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The map label for this gene is cysS

Identifier: 148663444

GI number: 148663444

Start: 4031060

End: 4032469

Strand: Reverse

Name: cysS

Synonym: MRA_3619

Alternate gene names: 148663444

Gene position: 4032469-4031060 (Counterclockwise)

Preceding gene: 148663445

Following gene: 148663443

Centisome position: 91.23

GC content: 65.89

Gene sequence:

>1410_bases
GTGACCGATCGGGCTCGCTTGCGGCTACACGACACTGCCGCCGGTGTCGTGCGGGATTTCGTCCCGTTGCGACCTGGACA
CGTCTCGATCTACCTGTGCGGTGCCACGGTGCAGGGCCTACCGCATATCGGGCATGTCCGCAGCGGGGTCGCCTTCGACA
TCCTGCGCCGATGGTTGCTCGCGCGTGGCTACGACGTCGCGTTTATCCGCAACGTGACCGACATCGAAGACAAGATCCTG
GCCAAGGCCGCCGCGGCGGGCCGGCCGTGGTGGGAGTGGGCGGCTACCCACGAGCGTGCCTTCACCGCGGCCTACGACGC
TCTGGACGTCTTGCCGCCGTCCGCGGAGCCGCGCGCCACCGGGCATATCACCCAGATGATCGAGATGATCGAGCGCCTGA
TCCAGGCCGGCCACGCGTATACCGGTGGCGGTGACGTTTACTTCGACGTTCTGAGCTACCCGGAGTACGGCCAGTTATCC
GGTCACAAGATCGATGACGTCCATCAGGGCGAAGGTGTGGCTGCCGGCAAGCGGGACCAGCGCGACTTCACTTTGTGGAA
GGGCGAAAAGCCGGGTGAACCGTCGTGGCCGACGCCGTGGGGCCGCGGGCGTCCGGGCTGGCATCTGGAATGCTCGGCAA
TGGCTCGCAGCTATCTCGGGCCGGAATTCGATATCCATTGCGGTGGAATGGATTTAGTCTTCCCGCATCATGAGAACGAA
ATCGCCCAGAGCCGCGCCGCCGGTGATGGGTTCGCCCGCTACTGGCTGCACAATGGCTGGGTGACGATGGGCGGGGAGAA
GATGAGCAAGTCGCTGGGCAACGTGTTGTCCATGCCGGCGATGTTGCAGCGGGTGCGGCCGGCTGAGCTGCGTTATTACC
TGGGCAGCGCGCACTACCGGTCGATGCTCGAGTTCTCCGAGACCGCTATGCAGGATGCGGTGAAAGCCTATGTCGGGCTA
GAGGACTTCCTGCACCGCGTTCGCACCCGGGTGGGCGCCGTCTGCCCCGGCGATCCGACCCCACGGTTCGCCGAAGCGCT
CGACGACGACCTGTCCGTTCCGATCGCGCTCGCCGAGATTCACCACGTGCGGGCGGAAGGCAACCGGGCACTCGATGCCG
GCGACCACGACGGGGCCTTGCGAAGCGCTAGCGCGATTCGGGCGATGATGGGCATCCTGGGCTGTGACCCGCTCGACCAG
CGCTGGGAATCCCGAGACGAAACCTCGGCAGCGCTGGCCGCCGTCGATGTGCTGGTCCAGGCTGAACTACAGAATCGGGA
AAAGGCCCGCGAGCAGCGCAACTGGGCGCTCGCCGACGAGATCCGGGGTCGGCTGAAGAGAGCCGGCATCGAGGTCACCG
ACACCGCCGACGGGCCACAGTGGTCGCTGCTGGGTGGTGACACCAAGTAG

Upstream 100 bases:

>100_bases
GCAATCGCTACGGCATTGGTGGTTTCGTTGCGGTAGCGCACTCCTGGTCCGGACGCGCGCGCCGCAGGTTGCAGCAATTG
GGCAGGTAAGCTGGCACGTC

Downstream 100 bases:

>100_bases
ATGCCCGGTAACTCTCGGCGGCGGGGTGCGGTCCGCAAATCCGGTACCAAGAAAGGCGCAGGCGTCGGCTCGGGCGGTCA
GCGACGCCGTGGGCTGGAAG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTDRARLRLHDTAAGVVRDFVPLRPGHVSIYLCGATVQGLPHIGHVRSGVAFDILRRWLLARGYDVAFIRNVTDIEDKIL
AKAAAAGRPWWEWAATHERAFTAAYDALDVLPPSAEPRATGHITQMIEMIERLIQAGHAYTGGGDVYFDVLSYPEYGQLS
GHKIDDVHQGEGVAAGKRDQRDFTLWKGEKPGEPSWPTPWGRGRPGWHLECSAMARSYLGPEFDIHCGGMDLVFPHHENE
IAQSRAAGDGFARYWLHNGWVTMGGEKMSKSLGNVLSMPAMLQRVRPAELRYYLGSAHYRSMLEFSETAMQDAVKAYVGL
EDFLHRVRTRVGAVCPGDPTPRFAEALDDDLSVPIALAEIHHVRAEGNRALDAGDHDGALRSASAIRAMMGILGCDPLDQ
RWESRDETSAALAAVDVLVQAELQNREKAREQRNWALADEIRGRLKRAGIEVTDTADGPQWSLLGGDTK

Sequences:

>Translated_469_residues
MTDRARLRLHDTAAGVVRDFVPLRPGHVSIYLCGATVQGLPHIGHVRSGVAFDILRRWLLARGYDVAFIRNVTDIEDKIL
AKAAAAGRPWWEWAATHERAFTAAYDALDVLPPSAEPRATGHITQMIEMIERLIQAGHAYTGGGDVYFDVLSYPEYGQLS
GHKIDDVHQGEGVAAGKRDQRDFTLWKGEKPGEPSWPTPWGRGRPGWHLECSAMARSYLGPEFDIHCGGMDLVFPHHENE
IAQSRAAGDGFARYWLHNGWVTMGGEKMSKSLGNVLSMPAMLQRVRPAELRYYLGSAHYRSMLEFSETAMQDAVKAYVGL
EDFLHRVRTRVGAVCPGDPTPRFAEALDDDLSVPIALAEIHHVRAEGNRALDAGDHDGALRSASAIRAMMGILGCDPLDQ
RWESRDETSAALAAVDVLVQAELQNREKAREQRNWALADEIRGRLKRAGIEVTDTADGPQWSLLGGDTK
>Mature_468_residues
TDRARLRLHDTAAGVVRDFVPLRPGHVSIYLCGATVQGLPHIGHVRSGVAFDILRRWLLARGYDVAFIRNVTDIEDKILA
KAAAAGRPWWEWAATHERAFTAAYDALDVLPPSAEPRATGHITQMIEMIERLIQAGHAYTGGGDVYFDVLSYPEYGQLSG
HKIDDVHQGEGVAAGKRDQRDFTLWKGEKPGEPSWPTPWGRGRPGWHLECSAMARSYLGPEFDIHCGGMDLVFPHHENEI
AQSRAAGDGFARYWLHNGWVTMGGEKMSKSLGNVLSMPAMLQRVRPAELRYYLGSAHYRSMLEFSETAMQDAVKAYVGLE
DFLHRVRTRVGAVCPGDPTPRFAEALDDDLSVPIALAEIHHVRAEGNRALDAGDHDGALRSASAIRAMMGILGCDPLDQR
WESRDETSAALAAVDVLVQAELQNREKAREQRNWALADEIRGRLKRAGIEVTDTADGPQWSLLGGDTK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=487, Percent_Identity=35.7289527720739, Blast_Score=222, Evalue=7e-58,
Organism=Homo sapiens, GI10835051, Length=310, Percent_Identity=37.0967741935484, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI62240992, Length=310, Percent_Identity=37.0967741935484, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI21269877, Length=310, Percent_Identity=37.0967741935484, Blast_Score=195, Evalue=9e-50,
Organism=Homo sapiens, GI303304987, Length=310, Percent_Identity=37.0967741935484, Blast_Score=195, Evalue=9e-50,
Organism=Escherichia coli, GI1786737, Length=463, Percent_Identity=42.7645788336933, Blast_Score=330, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17509795, Length=265, Percent_Identity=35.8490566037736, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17509797, Length=265, Percent_Identity=35.8490566037736, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324082, Length=342, Percent_Identity=33.0409356725146, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI19922218, Length=492, Percent_Identity=31.3008130081301, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI19922364, Length=263, Percent_Identity=38.0228136882129, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_MYCBO (P0A635)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857250.1
- ProteinModelPortal:   P0A635
- SMR:   P0A635
- EnsemblBacteria:   EBMYCT00000016757
- GeneID:   1093618
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3611c
- GeneTree:   EBGT00050000016491
- HOGENOM:   HBG327651
- OMA:   PEEMLAG
- ProtClustDB:   PRK00260
- BioCyc:   MBOV233413:MB3611C-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51855; Mature: 51724

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRARLRLHDTAAGVVRDFVPLRPGHVSIYLCGATVQGLPHIGHVRSGVAFDILRRWLL
CCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEEECCHHCCCCCHHHHHCCHHHHHHHHHHH
ARGYDVAFIRNVTDIEDKILAKAAAAGRPWWEWAATHERAFTAAYDALDVLPPSAEPRAT
HCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
GHITQMIEMIERLIQAGHAYTGGGDVYFDVLSYPEYGQLSGHKIDDVHQGEGVAAGKRDQ
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RDFTLWKGEKPGEPSWPTPWGRGRPGWHLECSAMARSYLGPEFDIHCGGMDLVFPHHENE
CCCEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEEEECCEEEECCCCCHH
IAQSRAAGDGFARYWLHNGWVTMGGEKMSKSLGNVLSMPAMLQRVRPAELRYYLGSAHYR
HHHHHCCCCHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SMLEFSETAMQDAVKAYVGLEDFLHRVRTRVGAVCPGDPTPRFAEALDDDLSVPIALAEI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHHH
HHVRAEGNRALDAGDHDGALRSASAIRAMMGILGCDPLDQRWESRDETSAALAAVDVLVQ
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHH
AELQNREKAREQRNWALADEIRGRLKRAGIEVTDTADGPQWSLLGGDTK
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCC
>Mature Secondary Structure 
TDRARLRLHDTAAGVVRDFVPLRPGHVSIYLCGATVQGLPHIGHVRSGVAFDILRRWLL
CCCCEEEEHHHHHHHHHHHHCCCCCEEEEEEECCHHCCCCCHHHHHCCHHHHHHHHHHH
ARGYDVAFIRNVTDIEDKILAKAAAAGRPWWEWAATHERAFTAAYDALDVLPPSAEPRAT
HCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
GHITQMIEMIERLIQAGHAYTGGGDVYFDVLSYPEYGQLSGHKIDDVHQGEGVAAGKRDQ
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RDFTLWKGEKPGEPSWPTPWGRGRPGWHLECSAMARSYLGPEFDIHCGGMDLVFPHHENE
CCCEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEEEECCEEEECCCCCHH
IAQSRAAGDGFARYWLHNGWVTMGGEKMSKSLGNVLSMPAMLQRVRPAELRYYLGSAHYR
HHHHHCCCCHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SMLEFSETAMQDAVKAYVGLEDFLHRVRTRVGAVCPGDPTPRFAEALDDDLSVPIALAEI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHHH
HHVRAEGNRALDAGDHDGALRSASAIRAMMGILGCDPLDQRWESRDETSAALAAVDVLVQ
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHH
AELQNREKAREQRNWALADEIRGRLKRAGIEVTDTADGPQWSLLGGDTK
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972