGTGGTGCTGCCCGGGCACGGCAACTCCACCACCATTGGGGCCGAGCGGCGCTTCAATCCG
TTCCTTGAGGGCTTGAGCCG

Downstream 100 bases:

>100_bases
TCGCCGCTTCCAGCCGGAGTCACCGAAATCACTTGCGCCACCGTGGCCGCAGGGCCTCAAGGCGGCCATTCTTGTCGGTG
GGCGTCATACGTTTCAAACA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTEFSSFSAPKGVPDYVPPDSAQFVAVRDGLLAAARQAGYSHIELPIFEDTALFARGVGESTDVVSKEMYTFADRGDRSV
TLRPEGTAGVVRAVIEHGLDRGALPVKLCYAGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEVIAIADAGFRSLGL
DGFRLEITSLGDESCRPQYRELLQEFLFGLDLDEDTRRRAGINPLRVLDDKRPELRAMTASAPVLLDHLSDVAKQHFDTV
LAHLDALGVPYVINPRMVRGLDYYTKTAFEFVHDGLGAQSGIGGGGRYDGLMHQLGGQDLSGIGFGLGVDRTVLALRAEG
KTAGDSARCDVFGVPLGEAAKLRLAVLAGRLRAAGVRVDLAYGDRGLKGAMRAAARSGARVALVAGDRDIEAGTVAVKDL
TTGEQVSVSMDSVVAEVISRLAG

Sequences:

>Translated_423_residues
MTEFSSFSAPKGVPDYVPPDSAQFVAVRDGLLAAARQAGYSHIELPIFEDTALFARGVGESTDVVSKEMYTFADRGDRSV
TLRPEGTAGVVRAVIEHGLDRGALPVKLCYAGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEVIAIADAGFRSLGL
DGFRLEITSLGDESCRPQYRELLQEFLFGLDLDEDTRRRAGINPLRVLDDKRPELRAMTASAPVLLDHLSDVAKQHFDTV
LAHLDALGVPYVINPRMVRGLDYYTKTAFEFVHDGLGAQSGIGGGGRYDGLMHQLGGQDLSGIGFGLGVDRTVLALRAEG
KTAGDSARCDVFGVPLGEAAKLRLAVLAGRLRAAGVRVDLAYGDRGLKGAMRAAARSGARVALVAGDRDIEAGTVAVKDL
TTGEQVSVSMDSVVAEVISRLAG
>Mature_422_residues
TEFSSFSAPKGVPDYVPPDSAQFVAVRDGLLAAARQAGYSHIELPIFEDTALFARGVGESTDVVSKEMYTFADRGDRSVT
LRPEGTAGVVRAVIEHGLDRGALPVKLCYAGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEVIAIADAGFRSLGLD
GFRLEITSLGDESCRPQYRELLQEFLFGLDLDEDTRRRAGINPLRVLDDKRPELRAMTASAPVLLDHLSDVAKQHFDTVL
AHLDALGVPYVINPRMVRGLDYYTKTAFEFVHDGLGAQSGIGGGGRYDGLMHQLGGQDLSGIGFGLGVDRTVLALRAEGK
TAGDSARCDVFGVPLGEAAKLRLAVLAGRLRAAGVRVDLAYGDRGLKGAMRAAARSGARVALVAGDRDIEAGTVAVKDLT
TGEQVSVSMDSVVAEVISRLAG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=468, Percent_Identity=22.4358974358974, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1788861, Length=422, Percent_Identity=43.6018957345972, Blast_Score=333, Evalue=9e-93,
Organism=Caenorhabditis elegans, GI71993693, Length=467, Percent_Identity=22.0556745182013, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71993686, Length=467, Percent_Identity=22.0556745182013, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325290, Length=467, Percent_Identity=22.2698072805139, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_MYCBO (P67484)

Other databases:

- EMBL:   BX248343
- RefSeq:   NP_856257.1
- ProteinModelPortal:   P67484
- SMR:   P67484
- EnsemblBacteria:   EBMYCT00000014486
- GeneID:   1092030
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2611c
- GeneTree:   EBGT00050000017632
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   MBOV233413:MB2611C-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 45150; Mature: 45019

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFSSFSAPKGVPDYVPPDSAQFVAVRDGLLAAARQAGYSHIELPIFEDTALFARGVGE
CCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEEECCHHHHHHCCCC
STDVVSKEMYTFADRGDRSVTLRPEGTAGVVRAVIEHGLDRGALPVKLCYAGPFFRYERP
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
QAGRYRQLQQVGVEAIGVDDPALDAEVIAIADAGFRSLGLDGFRLEITSLGDESCRPQYR
CCHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCHHHCCCCCEEEEEEECCCCCCCHHHH
ELLQEFLFGLDLDEDTRRRAGINPLRVLDDKRPELRAMTASAPVLLDHLSDVAKQHFDTV
HHHHHHHHCCCCCHHHHHHCCCCHHHHCCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHH
LAHLDALGVPYVINPRMVRGLDYYTKTAFEFVHDGLGAQSGIGGGGRYDGLMHQLGGQDL
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
SGIGFGLGVDRTVLALRAEGKTAGDSARCDVFGVPLGEAAKLRLAVLAGRLRAAGVRVDL
CCCEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCEEEEE
AYGDRGLKGAMRAAARSGARVALVAGDRDIEAGTVAVKDLTTGEQVSVSMDSVVAEVISR
EECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHH
LAG
HCC
>Mature Secondary Structure 
TEFSSFSAPKGVPDYVPPDSAQFVAVRDGLLAAARQAGYSHIELPIFEDTALFARGVGE
CCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEEECCHHHHHHCCCC
STDVVSKEMYTFADRGDRSVTLRPEGTAGVVRAVIEHGLDRGALPVKLCYAGPFFRYERP
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
QAGRYRQLQQVGVEAIGVDDPALDAEVIAIADAGFRSLGLDGFRLEITSLGDESCRPQYR
CCHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCHHHCCCCCEEEEEEECCCCCCCHHHH
ELLQEFLFGLDLDEDTRRRAGINPLRVLDDKRPELRAMTASAPVLLDHLSDVAKQHFDTV
HHHHHHHHCCCCCHHHHHHCCCCHHHHCCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHH
LAHLDALGVPYVINPRMVRGLDYYTKTAFEFVHDGLGAQSGIGGGGRYDGLMHQLGGQDL
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
SGIGFGLGVDRTVLALRAEGKTAGDSARCDVFGVPLGEAAKLRLAVLAGRLRAAGVRVDL
CCCEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCEEEEE
AYGDRGLKGAMRAAARSGARVALVAGDRDIEAGTVAVKDLTTGEQVSVSMDSVVAEVISR
EECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHH
LAG
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972