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The map label for this gene is argF

Identifier: 148643286

GI number: 148643286

Start: 1226963

End: 1227868

Strand: Reverse

Name: argF

Synonym: Msm_1226

Alternate gene names: 148643286

Gene position: 1227868-1226963 (Counterclockwise)

Preceding gene: 148643287

Following gene: 148643285

Centisome position: 66.26

GC content: 34.22

Gene sequence:

>906_bases
ATGAGCAATTTATTGTCTGTTTGTGACATCAAAGATGAAGTACTGGATATTTTAGAATTAGCTAAAAATTTCAAAGAAGG
AAAAATGGAAGAAAAACCTTTAGCAGGTAAATCATTAGCTATGATATTTCAAAAATCATCTACAAGGACTAGGGTATCTT
TTGATGTAGGTATGTATCAGTTAGGTGGTCAGGCATTGTTTTTATCCTCTTCAGAATTACAAATGGGTAGAGGAGAACCT
ATTCCGGATACTGCAAAAGTTTTAAGTCGTTTTGTAGATGGAATAATGATACGTGCTATTGAACATGATGATGTTGTGGA
ATTAGCTAAATATTCTGATGTTCCGGTTATCAGTGGTTTAACTAACTTGGAACACCCTTGCCAAGCTTTAGCTGATATGT
TAACTATTCAGGAACATTTGGGTAAATTGGAAGGCAAAAAACTTTGTTTTGTTGGTGATGGAAACAATGTATGTAATTCT
TTGCTTTTAATGGCACCTCTTGTTGGAATGGACATGTCTGTAGCATGCCCTGAAGGTTATGAACCTAACGAAGACATTGT
TAATATGGCTAAAAAATTAGCAGAAGAACATAACAAGGAAATTACTATTAGTTCTGATTTAAAAGTCGCTCTTGATAATG
TGGATGTAGTCTATACTGATGTTTGGGTAAGTATGGGTGATGAAAAAGAAGCAGAACAAAGACAAAAAGATTTTGCACCT
TATCAAGTTAATTCTAATTTAATGAGTTTAGCTAATGATGGGGCTATTTTTATGCATTGCTTACCGGCTATTCGTGGTCA
GGAAGTTAGTGGTGAAGTTATTGATGGTCCTCAATCAGTGGTCTTTGATGAAGCTGAAAACAGACTTCATGCACAAAAAG
CAGTTCTTTATCACTTTTTAAAATAA

Upstream 100 bases:

>100_bases
ATGCTTTTATTTCTGTTTGTTATATTAAATGGTATAGAAAACTTTAATATATAATAATTATATATATTTTATTTTGTTAA
GATTAAAGAAGGGTTAAACT

Downstream 100 bases:

>100_bases
CTTTTTTTCAAATTTTAATTTTCATTATTTTTTCTTAAAAAACTAAGATACTTTTATATATTTTCATTAATAAACTTTAA
TCATTAAATAAAAAAAATTA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSNLLSVCDIKDEVLDILELAKNFKEGKMEEKPLAGKSLAMIFQKSSTRTRVSFDVGMYQLGGQALFLSSSELQMGRGEP
IPDTAKVLSRFVDGIMIRAIEHDDVVELAKYSDVPVISGLTNLEHPCQALADMLTIQEHLGKLEGKKLCFVGDGNNVCNS
LLLMAPLVGMDMSVACPEGYEPNEDIVNMAKKLAEEHNKEITISSDLKVALDNVDVVYTDVWVSMGDEKEAEQRQKDFAP
YQVNSNLMSLANDGAIFMHCLPAIRGQEVSGEVIDGPQSVVFDEAENRLHAQKAVLYHFLK

Sequences:

>Translated_301_residues
MSNLLSVCDIKDEVLDILELAKNFKEGKMEEKPLAGKSLAMIFQKSSTRTRVSFDVGMYQLGGQALFLSSSELQMGRGEP
IPDTAKVLSRFVDGIMIRAIEHDDVVELAKYSDVPVISGLTNLEHPCQALADMLTIQEHLGKLEGKKLCFVGDGNNVCNS
LLLMAPLVGMDMSVACPEGYEPNEDIVNMAKKLAEEHNKEITISSDLKVALDNVDVVYTDVWVSMGDEKEAEQRQKDFAP
YQVNSNLMSLANDGAIFMHCLPAIRGQEVSGEVIDGPQSVVFDEAENRLHAQKAVLYHFLK
>Mature_300_residues
SNLLSVCDIKDEVLDILELAKNFKEGKMEEKPLAGKSLAMIFQKSSTRTRVSFDVGMYQLGGQALFLSSSELQMGRGEPI
PDTAKVLSRFVDGIMIRAIEHDDVVELAKYSDVPVISGLTNLEHPCQALADMLTIQEHLGKLEGKKLCFVGDGNNVCNSL
LLMAPLVGMDMSVACPEGYEPNEDIVNMAKKLAEEHNKEITISSDLKVALDNVDVVYTDVWVSMGDEKEAEQRQKDFAPY
QVNSNLMSLANDGAIFMHCLPAIRGQEVSGEVIDGPQSVVFDEAENRLHAQKAVLYHFLK

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=293, Percent_Identity=39.9317406143345, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=27.9605263157895, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1790703, Length=310, Percent_Identity=41.6129032258065, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1786469, Length=301, Percent_Identity=41.8604651162791, Blast_Score=220, Evalue=9e-59,
Organism=Escherichia coli, GI48994908, Length=328, Percent_Identity=27.1341463414634, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI2367364, Length=303, Percent_Identity=27.7227722772277, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193204318, Length=318, Percent_Identity=27.0440251572327, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322373, Length=319, Percent_Identity=36.0501567398119, Blast_Score=197, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6322331, Length=269, Percent_Identity=30.4832713754647, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24642586, Length=294, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_METS3 (A5UMK3)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273799.1
- ProteinModelPortal:   A5UMK3
- SMR:   A5UMK3
- STRING:   A5UMK3
- GeneID:   5216056
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_1226
- eggNOG:   arNOG04256
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- ProtClustDB:   PRK00779
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33196; Mature: 33065

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 98-98 BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLLSVCDIKDEVLDILELAKNFKEGKMEEKPLAGKSLAMIFQKSSTRTRVSFDVGMYQ
CCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEECCCCCEEEEEECCHHH
LGGQALFLSSSELQMGRGEPIPDTAKVLSRFVDGIMIRAIEHDDVVELAKYSDVPVISGL
HCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCCCHHHCC
TNLEHPCQALADMLTIQEHLGKLEGKKLCFVGDGNNVCNSLLLMAPLVGMDMSVACPEGY
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCC
EPNEDIVNMAKKLAEEHNKEITISSDLKVALDNVDVVYTDVWVSMGDEKEAEQRQKDFAP
CCCHHHHHHHHHHHHHCCCEEEEECCCEEEECCCEEEEEEEEEECCCHHHHHHHHHCCCC
YQVNSNLMSLANDGAIFMHCLPAIRGQEVSGEVIDGPQSVVFDEAENRLHAQKAVLYHFL
EEECCHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
K
C
>Mature Secondary Structure 
SNLLSVCDIKDEVLDILELAKNFKEGKMEEKPLAGKSLAMIFQKSSTRTRVSFDVGMYQ
CCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEECCCCCEEEEEECCHHH
LGGQALFLSSSELQMGRGEPIPDTAKVLSRFVDGIMIRAIEHDDVVELAKYSDVPVISGL
HCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCCCHHHCC
TNLEHPCQALADMLTIQEHLGKLEGKKLCFVGDGNNVCNSLLLMAPLVGMDMSVACPEGY
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCC
EPNEDIVNMAKKLAEEHNKEITISSDLKVALDNVDVVYTDVWVSMGDEKEAEQRQKDFAP
CCCHHHHHHHHHHHHHCCCEEEEECCCEEEECCCEEEEEEEEEECCCHHHHHHHHHCCCC
YQVNSNLMSLANDGAIFMHCLPAIRGQEVSGEVIDGPQSVVFDEAENRLHAQKAVLYHFL
EEECCHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA