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The map label for this gene is gshB [H]

Identifier: 148652334

GI number: 148652334

Start: 624098

End: 625075

Strand: Direct

Name: gshB [H]

Synonym: PsycPRwf_0522

Alternate gene names: 148652334

Gene position: 624098-625075 (Clockwise)

Preceding gene: 148652333

Following gene: 148652335

Centisome position: 20.95

GC content: 45.5

Gene sequence:

>978_bases
ATGAGTCAACATAGCGCCCAAAAACTACTTAATATTTTAGTGGTGATGGATCCAATCGAAACGGTGAACTATAAAAAAGA
CTCAACATTAGCCATGATGTGGTCTGCCAGTGATCGAGGACATGGTTTGGGGTATTGTCAAATTCATGACTTATGGCTGG
ATAAGGGCGAGTTAAAAGTAGATGCGCAAGCGGTCACGGTTCGCCGTGACCCTAACGATTTTTACAGTTTAGCGGATAAG
GTAACTCAGCCAATTACTGATTATGATGTCATCTTAATGCGTAAAGATCCGCCATTTAACATGCGCTTTTTATACAGTAC
TTATATGTTAGATCATGCCAAGGCAGCGGGGGTATTGGTGGTCAATGACCCTCAAGCGATTCGCGACTGTAACGAGAAGC
TTTTTGCCACCTGGTTTAGCGACTTAATGAGTCCCACCATAGTGACCAGCAAGCAGCAGCATATTCGCAAGTTTATCCAA
GAGCATCAAGATGTTATTGTAAAGCCTTTGGATGGTATGGGCGGTACCGGTATCTTTAGATTGACGCAAGACAGTCCCAA
TATTGGCGCCACGCTTGAGATGCTTACTGAACTTGAGACTTTACCGATTATGGCGCAGCGTTATTTGCCAGAAATTAAGC
AGGGCGATAAGCGAGTATTGATTGTTGATGGTGAAATTGTTCCCTTTAGTCTGGCGCGCATCCCCACAAAAGGCGAAACC
CGCGGCAACCTAGCCGCCGGTGGACAGGGTGTGGCTATGCCAGTGACTGAGCAGGAGATGGCTGTGGCCAAGAAGGTGGC
GCCTATTGTTAAGCAAAAAGGCTTGATGTTTGTTGGTCTAGATTTGATCGGGGCCAGAATTACAGAGATTAACGTCACCA
GCCCCACCTGTGTACGTGAAATTAACGACCAATGTGGTACCGATATCGCGACCGATTTAATTATCGCCATTGAGAAAAAA
TGCACTCAACATTCATAA

Upstream 100 bases:

>100_bases
TTAAGTCAGCTAAACTTGTAAAAATGAAGCCTAGCCATCACCCTAACTATAATCATCTTATCTTTGTCAGTTGCTGACAG
TCATAACGGAGTGAATTACC

Downstream 100 bases:

>100_bases
CTGGGATGGCCATAAGTAGAGGGAATTGCCATAATACGGCAATGTTTTGTTAATTAATCTTACAAAATAATTAAATAGTT
TATCGTTAGTAGGGTGGTTA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSQHSAQKLLNILVVMDPIETVNYKKDSTLAMMWSASDRGHGLGYCQIHDLWLDKGELKVDAQAVTVRRDPNDFYSLADK
VTQPITDYDVILMRKDPPFNMRFLYSTYMLDHAKAAGVLVVNDPQAIRDCNEKLFATWFSDLMSPTIVTSKQQHIRKFIQ
EHQDVIVKPLDGMGGTGIFRLTQDSPNIGATLEMLTELETLPIMAQRYLPEIKQGDKRVLIVDGEIVPFSLARIPTKGET
RGNLAAGGQGVAMPVTEQEMAVAKKVAPIVKQKGLMFVGLDLIGARITEINVTSPTCVREINDQCGTDIATDLIIAIEKK
CTQHS

Sequences:

>Translated_325_residues
MSQHSAQKLLNILVVMDPIETVNYKKDSTLAMMWSASDRGHGLGYCQIHDLWLDKGELKVDAQAVTVRRDPNDFYSLADK
VTQPITDYDVILMRKDPPFNMRFLYSTYMLDHAKAAGVLVVNDPQAIRDCNEKLFATWFSDLMSPTIVTSKQQHIRKFIQ
EHQDVIVKPLDGMGGTGIFRLTQDSPNIGATLEMLTELETLPIMAQRYLPEIKQGDKRVLIVDGEIVPFSLARIPTKGET
RGNLAAGGQGVAMPVTEQEMAVAKKVAPIVKQKGLMFVGLDLIGARITEINVTSPTCVREINDQCGTDIATDLIIAIEKK
CTQHS
>Mature_324_residues
SQHSAQKLLNILVVMDPIETVNYKKDSTLAMMWSASDRGHGLGYCQIHDLWLDKGELKVDAQAVTVRRDPNDFYSLADKV
TQPITDYDVILMRKDPPFNMRFLYSTYMLDHAKAAGVLVVNDPQAIRDCNEKLFATWFSDLMSPTIVTSKQQHIRKFIQE
HQDVIVKPLDGMGGTGIFRLTQDSPNIGATLEMLTELETLPIMAQRYLPEIKQGDKRVLIVDGEIVPFSLARIPTKGETR
GNLAAGGQGVAMPVTEQEMAVAKKVAPIVKQKGLMFVGLDLIGARITEINVTSPTCVREINDQCGTDIATDLIIAIEKKC
TQHS

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=316, Percent_Identity=50.9493670886076, Blast_Score=346, Evalue=1e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 36129; Mature: 35998

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHSAQKLLNILVVMDPIETVNYKKDSTLAMMWSASDRGHGLGYCQIHDLWLDKGELKV
CCCHHHHHHHHHHHEECCHHHCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEECCCCEEE
DAQAVTVRRDPNDFYSLADKVTQPITDYDVILMRKDPPFNMRFLYSTYMLDHAKAAGVLV
EEEEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHCCEEE
VNDPQAIRDCNEKLFATWFSDLMSPTIVTSKQQHIRKFIQEHQDVIVKPLDGMGGTGIFR
ECCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCEEEECCCCCCCCEEEE
LTQDSPNIGATLEMLTELETLPIMAQRYLPEIKQGDKRVLIVDGEIVPFSLARIPTKGET
EECCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEECCCCCEEHHEECCCCCCC
RGNLAAGGQGVAMPVTEQEMAVAKKVAPIVKQKGLMFVGLDLIGARITEINVTSPTCVRE
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEEEHHHHCCEEEEEECCCCHHHHH
INDQCGTDIATDLIIAIEKKCTQHS
HHHHHCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQHSAQKLLNILVVMDPIETVNYKKDSTLAMMWSASDRGHGLGYCQIHDLWLDKGELKV
CCHHHHHHHHHHHEECCHHHCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEECCCCEEE
DAQAVTVRRDPNDFYSLADKVTQPITDYDVILMRKDPPFNMRFLYSTYMLDHAKAAGVLV
EEEEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHCCEEE
VNDPQAIRDCNEKLFATWFSDLMSPTIVTSKQQHIRKFIQEHQDVIVKPLDGMGGTGIFR
ECCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCEEEECCCCCCCCEEEE
LTQDSPNIGATLEMLTELETLPIMAQRYLPEIKQGDKRVLIVDGEIVPFSLARIPTKGET
EECCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEECCCCCEEHHEECCCCCCC
RGNLAAGGQGVAMPVTEQEMAVAKKVAPIVKQKGLMFVGLDLIGARITEINVTSPTCVRE
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEEEHHHHCCEEEEEECCCCHHHHH
INDQCGTDIATDLIIAIEKKCTQHS
HHHHHCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]