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The map label for this gene is eIF2A

Identifier: 148643193

GI number: 148643193

Start: 1112001

End: 1112798

Strand: Reverse

Name: eIF2A

Synonym: Msm_1133

Alternate gene names: 148643193

Gene position: 1112798-1112001 (Counterclockwise)

Preceding gene: 148643194

Following gene: 148643192

Centisome position: 60.05

GC content: 35.21

Gene sequence:

>798_bases
ATGGTAAGGAAAAATCAAGAATGGCCTGACGAAGGAGAACTTATTATTGGTACTGTTTATAAAGTTCTTAACTATGGGGC
TTTCGCAAAATTAGAAGAATACCATGGTAAAGAAGCTTTTATTCACATTTCTGAGGTATCTTCTGGTTGGGTAAAAAATA
TCAGAGACCATGTAAGAGAAAATCAAAAAATAGTTTGCCGTGTTCTTAGAGTAAATCCTAAAAAAGGACATGTAGATGCT
TCTTTAAAAAGAATTAGGGAAGATCAAAGAACTAAAAAAATCCAGCATTGGAAAATTGAACAAAAAGCTGAAAAATTCCT
GGAATTATCTGCTAAATCATTAGGCAAATCTTTAAATGATGCTTACGATGAAGTAGGTTATGAGCTAATGGATATTTTCG
GTGATGTTTACGGTGCTTTTGAAACAGCAGCTGATGATGGTGCAAAATCTCTTACTGATGAAGGTATATCTCAAGAGTGG
GCTGATGCTATAACTGAAATAGCTAATAAAAATATTACTCCTCCTGAAGTTCATATCAGCGGGTATGTTGATATTGAAAC
TTTCGTCCCGGATGGTGTTGATGTCATTATTGAAGCTCTTAAAGCAGCTGAAGATAATGGTGACGAAGAGGAAGAAATCA
AGGTTCAATGTGTTGGTGCACCAAGATATAGGATAACTGTAAAATCCACTGATTACATTTTGGCTGAAAAAGCTCTTAAA
GCAGCAGCTGACAGATGTATTGAAATAGTTGAAGCTTCTGAAGGAAATGGTTCTTTCTTAAGAGAGTTGGATAGCTAG

Upstream 100 bases:

>100_bases
TATTGATAAAGTTTTAAACTAGATTCAATCGAAATTTTTTAATCTAGCTTATTATTTTTTCTATTTTTTTTTAAATTTTT
ATTTTATTTTTGGTGATTAT

Downstream 100 bases:

>100_bases
ATTTTTATGAATATGAAAATGAACAAATGTCCTGATTGTAAAATTTATACAATGGAGGATGCTTGCCCTCAATGTGGCGG
TGAGCTTAAAGTTATTTATC

Product: translation initiation factor IF-2 subunit alpha

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: aIF2-alpha; eIF-2-alpha

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MVRKNQEWPDEGELIIGTVYKVLNYGAFAKLEEYHGKEAFIHISEVSSGWVKNIRDHVRENQKIVCRVLRVNPKKGHVDA
SLKRIREDQRTKKIQHWKIEQKAEKFLELSAKSLGKSLNDAYDEVGYELMDIFGDVYGAFETAADDGAKSLTDEGISQEW
ADAITEIANKNITPPEVHISGYVDIETFVPDGVDVIIEALKAAEDNGDEEEEIKVQCVGAPRYRITVKSTDYILAEKALK
AAADRCIEIVEASEGNGSFLRELDS

Sequences:

>Translated_265_residues
MVRKNQEWPDEGELIIGTVYKVLNYGAFAKLEEYHGKEAFIHISEVSSGWVKNIRDHVRENQKIVCRVLRVNPKKGHVDA
SLKRIREDQRTKKIQHWKIEQKAEKFLELSAKSLGKSLNDAYDEVGYELMDIFGDVYGAFETAADDGAKSLTDEGISQEW
ADAITEIANKNITPPEVHISGYVDIETFVPDGVDVIIEALKAAEDNGDEEEEIKVQCVGAPRYRITVKSTDYILAEKALK
AAADRCIEIVEASEGNGSFLRELDS
>Mature_265_residues
MVRKNQEWPDEGELIIGTVYKVLNYGAFAKLEEYHGKEAFIHISEVSSGWVKNIRDHVRENQKIVCRVLRVNPKKGHVDA
SLKRIREDQRTKKIQHWKIEQKAEKFLELSAKSLGKSLNDAYDEVGYELMDIFGDVYGAFETAADDGAKSLTDEGISQEW
ADAITEIANKNITPPEVHISGYVDIETFVPDGVDVIIEALKAAEDNGDEEEEIKVQCVGAPRYRITVKSTDYILAEKALK
AAADRCIEIVEASEGNGSFLRELDS

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA

COG id: COG1093

COG function: function code J; Translation initiation factor 2, alpha subunit (eIF-2alpha)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI4758256, Length=241, Percent_Identity=24.4813278008299, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17509871, Length=244, Percent_Identity=25.8196721311475, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322466, Length=247, Percent_Identity=28.7449392712551, Blast_Score=112, Evalue=9e-26,
Organism=Drosophila melanogaster, GI18860087, Length=274, Percent_Identity=24.0875912408759, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): IF2A_METS3 (A5UMB0)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273706.1
- ProteinModelPortal:   A5UMB0
- SMR:   A5UMB0
- STRING:   A5UMB0
- GeneID:   5217536
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_1133
- eggNOG:   arNOG06306
- HOGENOM:   HBG496806
- OMA:   EYPGKEG
- ProtClustDB:   PRK03987
- HAMAP:   MF_00231
- InterPro:   IPR011488
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR022964
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00316

Pfam domain/function: PF07541 EIF_2_alpha; PF00575 S1; SSF50249 Nucleic_acid_OB

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 29761; Mature: 29761

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKNQEWPDEGELIIGTVYKVLNYGAFAKLEEYHGKEAFIHISEVSSGWVKNIRDHVRE
CCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEEEEHHHCCHHHHHHHHHHHC
NQKIVCRVLRVNPKKGHVDASLKRIREDQRTKKIQHWKIEQKAEKFLELSAKSLGKSLND
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYDEVGYELMDIFGDVYGAFETAADDGAKSLTDEGISQEWADAITEIANKNITPPEVHIS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEE
GYVDIETFVPDGVDVIIEALKAAEDNGDEEEEIKVQCVGAPRYRITVKSTDYILAEKALK
EEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCHHHHHHHHH
AAADRCIEIVEASEGNGSFLRELDS
HHHHHHHHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MVRKNQEWPDEGELIIGTVYKVLNYGAFAKLEEYHGKEAFIHISEVSSGWVKNIRDHVRE
CCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEEEEHHHCCHHHHHHHHHHHC
NQKIVCRVLRVNPKKGHVDASLKRIREDQRTKKIQHWKIEQKAEKFLELSAKSLGKSLND
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYDEVGYELMDIFGDVYGAFETAADDGAKSLTDEGISQEWADAITEIANKNITPPEVHIS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEE
GYVDIETFVPDGVDVIIEALKAAEDNGDEEEEIKVQCVGAPRYRITVKSTDYILAEKALK
EEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCHHHHHHHHH
AAADRCIEIVEASEGNGSFLRELDS
HHHHHHHHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA