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The map label for this gene is thyA [H]

Identifier: 148643794

GI number: 148643794

Start: 1782978

End: 1783676

Strand: Reverse

Name: thyA [H]

Synonym: Msm_1734

Alternate gene names: 148643794

Gene position: 1783676-1782978 (Counterclockwise)

Preceding gene: 148643796

Following gene: 148643792

Centisome position: 96.25

GC content: 33.48

Gene sequence:

>699_bases
ATGCTATCTATCAATCAATGTTATTTGGATTTTGTTAAAAAAATCTTAAACCAGGGAAAGGAAACATATAAAGACAGCAA
CCACCATTTAAAAGAAAGTTTAGGTAATTTTTACGTAATTGATGATCCGTTAAACTTGAAATTTGTGGCAAAATACCAGC
ATATGACTACTGATATGATGCTAAACGAAATCAAATCAGGAAAGTACGATATTGAAGGCTGTCCTATTAAAAGTGATGCA
CTCTATGAATATGTTAAGTCATTTGAAAACAGTGACGATCAGGGTTTTGTATACACTTATCCAAACCGAATATTGGCTCA
TTTTGATGTTGACCAGTTTGAAGTGATGAAAAAAAGAATGTTGCATGCAATTGGAAGTAACCGTGCAGTAGCTGTTACAT
ATGACCCTAAATTGGATCAGGACCGTGAAGACATTCCATGTTTACAGTTCCTGCAATGTATTGTCCGTAACAATGAATTA
ACAATCCACTGCTTATTCAGGTCCAACGACATCTTTGGAGCTTTCTATTCAAATATGTTTTTTATAACATATATCGGAAT
TAAAATGAAAGAAGAGCTTAATAAAGAGTTATTAGGTGAAAAAGTAAACTTCGGAGGAATCCATTACCACTCCACCAGCG
GCCATATCTACAGCAATGATATGAAAGCTGCTAAAAAATTAATAGCTATTAACAAATAG

Upstream 100 bases:

>100_bases
ATAAATCTAATTATAAATTTATTTATAAACAAAAATATTTAAATTTATATCATAAAAGAAACTAAAATTAAAATAACTAA
TTATAAGGAATTATTTTATT

Downstream 100 bases:

>100_bases
CTAAATTTTAACTTTTTTTTAAATTTAAATCCTTTTTACAATAAAAAAAAGAAGAAAAAAGTCCAGATTTTAAAAATCTA
GAGATAATTATCTGCCTTTT

Product: thymidylate synthase, ThyA

Products: dihydrofolate; dTMP

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MLSINQCYLDFVKKILNQGKETYKDSNHHLKESLGNFYVIDDPLNLKFVAKYQHMTTDMMLNEIKSGKYDIEGCPIKSDA
LYEYVKSFENSDDQGFVYTYPNRILAHFDVDQFEVMKKRMLHAIGSNRAVAVTYDPKLDQDREDIPCLQFLQCIVRNNEL
TIHCLFRSNDIFGAFYSNMFFITYIGIKMKEELNKELLGEKVNFGGIHYHSTSGHIYSNDMKAAKKLIAINK

Sequences:

>Translated_232_residues
MLSINQCYLDFVKKILNQGKETYKDSNHHLKESLGNFYVIDDPLNLKFVAKYQHMTTDMMLNEIKSGKYDIEGCPIKSDA
LYEYVKSFENSDDQGFVYTYPNRILAHFDVDQFEVMKKRMLHAIGSNRAVAVTYDPKLDQDREDIPCLQFLQCIVRNNEL
TIHCLFRSNDIFGAFYSNMFFITYIGIKMKEELNKELLGEKVNFGGIHYHSTSGHIYSNDMKAAKKLIAINK
>Mature_232_residues
MLSINQCYLDFVKKILNQGKETYKDSNHHLKESLGNFYVIDDPLNLKFVAKYQHMTTDMMLNEIKSGKYDIEGCPIKSDA
LYEYVKSFENSDDQGFVYTYPNRILAHFDVDQFEVMKKRMLHAIGSNRAVAVTYDPKLDQDREDIPCLQFLQCIVRNNEL
TIHCLFRSNDIFGAFYSNMFFITYIGIKMKEELNKELLGEKVNFGGIHYHSTSGHIYSNDMKAAKKLIAINK

Specific function: May catalyze the biosynthesis of dTMP using an unknown cosubstrate. In vitro, also catalyzes the dehalogenation of 5- bromo-deoxyuridine monophosphate (Br-dUMP) and the tritium exchange of [5-3H]deoxyuridine monophosphate ([5-3H]dUMP) [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Archaeal- type thyA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR014620
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: 2.1.1.45

Molecular weight: Translated: 26976; Mature: 26976

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSINQCYLDFVKKILNQGKETYKDSNHHLKESLGNFYVIDDPLNLKFVAKYQHMTTDMM
CCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHCCCEEEEECCCCEEEEEHHHHHHHHHH
LNEIKSGKYDIEGCPIKSDALYEYVKSFENSDDQGFVYTYPNRILAHFDVDQFEVMKKRM
HHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCHHHHHHHHHH
LHAIGSNRAVAVTYDPKLDQDREDIPCLQFLQCIVRNNELTIHCLFRSNDIFGAFYSNMF
HHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHCEE
FITYIGIKMKEELNKELLGEKVNFGGIHYHSTSGHIYSNDMKAAKKLIAINK
EEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCHHHHHHHHHHCCC
>Mature Secondary Structure
MLSINQCYLDFVKKILNQGKETYKDSNHHLKESLGNFYVIDDPLNLKFVAKYQHMTTDMM
CCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHCCCEEEEECCCCEEEEEHHHHHHHHHH
LNEIKSGKYDIEGCPIKSDALYEYVKSFENSDDQGFVYTYPNRILAHFDVDQFEVMKKRM
HHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCHHHHHHHHHH
LHAIGSNRAVAVTYDPKLDQDREDIPCLQFLQCIVRNNELTIHCLFRSNDIFGAFYSNMF
HHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHCEE
FITYIGIKMKEELNKELLGEKVNFGGIHYHSTSGHIYSNDMKAAKKLIAINK
EEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,10-methylenetetrahydrofolate; dUMP

Specific reaction: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617278; 8143733 [H]