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The map label for this gene is ilvB [H]

Identifier: 148643285

GI number: 148643285

Start: 1225160

End: 1226851

Strand: Reverse

Name: ilvB [H]

Synonym: Msm_1225

Alternate gene names: 148643285

Gene position: 1226851-1225160 (Counterclockwise)

Preceding gene: 148643286

Following gene: 148643284

Centisome position: 66.2

GC content: 34.87

Gene sequence:

>1692_bases
ATGAGAGGCGGAGAAGCAATAATTGAATCCCTAAAAAGAATGGGGGTAAATACAATCTTTGGTTATCCTGGAGGACAAAC
CATACCGTTTTATGACATGTTATATGATGCAGACATGGATCATATATTGGTTAGACATGAACAGTGTGCAGCTCATGCAG
CAGATGGATTTGCAAGAGCTTCTGGTAAAGTGGGTGTGTGTTTGGCTACTTCTGGCCCTGGTGCAACTAATCTTGTAACC
GGTATTGCTACTGCTTATATGGATTCTTCTCCAATCATAGCTATTACTGGTCAAGTACCAACACATTTAATAGGTAATGA
CGCTTTTCAAGAAGCTGATATTATAGGAATTACTATGCCTATTACTAAACACAGTTTCCAGCCAAAAGATCCTAATTTAA
TACCTTCCATTGTTAAAACAAGCTTTGAAATAGCTGAAAGTGGAAGGCCGGGTCCGGTTTTAATTGATGTTCCAAAAGAA
ATTCAGGAAGGAGAATTGGATTCTTTTGATGATTCATTAATTTCAACACCAGGTTATAATCCAACTTTAAAAGGAAACAT
TAAACAAGTTAGAAAAGCCCGTGATTTAATAAGGGAATCTAAAAAACCGATTATCCTTGCAGGTGCAGGAGTTATATTAG
CTAATGCTTCACAGGAGTTGAAAGAATTATCTTACTTAATTAATGCTCCGGTCATGACATCATTACTTGGTAAAGGAGCT
ATTGATGAAACAGATGATATGGCATTGGGCATGCTTGGAATGCATGGAAGAAAAGTAGCTAATGATTCAATAAATGAAAG
TGACTTGATTATAGCTGTAGGTATAAGATTTTCAGACAGGGCAACAGGAAGATTAGACAGTTTTGCACCTAATTCTAAAA
TTATCCATATAGACATTGATCCTGCTGAAATAGGTAAAAATGTAGATGTTGATTTGCCTATTGTAGGGGATGCTAAAAAT
GTTTTATCTTCATTAAATAATGTTTTAAAAGATTATGAAAGTAACGAAGATGCAAAAAAATGGGTTAAACTTCTTATAGA
ACGTAAAAAAGAATGTTTCCCTAGAGTTACTTATGATGATATTCCATTAAAACCACAAAGTGTTATCCGAGAAATCAGTG
AAGTTTTAACTCCTGATTCTATTTTAACAACTGATGTAGGTCAAAATCAAATGTGGGCGGCTCATTTCTATGATACTCAA
AAGCCACGTAAATTCATATCTTCCGGGGGTTTGGGAACTATGGGATTCGGATTGCCTGCAGCTATTGGAGCTAAAGTAGC
ATGTCCTGAAGATCCTGTTGTATCCATTAATGGTGACGGAGGATTTTTAATGGTTTGTCAGGAGTTAGCTACTATTAAAG
ATTATGATATTCCTGTTATTGCAGTTGTTTTAGAAAATAGAACTCTTGGAATGGTTTATCAATGGCAAAACTTATTATAT
AATGGAAGATATTCTGAAACTAAATTAGGAACAAGTCCTGATTTTGTTAAATTAGCTGAAAGTTTTGGAGTTAATGCAAC
CAGAATTACAAAACCTGGAGAAACTAAAGAGGCATTAAAATCAGCTATTAAGGATAATGAACCTATTTTGCTTGATGTTA
TAATTGATAAAGATGAAACTTTACCGATGGTGCCGCCAGGTGCAGCTATTGATGAAATGATTGGTGAATATAAACTTGAA
AAAGATGTATAG

Upstream 100 bases:

>100_bases
ATTTTAATTTTCATTATTTTTTCTTAAAAAACTAAGATACTTTTATATATTTTCATTAATAAACTTTAATCATTAAATAA
AAAAAATTAAGGTTTTTATA

Downstream 100 bases:

>100_bases
GTGAGTTAAATGGCTTTAGAATATCATGTTATAAATGCATTAGTTGAAGATAAGCCGGGAGTATTGCAAAAAGTTGCTGG
CCTATTTACAAGAAGAGGTT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MRGGEAIIESLKRMGVNTIFGYPGGQTIPFYDMLYDADMDHILVRHEQCAAHAADGFARASGKVGVCLATSGPGATNLVT
GIATAYMDSSPIIAITGQVPTHLIGNDAFQEADIIGITMPITKHSFQPKDPNLIPSIVKTSFEIAESGRPGPVLIDVPKE
IQEGELDSFDDSLISTPGYNPTLKGNIKQVRKARDLIRESKKPIILAGAGVILANASQELKELSYLINAPVMTSLLGKGA
IDETDDMALGMLGMHGRKVANDSINESDLIIAVGIRFSDRATGRLDSFAPNSKIIHIDIDPAEIGKNVDVDLPIVGDAKN
VLSSLNNVLKDYESNEDAKKWVKLLIERKKECFPRVTYDDIPLKPQSVIREISEVLTPDSILTTDVGQNQMWAAHFYDTQ
KPRKFISSGGLGTMGFGLPAAIGAKVACPEDPVVSINGDGGFLMVCQELATIKDYDIPVIAVVLENRTLGMVYQWQNLLY
NGRYSETKLGTSPDFVKLAESFGVNATRITKPGETKEALKSAIKDNEPILLDVIIDKDETLPMVPPGAAIDEMIGEYKLE
KDV

Sequences:

>Translated_563_residues
MRGGEAIIESLKRMGVNTIFGYPGGQTIPFYDMLYDADMDHILVRHEQCAAHAADGFARASGKVGVCLATSGPGATNLVT
GIATAYMDSSPIIAITGQVPTHLIGNDAFQEADIIGITMPITKHSFQPKDPNLIPSIVKTSFEIAESGRPGPVLIDVPKE
IQEGELDSFDDSLISTPGYNPTLKGNIKQVRKARDLIRESKKPIILAGAGVILANASQELKELSYLINAPVMTSLLGKGA
IDETDDMALGMLGMHGRKVANDSINESDLIIAVGIRFSDRATGRLDSFAPNSKIIHIDIDPAEIGKNVDVDLPIVGDAKN
VLSSLNNVLKDYESNEDAKKWVKLLIERKKECFPRVTYDDIPLKPQSVIREISEVLTPDSILTTDVGQNQMWAAHFYDTQ
KPRKFISSGGLGTMGFGLPAAIGAKVACPEDPVVSINGDGGFLMVCQELATIKDYDIPVIAVVLENRTLGMVYQWQNLLY
NGRYSETKLGTSPDFVKLAESFGVNATRITKPGETKEALKSAIKDNEPILLDVIIDKDETLPMVPPGAAIDEMIGEYKLE
KDV
>Mature_563_residues
MRGGEAIIESLKRMGVNTIFGYPGGQTIPFYDMLYDADMDHILVRHEQCAAHAADGFARASGKVGVCLATSGPGATNLVT
GIATAYMDSSPIIAITGQVPTHLIGNDAFQEADIIGITMPITKHSFQPKDPNLIPSIVKTSFEIAESGRPGPVLIDVPKE
IQEGELDSFDDSLISTPGYNPTLKGNIKQVRKARDLIRESKKPIILAGAGVILANASQELKELSYLINAPVMTSLLGKGA
IDETDDMALGMLGMHGRKVANDSINESDLIIAVGIRFSDRATGRLDSFAPNSKIIHIDIDPAEIGKNVDVDLPIVGDAKN
VLSSLNNVLKDYESNEDAKKWVKLLIERKKECFPRVTYDDIPLKPQSVIREISEVLTPDSILTTDVGQNQMWAAHFYDTQ
KPRKFISSGGLGTMGFGLPAAIGAKVACPEDPVVSINGDGGFLMVCQELATIKDYDIPVIAVVLENRTLGMVYQWQNLLY
NGRYSETKLGTSPDFVKLAESFGVNATRITKPGETKEALKSAIKDNEPILLDVIIDKDETLPMVPPGAAIDEMIGEYKLE
KDV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=24.7386759581882, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI21361361, Length=598, Percent_Identity=25.752508361204, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI87081685, Length=564, Percent_Identity=45.9219858156028, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1790104, Length=557, Percent_Identity=40.394973070018, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=33.8078291814947, Blast_Score=344, Evalue=1e-95,
Organism=Escherichia coli, GI1787096, Length=561, Percent_Identity=29.0552584670232, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1788716, Length=551, Percent_Identity=27.2232304900181, Blast_Score=181, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17531299, Length=558, Percent_Identity=25.9856630824373, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17531301, Length=558, Percent_Identity=25.9856630824373, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=514, Percent_Identity=26.6536964980545, Blast_Score=151, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=42.4870466321244, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6321524, Length=541, Percent_Identity=25.8780036968577, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320816, Length=558, Percent_Identity=23.6559139784946, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=565, Percent_Identity=24.9557522123894, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323073, Length=562, Percent_Identity=25.2669039145907, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320123, Length=491, Percent_Identity=23.8289205702648, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320588, Length=576, Percent_Identity=21.3541666666667, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922626, Length=559, Percent_Identity=25.4025044722719, Blast_Score=180, Evalue=3e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60953; Mature: 60953

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGGEAIIESLKRMGVNTIFGYPGGQTIPFYDMLYDADMDHILVRHEQCAAHAADGFARA
CCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHC
SGKVGVCLATSGPGATNLVTGIATAYMDSSPIIAITGQVPTHLIGNDAFQEADIIGITMP
CCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCHHCCCCEEEEEEC
ITKHSFQPKDPNLIPSIVKTSFEIAESGRPGPVLIDVPKEIQEGELDSFDDSLISTPGYN
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHCCCCCCCCCHHHHCCCCCC
PTLKGNIKQVRKARDLIRESKKPIILAGAGVILANASQELKELSYLINAPVMTSLLGKGA
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCEEEECCHHHHHHHHHHHCCHHHHHHHCCCC
IDETDDMALGMLGMHGRKVANDSINESDLIIAVGIRFSDRATGRLDSFAPNSKIIHIDID
CCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECC
PAEIGKNVDVDLPIVGDAKNVLSSLNNVLKDYESNEDAKKWVKLLIERKKECFPRVTYDD
HHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
IPLKPQSVIREISEVLTPDSILTTDVGQNQMWAAHFYDTQKPRKFISSGGLGTMGFGLPA
CCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHH
AIGAKVACPEDPVVSINGDGGFLMVCQELATIKDYDIPVIAVVLENRTLGMVYQWQNLLY
HHCCEEECCCCCEEEEECCCCEEHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEHHHHHC
NGRYSETKLGTSPDFVKLAESFGVNATRITKPGETKEALKSAIKDNEPILLDVIIDKDET
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCC
LPMVPPGAAIDEMIGEYKLEKDV
CCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRGGEAIIESLKRMGVNTIFGYPGGQTIPFYDMLYDADMDHILVRHEQCAAHAADGFARA
CCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHC
SGKVGVCLATSGPGATNLVTGIATAYMDSSPIIAITGQVPTHLIGNDAFQEADIIGITMP
CCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCHHCCCCEEEEEEC
ITKHSFQPKDPNLIPSIVKTSFEIAESGRPGPVLIDVPKEIQEGELDSFDDSLISTPGYN
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHCCCCCCCCCHHHHCCCCCC
PTLKGNIKQVRKARDLIRESKKPIILAGAGVILANASQELKELSYLINAPVMTSLLGKGA
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCEEEECCHHHHHHHHHHHCCHHHHHHHCCCC
IDETDDMALGMLGMHGRKVANDSINESDLIIAVGIRFSDRATGRLDSFAPNSKIIHIDID
CCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECC
PAEIGKNVDVDLPIVGDAKNVLSSLNNVLKDYESNEDAKKWVKLLIERKKECFPRVTYDD
HHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
IPLKPQSVIREISEVLTPDSILTTDVGQNQMWAAHFYDTQKPRKFISSGGLGTMGFGLPA
CCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHH
AIGAKVACPEDPVVSINGDGGFLMVCQELATIKDYDIPVIAVVLENRTLGMVYQWQNLLY
HHCCEEECCCCCEEEEECCCCEEHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEHHHHHC
NGRYSETKLGTSPDFVKLAESFGVNATRITKPGETKEALKSAIKDNEPILLDVIIDKDET
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCC
LPMVPPGAAIDEMIGEYKLEKDV
CCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]