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The map label for this gene is hsaB

Identifier: 148663431

GI number: 148663431

Start: 4017385

End: 4017948

Strand: Reverse

Name: hsaB

Synonym: MRA_3606

Alternate gene names: 148663431

Gene position: 4017948-4017385 (Counterclockwise)

Preceding gene: 148663432

Following gene: 148663430

Centisome position: 90.9

GC content: 63.65

Gene sequence:

>564_bases
ATGTCGGCTCAGATCGATCCACGCACGTTCCGCTCCGTGCTCGGTCAGTTCTGTACGGGGATCACTGTCATCACCACTGT
GCACGACGACGTGCCCGTCGGGTTTGCGTGCCAGTCGTTCGCGGCGCTGTCGTTGGAACCGCCGTTGGTGCTGTTCTGCC
CCACTAAGGTGTCGCGGTCCTGGCAGGCCATCGAGGCCAGCGGCCGGTTCTGCGTCAACGTACTGACCGAAAAGCAGAAA
GACGTCTCGGCGCGATTCGGCTCGAAGGAGCCGGACAAGTTCGCCGGTATCGATTGGCGCCCTTCGGAACTAGGCTCACC
GATCATCGAGGGATCGCTGGCCTACATTGACTGCACGGTGGCGTCGGTGCACGACGGCGGCGATCACTTCGTGGTGTTCG
GTGCCGTCGAGTCACTGTCAGAGGTCCCGGCGGTCAAGCCGCGGCCGCTGCTGTTCTATCGCGGCGACTACACCGGCATC
GAGCCGGAAAAGACCACGCCGGCGCACTGGCGCGACGACCTGGAGGCTTTCCTCATCACCACCACCCAGGACACCTGGCT
CTAG

Upstream 100 bases:

>100_bases
AAGTCGACGATCGGGACTGGATCGCCCGGGAGAGTACTGCGGTCAGCCTATGGGGTCACGATTTCACCGTAGGCGCTCGC
GGCTAGCTCGAAAGCAAACG

Downstream 100 bases:

>100_bases
TGGCGCGAGCAGACGCAAAATCGCCCATTTTGCGGGCGTGTTGGGCGATTTTGCGTCTGCTCGCCGTCGCCGGTAAGCAG
TCTGGTGAGCCACGTCCGGT

Product: putative oxidoreductase

Products: NAD(+); reduced flavin [C]

Alternate protein names: Flavin:NADH reductase

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MSAQIDPRTFRSVLGQFCTGITVITTVHDDVPVGFACQSFAALSLEPPLVLFCPTKVSRSWQAIEASGRFCVNVLTEKQK
DVSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTVASVHDGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDYTGI
EPEKTTPAHWRDDLEAFLITTTQDTWL

Sequences:

>Translated_187_residues
MSAQIDPRTFRSVLGQFCTGITVITTVHDDVPVGFACQSFAALSLEPPLVLFCPTKVSRSWQAIEASGRFCVNVLTEKQK
DVSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTVASVHDGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDYTGI
EPEKTTPAHWRDDLEAFLITTTQDTWL
>Mature_186_residues
SAQIDPRTFRSVLGQFCTGITVITTVHDDVPVGFACQSFAALSLEPPLVLFCPTKVSRSWQAIEASGRFCVNVLTEKQKD
VSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTVASVHDGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDYTGIE
PEKTTPAHWRDDLEAFLITTTQDTWL

Specific function: Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family

Homologues:

Organism=Escherichia coli, GI87081819, Length=158, Percent_Identity=27.2151898734177, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSAB_MYCTU (P96849)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842583
- PIR:   E70605
- RefSeq:   NP_218084.1
- RefSeq:   NP_338217.1
- ProteinModelPortal:   P96849
- SMR:   P96849
- EnsemblBacteria:   EBMYCT00000000930
- EnsemblBacteria:   EBMYCT00000069889
- GeneID:   887525
- GeneID:   922820
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3672
- KEGG:   mtu:Rv3567c
- TIGR:   MT3672
- TubercuList:   Rv3567c
- GeneTree:   EBGT00050000016052
- HOGENOM:   HBG730363
- OMA:   RSWAAIE
- ProtClustDB:   CLSK792566
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- Gene3D:   G3DSA:2.30.110.10
- SMART:   SM00903

Pfam domain/function: PF01613 Flavin_Reduct; SSF50475 FMN_binding

EC number: 1.6.8.- [C]

Molecular weight: Translated: 20539; Mature: 20408

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQIDPRTFRSVLGQFCTGITVITTVHDDVPVGFACQSFAALSLEPPLVLFCPTKVSRS
CCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHEEEECCCCEEEECCCCCCCC
WQAIEASGRFCVNVLTEKQKDVSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTV
EEEECCCCCEEEEEHHHCHHHHHHHCCCCCCCHHCCCCCCHHHHCCHHHCCCEEEEEEEE
ASVHDGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDYTGIEPEKTTPAHWRDDLEAFLIT
EEEECCCCEEEEEEEHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHCCCEEEEEE
TTQDTWL
ECCCCCC
>Mature Secondary Structure 
SAQIDPRTFRSVLGQFCTGITVITTVHDDVPVGFACQSFAALSLEPPLVLFCPTKVSRS
CCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHEEEECCCCEEEECCCCCCCC
WQAIEASGRFCVNVLTEKQKDVSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTV
EEEECCCCCEEEEEHHHCHHHHHHHCCCCCCCHHCCCCCCHHHHCCHHHCCCEEEEEEEE
ASVHDGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDYTGIEPEKTTPAHWRDDLEAFLIT
EEEECCCCEEEEEEEHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHCCCEEEEEE
TTQDTWL
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036