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The map label for this gene is cofE [H]

Identifier: 148643035

GI number: 148643035

Start: 936848

End: 937621

Strand: Reverse

Name: cofE [H]

Synonym: Msm_0975

Alternate gene names: 148643035

Gene position: 937621-936848 (Counterclockwise)

Preceding gene: 148643036

Following gene: 148643034

Centisome position: 50.6

GC content: 34.63

Gene sequence:

>774_bases
ATGATTAATATGTCAATAGAATTAATTGGTTTAGACAATATTCCATTAGTTGAACCTAATGACGATGTTTGTCAAATAAT
TAAAGATGCAATTGAAAAACAAGGTTGTTTATTAAAACATGGGGATATTATTTTAATAGCTGAAACATTAATTTCAAAAG
CAGAAGGTAATGTTATCAAATTGGGGGACATAATTCCATCTGAAGAATCCATTATCATTGCTAAACAGTCCAAAAAAGAT
TCTAAATTGGTTGAAGCTATTATAAATGAATCTAGAGAAGTAGTTGCTGTCGGTCCTGATTTTATCATTACTGAAACTAA
ACAGGGTTTTGTTTGTGCAAATTCAGGTATTGACGAATCCAATGTTGATGAAGGATTGGCTACTCCTATGCCTGAAGATG
CTGACAGGTCTGCAAAACAAATAAGGGAATTTTTAGAAAATGAATTTGATGAGGAAGTGGCAGTTTTAATTACTGATACT
CAGGGCAGAGCTTTTAGAAATGGTGCTGTTGGTGTAGCTATTGGATGTTCTGGAATCAATCCGTTATGGAAAAGGGCCGG
CGAAAAAGATTTGTATGGTCGTGAGCTTCAAACTAGTGAAGTAGCTACAGGTGATGAATTGGCAGCCGCAGCTTCTCTTG
TTATGGGTCAGGCTGATGAAGGTCTTCCTGTGGTTATTATCCGAGGATTTGATAATTTTGATAAATTAAGAGATACGAAA
ACCGGAATTAATCCATTACTTAGACCAAAAGAATTTGACGTATTTAGAAATTAG

Upstream 100 bases:

>100_bases
CCAATTACAAAAAAGCAGTAACTGCTGGTGCGGCTGTTTTTGATGGGGAATGGAATATAGGTAATTTCAATCCTTAATTT
TTTTTATTTTTTTTTAAAAG

Downstream 100 bases:

>100_bases
TCTGGAGATTAAATTAATATGATTACTGTACTGTCTGGGGGAACTGGAACTCCAAAATTATTACAAGGTTTAAAAGAAGT
TATTGATCCTAAAGATATTA

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MINMSIELIGLDNIPLVEPNDDVCQIIKDAIEKQGCLLKHGDIILIAETLISKAEGNVIKLGDIIPSEESIIIAKQSKKD
SKLVEAIINESREVVAVGPDFIITETKQGFVCANSGIDESNVDEGLATPMPEDADRSAKQIREFLENEFDEEVAVLITDT
QGRAFRNGAVGVAIGCSGINPLWKRAGEKDLYGRELQTSEVATGDELAAAASLVMGQADEGLPVVIIRGFDNFDKLRDTK
TGINPLLRPKEFDVFRN

Sequences:

>Translated_257_residues
MINMSIELIGLDNIPLVEPNDDVCQIIKDAIEKQGCLLKHGDIILIAETLISKAEGNVIKLGDIIPSEESIIIAKQSKKD
SKLVEAIINESREVVAVGPDFIITETKQGFVCANSGIDESNVDEGLATPMPEDADRSAKQIREFLENEFDEEVAVLITDT
QGRAFRNGAVGVAIGCSGINPLWKRAGEKDLYGRELQTSEVATGDELAAAASLVMGQADEGLPVVIIRGFDNFDKLRDTK
TGINPLLRPKEFDVFRN
>Mature_257_residues
MINMSIELIGLDNIPLVEPNDDVCQIIKDAIEKQGCLLKHGDIILIAETLISKAEGNVIKLGDIIPSEESIIIAKQSKKD
SKLVEAIINESREVVAVGPDFIITETKQGFVCANSGIDESNVDEGLATPMPEDADRSAKQIREFLENEFDEEVAVLITDT
QGRAFRNGAVGVAIGCSGINPLWKRAGEKDLYGRELQTSEVATGDELAAAASLVMGQADEGLPVVIIRGFDNFDKLRDTK
TGINPLLRPKEFDVFRN

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 27936; Mature: 27936

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINMSIELIGLDNIPLVEPNDDVCQIIKDAIEKQGCLLKHGDIILIAETLISKAEGNVIK
CCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCEEEEHHHHHHHCCCCEEE
LGDIIPSEESIIIAKQSKKDSKLVEAIINESREVVAVGPDFIITETKQGFVCANSGIDES
ECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCEEEEECCCCEEEECCCCCCC
NVDEGLATPMPEDADRSAKQIREFLENEFDEEVAVLITDTQGRAFRNGAVGVAIGCSGIN
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEECCCCEEEEEECCCCC
PLWKRAGEKDLYGRELQTSEVATGDELAAAASLVMGQADEGLPVVIIRGFDNFDKLRDTK
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH
TGINPLLRPKEFDVFRN
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MINMSIELIGLDNIPLVEPNDDVCQIIKDAIEKQGCLLKHGDIILIAETLISKAEGNVIK
CCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCEEEEHHHHHHHCCCCEEE
LGDIIPSEESIIIAKQSKKDSKLVEAIINESREVVAVGPDFIITETKQGFVCANSGIDES
ECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCEEEEECCCCEEEECCCCCCC
NVDEGLATPMPEDADRSAKQIREFLENEFDEEVAVLITDTQGRAFRNGAVGVAIGCSGIN
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEECCCCEEEEEECCCCC
PLWKRAGEKDLYGRELQTSEVATGDELAAAASLVMGQADEGLPVVIIRGFDNFDKLRDTK
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH
TGINPLLRPKEFDVFRN
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]