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The map label for this gene is pilT [H]

Identifier: 148653681

GI number: 148653681

Start: 2342450

End: 2343529

Strand: Direct

Name: pilT [H]

Synonym: PsycPRwf_1884

Alternate gene names: 148653681

Gene position: 2342450-2343529 (Clockwise)

Preceding gene: 148653679

Following gene: 148653683

Centisome position: 78.63

GC content: 49.26

Gene sequence:

>1080_bases
ATGATTCAAGGGATAGATGAGGTGACACACGCTCACGCTTTAGATTCACTCACTGCGCTACTACGCCATACTCAAAGCTT
AAAGGCTTCAGATCTGCACCTGTCAGCAGGAATGCCCGCCATGCTGCGAATCGATGGGGAGATAACCTCGACCAACCTAC
CCGCCTTAACGCATACCCAAATTCAGCAGTATATTGCCAGCACCTTAAGCCCTGCGCAAATGAGTGAATTTGAACAGCAC
ATGACATTGGACTATGCGTTGGATGTGGCCGATGTGGGGCGATTTCGGGTCAATATATTTCAGCAGCACCGAGGTATTGC
AGCTGTATTTCGTGCCATCCCCGATGCCCTATTGTCTTTGCAGCAGCTCGACTTATCCAGTGATAATAAGGCCACCTTGC
AACAAATAACCCAGCTCAATCATGGGCTGGTATTGGTCACCGGCCCCACCGGCTGCGGAAAATCAACCACAGTGGCCGCT
TTGGTTGATCATATTAACCAAACACGCGCCGCCCACATTCTGACCATCGAAGACCCCATTGAATTTATTTATCACGCCAA
AAAAAGCTTAATCAATCAGCGGGAAGTGGCGCGTCATACCGCAGATTATACGAAGGCCCTACGCTCAGCTTTGCGTGAAG
ATCCCGACGTTATTGTGATTGGTGAGCTGCGTGATTTGGCCAGTATACGTTTGGCGCTTACTGCAGCTGAGACCGGGCAT
TTGGTACTGGCAACGCTACATACCCGCTCTGCGATTAAAAGCATCGACAGAATTGTGGATGTATTTGCAGCCCATGAAAA
GGACATGATCCGATTTTTGTTGTCCGAATCGCTACAAGCTGTGATCTCGCAAATATTAATTAAGCGCCCTGAGGGAGGAC
GAATAGCCGCTCACGAGATTATGATGGCCACCCCTGCCATCTGTAACATGATTCGCCAAAACAAACTGGCACAAATGTAT
TCCGCCATTCAAACTGGCCACAGCCAAGGCATGCAGACTCTAGACCAAGCGCTCACCGATCTAATCCAGCGCAAACTTAT
TAGCCCACATAGCGCTCAGCATCATGCCAAATTATGCTAG

Upstream 100 bases:

>100_bases
TATAAGTGGTGCGCTCTATTATAAGTGGTGCGCTCTATTTTATAAATGCAAACAAATACTTGCTCCTAAGCACTCCAAGC
TTGTTATAGTGGGTGTCCAC

Downstream 100 bases:

>100_bases
GTGATAAAAAGCGTGAAAGCGCATCTTACCTCATTTACCATAAAAAAAGCCTGTTTAAAAACAGGCTTTTAGCAATAGGC
TTTTAAAAAGGCCAACTCAG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MIQGIDEVTHAHALDSLTALLRHTQSLKASDLHLSAGMPAMLRIDGEITSTNLPALTHTQIQQYIASTLSPAQMSEFEQH
MTLDYALDVADVGRFRVNIFQQHRGIAAVFRAIPDALLSLQQLDLSSDNKATLQQITQLNHGLVLVTGPTGCGKSTTVAA
LVDHINQTRAAHILTIEDPIEFIYHAKKSLINQREVARHTADYTKALRSALREDPDVIVIGELRDLASIRLALTAAETGH
LVLATLHTRSAIKSIDRIVDVFAAHEKDMIRFLLSESLQAVISQILIKRPEGGRIAAHEIMMATPAICNMIRQNKLAQMY
SAIQTGHSQGMQTLDQALTDLIQRKLISPHSAQHHAKLC

Sequences:

>Translated_359_residues
MIQGIDEVTHAHALDSLTALLRHTQSLKASDLHLSAGMPAMLRIDGEITSTNLPALTHTQIQQYIASTLSPAQMSEFEQH
MTLDYALDVADVGRFRVNIFQQHRGIAAVFRAIPDALLSLQQLDLSSDNKATLQQITQLNHGLVLVTGPTGCGKSTTVAA
LVDHINQTRAAHILTIEDPIEFIYHAKKSLINQREVARHTADYTKALRSALREDPDVIVIGELRDLASIRLALTAAETGH
LVLATLHTRSAIKSIDRIVDVFAAHEKDMIRFLLSESLQAVISQILIKRPEGGRIAAHEIMMATPAICNMIRQNKLAQMY
SAIQTGHSQGMQTLDQALTDLIQRKLISPHSAQHHAKLC
>Mature_359_residues
MIQGIDEVTHAHALDSLTALLRHTQSLKASDLHLSAGMPAMLRIDGEITSTNLPALTHTQIQQYIASTLSPAQMSEFEQH
MTLDYALDVADVGRFRVNIFQQHRGIAAVFRAIPDALLSLQQLDLSSDNKATLQQITQLNHGLVLVTGPTGCGKSTTVAA
LVDHINQTRAAHILTIEDPIEFIYHAKKSLINQREVARHTADYTKALRSALREDPDVIVIGELRDLASIRLALTAAETGH
LVLATLHTRSAIKSIDRIVDVFAAHEKDMIRFLLSESLQAVISQILIKRPEGGRIAAHEIMMATPAICNMIRQNKLAQMY
SAIQTGHSQGMQTLDQALTDLIQRKLISPHSAQHHAKLC

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=308, Percent_Identity=44.4805194805195, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1789723, Length=366, Percent_Identity=31.4207650273224, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1786296, Length=294, Percent_Identity=32.312925170068, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39493; Mature: 39493

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQGIDEVTHAHALDSLTALLRHTQSLKASDLHLSAGMPAMLRIDGEITSTNLPALTHTQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCEEECCCCCCHHHHH
IQQYIASTLSPAQMSEFEQHMTLDYALDVADVGRFRVNIFQQHRGIAAVFRAIPDALLSL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
QQLDLSSDNKATLQQITQLNHGLVLVTGPTGCGKSTTVAALVDHINQTRAAHILTIEDPI
HHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHHH
EFIYHAKKSLINQREVARHTADYTKALRSALREDPDVIVIGELRDLASIRLALTAAETGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHEEECCCCC
LVLATLHTRSAIKSIDRIVDVFAAHEKDMIRFLLSESLQAVISQILIKRPEGGRIAAHEI
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
MMATPAICNMIRQNKLAQMYSAIQTGHSQGMQTLDQALTDLIQRKLISPHSAQHHAKLC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure
MIQGIDEVTHAHALDSLTALLRHTQSLKASDLHLSAGMPAMLRIDGEITSTNLPALTHTQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCEEECCCCCCHHHHH
IQQYIASTLSPAQMSEFEQHMTLDYALDVADVGRFRVNIFQQHRGIAAVFRAIPDALLSL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
QQLDLSSDNKATLQQITQLNHGLVLVTGPTGCGKSTTVAALVDHINQTRAAHILTIEDPI
HHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHHH
EFIYHAKKSLINQREVARHTADYTKALRSALREDPDVIVIGELRDLASIRLALTAAETGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHEEECCCCC
LVLATLHTRSAIKSIDRIVDVFAAHEKDMIRFLLSESLQAVISQILIKRPEGGRIAAHEI
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
MMATPAICNMIRQNKLAQMYSAIQTGHSQGMQTLDQALTDLIQRKLISPHSAQHHAKLC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]