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The map label for this gene is gph2 [H]

Identifier: 148653915

GI number: 148653915

Start: 2641593

End: 2642300

Strand: Direct

Name: gph2 [H]

Synonym: PsycPRwf_2118

Alternate gene names: 148653915

Gene position: 2641593-2642300 (Clockwise)

Preceding gene: 148653914

Following gene: 148653916

Centisome position: 88.67

GC content: 47.88

Gene sequence:

>708_bases
ATGTCAACAAATTATGTCAAAGCCGTCTTATTTGATTTAGATGGTACTCTCATTGATACCGCCGCTGATTTTATCCGTAT
TATTAAGCGTATGTCAGAGCAAAATGGCTGGCAAGTACCGCCAAGTCAAGCCATTCGTGAGCAAGTGTCGGCCGGTGCAG
GTGCTATGGTGAGCCTGATGCTCAAGCACAATGAGCAGCCGCTTACTGAAGAGCAGATTCAGCACTACCGCCAGCAATTT
TTGGATGAGTATGAGGCCGATATCTGTGTTGACAGCCAGGTGTTTACAGAGCTTGAGCCACTGCTAAGCTTTTATGAAAA
AAGCGGCATTCCTTGGGGTATTGTTACCAATAAGCCGCGCTATTTGGCCACCAAGTTATTACAAAGTCTTGCTCTCGATG
AGCGCTGCTCAGTGCTGGTTTGCCCCGATGATGTGAGCAATACCAAGCCCGACCCTGAGCCTATGTATTTGGCATTAGAG
AAGTTAGAATTGCCACGCGGTATTGCCAACAGTGTGATTTATATCGGGGATCATATCCGTGATGTGCAAGCAGGTAGTGC
AGCAGGCATGACGACGGTTCTGGCCAGCTATGGCTATATCCCGCCAGAGGACCAAGATGACTTGGAAGCTTGGGGCGCCG
ATTATATTGCCGACACGCCCAAGGCCTTGGTAAAACTACTGCGCTCAAAGCAGTTTGATTATTTATAA

Upstream 100 bases:

>100_bases
CGTCGTAAAACCCGCTTAATTGCCCATTTAGCGCTCTATTTAATGGGCCACTGAGCTTAAGTTTAGCGTTATAAACACAC
CTTTTAAAAAGTACAACATT

Downstream 100 bases:

>100_bases
CTTAGTACGTATAACTTAGTATTTATCACTTAACAGTTATCACTTAGTATTTACCATTTAATAAAACCTCTACCAAAATA
GTAGTGACCTTATGACTCAA

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSTNYVKAVLFDLDGTLIDTAADFIRIIKRMSEQNGWQVPPSQAIREQVSAGAGAMVSLMLKHNEQPLTEEQIQHYRQQF
LDEYEADICVDSQVFTELEPLLSFYEKSGIPWGIVTNKPRYLATKLLQSLALDERCSVLVCPDDVSNTKPDPEPMYLALE
KLELPRGIANSVIYIGDHIRDVQAGSAAGMTTVLASYGYIPPEDQDDLEAWGADYIADTPKALVKLLRSKQFDYL

Sequences:

>Translated_235_residues
MSTNYVKAVLFDLDGTLIDTAADFIRIIKRMSEQNGWQVPPSQAIREQVSAGAGAMVSLMLKHNEQPLTEEQIQHYRQQF
LDEYEADICVDSQVFTELEPLLSFYEKSGIPWGIVTNKPRYLATKLLQSLALDERCSVLVCPDDVSNTKPDPEPMYLALE
KLELPRGIANSVIYIGDHIRDVQAGSAAGMTTVLASYGYIPPEDQDDLEAWGADYIADTPKALVKLLRSKQFDYL
>Mature_234_residues
STNYVKAVLFDLDGTLIDTAADFIRIIKRMSEQNGWQVPPSQAIREQVSAGAGAMVSLMLKHNEQPLTEEQIQHYRQQFL
DEYEADICVDSQVFTELEPLLSFYEKSGIPWGIVTNKPRYLATKLLQSLALDERCSVLVCPDDVSNTKPDPEPMYLALEK
LELPRGIANSVIYIGDHIRDVQAGSAAGMTTVLASYGYIPPEDQDDLEAWGADYIADTPKALVKLLRSKQFDYL

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=213, Percent_Identity=29.5774647887324, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 26320; Mature: 26189

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNYVKAVLFDLDGTLIDTAADFIRIIKRMSEQNGWQVPPSQAIREQVSAGAGAMVSLM
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHH
LKHNEQPLTEEQIQHYRQQFLDEYEADICVDSQVFTELEPLLSFYEKSGIPWGIVTNKPR
HHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCH
YLATKLLQSLALDERCSVLVCPDDVSNTKPDPEPMYLALEKLELPRGIANSVIYIGDHIR
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCEEEECCHHH
DVQAGSAAGMTTVLASYGYIPPEDQDDLEAWGADYIADTPKALVKLLRSKQFDYL
HHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STNYVKAVLFDLDGTLIDTAADFIRIIKRMSEQNGWQVPPSQAIREQVSAGAGAMVSLM
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHH
LKHNEQPLTEEQIQHYRQQFLDEYEADICVDSQVFTELEPLLSFYEKSGIPWGIVTNKPR
HHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCH
YLATKLLQSLALDERCSVLVCPDDVSNTKPDPEPMYLALEKLELPRGIANSVIYIGDHIR
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCEEEECCHHH
DVQAGSAAGMTTVLASYGYIPPEDQDDLEAWGADYIADTPKALVKLLRSKQFDYL
HHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]