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The map label for this gene is bphC

Identifier: 148663432

GI number: 148663432

Start: 4017963

End: 4018865

Strand: Reverse

Name: bphC

Synonym: MRA_3607

Alternate gene names: 148663432

Gene position: 4018865-4017963 (Counterclockwise)

Preceding gene: 148663433

Following gene: 148663431

Centisome position: 90.93

GC content: 65.12

Gene sequence:

>903_bases
ATGAGCATCCGGTCGCTGGGCTATCTGCGCATCGAGGCCACTGACATGGCTGCCTGGCGGGAGTATGGACTGAAGGTCCT
GGGCATGGTCGAGGGCAAAGGGGCCCCAGAAGGGGCCCTGTATCTGCGGATGGATGACTTTCCGGCCCGGCTGGTGGTCG
TGCCCGGTGAGCACGACAGGCTGTTGGAGGCCGGTTGGGAATGCGCGAATGCCGAAGGCCTGCAGGAAATCCGGAACCGC
CTGGATTTGGAGGGCACGCCGTACAAAGAGGCCACCGCCGCCGAGCTGGCCGATCGCCGGGTGGACGAGATGATCCGGTT
TGCCGACCCTTCCGGCAATTGCCTGGAGGTCTTCCACGGTACCGCGCTGGAACACCGCCGAGTGGTCAGCCCGTATGGGC
ACAGGTTCGTCACCGGTGAGCAGGGTATGGGCCATGTGGTGCTGTCCACCCGCGACGATGCTGAGGCGTTGCATTTCTAC
CGAGACGTGCTCGGCTTTCGGCTGCGTGACTCGATGCGGCTACCGCCACAGATGGTAGGGCGGCCCGCCGACGGGCCGCC
AGCCTGGCTGCGTTTCTTCGGCTGTAACCCGCGTCACCACTCGTTGGCCTTCCTGCCGATGCCGACGTCCAGCGGCATCG
TGCACCTGATGGTCGAGGTCGAGCAGGCCGACGACGTGGGCCTGTGCCTGGACCGGGCGTTGCGGCGCAAGGTGCCGATG
TCGGCCACCCTGGGCCGGCATGTCAACGACCTGATGCTGTCGTTCTACATGAAGACGCCCGGTGGCTTCGACATCGAATT
CGGTTGTGAGGGCAGGCAAGTCGACGATCGGGACTGGATCGCCCGGGAGAGTACTGCGGTCAGCCTATGGGGTCACGATT
TCACCGTAGGCGCTCGCGGCTAG

Upstream 100 bases:

>100_bases
GTGCGCAGCTGCACGTATTCGGGCAGTGTGGGCATTGGGTGCAGGTGGAGAAGTTCGACGAGTTCAACAAGCTGACGATT
GAATTTCTGGGAGGTGGCAG

Downstream 100 bases:

>100_bases
CTCGAAAGCAAACGATGTCGGCTCAGATCGATCCACGCACGTTCCGCTCCGTGCTCGGTCAGTTCTGTACGGGGATCACT
GTCATCACCACTGTGCACGA

Product: extradiol ring-cleavage dioxygenase

Products: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate; H2O

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSIRSLGYLRIEATDMAAWREYGLKVLGMVEGKGAPEGALYLRMDDFPARLVVVPGEHDRLLEAGWECANAEGLQEIRNR
LDLEGTPYKEATAAELADRRVDEMIRFADPSGNCLEVFHGTALEHRRVVSPYGHRFVTGEQGMGHVVLSTRDDAEALHFY
RDVLGFRLRDSMRLPPQMVGRPADGPPAWLRFFGCNPRHHSLAFLPMPTSSGIVHLMVEVEQADDVGLCLDRALRRKVPM
SATLGRHVNDLMLSFYMKTPGGFDIEFGCEGRQVDDRDWIARESTAVSLWGHDFTVGARG

Sequences:

>Translated_300_residues
MSIRSLGYLRIEATDMAAWREYGLKVLGMVEGKGAPEGALYLRMDDFPARLVVVPGEHDRLLEAGWECANAEGLQEIRNR
LDLEGTPYKEATAAELADRRVDEMIRFADPSGNCLEVFHGTALEHRRVVSPYGHRFVTGEQGMGHVVLSTRDDAEALHFY
RDVLGFRLRDSMRLPPQMVGRPADGPPAWLRFFGCNPRHHSLAFLPMPTSSGIVHLMVEVEQADDVGLCLDRALRRKVPM
SATLGRHVNDLMLSFYMKTPGGFDIEFGCEGRQVDDRDWIARESTAVSLWGHDFTVGARG
>Mature_299_residues
SIRSLGYLRIEATDMAAWREYGLKVLGMVEGKGAPEGALYLRMDDFPARLVVVPGEHDRLLEAGWECANAEGLQEIRNRL
DLEGTPYKEATAAELADRRVDEMIRFADPSGNCLEVFHGTALEHRRVVSPYGHRFVTGEQGMGHVVLSTRDDAEALHFYR
DVLGFRLRDSMRLPPQMVGRPADGPPAWLRFFGCNPRHHSLAFLPMPTSSGIVHLMVEVEQADDVGLCLDRALRRKVPMS
ATLGRHVNDLMLSFYMKTPGGFDIEFGCEGRQVDDRDWIARESTAVSLWGHDFTVGARG

Specific function: Catalyzes the meta-cleavage of 3,4-dihydroxy-9,10- seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) to produce 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4- oic acid (4,9-DSHA)

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSAC_MYCTU (P96850)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842583
- PIR:   F70605
- RefSeq:   NP_218085.1
- RefSeq:   NP_338218.1
- PDB:   2ZI8
- PDB:   2ZYQ
- PDBsum:   2ZI8
- PDBsum:   2ZYQ
- ProteinModelPortal:   P96850
- SMR:   P96850
- EnsemblBacteria:   EBMYCT00000000484
- EnsemblBacteria:   EBMYCT00000070288
- GeneID:   887886
- GeneID:   922829
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3673
- KEGG:   mtu:Rv3568c
- TIGR:   MT3673
- TubercuList:   Rv3568c
- GeneTree:   EBGT00050000016127
- HOGENOM:   HBG400175
- OMA:   KMSATLG
- ProtClustDB:   CLSK872204
- InterPro:   IPR004360
- InterPro:   IPR000486

Pfam domain/function: PF00903 Glyoxalase

EC number: 1.13.11.39

Molecular weight: Translated: 33583; Mature: 33452

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00082 EXTRADIOL_DIOXYGENAS

Important sites: BINDING 200-200 BINDING 215-215 BINDING 250-250 BINDING 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRSLGYLRIEATDMAAWREYGLKVLGMVEGKGAPEGALYLRMDDFPARLVVVPGEHDR
CCCCCCEEEEEEEHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCHH
LLEAGWECANAEGLQEIRNRLDLEGTPYKEATAAELADRRVDEMIRFADPSGNCLEVFHG
HHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
TALEHRRVVSPYGHRFVTGEQGMGHVVLSTRDDAEALHFYRDVLGFRLRDSMRLPPQMVG
CCHHHCCCCCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHC
RPADGPPAWLRFFGCNPRHHSLAFLPMPTSSGIVHLMVEVEQADDVGLCLDRALRRKVPM
CCCCCCHHHEEEECCCCCCCEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCC
SATLGRHVNDLMLSFYMKTPGGFDIEFGCEGRQVDDRDWIARESTAVSLWGHDFTVGARG
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHCCCCCEEEECCCEEEECCCC
>Mature Secondary Structure 
SIRSLGYLRIEATDMAAWREYGLKVLGMVEGKGAPEGALYLRMDDFPARLVVVPGEHDR
CCCCCEEEEEEEHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCHH
LLEAGWECANAEGLQEIRNRLDLEGTPYKEATAAELADRRVDEMIRFADPSGNCLEVFHG
HHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
TALEHRRVVSPYGHRFVTGEQGMGHVVLSTRDDAEALHFYRDVLGFRLRDSMRLPPQMVG
CCHHHCCCCCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHC
RPADGPPAWLRFFGCNPRHHSLAFLPMPTSSGIVHLMVEVEQADDVGLCLDRALRRKVPM
CCCCCCHHHEEEECCCCCCCEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCC
SATLGRHVNDLMLSFYMKTPGGFDIEFGCEGRQVDDRDWIARESTAVSLWGHDFTVGARG
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHCCCCCEEEECCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: biphenyl-2,3-diol; O2

Specific reaction: biphenyl-2,3-diol + O2 = 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036