Click here to switch to the map view.

The map label for this gene is bphD

Identifier: 148663433

GI number: 148663433

Start: 4018862

End: 4019737

Strand: Reverse

Name: bphD

Synonym: MRA_3608

Alternate gene names: 148663433

Gene position: 4019737-4018862 (Counterclockwise)

Preceding gene: 148663434

Following gene: 148663432

Centisome position: 90.94

GC content: 64.38

Gene sequence:

>876_bases
ATGACAGCTACCGAGGAATTGACGTTCGAATCCACCTCGCGCTTTGCGGAAGTGGACGTCGACGGGCCGCTGAAACTGCA
CTACCACGAGGCCGGCGTGGGCAACGACCAGACGGTGGTGCTACTGCACGGCGGTGGGCCCGGCGCGGCGAGCTGGACGA
ACTTCTCGCGTAATATCGCGGTGCTGGCGCGGCACTTTCATGTGCTGGCCGTCGACCAGCCCGGTTACGGCCATTCCGAC
AAGCGGGCCGAGCACGGCCAGTTCAATCGCTATGCCGCGATGGCGCTGAAGGGGCTCTTCGATCAGCTGGGGCTGGGGCG
GGTACCGCTGGTGGGCAACTCGTTGGGCGGGGGAACCGCGGTCCGGTTTGCGCTGGACTACCCGGCCCGGGCAGGACGGT
TAGTGCTGATGGGCCCGGGGGGCCTGAGTATCAACCTGTTTGCGCCCGACCCGACCGAGGGAGTCAAACGGCTGTCGAAG
TTCTCCGTTGCGCCCACCCGGGAGAACCTCGAGGCGTTCCTGCGGGTCATGGTCTACGACAAGAACCTGATCACCCCCGA
GTTGGTGGATCAGCGGTTTGCGCTGGCCAGCACCCCGGAGTCGTTGACGGCAACACGGGCGATGGGAAAGTCGTTCGCCG
GAGCCGACTTCGAGGCCGGCATGATGTGGCGCGAGGTGTATCGGCTGCGCCAGCCGGTGTTGCTGATCTGGGGTCGTGAG
GACCGGGTCAACCCGCTGGACGGCGCGCTGGTTGCGTTGAAAACGATTCCGCGTGCGCAGCTGCACGTATTCGGGCAGTG
TGGGCATTGGGTGCAGGTGGAGAAGTTCGACGAGTTCAACAAGCTGACGATTGAATTTCTGGGAGGTGGCAGATGA

Upstream 100 bases:

>100_bases
TGCACGCCGCCAACGACCCCGAGCGGGCATACGTGATCTTTGGAAACCACGAGTTCGGGTTGCCGCCCGGCGACACCATG
GTCTAGCTGGTCTAGGCGAA

Downstream 100 bases:

>100_bases
GCATCCGGTCGCTGGGCTATCTGCGCATCGAGGCCACTGACATGGCTGCCTGGCGGGAGTATGGACTGAAGGTCCTGGGC
ATGGTCGAGGGCAAAGGGGC

Product: 2-hydroxy-6-phenylhexa-2,4-dienoic acid hydrolase

Products: NA

Alternate protein names: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; Meta-cleavage product hydrolase; MCP hydrolase

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTATEELTFESTSRFAEVDVDGPLKLHYHEAGVGNDQTVVLLHGGGPGAASWTNFSRNIAVLARHFHVLAVDQPGYGHSD
KRAEHGQFNRYAAMALKGLFDQLGLGRVPLVGNSLGGGTAVRFALDYPARAGRLVLMGPGGLSINLFAPDPTEGVKRLSK
FSVAPTRENLEAFLRVMVYDKNLITPELVDQRFALASTPESLTATRAMGKSFAGADFEAGMMWREVYRLRQPVLLIWGRE
DRVNPLDGALVALKTIPRAQLHVFGQCGHWVQVEKFDEFNKLTIEFLGGGR

Sequences:

>Translated_291_residues
MTATEELTFESTSRFAEVDVDGPLKLHYHEAGVGNDQTVVLLHGGGPGAASWTNFSRNIAVLARHFHVLAVDQPGYGHSD
KRAEHGQFNRYAAMALKGLFDQLGLGRVPLVGNSLGGGTAVRFALDYPARAGRLVLMGPGGLSINLFAPDPTEGVKRLSK
FSVAPTRENLEAFLRVMVYDKNLITPELVDQRFALASTPESLTATRAMGKSFAGADFEAGMMWREVYRLRQPVLLIWGRE
DRVNPLDGALVALKTIPRAQLHVFGQCGHWVQVEKFDEFNKLTIEFLGGGR
>Mature_290_residues
TATEELTFESTSRFAEVDVDGPLKLHYHEAGVGNDQTVVLLHGGGPGAASWTNFSRNIAVLARHFHVLAVDQPGYGHSDK
RAEHGQFNRYAAMALKGLFDQLGLGRVPLVGNSLGGGTAVRFALDYPARAGRLVLMGPGGLSINLFAPDPTEGVKRLSKF
SVAPTRENLEAFLRVMVYDKNLITPELVDQRFALASTPESLTATRAMGKSFAGADFEAGMMWREVYRLRQPVLLIWGRED
RVNPLDGALVALKTIPRAQLHVFGQCGHWVQVEKFDEFNKLTIEFLGGGR

Specific function: Catalyzes the hydrolysis of a carbon-carbon bond in 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA) to yield 9,17-dioxo-1,2,3,4,10,19- hexanorandrostan-5-oic acid (DOHNAA) and 2-hydroxy-hexa-2,4- dienoic acid (HHD). Is

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. HsaD family

Homologues:

Organism=Homo sapiens, GI189027141, Length=276, Percent_Identity=25.7246376811594, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87081721, Length=294, Percent_Identity=39.7959183673469, Blast_Score=203, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSAD_MYCTU (P96851)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842583
- PIR:   G70605
- RefSeq:   NP_218086.1
- RefSeq:   NP_338219.1
- PDB:   2VF2
- PDB:   2WUD
- PDB:   2WUE
- PDB:   2WUF
- PDB:   2WUG
- PDBsum:   2VF2
- PDBsum:   2WUD
- PDBsum:   2WUE
- PDBsum:   2WUF
- PDBsum:   2WUG
- ProteinModelPortal:   P96851
- SMR:   P96851
- EnsemblBacteria:   EBMYCT00000002034
- EnsemblBacteria:   EBMYCT00000070709
- GeneID:   887378
- GeneID:   922817
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3674
- KEGG:   mtu:Rv3569c
- TIGR:   MT3674
- TubercuList:   Rv3569c
- GeneTree:   EBGT00050000015213
- HOGENOM:   HBG748003
- OMA:   TEDVVEY
- ProtClustDB:   CLSK872205
- InterPro:   IPR000073
- PRINTS:   PR00111

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.7.1.8

Molecular weight: Translated: 31876; Mature: 31745

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: ACT_SITE 269-269 BINDING 54-54 BINDING 113-113 BINDING 115-115 BINDING 192-192 BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATEELTFESTSRFAEVDVDGPLKLHYHEAGVGNDQTVVLLHGGGPGAASWTNFSRNIA
CCCCCCCEECCCCCEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHH
VLARHFHVLAVDQPGYGHSDKRAEHGQFNRYAAMALKGLFDQLGLGRVPLVGNSLGGGTA
EEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCEE
VRFALDYPARAGRLVLMGPGGLSINLFAPDPTEGVKRLSKFSVAPTRENLEAFLRVMVYD
EEEEEECCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
KNLITPELVDQRFALASTPESLTATRAMGKSFAGADFEAGMMWREVYRLRQPVLLIWGRE
CCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCC
DRVNPLDGALVALKTIPRAQLHVFGQCGHWVQVEKFDEFNKLTIEFLGGGR
CCCCCCCHHEEEEHHCCCCCEEEEECCCCEEEEECCCCCCEEEEEEECCCC
>Mature Secondary Structure 
TATEELTFESTSRFAEVDVDGPLKLHYHEAGVGNDQTVVLLHGGGPGAASWTNFSRNIA
CCCCCCEECCCCCEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHH
VLARHFHVLAVDQPGYGHSDKRAEHGQFNRYAAMALKGLFDQLGLGRVPLVGNSLGGGTA
EEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCEE
VRFALDYPARAGRLVLMGPGGLSINLFAPDPTEGVKRLSKFSVAPTRENLEAFLRVMVYD
EEEEEECCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
KNLITPELVDQRFALASTPESLTATRAMGKSFAGADFEAGMMWREVYRLRQPVLLIWGRE
CCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCC
DRVNPLDGALVALKTIPRAQLHVFGQCGHWVQVEKFDEFNKLTIEFLGGGR
CCCCCCCHHEEEEHHCCCCCEEEEECCCCEEEEECCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036