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The map label for this gene is yjfH [C]

Identifier: 148663443

GI number: 148663443

Start: 4030091

End: 4031059

Strand: Reverse

Name: yjfH [C]

Synonym: MRA_3618

Alternate gene names: 148663443

Gene position: 4031059-4030091 (Counterclockwise)

Preceding gene: 148663444

Following gene: 148663439

Centisome position: 91.2

GC content: 68.83

Gene sequence:

>969_bases
ATGCCCGGTAACTCTCGGCGGCGGGGTGCGGTCCGCAAATCCGGTACCAAGAAAGGCGCAGGCGTCGGCTCGGGCGGTCA
GCGACGCCGTGGGCTGGAAGGGCGCGGGCCTACCCCGCCGGCGCACCTGCGTCCCCACCATCCCGCCGCCAAGCGGGCAC
GTGCACAGCCACGCCGCCCGGTCAAGCGCGCGGACGAGACCGAGACGGTGTTGGGTCGCAACCCAGTGCTGGAATGCCTG
CGCGCGGGTGTTCCGGCCACCGCGCTATACGTCGCCCTTGGTACCGAGGCCGACGAGCGACTGACTGAATGCGTTGCGCG
GGCGGCTGATTCAGGGATTGCGATCGTCGAGCTGCTACGTGCCGACCTGGATCGGATGACCGCCAACCACCTCCATCAAG
GCATCGCGCTACAGGTGCCACCGTACAACTACGCCCATCCCGATGACCTGCTGGCCGCCGCCCTCGACCAACCGCCTGCG
CTGCTGGTCGCTTTGGACAACCTTTCCGACCCGCGCAACCTCGGTGCGATCGTGCGGTCGGTGGCCGCGTTCGGTGGCCA
TGGGGTGCTGATTCCGCAACGCCGTTCTGCGTCGGTGACCGCGGTGGCGTGGCGCACCAGCGCCGGGGCGGCGGCCCGGA
TTCCGGTGGCCCGGGCCACCAATCTCACCAGGACGCTGAAGGGCTGGGCCGATCGTGGAGTGCGGGTGATCGGACTGGAC
GCCGGTGGCGGCACCGCGCTCGACGATGTAGATGGCACTGACTCGTTGGTGGTGGTCGTTGGGTCGGAAGGCAAGGGGTT
GTCCCGGTTGGTAAGGCAGAACTGTGACGAGGTGGTGTCCATCCCGATGGCCGCTCAGGCCGAATCACTGAACGCCTCGG
TGGCCGCTGGGGTGGTGCTCGCCGAGATCGCCCGCCAGCGCCGGCGGCCACGCGAGCCTCGCGAGCAGACGCAGAATCGC
ATGATTTGA

Upstream 100 bases:

>100_bases
CGCCGACGAGATCCGGGGTCGGCTGAAGAGAGCCGGCATCGAGGTCACCGACACCGCCGACGGGCCACAGTGGTCGCTGC
TGGGTGGTGACACCAAGTAG

Downstream 100 bases:

>100_bases
GCTCAAATCATGCGATTCTGCGTCTGCTCGCGCCCTTGCGGCTAGATCCCCAGAACCTGGGCGCTGGCCCACAGCGCGAG
CACCGCCATCGCCAGGGCCG

Product: putative tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPGNSRRRGAVRKSGTKKGAGVGSGGQRRRGLEGRGPTPPAHLRPHHPAAKRARAQPRRPVKRADETETVLGRNPVLECL
RAGVPATALYVALGTEADERLTECVARAADSGIAIVELLRADLDRMTANHLHQGIALQVPPYNYAHPDDLLAAALDQPPA
LLVALDNLSDPRNLGAIVRSVAAFGGHGVLIPQRRSASVTAVAWRTSAGAAARIPVARATNLTRTLKGWADRGVRVIGLD
AGGGTALDDVDGTDSLVVVVGSEGKGLSRLVRQNCDEVVSIPMAAQAESLNASVAAGVVLAEIARQRRRPREPREQTQNR
MI

Sequences:

>Translated_322_residues
MPGNSRRRGAVRKSGTKKGAGVGSGGQRRRGLEGRGPTPPAHLRPHHPAAKRARAQPRRPVKRADETETVLGRNPVLECL
RAGVPATALYVALGTEADERLTECVARAADSGIAIVELLRADLDRMTANHLHQGIALQVPPYNYAHPDDLLAAALDQPPA
LLVALDNLSDPRNLGAIVRSVAAFGGHGVLIPQRRSASVTAVAWRTSAGAAARIPVARATNLTRTLKGWADRGVRVIGLD
AGGGTALDDVDGTDSLVVVVGSEGKGLSRLVRQNCDEVVSIPMAAQAESLNASVAAGVVLAEIARQRRRPREPREQTQNR
MI
>Mature_321_residues
PGNSRRRGAVRKSGTKKGAGVGSGGQRRRGLEGRGPTPPAHLRPHHPAAKRARAQPRRPVKRADETETVLGRNPVLECLR
AGVPATALYVALGTEADERLTECVARAADSGIAIVELLRADLDRMTANHLHQGIALQVPPYNYAHPDDLLAAALDQPPAL
LVALDNLSDPRNLGAIVRSVAAFGGHGVLIPQRRSASVTAVAWRTSAGAAARIPVARATNLTRTLKGWADRGVRVIGLDA
GGGTALDDVDGTDSLVVVVGSEGKGLSRLVRQNCDEVVSIPMAAQAESLNASVAAGVVLAEIARQRRRPREPREQTQNRM
I

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=263, Percent_Identity=29.277566539924, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1790623, Length=244, Percent_Identity=38.1147540983607, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788935, Length=247, Percent_Identity=27.5303643724696, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=293, Percent_Identity=30.0341296928328, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 34010; Mature: 33879

Theoretical pI: Translated: 11.36; Mature: 11.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGNSRRRGAVRKSGTKKGAGVGSGGQRRRGLEGRGPTPPAHLRPHHPAAKRARAQPRRP
CCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
VKRADETETVLGRNPVLECLRAGVPATALYVALGTEADERLTECVARAADSGIAIVELLR
HHHCCCHHHHHCCCHHHHHHHCCCCHHHEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHH
ADLDRMTANHLHQGIALQVPPYNYAHPDDLLAAALDQPPALLVALDNLSDPRNLGAIVRS
HHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
VAAFGGHGVLIPQRRSASVTAVAWRTSAGAAARIPVARATNLTRTLKGWADRGVRVIGLD
HHHHCCCEEEEECCCCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEEEE
AGGGTALDDVDGTDSLVVVVGSEGKGLSRLVRQNCDEVVSIPMAAQAESLNASVAAGVVL
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCHHHHHHCCCHHHHHHCCHHHHHHHHH
AEIARQRRRPREPREQTQNRMI
HHHHHHHCCCCCHHHHHHCCCC
>Mature Secondary Structure 
PGNSRRRGAVRKSGTKKGAGVGSGGQRRRGLEGRGPTPPAHLRPHHPAAKRARAQPRRP
CCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
VKRADETETVLGRNPVLECLRAGVPATALYVALGTEADERLTECVARAADSGIAIVELLR
HHHCCCHHHHHCCCHHHHHHHCCCCHHHEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHH
ADLDRMTANHLHQGIALQVPPYNYAHPDDLLAAALDQPPALLVALDNLSDPRNLGAIVRS
HHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
VAAFGGHGVLIPQRRSASVTAVAWRTSAGAAARIPVARATNLTRTLKGWADRGVRVIGLD
HHHHCCCEEEEECCCCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEEEE
AGGGTALDDVDGTDSLVVVVGSEGKGLSRLVRQNCDEVVSIPMAAQAESLNASVAAGVVL
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCHHHHHHCCCHHHHHHCCHHHHHHHHH
AEIARQRRRPREPREQTQNRMI
HHHHHHHCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]