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The map label for this gene is trpB2 [H]

Identifier: 148643302

GI number: 148643302

Start: 1246482

End: 1247783

Strand: Reverse

Name: trpB2 [H]

Synonym: Msm_1242

Alternate gene names: 148643302

Gene position: 1247783-1246482 (Counterclockwise)

Preceding gene: 148643305

Following gene: 148643301

Centisome position: 67.33

GC content: 36.1

Gene sequence:

>1302_bases
ATGAATTATAAAATAGAATTATCCTCTGAAGAAGTGCCAAAAAAATGGTACAATATTAACGCTGATTTACCTTGTGAACT
TCCAATGCCAAAAAATAGTGAAGGAAGAAATCAAATTGAAGATTTGCAAAAAGCATTTACAAAAGCGGCACTGGACCAGG
AGTTTGCAACTGAAAGATACATTAAGATTCCACAAAAAGTAAGGGAGTTATACATGCAGATGGGAAGGCCAACTCCGTTA
TTTAGAGCTAAACGTCTTGAAGAAAAGCTCAACACTCCAGCTAAAATTTATTACAAAAGGGAAGATACTTCTCCTACGGG
ATCACATAAATTAAACAGTGCAATTCCACAGGCATACTTTGCTAAAAAAGAAGGTGTTGAAAGATTAACAACCGAAACTG
GTGCAGGACAATGGGGAACTGCTTTGTCACTTGCTTGTTCATTACTTGATTTGGACTGTACTGTATATATGGTTAAAGTA
TCATTTAATCAAAAGCCAGACAGGAAAAACATCATGAATATTTATGGCGGAGAAGTCTTTGCCTCCCCAAGTGAAAATAC
AGAATTTGGACGTAAAGTATTGGCTGAAAACCCTGACCATGTAGGATCACTTGGAATAGCTATTTCAGAAGCAATGGAAG
AAGCTCTAAACAATGACAATGTAAAATACACTTTAGGCAGTGTGTTAAACCATGTAATGTTACATCAAACTGTCATCGGT
CAGGAACTTAAAACACAATTGGAAATAGCTAATGAAGAACCAGACACTATGATTGCATGTGTTGGTGGTGGAAGTAACTT
TGCAGGTGCTTTATATCCATTTATTAAAGATAAACTTGATGGAAACAGTGATACTAAATTTATTGCAGTAGAACCGAGTG
CATGTCCAACATTAACTGAAGGTAAATATGAATACGACTTTGGAGATTCAAATGGATTTACTCCACTTCTTAAAATGTAT
ACCTTAGGCCATGACTTTGTAGCACCATCTGTACATGCAGGAGGTTTAAGATACCATGGAATGTGTCCACAAATCTCATT
ACTTGCACATGAAGGATATATCAATCCTGTAACAGCTCATCAAAGAGACGTATTTAATGCAGGAATTCAATTTGCTAAAT
GTGAAGGAATTGTTCCGGCACCGGAAACTACTCATGCAATTAAGGCAGGTATTGATGAAGCTATTAAATGCAGACAAACT
GGAGAAGAAAAAACCATTGTTATTAACTTCTCAGGACATGGTATGCTGGATTTAAAAGGTTATGCAAATTATTTCTCTGG
TGAAATGCCAAACAGTAAATAG

Upstream 100 bases:

>100_bases
AAATTTTCATATTTCTAAATAGGTTTTTATTCTATTAAAATATTAATTGTACAAAAGTGTACAGTAAAGTTTTAAATAGT
ACATTATACAATCTATTAAT

Downstream 100 bases:

>100_bases
AGTGAGATTAATTCTCTACTCTTTCTTTATTTTTTTGAATATTTTAATTTAAGAATTGCTTCTGTATTTAGATATGACCT
GTTTTTTAGATGTAAAATTA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNYKIELSSEEVPKKWYNINADLPCELPMPKNSEGRNQIEDLQKAFTKAALDQEFATERYIKIPQKVRELYMQMGRPTPL
FRAKRLEEKLNTPAKIYYKREDTSPTGSHKLNSAIPQAYFAKKEGVERLTTETGAGQWGTALSLACSLLDLDCTVYMVKV
SFNQKPDRKNIMNIYGGEVFASPSENTEFGRKVLAENPDHVGSLGIAISEAMEEALNNDNVKYTLGSVLNHVMLHQTVIG
QELKTQLEIANEEPDTMIACVGGGSNFAGALYPFIKDKLDGNSDTKFIAVEPSACPTLTEGKYEYDFGDSNGFTPLLKMY
TLGHDFVAPSVHAGGLRYHGMCPQISLLAHEGYINPVTAHQRDVFNAGIQFAKCEGIVPAPETTHAIKAGIDEAIKCRQT
GEEKTIVINFSGHGMLDLKGYANYFSGEMPNSK

Sequences:

>Translated_433_residues
MNYKIELSSEEVPKKWYNINADLPCELPMPKNSEGRNQIEDLQKAFTKAALDQEFATERYIKIPQKVRELYMQMGRPTPL
FRAKRLEEKLNTPAKIYYKREDTSPTGSHKLNSAIPQAYFAKKEGVERLTTETGAGQWGTALSLACSLLDLDCTVYMVKV
SFNQKPDRKNIMNIYGGEVFASPSENTEFGRKVLAENPDHVGSLGIAISEAMEEALNNDNVKYTLGSVLNHVMLHQTVIG
QELKTQLEIANEEPDTMIACVGGGSNFAGALYPFIKDKLDGNSDTKFIAVEPSACPTLTEGKYEYDFGDSNGFTPLLKMY
TLGHDFVAPSVHAGGLRYHGMCPQISLLAHEGYINPVTAHQRDVFNAGIQFAKCEGIVPAPETTHAIKAGIDEAIKCRQT
GEEKTIVINFSGHGMLDLKGYANYFSGEMPNSK
>Mature_433_residues
MNYKIELSSEEVPKKWYNINADLPCELPMPKNSEGRNQIEDLQKAFTKAALDQEFATERYIKIPQKVRELYMQMGRPTPL
FRAKRLEEKLNTPAKIYYKREDTSPTGSHKLNSAIPQAYFAKKEGVERLTTETGAGQWGTALSLACSLLDLDCTVYMVKV
SFNQKPDRKNIMNIYGGEVFASPSENTEFGRKVLAENPDHVGSLGIAISEAMEEALNNDNVKYTLGSVLNHVMLHQTVIG
QELKTQLEIANEEPDTMIACVGGGSNFAGALYPFIKDKLDGNSDTKFIAVEPSACPTLTEGKYEYDFGDSNGFTPLLKMY
TLGHDFVAPSVHAGGLRYHGMCPQISLLAHEGYINPVTAHQRDVFNAGIQFAKCEGIVPAPETTHAIKAGIDEAIKCRQT
GEEKTIVINFSGHGMLDLKGYANYFSGEMPNSK

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=402, Percent_Identity=30.0995024875622, Blast_Score=122, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321412, Length=403, Percent_Identity=31.5136476426799, Blast_Score=152, Evalue=8e-38,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 47929; Mature: 47929

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKIELSSEEVPKKWYNINADLPCELPMPKNSEGRNQIEDLQKAFTKAALDQEFATERY
CCEEEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IKIPQKVRELYMQMGRPTPLFRAKRLEEKLNTPAKIYYKREDTSPTGSHKLNSAIPQAYF
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
AKKEGVERLTTETGAGQWGTALSLACSLLDLDCTVYMVKVSFNQKPDRKNIMNIYGGEVF
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHCCCEEE
ASPSENTEFGRKVLAENPDHVGSLGIAISEAMEEALNNDNVKYTLGSVLNHVMLHQTVIG
ECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
QELKTQLEIANEEPDTMIACVGGGSNFAGALYPFIKDKLDGNSDTKFIAVEPSACPTLTE
HHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GKYEYDFGDSNGFTPLLKMYTLGHDFVAPSVHAGGLRYHGMCPQISLLAHEGYINPVTAH
CEEEECCCCCCCCHHHHHHHHCCCHHCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCHH
QRDVFNAGIQFAKCEGIVPAPETTHAIKAGIDEAIKCRQTGEEKTIVINFSGHGMLDLKG
HHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECH
YANYFSGEMPNSK
HHHHHCCCCCCCC
>Mature Secondary Structure
MNYKIELSSEEVPKKWYNINADLPCELPMPKNSEGRNQIEDLQKAFTKAALDQEFATERY
CCEEEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IKIPQKVRELYMQMGRPTPLFRAKRLEEKLNTPAKIYYKREDTSPTGSHKLNSAIPQAYF
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
AKKEGVERLTTETGAGQWGTALSLACSLLDLDCTVYMVKVSFNQKPDRKNIMNIYGGEVF
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHCCCEEE
ASPSENTEFGRKVLAENPDHVGSLGIAISEAMEEALNNDNVKYTLGSVLNHVMLHQTVIG
ECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
QELKTQLEIANEEPDTMIACVGGGSNFAGALYPFIKDKLDGNSDTKFIAVEPSACPTLTE
HHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GKYEYDFGDSNGFTPLLKMYTLGHDFVAPSVHAGGLRYHGMCPQISLLAHEGYINPVTAH
CEEEECCCCCCCCHHHHHHHHCCCHHCCCCCCCCCEEECCCCCCEEEEEECCCCCCCCHH
QRDVFNAGIQFAKCEGIVPAPETTHAIKAGIDEAIKCRQTGEEKTIVINFSGHGMLDLKG
HHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECH
YANYFSGEMPNSK
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]