| Definition | Psychrobacter sp. PRwf-1 chromosome, complete genome. |
|---|---|
| Accession | NC_009524 |
| Length | 2,978,976 |
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The map label for this gene is nadE [H]
Identifier: 148653679
GI number: 148653679
Start: 2339811
End: 2341469
Strand: Direct
Name: nadE [H]
Synonym: PsycPRwf_1882
Alternate gene names: 148653679
Gene position: 2339811-2341469 (Clockwise)
Preceding gene: 148653677
Following gene: 148653681
Centisome position: 78.54
GC content: 47.2
Gene sequence:
>1659_bases ATGTCTAATGATAACAGTAGCACGGCAGCAAAAGGAAGTTCGCTGAAATTTGCAATGGCACAGTCCCACTTTATGGTCGG TGACATTCAAACCAATATCGATAAAATGCGTAGTTTGGCTATTGAAGCCCGCGACAATGGTGCTGACGTCATCGTCTTCC CAGAGCTGGCTTTGCTTGGCTATCCACCAGAGGACTTGCTGCTTCGCCCAAGTTTATCAGACCGCGTAAAAGCTGCCCTT AGCTCACTGAGCGATATTAATGATATCGTGATGATTATCGGCTATCCGCATGTCGATTATCATGGCACCTTTAACTCAGC TGCCATTATCCAAAACGGCCAACAAAAGGGCTTTTATCACAAGCAGTATCTGCCAAACTATGGCGTCTTTGATGAGCGCC GCTACTTTGACAAAGGCCGCAATCAAGTATTATTCGACTACCAAGGCGTGACCATTGGCCTGCTGATCTGTGAAGACTTA TGGCAAGAAGAGCCCATCAAGGCTCTAAAAGACCAAGGTGCCGACTTAGTGGTTACCATCAATGCCTCGCCATTTGAGGC CGGTAAGCAGCACACCCGTCAAGCCCTATTGAATAAGCGTGCCACAGATAATCAGTTACCAATCATCTATGTCAATGCAG TGGGCGGTCAAGATGACCTGGTTTTTGATGGCGGCTCGATGGCCATTCAGGCCAATGGTGACGTAGCGCATGAAGCCCCT AGATTCTTAGAGCACATGCTGTATGCTACTTATGATATAAAAAATCACACTTTTGATAGCCAAACCAAAGCACCTTTACA GCTCAGCTCAGAGTCTGAGACGTATCAAGCCTTAGTGGTGGGTCTACGTGACTATGTCAATCACTCAGGCTTTGAAGGCG TGATTGTGGGCCTATCAGGTGGCATTGATTCGGCACTGACCTTATGCGTGGCCGTGGATGCGCTAGGCTCTGATAAAGTA TATGCGGTTATGATGCCCTATGAGTACACCTCTCAAATCAGCTTAGAGGATGCCCAGGCTCAAGCGCGCCGACTCAATGT TTCCTATACGGTATGCCCCATCCATGATGCGGTAGATGGTATGCGTCATACGTTAGCCCCATTATTTAGCAAGGCCAAAG CAGATACCACGGAGGAGAACATCCAAGCCCGTGCACGCGGCATGGTACTGATGGCGCTATCAAATAAATTTGGCCATTTA GTCATCACCACAGGCAATAAATCAGAAATGGCAGTGGGTTATGCCACCTTGTATGGGGATATGGCCGGCGGTTTTGATGT CTTAAAAGATGTGTATAAAACTCAAGTATACGCTCTGGCCAATTACCGCAATCGCCTAGAAGATACCAATGTTATTCCTG AGCGTGTTATTACCCGTCCTCCTTCTGCTGAGCTACGTCCAGATCAAGTGGACCAAGACAGCCTACCCGATTACGACCTA TTAGATGCCATTTTAAAAGACTATATCGACAATGACTTAGGCTTTAATGAGATTACTGCTAAAGGCTTTGATCCTGAGGT AGTGCGTCATGTTATCAAGTTGGTAGATAGAAGCGAATATAAGCGCCGGCAAGCAGCCATTGGCACTAAGATTAGCCACA AAGCGTTTGGTCGTGAGCGCCGCTATCCCTTAGTTAATGGCTGGAGCATTGAAGGCTAA
Upstream 100 bases:
>100_bases GCTAATAGCTTGCCTAAAAATGTGACACCAACAAAACTCAATAAGTTGCATTTGACCGGTGATTATTAGCAATAAGACCC ATTACGAATAGGACCCAATA
Downstream 100 bases:
>100_bases CGGGCCCAAAACTGTTCAGTAAATGACCTCTATTACTATAATAAAAGAGCCCTGGTGGCTCTTTTATTATATGCATAACA TGCCAATTTTGCGTCTGTCT
Product: NAD+ synthetase
Products: NA
Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]
Number of amino acids: Translated: 552; Mature: 551
Protein sequence:
>552_residues MSNDNSSTAAKGSSLKFAMAQSHFMVGDIQTNIDKMRSLAIEARDNGADVIVFPELALLGYPPEDLLLRPSLSDRVKAAL SSLSDINDIVMIIGYPHVDYHGTFNSAAIIQNGQQKGFYHKQYLPNYGVFDERRYFDKGRNQVLFDYQGVTIGLLICEDL WQEEPIKALKDQGADLVVTINASPFEAGKQHTRQALLNKRATDNQLPIIYVNAVGGQDDLVFDGGSMAIQANGDVAHEAP RFLEHMLYATYDIKNHTFDSQTKAPLQLSSESETYQALVVGLRDYVNHSGFEGVIVGLSGGIDSALTLCVAVDALGSDKV YAVMMPYEYTSQISLEDAQAQARRLNVSYTVCPIHDAVDGMRHTLAPLFSKAKADTTEENIQARARGMVLMALSNKFGHL VITTGNKSEMAVGYATLYGDMAGGFDVLKDVYKTQVYALANYRNRLEDTNVIPERVITRPPSAELRPDQVDQDSLPDYDL LDAILKDYIDNDLGFNEITAKGFDPEVVRHVIKLVDRSEYKRRQAAIGTKISHKAFGRERRYPLVNGWSIEG
Sequences:
>Translated_552_residues MSNDNSSTAAKGSSLKFAMAQSHFMVGDIQTNIDKMRSLAIEARDNGADVIVFPELALLGYPPEDLLLRPSLSDRVKAAL SSLSDINDIVMIIGYPHVDYHGTFNSAAIIQNGQQKGFYHKQYLPNYGVFDERRYFDKGRNQVLFDYQGVTIGLLICEDL WQEEPIKALKDQGADLVVTINASPFEAGKQHTRQALLNKRATDNQLPIIYVNAVGGQDDLVFDGGSMAIQANGDVAHEAP RFLEHMLYATYDIKNHTFDSQTKAPLQLSSESETYQALVVGLRDYVNHSGFEGVIVGLSGGIDSALTLCVAVDALGSDKV YAVMMPYEYTSQISLEDAQAQARRLNVSYTVCPIHDAVDGMRHTLAPLFSKAKADTTEENIQARARGMVLMALSNKFGHL VITTGNKSEMAVGYATLYGDMAGGFDVLKDVYKTQVYALANYRNRLEDTNVIPERVITRPPSAELRPDQVDQDSLPDYDL LDAILKDYIDNDLGFNEITAKGFDPEVVRHVIKLVDRSEYKRRQAAIGTKISHKAFGRERRYPLVNGWSIEG >Mature_551_residues SNDNSSTAAKGSSLKFAMAQSHFMVGDIQTNIDKMRSLAIEARDNGADVIVFPELALLGYPPEDLLLRPSLSDRVKAALS SLSDINDIVMIIGYPHVDYHGTFNSAAIIQNGQQKGFYHKQYLPNYGVFDERRYFDKGRNQVLFDYQGVTIGLLICEDLW QEEPIKALKDQGADLVVTINASPFEAGKQHTRQALLNKRATDNQLPIIYVNAVGGQDDLVFDGGSMAIQANGDVAHEAPR FLEHMLYATYDIKNHTFDSQTKAPLQLSSESETYQALVVGLRDYVNHSGFEGVIVGLSGGIDSALTLCVAVDALGSDKVY AVMMPYEYTSQISLEDAQAQARRLNVSYTVCPIHDAVDGMRHTLAPLFSKAKADTTEENIQARARGMVLMALSNKFGHLV ITTGNKSEMAVGYATLYGDMAGGFDVLKDVYKTQVYALANYRNRLEDTNVIPERVITRPPSAELRPDQVDQDSLPDYDLL DAILKDYIDNDLGFNEITAKGFDPEVVRHVIKLVDRSEYKRRQAAIGTKISHKAFGRERRYPLVNGWSIEG
Specific function: Can use both glutamine or ammonia as a nitrogen source [H]
COG id: COG0171
COG function: function code H; NAD synthase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 CN hydrolase domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014445 - InterPro: IPR022310 - InterPro: IPR003694 - InterPro: IPR000132 - InterPro: IPR003010 - InterPro: IPR014729 [H]
Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]
EC number: =6.3.5.1 [H]
Molecular weight: Translated: 61156; Mature: 61025
Theoretical pI: Translated: 5.15; Mature: 5.15
Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSNDNSSTAAKGSSLKFAMAQSHFMVGDIQTNIDKMRSLAIEARDNGADVIVFPELALLG CCCCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHEEEEECCCCCCEEEECCCEEEC YPPEDLLLRPSLSDRVKAALSSLSDINDIVMIIGYPHVDYHGTFNSAAIIQNGQQKGFYH CCCHHHEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCH KQYLPNYGVFDERRYFDKGRNQVLFDYQGVTIGLLICEDLWQEEPIKALKDQGADLVVTI HHCCCCCCCCCCHHHHHCCCCEEEEEECCCEEEEHHHHHHCCCCCHHHHHCCCCEEEEEE NASPFEAGKQHTRQALLNKRATDNQLPIIYVNAVGGQDDLVFDGGSMAIQANGDVAHEAP ECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCEEEEECCCCHHHHH RFLEHMLYATYDIKNHTFDSQTKAPLQLSSESETYQALVVGLRDYVNHSGFEGVIVGLSG HHHHHHHHHEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECC GIDSALTLCVAVDALGSDKVYAVMMPYEYTSQISLEDAQAQARRLNVSYTVCPIHDAVDG CHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEECHHHHHHHHHCCEEEEEECCHHHHHH MRHTLAPLFSKAKADTTEENIQARARGMVLMALSNKFGHLVITTGNKSEMAVGYATLYGD HHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCEEEEHHHHHCC MAGGFDVLKDVYKTQVYALANYRNRLEDTNVIPERVITRPPSAELRPDQVDQDSLPDYDL CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHH LDAILKDYIDNDLGFNEITAKGFDPEVVRHVIKLVDRSEYKRRQAAIGTKISHKAFGRER HHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC RYPLVNGWSIEG CCCCCCCCCCCC >Mature Secondary Structure SNDNSSTAAKGSSLKFAMAQSHFMVGDIQTNIDKMRSLAIEARDNGADVIVFPELALLG CCCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHEEEEECCCCCCEEEECCCEEEC YPPEDLLLRPSLSDRVKAALSSLSDINDIVMIIGYPHVDYHGTFNSAAIIQNGQQKGFYH CCCHHHEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCH KQYLPNYGVFDERRYFDKGRNQVLFDYQGVTIGLLICEDLWQEEPIKALKDQGADLVVTI HHCCCCCCCCCCHHHHHCCCCEEEEEECCCEEEEHHHHHHCCCCCHHHHHCCCCEEEEEE NASPFEAGKQHTRQALLNKRATDNQLPIIYVNAVGGQDDLVFDGGSMAIQANGDVAHEAP ECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCEEEEECCCCHHHHH RFLEHMLYATYDIKNHTFDSQTKAPLQLSSESETYQALVVGLRDYVNHSGFEGVIVGLSG HHHHHHHHHEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECC GIDSALTLCVAVDALGSDKVYAVMMPYEYTSQISLEDAQAQARRLNVSYTVCPIHDAVDG CHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEECHHHHHHHHHCCEEEEEECCHHHHHH MRHTLAPLFSKAKADTTEENIQARARGMVLMALSNKFGHLVITTGNKSEMAVGYATLYGD HHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCEEEEHHHHHCC MAGGFDVLKDVYKTQVYALANYRNRLEDTNVIPERVITRPPSAELRPDQVDQDSLPDYDL CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHH LDAILKDYIDNDLGFNEITAKGFDPEVVRHVIKLVDRSEYKRRQAAIGTKISHKAFGRER HHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC RYPLVNGWSIEG CCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10910347 [H]