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The map label for this gene is ispF [H]

Identifier: 148663445

GI number: 148663445

Start: 4032534

End: 4033013

Strand: Reverse

Name: ispF [H]

Synonym: MRA_3620

Alternate gene names: 148663445

Gene position: 4033013-4032534 (Counterclockwise)

Preceding gene: 148663446

Following gene: 148663444

Centisome position: 91.25

GC content: 66.88

Gene sequence:

>480_bases
GTGAATCAGCTGCCCCGCGTTGGTTTGGGGACCGATGTGCATCCGATCGAACCCGGACGTCCGTGCTGGCTGGTAGGGTT
GTTGTTCCCGAGCGCCGACGGTTGCGCGGGCCACTCCGACGGTGACGTGGCCGTTCATGCGCTGTGCGACGCGGTGCTGT
CGGCCGCGGGGCTAGGCGACATTGGCGAGGTGTTCGGCGTCGACGATCCCCGTTGGCAGGGTGTCAGTGGTGCCGACATG
TTGCGCCATGTCGTCGTCCTGATCACCCAGCATGGCTATCGGGTCGGCAACGCTGTGGTGCAGGTGATCGGCAACCGACC
GAAGATCGGTTGGCGCCGGCTCGAGGCGCAGGCGGTGCTGTCACGTCTGCTCAACGCGCCGGTGTCGGTGTCGGCTACCA
CCACCGACGGGCTGGGCCTCACTGGCCGCGGCGAGGGCTTGGCCGCAATCGCTACGGCATTGGTGGTTTCGTTGCGGTAG

Upstream 100 bases:

>100_bases
AGCATATCGGCGGCCAGGTGCAGGTGGTCGACGGCGATCCGCTGGCGTTCAAGATCACCACCAAACTGGATCTGTTGCTG
GCCCAAGCTATAGTGCGCGG

Downstream 100 bases:

>100_bases
CGCACTCCTGGTCCGGACGCGCGCGCCGCAGGTTGCAGCAATTGGGCAGGTAAGCTGGCACGTCGTGACCGATCGGGCTC
GCTTGCGGCTACACGACACT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNQLPRVGLGTDVHPIEPGRPCWLVGLLFPSADGCAGHSDGDVAVHALCDAVLSAAGLGDIGEVFGVDDPRWQGVSGADM
LRHVVVLITQHGYRVGNAVVQVIGNRPKIGWRRLEAQAVLSRLLNAPVSVSATTTDGLGLTGRGEGLAAIATALVVSLR

Sequences:

>Translated_159_residues
MNQLPRVGLGTDVHPIEPGRPCWLVGLLFPSADGCAGHSDGDVAVHALCDAVLSAAGLGDIGEVFGVDDPRWQGVSGADM
LRHVVVLITQHGYRVGNAVVQVIGNRPKIGWRRLEAQAVLSRLLNAPVSVSATTTDGLGLTGRGEGLAAIATALVVSLR
>Mature_159_residues
MNQLPRVGLGTDVHPIEPGRPCWLVGLLFPSADGCAGHSDGDVAVHALCDAVLSAAGLGDIGEVFGVDDPRWQGVSGADM
LRHVVVLITQHGYRVGNAVVQVIGNRPKIGWRRLEAQAVLSRLLNAPVSVSATTTDGLGLTGRGEGLAAIATALVVSLR

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=41.2903225806452, Blast_Score=104, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 16458; Mature: 16458

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLPRVGLGTDVHPIEPGRPCWLVGLLFPSADGCAGHSDGDVAVHALCDAVLSAAGLGD
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
IGEVFGVDDPRWQGVSGADMLRHVVVLITQHGYRVGNAVVQVIGNRPKIGWRRLEAQAVL
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
SRLLNAPVSVSATTTDGLGLTGRGEGLAAIATALVVSLR
HHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNQLPRVGLGTDVHPIEPGRPCWLVGLLFPSADGCAGHSDGDVAVHALCDAVLSAAGLGD
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
IGEVFGVDDPRWQGVSGADMLRHVVVLITQHGYRVGNAVVQVIGNRPKIGWRRLEAQAVL
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
SRLLNAPVSVSATTTDGLGLTGRGEGLAAIATALVVSLR
HHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA